Search Results (1,383)

This study presents the results of long-term field experiments investigating the effects of subsurface heating and irrigation with discharged warm water (28–35 °C) on the hydrothermal, redox, biological, and salt regimes of Haplic Chernozem (Loamic, Endocalcaric) in the central Forest-Steppe zone of Ukraine. The experiments were conducted under contrasting hydro-meteorological conditions, allowing assessment of thermo-reclamation practices across a wide range of temperature and soil moisture regimes. Subsurface heating increased soil temperature by 7.3–11.1 °C at the depth of heating pipe installation, while the thermal effect gradually decreased with increasing distance from the heat source. Combined heating and irrigation created a more uniform temperature distribution within the root zone, reduced the depth and duration of soil freezing, and improved hydrothermal conditions throughout the growing season. The studied soils maintained predominantly oxidative conditions under all treatments. However, the combined application of heating and irrigation promoted a more homogeneous distribution and seasonal stabilization of soil redox potential throughout the profile. Soil heating also enhanced microbiological activity, thereby increasing cellulolytic activity, particularly during cold and dry periods when soil temperature and moisture limited microbial processes. Irrigation with slightly mineralized warm wastewater did not cause significant overall soil salinization but resulted in the redistribution of calcium and sodium within the soil profile. Subsurface heating intensified the seasonal dynamics of readily soluble salts, promoting their temporary accumulation near the heating pipes, whereas combined heating and irrigation facilitated subsequent leaching of excess salts into deeper horizons. The integrated application of subsurface heating and irrigation produced the highest and most stable perennial grass productivity, increasing biomass yield by 87–163% compared with the control, irrespective of meteorological conditions. These findings demonstrate that the integrated use of industrial waste heat for subsurface heating combined with irrigation represents a promising and environmentally sustainable thermo-reclamation technology capable of improving soil functioning and agricultural productivity, provided that long-term monitoring of soil water–salt regimes is maintained. Full article

This study evaluated oxidative damage, antioxidant-related, and nitric oxide (NO)-related chemical markers in commercial bee pollen using two extraction systems. For this purpose, a total of 30 bee pollen samples from different brands and/or manufacturers were examined; each sample was subjected to aqueous extraction and ethanol/water (80:20, v/v). The levels of reduced glutathione (GSH), malondialdehyde (MDA), and NO metabolites, comprising nitrite and nitrate and expressed as total NOx, were determined in pollen extracts using spectrophotometric methods. The data were analyzed using descriptive statistics, group comparisons, and Spearman correlation analysis. The findings showed that ethanol/water (80:20, v/v) extraction provided significantly higher measured GSH equivalents and total NOx levels than aqueous extraction. Mean GSH equivalents were 79.12 ± 25.54 µmol/g in the aqueous extracts and 189.77 ± 45.09 µmol/g in the ethanol/water extracts, and the difference was found to be statistically significant (p < 0.001). NO metabolite levels were determined as 5.97 ± 8.34 and 366.70 ± 85.10 µmol/g, respectively, and the difference between the extraction methods was found to be statistically significant (p < 0.001). In contrast, MDA levels did not show a significant difference between the two extraction systems (p = 0.391). A significant positive correlation was observed between GSH and NO metabolites in the combined dataset (r = 0.660, p < 0.001). The findings suggest that 80% ethanolic bee pollen extract may represent a promising source of NO metabolites for future investigation as a natural alternative to synthetic sodium nitrite in processed meat products. However, this hypothesis requires validation through microbiological, technological, sensory, and shelf-life studies. Full article

Cannabidiol (CBD)-infused bottled water has recently become one of the new types of functional beverages in the expanding cannabinoid and nutraceutical market. This paper presents preliminary experimental observations combined with a literature-based analysis to investigate quality and stability parameters of CBD-infused bottled water. Physicochemical characterization, microbial quality analysis and stability monitoring of CBD under storage conditions were included in the experimental work. The results indicated that the formulation maintained the properties of its initial dispersion during the first period of storage. An increase in transparency and a decrease in CBD concentration were noted. Microbial counts increased during storage, suggesting that microbiological stability is limited by time under the tested conditions. Reviewing the literature underlines the significance of cannabinoid stability in aqueous beverages aided by nanoemulsion delivery systems, packaging materials and controlled storage environments. The results show that CBD can be infused into bottled water but challenges regarding formulation and storage exist. The research was not conducted to address long shelf-life, commercial scalability or compliance with regulations but to provide primary information on quality and stability factors influencing CBD beverages. More research using standardized procedures is required to understand safety, efficacy and stability over time. Full article

