Search Results (14)

In the cultivation of maize, the leaf area index (LAI) serves as an important metric to determine the development of the plant. Unmanned aerial vehicles (UAVs) that capture RGB images, along with random forest regression (RFR), can be used to indirectly measure LAI through vegetative indices. Research using these techniques is at an early stage, especially in the context of maize for silage. Therefore, this study aimed to evaluate which vegetative indices have the strongest correlations with maize LAI and to compare two regression methods. RFR, ridge regression (RR), support vector machine (SVM), and multiple linear regression (MLR) regressions were performed in Python for comparison using images obtained in an area cultivated with maize for silage. The results showed that the RGB spectral indices showed saturation when the LAI reached 3 m² m⁻², with the VEG (vegetable index), COM (combination), ExGR (red–green excess), and TGI (triangular greenness index) indices selected for modeling. In terms of regression, RFR showed superior performance with an R² value of 0.981 and a root mean square error (RMSE) of 0.138 m² m⁻². Therefore, it can be concluded that RFR using RGB indices is a good way to indirectly obtain the LAI. Full article

Nowadays, Unmanned Aerial Systems (UASs) provide an efficient and relatively affordable remote sensing technology for assessing vegetation attributes and status across agricultural areas through wide-area imagery collected with cameras installed on board. This reduces the cost and time of crop monitoring at the field scale in comparison to conventional field surveys. In general, by using remote sensing-based approaches, information on crop conditions is obtained through the calculation and mapping of multispectral vegetation indices. However, some farmers are unable to afford the cost of multispectral images, while the use of RGB images could be a viable approach for monitoring the rice crop quickly and cost-effectively. Nevertheless, the suitability of RGB indices for this specific purpose is not yet well established and needs further investigation. The aim of this work is to explore the use of UAS-based RGB vegetation indices to monitor the rice crop. The study was conducted in a paddy area located in the Lis Valley (Central Portugal). The results revealed that the RGB indices, Visible Atmospherically Resistant Index (VARI) and Triangular Greenness Index (TGI) can be useful tools for rice crop monitoring in the absence of multispectral images, particularly in the late vegetative phase. Full article

The productivity of wheat in the Mediterranean region is under threat due to climate-change-related environmental factors, including fungal diseases that can negatively impact wheat yield and quality. Wheat phenotyping tools utilizing affordable, high-throughput plant phenotyping (HTPP) techniques, such as aerial and ground RGB images and quick canopy and leaf sensors, can aid in assessing crop status and selecting tolerant wheat varieties. This study focused on the impact of fungal diseases on wheat productivity in the Mediterranean region, considering the need for a precise selection of tolerant wheat varieties. This research examined the use of affordable HTPP methods, including imaging and active multispectral sensors, to aid in crop management for improved wheat health and to support commercial field phenotyping programs. This study evaluated 40 advanced lines of bread wheat (Triticum aestivum L.) at five locations across northern Spain, comparing fungicide-treated and untreated blocks under fungal disease pressure (Septoria, brown rust, and stripe rust observed). Measurements of leaf-level pigments and canopy vegetation indexes were taken using portable sensors, field cameras, and imaging sensors mounted on unmanned aerial vehicles (UAVs). Significant differences were observed in Dualex flavonoids and the nitrogen balance index (NBI) between treatments in some locations (p < 0.001 between Elorz and Ejea). Measurements of canopy vigor and color at the plot level showed significant differences between treatments at all sites, highlighting indexes such as the green area (GA), crop senescence index (CSI), and triangular greenness index (TGI) in assessing the effects of fungicide treatments on different wheat cultivars. RGB vegetation indexes from the ground and UAV were highly correlated (r = 0.817 and r = 0.810 for TGI and NGRDI). However, the Greenseeker NDVI sensor was found to be more effective in estimating grain yield and protein content (R² = 0.61–0.7 and R² = 0.45–0.55, respectively) compared to the aerial AgroCam GEO NDVI (R² = 0.25–0.35 and R² = 0.12–0.21, respectively). We suggest as a practical consideration the use of the GreenSeeker NDVI as more user-friendly and less affected by external environmental factors. This study emphasized the throughput benefits of RGB UAV HTPPs with the high similarity between ground and aerial results and highlighted the potential for HTPPs in supporting the selection of fungal-disease-resistant bread wheat varieties. Full article

