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A Comprehensive Study on the Biological Activity of Elderberry Extract and Cyanidin 3-O-Glucoside and Their Interactions with Membranes and Human Serum Albumin

1
Faculty of Life Sciences and Technology, Department of Physics and Biophysics, Wrocław University of Environmental and Life Sciences, C.K. Norwida 25, 50-375 Wrocław, Poland
2
Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria S.S. 554, Km 4.5, Monserrato, 09042 Cagliari, Italy
3
Faculty of Veterinary Medicine, Department of Pathology, Wrocław University of Environmental and Life Sciences, C.K. Norwida 31, 51-375 Wrocław, Poland
4
Faculty of Veterinary Medicine, Department of Biostructure and Animal Physiology, Wrocław University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wrocław, Poland
5
Faculty of Biotechnology and Food Science, Department of Fruit, Vegetable and Plant Nutraceutical Technology, Wrocław University of Environmental and Life Sciences, J. Chełmońskiego 37/41, 51-630 Wrocław, Poland
*
Author to whom correspondence should be addressed.
Academic Editors: María Pilar Almajano and Michael H. Gordon
Molecules 2018, 23(10), 2566; https://doi.org/10.3390/molecules23102566
Received: 10 September 2018 / Revised: 30 September 2018 / Accepted: 5 October 2018 / Published: 8 October 2018
(This article belongs to the Special Issue Antioxidants Chemistry and Applications)
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PDF [1510 KB, uploaded 8 October 2018]
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Abstract

In our research we used the extract from dietary supplement of elderberry (EE) and its dominant anthocyanin—cyanidin 3-O-glucoside (Cy 3-gluc). By interacting with a model membrane that reflects the main lipid composition of tumor membranes, the extract components, including Cy 3-gluc, caused an increase in packing order, mainly in the hydrophilic region of the membrane. It can thus be stated that EE caused a rigidifying effect, which is fundamental for understanding its anticancer and antioxidant activity. This study represents the first attempt to unravel the mechanism of interaction of elderberry extract with membranes. The results of the interaction with human serum albumin (HSA) proved that the studied substance quenches the fluorescence of HSA through a static mechanism in which the main interaction forces are Van der Waals and hydrogen bonding. The antioxidant activity of EE and Cy 3-gluc on liposomal membranes, antiradical properties and ability to inhibited the activity of the enzymes cyclooxygenase-1 and cyclooxygenase-2 were also demonstrated. Moreover, the anticancer activity of EE and Cy 3-gluc on human breast adenocarcinoma cell line were investigated. In addition, EE also exhibited the ability to form lipid aggregates in the form of liposomal capsules that can be applied as carriers of active biological substances, and the highest efficacy of EE encapsulation was obtained for multilayered liposome formulations. View Full-Text
Keywords: elderberry extract; cyanidin 3-O-glucoside; lipid membrane; albumin; cytotoxic properties; MCF-7 elderberry extract; cyanidin 3-O-glucoside; lipid membrane; albumin; cytotoxic properties; MCF-7
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Strugała, P.; Loi, S.; Bażanów, B.; Kuropka, P.; Kucharska, A.Z.; Włoch, A.; Gabrielska, J. A Comprehensive Study on the Biological Activity of Elderberry Extract and Cyanidin 3-O-Glucoside and Their Interactions with Membranes and Human Serum Albumin. Molecules 2018, 23, 2566.

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