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Molecules 2018, 23(11), 2860; https://doi.org/10.3390/molecules23112860

Production of Selenomethionine-Enriched Bifidobacterium bifidum BGN4 via Sodium Selenite Biocatalysis

1
Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University, Seoul 08826, Korea
2
Korea Basic Science Institute, 145 Anamro, Sungbuk-Gu, Seoul 02841, Korea
3
Fermentation Science Program, School of Agriculture, College of Basic and Applied Sciences, Middle Tennessee State University, Murfreesboro, TN 37132, USA
4
Research Center, BIFIDO Co. Ltd., Hongcheon 25117, Korea
*
Authors to whom correspondence should be addressed.
Academic Editor: Jose M. Palomo
Received: 21 September 2018 / Revised: 26 October 2018 / Accepted: 1 November 2018 / Published: 2 November 2018
(This article belongs to the Special Issue Biomolecular Catalysts)
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Abstract

Selenium is a trace element essential for human health that has received considerable attention due to its nutritional value. Selenium’s bioactivity and toxicity are closely related to its chemical form, and several studies have suggested that the organic form of selenium (i.e., selenomethionine) is more bioavailable and less toxic than its inorganic form (i.e., sodium selenite). Probiotics, especially Bifidobacteriium and Lactobacillus spp., have received increasing attention in recent years, due to their intestinal microbial balancing effects and nutraceutical benefits. Recently, the bioconversion (a.k.a biotransformation) of various bioactive molecules (e.g., minerals, primary and secondary metabolites) using probiotics has been investigated to improve substrate biofunctional properties. However, there have been few reports of inorganic selenium conversion into its organic form using Bifidobacterium and Lactobacillus spp. Here we report that the biosynthesis of organic selenium was accomplished using the whole cell bioconversion of sodium selenite under controlled Bifidobacterium bifidum BGN4 culture conditions. The total amount of organic and inorganic selenium was quantified using an inductively coupled plasma-atomic emission spectrometer (ICP-AES). The selenium species were separated via anion-exchange chromatography and analyzed with inductively coupled plasma-mass spectrometry (ICP-MS). Our findings indicated that the maximum level of organic selenium was 207.5 µg/g in selenium-enriched B. bifidum BGN4. Selenomethionine was the main organic selenium in selenium-enriched B. bifidum BGN4 (169.6 µg/g). Considering that B. bifidum BGN4 is a commercial probiotic strain used in the functional food industry with clinically proven beneficial effects, selenium-enriched B. bifidum BGN4 has the potential to provide dual healthy functions as a daily supplement of selenium and regulator of intestinal bacteria. This is the first report on the production of organic selenium using B. bifidum spp. View Full-Text
Keywords: Bifidobacterium; functional foods; food additives; feed additives; probiotics; organic selenium; inorganic selenium; selenomethionine Bifidobacterium; functional foods; food additives; feed additives; probiotics; organic selenium; inorganic selenium; selenomethionine
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Jin, W.; Yoon, C.; Johnston, T.V.; Ku, S.; Ji, G.E. Production of Selenomethionine-Enriched Bifidobacterium bifidum BGN4 via Sodium Selenite Biocatalysis. Molecules 2018, 23, 2860.

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