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Open AccessArticle

Potentiating Antigen-Specific Antibody Production with Peptides Obtained from In Silico Screening for High-Affinity against MHC-II

1
Structural Physiology Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba 305-8566, Japan
2
Department of Biochemistry, Bio-Peak Co., Ltd., 584-70 Shimonojo, Takasaki 370-0854, Japan
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(16), 2949; https://doi.org/10.3390/molecules24162949
Received: 31 July 2019 / Revised: 10 August 2019 / Accepted: 14 August 2019 / Published: 14 August 2019
(This article belongs to the Special Issue Bioactive Peptides—From Therapy to Nutrition)
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Abstract

Monoclonal antibodies with high affinity and specificity are essential for research and clinical purposes, yet remain difficult to produce. Agretope peptides that can potentiate antigen-specific antibody production have been reported recently. Here, we screened in silico for peptides with higher affinity against the agretope binding pocket in the MHC-II. The screening was based on the 3D crystal structure of a complex between MHC-II and a 14-mer peptide consisting of ovalbumin residues 323–339. Using this 14-mer peptide as template, we constructed a library of candidate peptides and screened for those that bound tightly to MHC-II. Peptide sequences that exhibited a higher binding affinity than the original ovalbumin peptide were identified. The peptide with the highest binding affinity was synthesized and its ability to boost antigen-specific antibody production in vivo and in vitro was assessed. In both cases, antigen-specific IgG antibody production was potentiated. Monoclonal antibodies were established by in vitro immunization using this peptide as immunostimulant, confirming the usefulness of such screened peptides for monoclonal antibody production. View Full-Text
Keywords: monoclonal antibody; MHC-II; agretope; in vitro immunization; in silico screening; IgG monoclonal antibody; MHC-II; agretope; in vitro immunization; in silico screening; IgG
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Hanyu, Y.; Komeiji, Y.; Kato, M. Potentiating Antigen-Specific Antibody Production with Peptides Obtained from In Silico Screening for High-Affinity against MHC-II. Molecules 2019, 24, 2949.

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