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Molecules 2018, 23(10), 2550; https://doi.org/10.3390/molecules23102550

Composition Analysis by UPLC-PDA-ESI (−)-HRMS and Antioxidant Activity Using Saccharomyces cerevisiae Model of Herbal Teas and Green Teas from Hainan

Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan, 570228, China
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Received: 28 August 2018 / Revised: 30 September 2018 / Accepted: 3 October 2018 / Published: 6 October 2018
(This article belongs to the Special Issue Antioxidants Chemistry and Applications)
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Abstract

Different teas from everywhere are very useful and have been extensively studied. We studied the antioxidant activity of herbal teas and green teas from Hainan, Mallotus oblongifolius Muell. Arg. (MO), Ilex kudingcha C.J. Tseng (KD), Camellia sinensis var. assamica (J. W. Mast.) Kitam. Hainan Dayezhong (DY), and Camellia sinensis (L.) O. Ktze. (produced from Hainan Baisha (BS)). The total phenol content and total flavonoid content from water extracts, resin extracts and fractions of herbal teas and green teas were compared. Later, eight fractions of herbal teas and green teas were subjected to UPLC-PDA-ESI-(−)-HRMS. We determined 1-diphenyl -2-picryl-hydrazyl radical and hydroxyl free radical scavenging activity by electron paramagnetic resonance spectroscopy. We subjected Saccharomyces cerevisiae to hydrogen peroxide, stress and evaluated antioxidant activity of herbal teas and green teas in cellulo. The experiment identified more than 14 potential antioxidant compounds from herbal teas and green teas. The herbal teas and green teas had a clearance rate higher than ferulic acid at the same concentrations. MO best reduced intracellular oxidation levels and increased catalase, glutathione reductase activities, glutathione reduced and glutathione oxidized content. KD had the highest cell survival rate and reduced cell lipid peroxidation. DY best improved superoxide dismutase activity and BS was the most active in the halo test. Therefore, we concluded that MO had stronger antioxidant activity than other herbal teas and green teas from Hainan, especially, which reduce S. cerevisiae oxidative stress under H2O2 stress. View Full-Text
Keywords: UPLC-PDA-ESI-(−)-HRMS; EPR spectroscopy; Saccharomyces cerevisiae; antioxidant activity; H2O2 stress UPLC-PDA-ESI-(−)-HRMS; EPR spectroscopy; Saccharomyces cerevisiae; antioxidant activity; H2O2 stress
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Li, H.; Wang, L.; Luo, Y. Composition Analysis by UPLC-PDA-ESI (−)-HRMS and Antioxidant Activity Using Saccharomyces cerevisiae Model of Herbal Teas and Green Teas from Hainan. Molecules 2018, 23, 2550.

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