The aim of the study was to evaluate the effect of various fertilization treatments, including nitrogen, potassium and phosphorus fertilization, in long-term experiments for selected UAV (unmanned aerial vehicle)-derived spectral vegetation indices (NDVI—Normalized Difference Vegetation Index, NDRE—Normalized Difference Red Edge Index, VARI—Visible Atmospherically Resistant Index, TGI—Triangular Greenness Index, SIPI2—Structure Insensitive Pigment Index 2, LCI—Leaf Chlorophyll Index, BNDVI—Blue Normalized Difference Vegetation Index, GNDVI—Green Normalized Difference Vegetation Index, MCARI—Modified Chlorophyll Absorption in Reflective Index) based on multispectral (bands in the range of visible light and near infra-red) images of winter rye. The strongest effect on the studied vegetation indices was nitrogen fertilization, which discriminated values of most of the vegetation indices. The effect of phosphorus and potassium fertilization on the studied vegetation indices was much weaker. The treatments with nitrogen fertilization had significantly higher values of most vegetation indices in comparison to treatments without nitrogen. This was confirmed by principal component analysis (PCA), in which treatments without nitrogen fertilization were very different in comparison to all other treatments where nitrogen fertilization was applied. The effect of phosphorus and potassium fertilization on most of vegetation indices was relatively weak and not significant in most experiments. Only for rye cultivated in monoculture was the effect of phosphorus fertilization significant for most of vegetation indices in early growth stages. In later growth stages (heading and flowering) the effect of phosphorus fertilization was significant in rye monoculture for the SIPI2 vegetation index. Mean SIPI2 was higher for the fertilization treatment CaNPK in comparison to CaKN (without P fertilization). The effect of potassium fertilization on the studied vegetation indices was very weak, and in most cases not significant. The effect of nitrogen fertilization on vegetation indices was much stronger than effect of both potassium and phosphorus fertilization. Full article

Significant losses in agricultural production are due to abiotic stresses, such as herbicide phytotoxicity. Dicamba (diglycolamine salt) is a herbicide used for post-emergent control of broadleaf weeds. It has a possibility to vapor-spread into neighboring fields causing damage to other crops. However, not every stress can be easily identified. Therefore, remote sensing has the potential as a new tool in early injury detection. This study evaluated the effects of simulated dicamba drift on the occurrence of phytotoxicity in soybeans (Glycine max). Soybean was assessed in seven dicamba doses (0, 0.056, 0.56, 5.6, 11.2, 28, 112 g ha⁻¹) for changes in plant injury (scale of notes), spectral aspects (triangular greenness index (TGI), and shoot dry mass. The plants were photographed using a digital camera positioned at 1.2 m above the planting media level. The results indicate a positive effect of low dicamba doses (0.56 and 0.056 g a.e. ha⁻¹) on TGI canopy distinction and shoot dry mass. Soybean TGI canopy distinction and the injury scale estimated at 45 days after sowing, and the soybean shoot dry mass observed at 99 days after sowing, presented significant and moderate Pearson’s r coefficient of correlations (r = −0.609 and 0.625), indicating TGI as a valid and practical spectral index for plant dicamba-injured evaluations. Full article

Soil fertility affects crop yield and quality. A quick, accurate evaluation of soil fertility is crucial for agricultural production. Few satellite image-based evaluation studies have quantified soil fertility during the crop growth period. Therefore, this study proposes a new approach to the quantitative evaluation of soil fertility. Firstly, the optimal crop spectral variables were selected using the integration of an extreme gradient boosting (XGBoost) algorithm with variance inflation factor (VIF). Then, based on the optimal crop spectral variables where the red-edge indices were introduced for the first time, the estimation models were developed using the backpropagation neural network (BPNN) algorithm to assess soil fertility. The model was finally adopted to map the soil fertility using Sentinel-2 imagery. This study was performed in the Conghua District of Guangzhou, Guangdong Province, China. The results of our research are as follows: (1) five crop spectral variables (inverted red-edge chlorophyll index (IRECI), chlorophyll vegetation index (CVI), normalized green-red difference index (NGRDI), red-edge position (REP), and triangular greenness index (TGI)) were the optimal variables. (2) The BPNN model established with optimal variables provided reliable estimates of soil fertility, with the determination coefficient (R²) of 0.66 and a root mean square error (RMSE) of 0.17. A nonlinear relation was found between soil fertility and the optimal crop spectral variables. (3) The BPNN model provides the potential for soil fertility mapping using Sentinel-2 images, with an R² of 0.62 and an RMSE of 0.09 for the measured and estimated results. This study suggests that the proposed method is suitable for the estimation of soil fertility in paddy fields. Full article

