Phenolics Isolated from Aframomum meleguta Enhance Proliferation and Ossification Markers in Bone Cells
AbstractOsteoporosis is a serious health problem characterized by decreased bone mineral density and deterioration of bone microarchitecture. Current antiosteoporotic agents exhibit a wide range of adverse effects; meanwhile, phytochemicals are effective and safer alternatives. In the current work, nine compounds belonging to hydroxyphenylalkane and diarylheptanoid groups were isolated from Aframomum meleguea seeds and identified as 6-gingerol (1), 6-paradol (2), 8-dehydrogingerdione (3), 8-gingerol (4), dihydro-6-paradol (5), dihydrogingerenone A (6), dihydrogingerenone C (7), 1,7-bis(3,4-dihydroxy-5-methoxyphenyl)heptane-3,5-diyl diacetate (8), and 1-(3,4-dihydroxy-5-methoxyphenyl)-7-(3,4-dihydroxyphenyl)heptane-3,5-diyl diacetate (9). The structures of isolated compounds were established by NMR and mass spectral data, in addition to referring to literature data. Exposure of MCF-7, MG-63, and SAOS-2 cells to subcytotoxic concentrations of the compounds under investigation resulted in accelerated proliferation. Among them, paradol was selected for further detailed biochemical analysis in SAOS-2 cells. DNA flowcytometric analysis of cell cycle distribution revealed that paradol did not induce any significant change in the proliferation index of SAOS-2 cells. Assessment of osteogenic gene expression revealed that paradol enhanced the expression of osteocyte and osteoblast-related genes and inhibited osteoclast and RUNX suppressor genes. Biochemically, paradol enhanced alkaline phosphatase activity and vitamin D content and decreased the osteoporotic marker acid phosphatase. In conclusion, paradol, which is a major constituents of A. melegueta seeds, exhibited potent proliferative and ossification characteristics in bone cells. View Full-Text
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Abdel-Naim, A.B.; Alghamdi, A.A.; Algandaby, M.M.; Al-Abbasi, F.A.; Al-Abd, A.M.; Abdallah, H.M.; El-Halawany, A.M.; Hattori, M. Phenolics Isolated from Aframomum meleguta Enhance Proliferation and Ossification Markers in Bone Cells. Molecules 2017, 22, 1467.
Abdel-Naim AB, Alghamdi AA, Algandaby MM, Al-Abbasi FA, Al-Abd AM, Abdallah HM, El-Halawany AM, Hattori M. Phenolics Isolated from Aframomum meleguta Enhance Proliferation and Ossification Markers in Bone Cells. Molecules. 2017; 22(9):1467.Chicago/Turabian Style
Abdel-Naim, Ashraf B.; Alghamdi, Abdullah A.; Algandaby, Mardi M.; Al-Abbasi, Fahad A.; Al-Abd, Ahmed M.; Abdallah, Hossam M.; El-Halawany, Ali M.; Hattori, Masao. 2017. "Phenolics Isolated from Aframomum meleguta Enhance Proliferation and Ossification Markers in Bone Cells." Molecules 22, no. 9: 1467.
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