Pork jerky is vulnerable to quality deterioration during distribution, particularly under hot and humid conditions. This study investigated the deterioration pattern and associated mechanisms for vacuum-packaged pork jerky sealed in aluminum foil pouches during accelerated high-temperature and high-humidity storage (65 °C and relative humidity of 85%). The sensory quality, physicochemical properties, oxidation-related changes, non-enzymatic browning, and protein digestibility were evaluated, and multivariate analyses were performed to characterize the deterioration process. Sensory acceptability declined markedly, and the rejection rate reached 50% on day 14, indicating the shelf-life endpoint under the test conditions. Deterioration in quality was mainly characterized by color darkening, texture hardening, water redistribution, and microstructural disruption. Lipid oxidation, protein oxidation, myoglobin oxidation, and non-enzymatic browning progressively intensified during storage and were associated with the observed color deterioration and sensory decline. Microbiological indicators remained below the detection limit throughout storage, suggesting that quality deterioration was primarily associated with chemical rather than microbial changes. Multivariate analyses further suggested that lipid oxidation, protein oxidation, myoglobin oxidation, and non-enzymatic browning were key processes potentially associated with quality loss. These findings provide a basis for shelf-life evaluation and quality control for pork jerky under extreme storage conditions. Full article

Background/Objectives: This exploratory study aimed to characterize the microbiological contamination of dental unit waterlines (DUWLs) and to conduct a preliminary evaluation of a 0.5% sodium hypochlorite shock treatment. Methods: Water samples from five dental units in a single teaching institution were collected from the air/water syringe and high-speed handpiece lines at four time points: baseline, immediately after a shock treatment, and 7 and 14 days post-treatment. The water supply source was also analyzed. The intervention consisted of a 15-min exposure to 0.5% sodium hypochlorite. Bacterial and fungal contamination was assessed using validated semi-quantitative microbial samplers, and key bacterial isolates were identified. Results: Baseline assessments revealed substantial bacterial contamination in most units (≥301 CFU/mL), despite minimal contamination in the water supply source (<10 CFU/mL). An immediate reduction in microbial counts was observed following the shock treatment for both bacteria and fungi counts; however, resilient species such as P. aeruginosa and the spore-forming B. cereus persisted, while K. pneumoniae was no longer detected. Bacterial levels increased again within 7 days in several units, while fungal contamination remained low at the follow-up. At 14 days, bacterial results were variable and inconsistent. In contrast, fungal contamination remained suppressed in most units throughout the follow-up period. Conclusions: The sodium hypochlorite shock treatment produced an immediate decrease in microbial load in DUWLs, but the findings suggest that its sustained effect on bacterial control may be limited. Given the small sample size and single-center design, these results should be interpreted cautiously. The study demonstrates the feasibility of implementing a structured DUWL monitoring and maintenance program using a simple, low-cost sodium hypochlorite protocol, providing a practical model for improving water quality and patient safety in dental clinics. Full article

This study was designed to demonstrate the effect of Pinot Noir grape pomace extract (GPE) on the quality and shelf-life of frankfurter sausages during 35 days of storage at 4 °C. Due the high polyphenolic compounds of GPE, there is an increasing interest in potentially replacing the artificial additives with natural alternatives generated from waste. The GPE was prepared using ultrasound-assisted extraction (UAE). Following ultrasound-assisted extraction, the extracts were filtered through filter paper to remove solid residues. The meat batter was divided into four groups: control (NFC) and three experimental batches with 1%, 2%, and 3% Pinot Noir grape pomace extract (FE1, FE2, FE3). Results showed that increasing GPE significantly raised water and protein levels while reducing fat content and water activity (p < 0.05). Except for a decrease in the b* color value, GPE did not notably affect pH, collagen, or L* and a* color parameters. Although the chemical parameters changed, the collagen content remained unchanged, indicating that GPE does not significantly affect connective tissues. The best effect was shown in the sample FE3, which had the highest GPE addition and exhibited the highest protein content (16.46%) and moisture content (64.99%), as well as the lowest water activity (0.93). Microbiologically, GPE reduced aerobic mesophilic bacteria, with all samples remaining safe for up to 28 days. These findings confirm GPE as a viable ingredient for producing safe, high-quality frankfurter sausages. Full article