Multispectral images (MSIs) are valuable for precision agriculture due to the extra spectral information acquired compared to natural color RGB (ncRGB) images. In this paper, we thus aim to generate high spatial MSIs through a robust, deep-learning-based reconstruction method using ncRGB images. Using the data from the agronomic research trial for maize and breeding research trial for rice, we first reproduced ncRGB images from MSIs through a rendering model, Model-True to natural color image (Model-TN), which was built using a benchmark hyperspectral image dataset. Subsequently, an MSI reconstruction model, Model-Natural color to Multispectral image (Model-NM), was trained based on prepared ncRGB (ncRGB-Con) images and MSI pairs, ensuring the model can use widely available ncRGB images as input. The integrated loss function of mean relative absolute error (MRAE_loss) and spectral information divergence (SID_loss) were most effective during the building of both models, while models using the MRAE_loss function were more robust towards variability between growing seasons and species. The reliability of the reconstructed MSIs was demonstrated by high coefficients of determination compared to ground truth values, using the Normalized Difference Vegetation Index (NDVI) as an example. The advantages of using “reconstructed” NDVI over Triangular Greenness Index (TGI), as calculated directly from RGB images, were illustrated by their higher capabilities in differentiating three levels of irrigation treatments on maize plants. This study emphasizes that the performance of MSI reconstruction models could benefit from an optimized loss function and the intermediate step of ncRGB image preparation. The ability of the developed models to reconstruct high-quality MSIs from low-cost ncRGB images will, in particular, promote the application for plant phenotyping in precision agriculture. Full article

Coastal tidal marshes are essential ecosystems for both economic and ecological reasons. They necessitate regular monitoring as the effects of climate change begin to be manifested in changes to marsh vegetation healthiness. Small unmanned aerial systems (sUAS) build upon previously established remote sensing techniques to monitor a variety of vegetation health metrics, including biomass, with improved flexibility and affordability of data acquisition. The goal of this study was to establish the use of RGB-based vegetation indices for mapping and monitoring tidal marsh vegetation (i.e., Spartina alterniflora) biomass. Flights over tidal marsh study sites were conducted using a multi-spectral camera on a quadcopter sUAS near vegetation peak growth. A number of RGB indices were extracted to build a non-linear biomass model. A canopy height model was developed using sUAS-derived digital surface models and LiDAR-derived digital terrain models to assess its contribution to the biomass model. Results found that the distance-based RGB indices outperformed the regular radio-based indices in coastal marshes. The best-performing biomass models used the triangular greenness index (TGI; R² = 0.39) and excess green index (ExG; R² = 0.376). The estimated biomass revealed high biomass predictions at the fertilized marsh plots in the Long-Term Research in Environmental Biology (LTREB) project at the study site. The sUAS-extracted canopy height was not statistically significant in biomass estimation but showed similar explanatory power to other studies. Due to the lack of biomass samples in the inner estuary, the proposed biomass model in low marsh does not perform as well as the high marsh that is close to shore and accessible for biomass sampling. Further research of low marsh is required to better understand the best conditions for S. alterniflora biomass estimation using sUAS as an on-demand, personal remote sensing tool. Full article

The consolidation of unmanned aerial vehicle (UAV) photogrammetric techniques for campaigns with high and medium observation scales has triggered the development of new application areas. Most of these vehicles are equipped with common visible-band sensors capable of mapping areas of interest at various spatial resolutions. It is often necessary to identify vegetated areas for masking purposes during the postprocessing phase, excluding them for the digital elevation models (DEMs) generation or change detection purposes. However, vegetation can be extracted using sensors capable of capturing the near-infrared part of the spectrum, which cannot be recorded by visible (RGB) cameras. In this study, after reviewing different visible-band vegetation indices in various environments using different UAV technology, the influence of the spatial resolution of orthomosaics generated by photogrammetric processes in the vegetation extraction was examined. The triangular greenness index (TGI) index provided a high level of separability between vegetation and nonvegetation areas for all case studies in any spatial resolution. The efficiency of the indices remained fundamentally linked to the context of the scenario under investigation, and the correlation between spatial resolution and index incisiveness was found to be more complex than might be trivially assumed. Full article

