Topic Editors

Department of Molecular Medicine and Medical Biotechnology, School of Medicine, University of Naples Federico II, 80131 Naples, Italy
Department of Biomedical, Surgical and Dental Sciences-One Health Unit, School of Medicine, University of Milan, Via Pascal 36, 20133 Milan, Italy

Label-Free Proteome Profiling

Abstract submission deadline
closed (15 December 2022)
Manuscript submission deadline
closed (15 March 2023)
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4345

Topic Information

Dear Colleagues,

The accurate determination of quantitative changes describing biological systems has attracted considerable interest from the scientific community. Biological processes are regulated through the abundance of proteins. In addition, the relative abundance of each protein in a complex system may influence the modulation of its activity and function. Over the past decade, mass-spectrometry-based proteomics has become an indispensable tool in deciphering the exact abundance of each protein in composite mixtures. Indeed, the fast development of mass-spectrometry-based proteomic technologies and the accessibility of powerful data analysis tools have increasingly boosted the transition of proteomic analysis from qualitative to quantitative, with a strong impact on the biological interpretation of protein functions. The described technological improvement, such as the advent of new high-resolution instruments, has allowed us to overcome classical, label-based quantitative approaches and use proteomics to move toward label-free techniques.

This Topical Collection invites original and interesting contributions of quantitative proteomic studies and aims to describe the translational applicability of label-free proteomic profiling in a wide range of scientific fields. However, the Topical Collection is also open to discussions about the methodological progress linked to quantitative proteomics to facilitate the sharing of experimental and technical details in terms of methodological conditions and bioinformatic analysis.

Dr. Marianna Caterino
Dr. Alessio Soggiu
Topic Editors

Keywords

  • label-free quantitation
  • mass spectrometry-based quantitative analysis
  • biofluids and tissue quantitative proteomics
  • proteome dynamics
  • protein-protein interactions in macromolecular complexes

Participating Journals

Journal Name Impact Factor CiteScore Launched Year First Decision (median) APC
Molecules
molecules
4.2 7.4 1996 15.1 Days CHF 2700
Proteomes
proteomes
4.0 6.5 2013 34.2 Days CHF 1800

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Published Papers (1 paper)

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14 pages, 2053 KiB  
Article
Insight of the Functional and Biological Activities of Coconut (Cocos nucifera L.) Protein by Proteomics Analysis and Protein-Based Bioinformatics
by Jingrong Ma, Chuang Pan, Haiming Chen, Weijun Chen, Wenxue Chen, Ming Zhang and Qiuping Zhong
Molecules 2022, 27(9), 2987; https://doi.org/10.3390/molecules27092987 - 6 May 2022
Cited by 9 | Viewed by 3131
Abstract
Coconut (Cocos nucifera L.) is one of the most critical economic crops in the tropics and sub-tropics. Although coconut protein has attracted more and more attention due to its nutritional potential, the lack of proteomic information has limited its practical application. The [...] Read more.
Coconut (Cocos nucifera L.) is one of the most critical economic crops in the tropics and sub-tropics. Although coconut protein has attracted more and more attention due to its nutritional potential, the lack of proteomic information has limited its practical application. The present study aimed to investigate the coconut meat proteome by shotgun proteomics and protein-based bioinformatic analysis. A grand total of 1686 proteins were identified by searching the National Center for Biotechnology Information (NCBI) protein database and self-constructed C. nucifera transcriptome repository. Among them, 17 and 9 proteins were identified as antioxidant proteins and globulins, respectively. Network analysis of the globulins referred to the sub-works of Cupin and Oleosin, and the antioxidant proteins were related to the sub-networks of glutathione metabolism and peroxisome. The bioactive peptides acquired by in-silico digestion of the targeted proteins have the potential to be applied as antioxidants and emulsifiers for both healthcare and food stabilization. Full article
(This article belongs to the Topic Label-Free Proteome Profiling)
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