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Open AccessArticle

Polyamidoamide Dendrimers and Cross-Linking Agents for Stabilized Bioenzymatic Resistant Metal-Free Bovine Collagen

1
Department of Molecular Sciences and Nanosystems, University Ca’ Foscari Venice, Via Torino 155, 30172 Mestre (Venice), Italy
2
Crossing Ltd., Viale della Repubblica 193/b, 31100 Treviso, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Paola Vitale
Molecules 2019, 24(19), 3611; https://doi.org/10.3390/molecules24193611
Received: 19 September 2019 / Revised: 30 September 2019 / Accepted: 2 October 2019 / Published: 7 October 2019
(This article belongs to the Special Issue Chemoenzymatic Synthesis and Application)
The work reports the use of polyamidoamine dendrimers (PAMAM) and a cross-linking agent, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide (EDC/NHS) or 4-(4,6-dimethoxy[1,3,5]triazin-2-yl)-4-methyl-morpholinium chloride (DMTMM), for the thermal stabilization of dermal bovine collagen. The efficiency of EDC/NHS/PAMAM and DMTMM/PAMAM in the cross-linking of collagen is correlated to the increase of the collagen shrinkage temperature (Ts), measured by differential scanning calorimetry (DSC). An alternative enzymatic protocol was adopted to measure the degradability of EDC/NHS/PAMAM tanned hides; these data are correlated to the thermal stability values measured by DSC. In the presence of PAMAMs, EDC/NHS provides very high stabilization of bovine dermal collagen, giving Ts of up to 95 °C, while DMTMM achieves lower stabilization. Preliminary tanning tests carried out in best reaction conditions show that EDC/NHS/PAMAM could be an interesting, environmentally-sustainable tanning system which is completely free of metals, formaldehyde, and phenols. Two new unreported dendrimeric species were synthesized and employed. View Full-Text
Keywords: collagen; cross-linking; PAMAM; EDC; enzymatic degradation; DMTMM; dendrimer; tanning collagen; cross-linking; PAMAM; EDC; enzymatic degradation; DMTMM; dendrimer; tanning
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MDPI and ACS Style

Beghetto, V.; Gatto, V.; Conca, S.; Bardella, N.; Scrivanti, A. Polyamidoamide Dendrimers and Cross-Linking Agents for Stabilized Bioenzymatic Resistant Metal-Free Bovine Collagen. Molecules 2019, 24, 3611.

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