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Volume 22
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10.3390/molecules22111988
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Article

Lipid Peroxidation Process in Meat and Meat Products: A Comparison Study of Malondialdehyde Determination between Modified 2-Thiobarbituric Acid Spectrophotometric Method and Reverse-Phase High-Performance Liquid Chromatography

by
Anna Reitznerová
1,
Monika Šuleková
2,*,
Jozef Nagy
1,
Slavomír Marcinčák
1,
Boris Semjon
1,*,
Milan Čertík
3 and
Tatiana Klempová
3
1
Department of Food Hygiene and Technology, University of Veterinary Medicine and Pharmacy in Košice, 041 81 Košice, Slovakia
2
Department of Chemistry, Biochemistry and Biophysics, University of Veterinary Medicine and Pharmacy in Košice, 041 81 Košice, Slovakia
3
Institute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, 811 07 Bratislava, Slovakia
*
Authors to whom correspondence should be addressed.
Molecules 2017, 22(11), 1988; https://doi.org/10.3390/molecules22111988
Submission received: 28 September 2017 / Revised: 15 November 2017 / Accepted: 15 November 2017 / Published: 16 November 2017
(This article belongs to the Special Issue Selected Papers from the 46th EuroCongress on Drug Synthesis and Analysis (ECDSA-2017))
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Abstract

The aim of this work was to compare the methods of malondialdehyde detection, as the main secondary product of the lipid peroxidation process, in meat and meat products. Malondialdehyde measurements were performed by two modified methods, the 2-thiobarbituric acid spectrophotometric method and the reverse-phase high-performance liquid chromatography in raw, mechanically-deboned chicken meat and in manufactured frankfurters. The malondialdehyde concentrations measured by the 2-thiobarbituric acid spectrophotometric method were found to be overestimated by more than 25% in raw meat and more than 27% in frankfurters in comparison to the results of reverse-phase high-performance liquid chromatography (p < 0.05). The achieved results showed that the presented modified reverse-phase high-performance liquid chromatography method was more applicable and more accurate for the quantification of malondialdehyde in samples of meat and meat products.
Keywords: high-performance liquid chromatography; derivatization; thiobarbituric acid; spectrophotometry; malondialdehyde; lipid oxidation; meat; meat products high-performance liquid chromatography; derivatization; thiobarbituric acid; spectrophotometry; malondialdehyde; lipid oxidation; meat; meat products

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MDPI and ACS Style

Reitznerová, A.; Šuleková, M.; Nagy, J.; Marcinčák, S.; Semjon, B.; Čertík, M.; Klempová, T. Lipid Peroxidation Process in Meat and Meat Products: A Comparison Study of Malondialdehyde Determination between Modified 2-Thiobarbituric Acid Spectrophotometric Method and Reverse-Phase High-Performance Liquid Chromatography. Molecules 2017, 22, 1988. https://doi.org/10.3390/molecules22111988

AMA Style

Reitznerová A, Šuleková M, Nagy J, Marcinčák S, Semjon B, Čertík M, Klempová T. Lipid Peroxidation Process in Meat and Meat Products: A Comparison Study of Malondialdehyde Determination between Modified 2-Thiobarbituric Acid Spectrophotometric Method and Reverse-Phase High-Performance Liquid Chromatography. Molecules. 2017; 22(11):1988. https://doi.org/10.3390/molecules22111988

Chicago/Turabian Style

Reitznerová, Anna, Monika Šuleková, Jozef Nagy, Slavomír Marcinčák, Boris Semjon, Milan Čertík, and Tatiana Klempová. 2017. "Lipid Peroxidation Process in Meat and Meat Products: A Comparison Study of Malondialdehyde Determination between Modified 2-Thiobarbituric Acid Spectrophotometric Method and Reverse-Phase High-Performance Liquid Chromatography" Molecules 22, no. 11: 1988. https://doi.org/10.3390/molecules22111988

APA Style

Reitznerová, A., Šuleková, M., Nagy, J., Marcinčák, S., Semjon, B., Čertík, M., & Klempová, T. (2017). Lipid Peroxidation Process in Meat and Meat Products: A Comparison Study of Malondialdehyde Determination between Modified 2-Thiobarbituric Acid Spectrophotometric Method and Reverse-Phase High-Performance Liquid Chromatography. Molecules, 22(11), 1988. https://doi.org/10.3390/molecules22111988

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