Additives Enhancing the Catalytic Properties of Lipase from Burkholderia cepacia Immobilized on Mixed-Function-Grafted Mesoporous Silica Gel
Abstract
:1. Introduction
2. Results and Discussion
2.1. Immobilization of BcL onto Mixed-Function-Grafted Mesoporous Silica Gels
2.2. Effect of Additives on the Biocatalytic Properties of Adsorbed BcL
Additive | KR of rac-1a a | KR of rac-1b b | ||||||
---|---|---|---|---|---|---|---|---|
c (%) | ee(R)-2a (%) | E | Ub (µmol g−1 min−1) | c (%) | ee(R)-2b (%) | E | Ub (µmol g−1 min−1) | |
- | 2.3 | 98.1 | >100 | 1.6 | 2.1 | 76.6 | 7.7 | 2.7 |
Brij 30 | 5.1 | 98.9 | >100 | 3.5 | 4.2 | 74.3 | 7.0 | 5.5 |
Tween 80 | 9.9 | 99.2 | >200 | 6.7 | 8.8 | 74.9 | 7.5 | 11.5 |
PVA 4–88 | 26.1 | 99.5 | »200 | 17.9 | 27.5 | 75.0 | 9.2 | 35.9 |
PVA 18–88 | 22.5 | 99.4 | >200 | 15.4 | 21.8 | 75.2 | 8.6 | 28.4 |
PVA 13–23–88 | 25.8 | 99.3 | >200 | 17.6 | 27.2 | 76.4 | 9.8 | 35.4 |
PVA 72–98 | 14.8 | 98.9 | >200 | 10.1 | 14.0 | 74.5 | 7.7 | 18.2 |
PEG 8k | 24.6 | 99.5 | »200 | 16.8 | 26.7 | 74.0 | 8.7 | 34.8 |
PEG 20k | 30.0 | 99.0 | >200 | 20.5 | 34.7 | 74.6 | 10.1 | 45.1 |
Gum arabic | 21.8 | 99.3 | >200 | 14.8 | 19.9 | 74.6 | 8.2 | 25.8 |
Chitosan | 11.5 | 98.7 | >100 | 7.9 | 9.8 | 74.9 | 7.6 | 12.8 |
Lauric acid | 13.8 | 99.3 | >200 | 9.4 | 9.9 | 74.0 | 7.2 | 12.8 |
Oleic acid | 23.3 | 99.3 | >200 | 15.9 | 14.8 | 73.0 | 7.3 | 19.2 |
Hymono 9004 | 22.5 | 99.5 | »200 | 15.4 | 11.6 | 73.4 | 7.2 | 15.1 |
Trilaurin | 14.2 | 99.4 | >200 | 9.7 | 5.7 | 73.4 | 6.8 | 7.5 |
Triolein | 23.5 | 99.5 | »200 | 15.9 | 10.3 | 73.8 | 7.2 | 13.3 |
rac-1a | 10.8 | 99.0 | >200 | 7.3 | - | - | - | - |
rac-1b | - | - | - | - | 5.3 | 72.1 | 6.4 | 6.8 |
2.3. Effect of Additives on the Biocatalytic Properties of Covalently Immobilized BcL
KR of rac-1a a | KR of rac-1b b | |||||
---|---|---|---|---|---|---|
Additive | c (%) | ee(R)-2a (%) | E | c (%) | ee(R)-2b (%) | E |
- | 0.3 | 89.8 | 18.6 | 0.5 | 70.4 | 5.8 |
PEG 4k | 1.2 | 97.9 | 94.8 | 1.3 | 71.4 | 6.1 |
PEG 20k | 11.8 | 99.5 | »200 | 22.3 | 73.3 | 8.2 |
Tween 80 | 7.3 | 99.6 | »200 | 9.4 | 71.6 | 6.5 |
PVA 18–88 | 14.0 | 99.8 | »200 | 26.4 | 75.2 | 9.2 |
Gum arabic | 13.5 | 99.8 | »200 | 26.1 | 75.5 | 9.3 |
Lauric acid | 4.0 | 99.4 | >200 | 8.5 | 74.7 | 7.4 |
Oleic acid | 3.4 | 99.4 | >200 | 9.9 | 74.8 | 7.5 |
Triolein | 8.7 | 99.7 | »200 | 13.0 | 73.3 | 7.2 |
2.4. Thermal Stability of BcLs Adsorbed onto Mixed-Function-Grafted Mesoporous Silica Gel
2.5. Recyclability of Adsorbed BcL Biocatalysts
2.6. Continuous-Flow Kinetic Resolutions of rac-1a with BcLs Adsorbed with PVA 18-88 and PEG 20k
3. Experimental Section
3.1. Analytical Methods
3.2. Materials
3.3. BcL Immobilization by Adsorption on Mesoporous Silica gel with Mixed Grafting
3.4. BcL Immobilization by Adsorption Followed by Cross-Linking on Mesoporous Silica Gel with Mixed Grafting
3.5. Enantiomer Selective Acetylation of Racemic 1-Phenylethanol rac-1a and 1-(Thiophen-2-yl)ethan-1-ol rac-1b
3.6. Thermal Stability of Immobilized BcL Biocatalysts
3.7. Recycling the Immobilized BcL Biocatalysts
3.8. Kinetic Resolution of 1-Phenylethanol rac-1a in Adsorbed BcL-Filled Continuous-Flow Bioreactor
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Abaházi, E.; Boros, Z.; Poppe, L. Additives Enhancing the Catalytic Properties of Lipase from Burkholderia cepacia Immobilized on Mixed-Function-Grafted Mesoporous Silica Gel. Molecules 2014, 19, 9818-9837. https://doi.org/10.3390/molecules19079818
Abaházi E, Boros Z, Poppe L. Additives Enhancing the Catalytic Properties of Lipase from Burkholderia cepacia Immobilized on Mixed-Function-Grafted Mesoporous Silica Gel. Molecules. 2014; 19(7):9818-9837. https://doi.org/10.3390/molecules19079818
Chicago/Turabian StyleAbaházi, Emese, Zoltán Boros, and László Poppe. 2014. "Additives Enhancing the Catalytic Properties of Lipase from Burkholderia cepacia Immobilized on Mixed-Function-Grafted Mesoporous Silica Gel" Molecules 19, no. 7: 9818-9837. https://doi.org/10.3390/molecules19079818