Next Article in Journal
Clinical Signs, Staphylococcus and Atopic Eczema-Related Seromarkers
Next Article in Special Issue
Lycium barbarum L. Polysaccharide (LBP) Reduces Glucose Uptake via Down-Regulation of SGLT-1 in Caco2 Cell
Previous Article in Journal
Anti-Hyperglycemic Activity of Major Compounds from Calea ternifolia
Previous Article in Special Issue
β-Glucans: Relationships between Modification, Conformation and Functional Activities
 
Search for Articles:
Title / Keyword
Author / Affiliation
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
Journals
Molecules
Volume 22
Issue 2
10.3390/molecules22020287
molecules-logo
Submit to this Journal Review for this Journal Edit a Special Issue
► Article Menu

Article Menu

share announcement thumb_up
...
textsms
...
Open AccessArticle

Purification and Partial Structural Characterization of a Complement Fixating Polysaccharide from Rhizomes of Ligusticum chuanxiong

by
Yuan-Feng Zou
1,
Yu-Ping Fu
1,
Xing-Fu Chen
2,*,
Ingvild Austarheim
3,
Kari Tvete Inngjerdingen
3,
Chao Huang
1,
Lemlem Dugassa Eticha
3,
Xu Song
1,
Lixia Li
1,
Bin Feng
4,
Chang-Liang He
1,
Zhong-Qiong Yin
1 and
Berit Smestad Paulsen
3
1
Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China
2
Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, China
3
Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern 0316 Oslo, Norway
4
Animal Nutrition Institute, Sichuan Agricultural University, Wenjiang 611130, China
*
Author to whom correspondence should be addressed.
Academic Editor: Quan-Bin Han
Molecules 2017, 22(2), 287; https://doi.org/10.3390/molecules22020287
Received: 31 January 2017 / Revised: 31 January 2017 / Accepted: 8 February 2017 / Published: 14 February 2017
(This article belongs to the Special Issue Natural Polysaccharides)
View Full-Text Download PDF
Browse Figures

Abstract

Rhizome of Ligusticum chuanxiong is an effective medical plant, which has been extensively applied for centuries in migraine and cardiovascular diseases treatment in China. Polysaccharides from this plant have been shown to have interesting bioactivities, but previous studies have only been performed on the neutral polysaccharides. In this study, LCP-I-I, a pectic polysaccharide fraction, was obtained from the 100 °C water extracts of L. chuangxiong rhizomes and purified by diethylaminethyl (DEAE) sepharose anion exchange chromatography and gel filtration. Monosaccharide analysis and linkage determination in addition to Fourier transform infrared (FT-IR) spectrometer and Nuclear magnetic resonance (NMR) spectrum, indicated that LCP-I-I is a typical pectic polysaccharide, with homo-galacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and type II (AG-I/AG-II) side chains. LCP-I-I exhibited potent complement fixation activity, ICH50 of 26.3 ± 2.2 µg/mL, and thus has potential as a natural immunomodulator. View Full-Text
Keywords: Ligusticum chuanxiong; polysaccharides; purification; pectic polysaccharide; complement system Ligusticum chuanxiong; polysaccharides; purification; pectic polysaccharide; complement system
►▼ Show Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
SciFeed

Share and Cite

MDPI and ACS Style

Zou, Y.-F.; Fu, Y.-P.; Chen, X.-F.; Austarheim, I.; Inngjerdingen, K.T.; Huang, C.; Eticha, L.D.; Song, X.; Li, L.; Feng, B.; He, C.-L.; Yin, Z.-Q.; Paulsen, B.S. Purification and Partial Structural Characterization of a Complement Fixating Polysaccharide from Rhizomes of Ligusticum chuanxiong. Molecules 2017, 22, 287. https://doi.org/10.3390/molecules22020287

AMA Style

Zou Y-F, Fu Y-P, Chen X-F, Austarheim I, Inngjerdingen KT, Huang C, Eticha LD, Song X, Li L, Feng B, He C-L, Yin Z-Q, Paulsen BS. Purification and Partial Structural Characterization of a Complement Fixating Polysaccharide from Rhizomes of Ligusticum chuanxiong. Molecules. 2017; 22(2):287. https://doi.org/10.3390/molecules22020287

Chicago/Turabian Style

Zou, Yuan-Feng; Fu, Yu-Ping; Chen, Xing-Fu; Austarheim, Ingvild; Inngjerdingen, Kari T.; Huang, Chao; Eticha, Lemlem D.; Song, Xu; Li, Lixia; Feng, Bin; He, Chang-Liang; Yin, Zhong-Qiong; Paulsen, Berit S. 2017. "Purification and Partial Structural Characterization of a Complement Fixating Polysaccharide from Rhizomes of Ligusticum chuanxiong" Molecules 22, no. 2: 287. https://doi.org/10.3390/molecules22020287

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop