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Molecules 2018, 23(12), 3321; https://doi.org/10.3390/molecules23123321

Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell Model

1,†
,
1,†
,
1
,
1
,
1,2
,
3,*
and
1,2,*
1
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China
2
Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210046, China
3
Wuhu Pure Sunshine Natural Medicine Company Limited, Wuhu 241000, China
*
Authors to whom correspondence should be addressed.
Jinlan Lu and Ling Liu contributed equally to the project and are considered as co-first authors.
Academic Editor: Thomas Efferth
Received: 31 October 2018 / Revised: 7 December 2018 / Accepted: 12 December 2018 / Published: 14 December 2018
(This article belongs to the Collection Herbal Medicine Research)
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Abstract

Qing’e Pills is a Chinese traditional herbal product, which is often used to strengthen muscles and bones in TCM (traditional Chinese Medicine) practice. Its two main component herbs, namely, Cortex Eucommiae and Fructus Psoraleae are both required to be salt-fried according to TCM theory. We have evaluated the effects of salt-frying treated herbs on Caco-2 cell uptake behavior for those active ingredients of Qing’e Pills. By investigating of various variables, including MTT, temperature, inhibitors, pH, salt concentration and herb processing methods, we tried to clarify whether the salt-processing on herbs was necessary or not. Results showed that, compared to other processing methods, the salt-frying process significantly (p < 0.01) enhanced the absorption of effective components of Qing’e Pills. The way that psoralen, isopsoralen, psoralenoside and geniposide acid entered Caco-2 cells at low concentrations was via passive diffusion. These components were not substrates of P-glycoprotein. It demonstrated that the salt-frying process not only enhanced the concentration of active components in herb extract, but also changed their absorption behaviors. Nevertheless, the mechanism of absorption behavior changing needs to be further investigated. View Full-Text
Keywords: Qing’e Pills; Caco-2 cell; absorption; salt-fried process Qing’e Pills; Caco-2 cell; absorption; salt-fried process
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Lu, J.; Liu, L.; Zhu, X.; Wu, L.; Chen, Z.; Xu, Z.; Li, W. Evaluation of the Absorption Behavior of Main Component Compounds of Salt-Fried Herb Ingredients in Qing’e Pills by Using Caco-2 Cell Model. Molecules 2018, 23, 3321.

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