Next Article in Journal
Cytoprotective Effects of β-Melanocortin in the Rat Gastrointestinal Tract
Previous Article in Journal
Matsuno, A., et al., Molecular Morphology of Pituitary Cells, from Conventional Immunohistochemistry to Fluorescence Imaging. Molecules 2011, 16, 3618-3635
Article Menu

Export Article

Open AccessArticle
Molecules 2012, 17(10), 11669-11679;

Study of Active Ingredients in Black Soybean Sprouts and Their Safety in Cosmetic Use

School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, Jiangsu, China
School of Chemical Engineering, Hebei United University, Tangshan 063009, Hebei, China
Shanghai Jahwa United Co. Ltd, Shanghai 200082, China
School of Science, Beijing Technology and Business University, Beijing 100048, China
Author to whom correspondence should be addressed.
Received: 10 July 2012 / Revised: 13 September 2012 / Accepted: 19 September 2012 / Published: 1 October 2012
Full-Text   |   PDF [335 KB, uploaded 18 June 2014]   |  


Active ingredients in different lengths of black soybean sprouts were extracted with water. Concentrations of the main proteins and polysaccharides were determined by the Forint phenol assay and phenol-sulfuric acid assay, respectively. Anti-oxidizing capacities of the extracts were measured in vitro using the DPPH scavenging test and whitening capacity was measured in vitro using the tyrosinase inhibition test. The effects of the bean sprout extracts on human skin fibroblasts damnified by H2O2 were studied using an MTT colorimetric assay. The safety of the extracts was determined using the red blood cell (RBC) test, chick chorioallantoic membrane (CAM) assay and human patch test. Results show that DPPH radical scavenging rates at different shoot lengths were all greater than 95%, while the tyrosinase inhibition capacity of the extracts reached 98%. Hemolysis rate in all extracts were lower than 10%, below the 20% regulatory limit for the RBC test. No signs of allergic reactions were observed in the human patch tests. The optimum extract was obtained from bean sprouts grown to 0.5 cm. Extracts of black bean sprouts are safe and can be used as additives in anti-aging and whitening cosmetic products. View Full-Text
Keywords: additives; cosmetics; black soybean sprouts; anti-oxidation; whitening; safety additives; cosmetics; black soybean sprouts; anti-oxidation; whitening; safety

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Lai, J.; Xin, C.; Zhao, Y.; Feng, B.; He, C.; Dong, Y.; Fang, Y.; Wei, S. Study of Active Ingredients in Black Soybean Sprouts and Their Safety in Cosmetic Use. Molecules 2012, 17, 11669-11679.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top