Internalization of Titanium Dioxide Nanoparticles Is Cytotoxic for H9c2 Rat Cardiomyoblasts
AbstractTitanium dioxide nanoparticles (TiO2 NPs) are widely used in industry and daily life. TiO2 NPs can penetrate into the body, translocate from the lungs into the circulation and come into contact with cardiac cells. In this work, we evaluated the toxicity of TiO2 NPs on H9c2 rat cardiomyoblasts. Internalization of TiO2 NPs and their effect on cell proliferation, viability, oxidative stress and cell death were assessed, as well as cell cycle alterations. Cellular uptake of TiO2 NPs reduced metabolic activity and cell proliferation and increased oxidative stress by 19-fold measured as H2DCFDA oxidation. TiO2 NPs disrupted the plasmatic membrane integrity and decreased the mitochondrial membrane potential. These cytotoxic effects were related with changes in the distribution of cell cycle phases resulting in necrotic death and autophagy. These findings suggest that TiO2 NPs exposure represents a potential health risk, particularly in the development of cardiovascular diseases via oxidative stress and cell death. View Full-Text
Share & Cite This Article
Huerta-García, E.; Zepeda-Quiroz, I.; Sánchez-Barrera, H.; Colín-Val, Z.; Alfaro-Moreno, E.; Ramos-Godinez, M.P.; López-Marure, R. Internalization of Titanium Dioxide Nanoparticles Is Cytotoxic for H9c2 Rat Cardiomyoblasts. Molecules 2018, 23, 1955.
Huerta-García E, Zepeda-Quiroz I, Sánchez-Barrera H, Colín-Val Z, Alfaro-Moreno E, Ramos-Godinez MP, López-Marure R. Internalization of Titanium Dioxide Nanoparticles Is Cytotoxic for H9c2 Rat Cardiomyoblasts. Molecules. 2018; 23(8):1955.Chicago/Turabian Style
Huerta-García, Elizabeth; Zepeda-Quiroz, Iván; Sánchez-Barrera, Helen; Colín-Val, Zaira; Alfaro-Moreno, Ernesto; Ramos-Godinez, María P.; López-Marure, Rebeca. 2018. "Internalization of Titanium Dioxide Nanoparticles Is Cytotoxic for H9c2 Rat Cardiomyoblasts." Molecules 23, no. 8: 1955.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.