This study provides a preliminary baseline characterization of macroinvertebrate assemblages colonizing floating islands installed in two NE Portuguese river reaches with contrasting anthropogenic and hydromorphological conditions: the urban, weir-influenced Fervença River and the more rural, reservoir-like Côa reach within a former dam-work area. A single campaign in February 2025 combined water characterization and macroinvertebrate sampling on islands and adjacent margins. Fervença showed higher mineralization and microbiological contamination, but a more varied assemblage dominated by tolerant taxa. Côa showed better water quality but strong Astacidae dominance. Results indicate colonization only; robust replicated monitoring is needed before inferring ecological improvement. Full article

Catfish (Clarias macrocephalus × C. gariepinus) is economically important but perishable, and hot-air drying yields shelf-stable catfish powder (CFP); however, its quality response to high-temperature drying (>100 °C) remains poorly understood. The present study aimed to examine the effects of five drying temperatures (60–180 °C) on the physicochemical, nutritional, functional, structural, volatile, oxidative, and microbiological properties of CFP. Drying exerted parameter-specific effects. Moisture and water activity fell from 6.24% to 4.75% and 0.42 to 0.23, with microbial counts reduced at ≥120 °C. Browning raised lightness, redness, and yellowness from 49.53 to 57.64, 1.94 to 2.97, and 13.95 to 18.14, respectively. Protein concentrated from 57.63% to 62.24%, whereas oxidation lowered fat from 22.49% to 17.17%. Minerals rose marginally, although iron and zinc declined. Amino acids were largely preserved, though methionine (2.71 to 2.21 g/100 g protein) and cysteine declined and glycine and proline increased. DHA declined most, from 4.61% to 3.20%, followed by EPA and AA. Water holding, oil holding, wettability, dispersibility, and colloidal stability decreased, whereas emulsion stability decreased from 57.14% at 60 °C to 42.10% at 180 °C. Zeta potential became less negative (−35.82 to −20.74 mV), with larger particle size and polydispersity. Among 72 volatiles across 11 classes, 60–90 °C favored fishy aldehydes and 2-pentyl furan and ≥150 °C generated Maillard and sulfur compounds, whereas 120 °C produced the most balanced volatile profile, with lower relative abundance of fishy aldehydes than at 60–90 °C, consistent with the intermediate TBARS values and comparatively better retention of unsaturated fatty acids at this temperature, and limited accumulation of Maillard-derived and sulfur compounds relative to 150–180 °C. FTIR, XRD, TGA, and SEM confirmed preserved structure. Overall, the results showed that 120 °C provided the best balance of dehydration, nutrient and functional retention, flavor, and microbial safety. Full article

This study developed candied pumpkin using Jerusalem artichoke syrup as an alternative to sucrose and evaluated its physicochemical properties, microstructure, sensory quality, and storage stability. Five formulations with Jerusalem artichoke syrup-to-pumpkin ratios of 1.25:1.00–1.30:1.00 were compared with a sucrose-based control. Syrup-based samples contained more protein (0.96–0.99 g/100 g) and fewer carbohydrates (21.25–23.35 g/100 g) than the control (0.40 and 40.40 g/100 g, respectively). Their caloric values ranged from 90.37 to 99.34 kcal/100 g, compared with 163.20 kcal/100 g in the control. Initial hardness was lower in syrup-based samples (30–40 kPa) than in the control (45 kPa), indicating a less rigid texture. Microstructural analysis showed preserved fibrous organization and a more uniform matrix, particularly in Sample 3 (1.27:1.00). This formulation also showed favorable sensory characteristics and higher contents of vitamin C, pantothenic acid, niacin, phosphorus, magnesium, sodium, and iron. During storage, moisture content decreased and water activity increased gradually but remained below 0.60. Microbial counts remained within permissible limits for 9 months, with no detectable coliforms or pathogenic microorganisms. Therefore, Jerusalem artichoke syrup may be considered a promising sucrose substitute for producing candied pumpkin with improved nutritional value and acceptable storage stability. Full article

