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Molecules 2019, 24(4), 715; https://doi.org/10.3390/molecules24040715

Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica

1
Enzyme and Microbial Technology Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang Selangor 43400, Malaysia
2
National Antarctic Research Centre (NARC) B303, Block B, Level 3, IPS Building, University of Malaya, Kuala Lumpur 50603, Malaysia
3
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
4
British Antarctic Survey, NERC, High Cross, Madingley Road, Cambridge CB3 OET, UK
5
Department of Applied Sciences, Faculty of Health and Life Sciences, University of Northumbria at Newcastle, Newcastle-Upon-Tyne NE1 8ST, UK
6
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, Serdang Selangor 43400, Malaysia
*
Author to whom correspondence should be addressed.
Received: 23 November 2018 / Revised: 5 December 2018 / Accepted: 7 December 2018 / Published: 16 February 2019
(This article belongs to the Special Issue Biomolecular Catalysts)
Full-Text   |   PDF [1602 KB, uploaded 16 February 2019]   |  

Abstract

In recent years, studies on psychrophilic lipases have been an emerging area of research in the field of enzymology. This study focuses on bacterial strains isolated from anthropogenically-influenced soil samples collected around Signy Island Research Station (South Orkney Islands, maritime Antarctic). Limited information on lipase activities from bacteria isolated from Signy station is currently available. The presence of lipase genes was determined using real time quantification PCR (qPCR) in samples obtained from three different locations on Signy Island. Twenty strains from the location with highest lipase gene detection were screened for lipolytic activities at a temperature of 4 °C, and from this one strain was selected for further examination based on the highest enzymatic activities obtained. Analysis of 16S rRNA sequence data of this strain showed the highest level of sequence similarity (98%) to a Pseudomonas sp. strain also isolated from Antarctica. In order to increase lipase production of this psychrophilic strain, optimisation of different parameters of physical and nutritional factors were investigated. Optimal production was obtained at 10 °C and pH 7.0, at 150 rev/min shaking rate over 36 h incubation. View Full-Text
Keywords: Antarctica; cold adapted lipase; Pseudomonas sp. LSK25; lipase production Antarctica; cold adapted lipase; Pseudomonas sp. LSK25; lipase production
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Salwoom, L.; Raja Abd Rahman, R.N.Z.; Salleh, A.B.; Mohd. Shariff, F.; Convey, P.; Pearce, D.; Mohamad Ali, M.S. Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica. Molecules 2019, 24, 715.

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