Next Article in Journal
Design, Synthesis and Bioactivities of Novel Isoxazole-Containing Pyrazole Oxime Derivatives
Next Article in Special Issue
A Lateral Flow Strip Based Aptasensor for Detection of Ochratoxin A in Corn Samples
Previous Article in Journal
Annotation of Peptide Structures Using SMILES and Other Chemical Codes–Practical Solutions
Previous Article in Special Issue
In Vitro Selection of DNA Aptamers that Binds Geniposide
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessReview

Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures

Centre for comparative Genomics, Murdoch University, Perth 6150, Australia
Perron Institute for Neurological and Translational Science, Perth 6005, Australia
School of Medicine, Deakin University, Waurn Ponds, Geelong 3216, Australia
Author to whom correspondence should be addressed.
Molecules 2017, 22(12), 2070;
Received: 7 November 2017 / Revised: 24 November 2017 / Accepted: 24 November 2017 / Published: 27 November 2017
(This article belongs to the Special Issue Nucleic Acid Aptamers)
PDF [1130 KB, uploaded 29 November 2017]


Systematic evolution of ligands by exponential enrichment (SELEX) is an established procedure for developing short single-stranded nucleic acid ligands called aptamers against a target of choice. This approach has also been used for developing aptamers specific to whole cells named Cell-SELEX. Aptamers selected by Cell-SELEX have the potential to act as cell specific therapeutics, cell specific markers or cell specific drug delivery and imaging agents. However, aptamer development is a laborious and time-consuming process which is often challenging due to the requirement of frequent optimization of various steps involved in Cell-SELEX procedures. This review provides an insight into various procedures for selection, aptamer enrichment, regeneration and aptamer-binding analysis, in addition to a very recent update on all aptamers selected by Cell-SELEX procedures. View Full-Text
Keywords: aptamers; Cell-SELEX; aptamer enrichment; ssDNA; binding assays aptamers; Cell-SELEX; aptamer enrichment; ssDNA; binding assays

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Rahimizadeh, K.; AlShamaileh, H.; Fratini, M.; Chakravarthy, M.; Stephen, M.; Shigdar, S.; Veedu, R.N. Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures. Molecules 2017, 22, 2070.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top