The present study investigates the development of corn starch-based films incorporating red cabbage extract (RCE) as natural colorimetric freshness indicators for minced chicken. This work addresses the need for safe, natural, and non-contact freshness indicators by integrating anthocyanin-rich RCE into corn starch films and evaluating their response to spoilage-related pH changes in poultry. Although anthocyanin-based films have been previously reported, their application as non-contact indicators for minced chicken—validated simultaneously through microbiological, physicochemical, and volatile compound analyses—has received limited attention. Corn starch films (CSFs), without and with RCE at different concentrations, were prepared and evaluated for thickness, mechanical properties, moisture content, and water solubility. The colorimetric response of CSF + RCE specimens to pH changes associated with meat spoilage was evaluated under refrigerated storage. The spoilage of minced chicken was independently monitored through microbiological analyses [total viable count (TVC), Pseudomonas, Brochothrix thermosphacta, Enterobacteriaceae, lactic acid bacteria (LAB), yeasts and molds] and physicochemical measurements [pH, total volatile basic nitrogen (TVB-N) and volatile compound profile]. Both microbiological and physicochemical indicators confirmed the onset of spoilage between the 5th and 7th days of storage, with unacceptable pH, TVB-N, and microbial levels being observed on the 7th day. The films exhibited visible though limited color changes in response to spoilage-related pH shifts. While the narrow pH range of poultry spoilage constrained the intensity of visual differences, the results demonstrate the potential of RCE-based films to be considered preliminary non-contact indicators and to support more sustainable food systems by enabling real-time freshness monitoring and potentially reducing unnecessary food waste. This study also highlights the need for future work incorporating objective colorimetric measurements (e.g., CIELab) and structural characterization to optimize indicator sensitivity. Full article

Aquaponics is an integrated and resource-efficient production system that combines aquaculture and hydroponics in a closed-loop environment with reduced water consumption and nutrient losses. The present study evaluated the nutritional composition, mineral profile, microbiological quality, and antioxidant-related phytochemical characteristics of red amaranth (Amaranthus cruentus L.) cultivated in a recirculating aquaponic system under controlled environmental conditions. Leaf biomass was analyzed for proximate composition, macro- and micronutrient content, total phenolic and flavonoid compounds, betalains, chlorophyll pigments, and antioxidant activity using standard analytical and spectrophotometric methods. The results demonstrated high crude protein content and substantial accumulation of essential minerals, particularly calcium, potassium, and magnesium. The analyzed biomass also exhibited elevated levels of phenolic compounds, flavonoids, betalains, and chlorophyll pigments associated with considerable antioxidant potential. The pigment profile suggested good physiological adaptation of plants to aquaponic cultivation conditions. In addition, microbiological analysis confirmed acceptable hygienic quality and safety of the harvested plant material. Overall, the findings indicate that red amaranth can be successfully cultivated in aquaponic systems while maintaining high nutritional value and functional food potential. The study highlights aquaponic cultivation as a sustainable approach to producing nutrient-dense leafy vegetables within environmentally responsible agricultural systems. Full article

The use of non-conventional systems for domestic wastewater management has gained attention in rural areas of the Global South, where centralised infrastructure is often limited. This study presents the design, construction, and pilot-scale evaluation of a solar still unit operated under passive and photovoltaic-assisted active modes as a separation and polishing component for domestic wastewater from a rural site in Barrancabermeja, Colombia. Performance was assessed through physicochemical and microbiological characterisation of influent wastewater and treated condensate, together with hourly monitoring of distillate production, water temperature, glass-cover temperature, and ambient conditions. Under passive operation, a theoretical distillation model was applied, empirically adjusted, and evaluated using MAE, RMSE, MAPE, and R². Under the tested conditions, indicative within-mode reductions reached 80.9% and 89.3% for chemical oxygen demand (COD), 95.6% and 93.8% for biochemical oxygen demand (BOD₅), and 94.0% and 94.4% for total suspended solids (TSS) under passive and active modes, respectively. Microbial indicators showed minimum estimated reductions above 99.9%, with faecal coliforms reduced to very low levels in passive mode and not detected in the analysed active-mode condensate sample. Maximum daily condensate production reached 1.445 L m⁻² day⁻¹ in passive mode and 2.262 L m⁻² day⁻¹ in active mode, confirming the low-flow nature of the unit. Approximately 50% of daily production occurred between 12:00 and 15:00 h. The model reproduced the main diurnal production pattern, although empirical correction was required. Overall, the unit may improve condensate quality under pilot-scale conditions and shows potential as a polishing component within decentralised, low-flow treatment trains. Full article

