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Open AccessArticle

Rapid Characterization and Identification of Chemical Constituents in Gentiana radix before and after Wine-Processed by UHPLC-LTQ-Orbitrap MSn

Department of Medicine, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
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Author to whom correspondence should be addressed.
Molecules 2018, 23(12), 3222; https://doi.org/10.3390/molecules23123222
Received: 8 November 2018 / Revised: 2 December 2018 / Accepted: 3 December 2018 / Published: 6 December 2018
(This article belongs to the Special Issue Biological Sample Analysis by Liquid Chromatography)
Gentiana radix is used in traditional Chinese medicine and has functions of clearing heat and drying dampness, as well as purging liver and gallbladder fire. A highly sensitive and effective strategy for rapid screening and identification of target constituents has been developed by using ultra high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap) in crude and wine-processed Gentiana radix. Based on the accurate mass measurement (<5 ppm), retention times, and MS fragmentation ions, 52 constituents were unambiguously or tentatively characterized from Gentiana radix, including 21 iridoids, 11 flavonoids, 19 xanthones, and a triterpenoid. This study demonstrated that the established method could be a rapid, effective analytical tool for screening and characterization of compounds in the complex systems of Gentiana radix. By comparing the structure and peak areas of chemical constituents in crude and wine-processed Gentiana radix, we found that some compounds in crude and wine-processed Gentiana radix were significantly different. View Full-Text
Keywords: UHPLC-LTQ-Orbitrap; iridoids; flavonoids; xanthones; characteristic fragmentation pathways; wine-processing; Gentiana radix UHPLC-LTQ-Orbitrap; iridoids; flavonoids; xanthones; characteristic fragmentation pathways; wine-processing; Gentiana radix
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Lv, X.; Sun, J.-Z.; Xu, S.-Z.; Cai, Q.; Liu, Y.-Q. Rapid Characterization and Identification of Chemical Constituents in Gentiana radix before and after Wine-Processed by UHPLC-LTQ-Orbitrap MSn. Molecules 2018, 23, 3222.

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