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Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC–ESI–TOF–MS

1
School of Pharmaceutical Sciences, Peking University, Beijing 100191, China
2
Kangmei Pharmaceutical Co.Ltd, Puning 515300, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Cédric Delattre
Molecules 2019, 24(14), 2526; https://doi.org/10.3390/molecules24142526
Received: 23 June 2019 / Revised: 8 July 2019 / Accepted: 8 July 2019 / Published: 10 July 2019
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Abstract

Polyporus umbellatus is a well-known and important medicinal fungus in Asia. Its polysaccharides possess interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects. A qualitative and quantitative method has been established for the analysis of 12 monosaccharides comprising polysaccharides of Polyporus umbellatus based on high-performance liquid chromatography coupled with electrospray ionization–ion trap–time of flight–mass spectrometry. The hydrolysis conditions of the polysaccharides were optimized by orthogonal design. The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C. The resulting monosaccharides derivatized with 1-phenyl-3-methyl-5-pyrazolone have been well separated and analyzed by the established method. Identification of the monosaccharides was carried out by analyzing the mass spectral behaviors and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides. The results showed that polysaccharides in Polyporus umbellatus were composed of mannose, glucosamine, rhamnose, ribose, lyxose, erythrose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and fucose. Quantitative recoveries of these monosaccharides in the samples were in the range of 96.10–103.70%. This method is simple, accurate, and sensitive for the identification and quantification of monosaccharides, and can be applied to the quality control of Polyporusumbellatus as a natural medicine. View Full-Text
Keywords: composition analysis; HPLC–MS; monosaccharide hydrolysis; Polyporus umbellatus; polysaccharide composition analysis; HPLC–MS; monosaccharide hydrolysis; Polyporus umbellatus; polysaccharide
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Guo, N.; Bai, Z.; Jia, W.; Sun, J.; Wang, W.; Chen, S.; Wang, H. Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC–ESI–TOF–MS. Molecules 2019, 24, 2526.

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