Microbiological contamination of surface water represents a critical public health concern, while conventional disinfectants face limitations such as the generation of toxic by-products and the emergence of microbial resistance. In this study, the application of an ethanolic peel extract of Citrus paradisi (grapefruit), obtained by sonication at 40 kHz for 90 min at 55 °C using a 1:4 (w/v) solvent-to-solid ratio, was evaluated as a natural alternative for bacterial reduction in contaminated waters. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extract were first determined against Staphylococcus aureus and Escherichia coli. The extract was then applied to samples of slightly contaminated surface water with bacterial loads between 10³–10⁴ CFU/mL and turbidity of 150 NTU, as well as to highly contaminated surface water with bacterial loads ≥10⁵ CFU/mL and turbidity of 250 NTU. Bacterial removal was assessed at 6, 12, and 24 h. FTIR and UV-Vis characterization of the extract confirmed the presence of flavonoids (naringin), terpenes (limonene), and phenolic compounds. Results showed MIC/MBC values of 2.5/5.0 mg/mL for S. aureus and 5.0/10.0 mg/mL for E. coli. In slightly contaminated water, the extract at 5.0 mg/mL achieved complete (100%) removal of both microorganisms after 12 h, whereas in highly contaminated water, removals ranged from 80–90% for Staphylococcus spp. and E. coli. Statistical analysis (ANOVA, Bonferroni) demonstrated significant differences (p < 0.05) between the extract and ethanol. These findings indicate that Citrus paradisi extract constitutes an effective, sustainable, and low-cost natural alternative for bacterial reduction in surface waters, contributing to the valorization of agro-industrial residues and to the achievement of Sustainable Development Goal (SDG) 6. Full article

Ornamental aquarium keeping collectively involves millions of freshwater, marine, and reef systems in which fish, corals, invertebrates, biofilters, microbial communities, and human husbandry practices are closely interconnected. In these domestic aquatic animal systems, preventive and curative treatments may include antimicrobials, antiparasitics, antiseptics, oxidizing agents, copper-based products, dips, and commercial formulations targeting microbial proliferations or visible system deterioration. Many interventions occur without veterinary diagnosis, microbiological confirmation, standardized dosing, active-ingredient transparency, or post-treatment monitoring. This raises concerns for aquatic animal health and welfare, as whole-system treatments may affect not only the intended pathogen or pest but also non-target organisms, biofilter communities, animal-associated microbiota, and water quality stability. Digital communities and online platforms can rapidly circulate empirical treatment protocols, although they may also provide opportunities for stewardship education and improved husbandry guidance. Current evidence does not support interpreting ornamental aquaria as major independent drivers of antimicrobial resistance. The more defensible concern is stewardship: biologically active compounds may be used repeatedly and empirically in animal systems without diagnosis, professional guidance, or systematic monitoring. This Perspective argues that ornamental aquaria should be recognized as an overlooked interface between aquatic animal health, welfare, antimicrobial stewardship, and One Health. It proposes a research and communication agenda focused on treatment transparency, diagnosis, prevention, biofilter protection, and responsible care practices. Full article

The aim of the study was to evaluate the physicochemical, microbiological, and biochemical quality of commercially available dry-aged beef produced in Poland, with particular emphasis on differences between the crust and the interior of steaks. Fourteen samples (11 rib-eye and 3 sirloin) from different producers were analysed. The colour parameters, pH, water activity, thiobarbituric acid reactive substances, water-holding capacity, basic chemical composition, texture, microbial counts, free amino acids, biogenic amines profiles, and consumer sensory quality were determined. Microbiological analyses covered total plate count, psychrotrophic bacteria, lactic acid bacteria, Enterobacteriaceae, Pseudomonas spp., Brochothrix thermosphacta, yeasts and Moulds, as well as the presence of Salmonella spp. and Listeria monocytogenes. Fungal species isolated from selected crust samples were identified using ITS rDNA sequencing. Significant variability among products was observed in colour (L*: 31.1–45.0), pH (5.3–6.7), and water activity (0.92–0.99). Compared with the interior, the crust exhibited higher lipid oxidation (TBARS up to 2.31 mg MDA/kg), higher microbial loads, and greater accumulation of biogenic amines, including tyramine, cadaverine, and putrescine. Rib-eye 6, Rib-eye 7, Rib-eye 8, Rib-eye 10, and Rib-eye 11 showed the highest sensory acceptance. On the crust of selected highly rated steaks filamentous fungi such as Mucor flavus and Thamnidium elegans were identified, which suggests environmental colonisation and potential technological relevance. Full article