Phenolic Glycosides from Capsella bursa-pastoris (L.) Medik and Their Anti-Inflammatory Activity
Abstract
:1. Introduction
2. Results and Discussion
2.1. Structure Elucidation
2.2. Anti-Inflammatory Activity
3. Materials and Methods
3.1. General Experimental Procedures
3.2. Plant Materials
3.3. Extraction and Isolation
3.4. Characterization
3.5. Enzymatic Hydrolysis
3.6. Measurement of Nitric Oxide Production and Cell Viability in LPS-Activated BV-2 Cells
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the authors. |
Position | δC, Type | δH (J in Hz) |
---|---|---|
1 | 135.7, C | |
2 | 111.3, CH | 7.05, d (1.7) |
3 | 147.8, C | |
4 | 147.5, C | |
5 | 118.1, CH | 7.15, d (8.2) |
6 | 119.5, CH | 6.95, dd (8.2, 1.7) |
7 | 88.6, CH | 5.58, d (5.91) |
8 | 55.7, CH | 3.48, m |
9 | 65.1, CH2 | |
a | 3.78, m | |
b | 3.85, m | |
1′ | 134.9, C | |
2′ | 114.7, CH | 6.78, s |
3′ | 147.9, C | |
4′ | 147.9, C | |
5′ | 145.5, C | |
6′ | 118.4, CH | 6.76, s |
7′ | 33.9, CH2 | |
a | 2.96, dd (13.5, 5.0) | |
b | 2.57, dd (13.3, 11.0) | |
8′ | 44.0, CH | 2.75, sep (6.5, 5.5) |
9′ | 73.5, CH2 | |
a | 3.78, m | |
b | 4.05, dd (8.3, 6.6) | |
1″ | 139.7, C | |
2″ | 111.5, CH | 7.01, d (1.7) |
3″ | 151.1, C | |
4″ | 147.5, C | |
5″ | 118.2, CH | 7.16, d (8.3) |
6″ | 119.7, CH | 6.90, dd (8.2, 1.7) |
7″ | 83.9, CH | 4.85, overlap |
8″ | 54.2, CH | 2.38, quin (6.9) |
9″ | 60.5, CH2 | |
a | 3.88, m | |
b | 3.68, dd (10.9, 6.7) | |
Glc-1′′′ | 103.1, CH | 4.91, d (7.3) |
2′′′ | 75.1, CH | 3.51, m |
3′′′ | 78.5, CH | 3.39, m |
4′′′ | 71.6, CH | 3.41, m |
5′′′ | 78.4, CH | 3.41, m |
6′′′ | 62.6, CH | 3.69, overlap |
OCH3 (3″) | 56.9 | 3.84, s |
OCH3 (3) | 56.8 | 3.88, s |
OCH3 (5′) | 56.8 | 3.87, s |
Compound | IC50 a (µM) | Cell Viability b (%) |
---|---|---|
1 | 75.13 | 103.04 ± 7.08 |
2 | 48.80 | 116.68 ± 3.69 |
3 | 30.70 | 109.17 ± 8.61 |
4 | 17.80 | 117.36 ± 10.23 |
5 | 31.14 | 116.09 ± 10.67 |
6 | 62.21 | 110.02 ± 9.52 |
7 | 27.91 | 119.20 ± 6.23 |
8 | 49.21 | 111.55 ± 10.40 |
c L-NMMA | 20.76 | 112.89 ± 4.90 |
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Cha, J.M.; Suh, W.S.; Lee, T.H.; Subedi, L.; Kim, S.Y.; Lee, K.R. Phenolic Glycosides from Capsella bursa-pastoris (L.) Medik and Their Anti-Inflammatory Activity. Molecules 2017, 22, 1023. https://doi.org/10.3390/molecules22061023
Cha JM, Suh WS, Lee TH, Subedi L, Kim SY, Lee KR. Phenolic Glycosides from Capsella bursa-pastoris (L.) Medik and Their Anti-Inflammatory Activity. Molecules. 2017; 22(6):1023. https://doi.org/10.3390/molecules22061023
Chicago/Turabian StyleCha, Joon Min, Won Se Suh, Tae Hyun Lee, Lalita Subedi, Sun Yeou Kim, and Kang Ro Lee. 2017. "Phenolic Glycosides from Capsella bursa-pastoris (L.) Medik and Their Anti-Inflammatory Activity" Molecules 22, no. 6: 1023. https://doi.org/10.3390/molecules22061023