The recent availability of soybean cultivars with resistance to dicamba herbicide has increased the risk of injury in susceptible cultivars, mainly as a result of particle drift. To predict and identify the damage caused by this herbicide requires great accuracy. The objective of this work was to evaluate the injury caused by the simulated drift of dicamba on soybean (nonresistant to dicamba) plants assessed visually and using the Triangular Greenness Index (TGI) from images obtained from Remotely Piloted Aircraft (RPA). The study was conducted in a randomized complete block design with four replications during the 2019/2020 growing season, and the treatments consisted of the application of six doses of dicamba (0, 0.28, 0.56, 5.6, 28, and 112 g acid equivalent dicamba ha⁻¹) on soybean plants at the third node growth stage. For the evaluation of treatments using the TGI technique, spectral data acquired through a Red Green Blue (RGB) sensor attached to an RPA was used. The variables studied were the visual estimation of injury, TGI response at 7 and 21 days after application, plant height, and crop yield. The exposure to the herbicide caused a reduction in plant height and crop yield. Vegetation indices, such as TGI, have the potential to be used in the evaluation of injury caused by dicamba, and may be used to cover large areas in a less subjective way than visual assessments. Full article

New management practices must be developed to improve yam productivity. By allowing non-destructive analyses of important plant traits, image-based phenotyping techniques could help developing such practices. Our objective was to determine the potential of image-based phenotyping methods to assess traits relevant for tuber yield formation in yam grown in the glasshouse and in the field. We took plant and leaf pictures with consumer cameras. We used the numbers of image pixels to derive the shoot biomass and the total leaf surface and calculated the ‘triangular greenness index’ (TGI) which is an indicator of the leaf chlorophyll content. Under glasshouse conditions, the number of pixels obtained from nadir view (view from the top) was positively correlated to shoot biomass, and total leaf surface, while the TGI was negatively correlated to the SPAD values and nitrogen (N) content of diagnostic leaves. Pictures taken from nadir view in the field showed an increase in soil surface cover and a decrease in TGI with time. TGI was negatively correlated to SPAD values measured on diagnostic leaves but was not correlated to leaf N content. In conclusion, these phenotyping techniques deliver relevant results but need to be further developed and validated for application in yam. Full article

Corn yields vary spatially and temporally in the plots as a result of weather, altitude, variety, plant density, available water, nutrients, and planting date; these are the main factors that influence crop yield. In this study, different multispectral and red-green-blue (RGB) vegetation indices were analyzed, as well as the digitally estimated canopy cover and plant density, in order to estimate corn grain yield using a neural network model. The relative importance of the predictor variables was also analyzed. An experiment was established with five levels of nitrogen fertilization (140, 200, 260, 320, and 380 kg/ha) and four replicates, in a completely randomized block design, resulting in 20 experimental polygons. Crop information was captured using two sensors (Parrot Sequoia_4.9, and DJI FC6310_8.8) mounted on an unmanned aerial vehicle (UAV) for two flight dates at 47 and 79 days after sowing (DAS). The correlation coefficient between the plant density, obtained through the digital count of corn plants, and the corn grain yield was 0.94; this variable was the one with the highest relative importance in the yield estimation according to Garson’s algorithm. The canopy cover, digitally estimated, showed a correlation coefficient of 0.77 with respect to the corn grain yield, while the relative importance of this variable in the yield estimation was 0.080 and 0.093 for 47 and 79 DAS, respectively. The wide dynamic range vegetation index (WDRVI), plant density, and canopy cover showed the highest correlation coefficient and the smallest errors (R = 0.99, mean absolute error (MAE) = 0.028 t ha⁻¹, root mean square error (RMSE) = 0.125 t ha⁻¹) in the corn grain yield estimation at 47 DAS, with the WDRVI index and the density being the variables with the highest relative importance for this crop development date. For the 79 DAS flight, the combination of the normalized difference vegetation index (NDVI), normalized difference red edge (NDRE), WDRVI, excess green (EXG), triangular greenness index (TGI), and visible atmospherically resistant index (VARI), as well as plant density and canopy cover, generated the highest correlation coefficient and the smallest errors (R = 0.97, MAE = 0.249 t ha⁻¹, RMSE = 0.425 t ha⁻¹) in the corn grain yield estimation, where the density and the NDVI were the variables with the highest relative importance, with values of 0.295 and 0.184, respectively. However, the WDRVI, plant density, and canopy cover estimated the corn grain yield with acceptable precision (R = 0.96, MAE = 0.209 t ha⁻¹, RMSE = 0.449 t ha⁻¹). The generated neural network models provided a high correlation coefficient between the estimated and the observed corn grain yield, and also showed acceptable errors in the yield estimation. The spectral information registered through remote sensors mounted on unmanned aerial vehicles and its processing in vegetation indices, canopy cover, and plant density allowed the characterization and estimation of corn grain yield. Such information is very useful for decision-making and agricultural activities planning. Full article

Huanglongbing (HLB) and Phytophthora foot and root rot are diseases that affect citrus production and profitability. The symptoms and physiological changes associated with these diseases are diagnosed through expensive and time-consuming field measurements. Unmanned aerial vehicles (UAVs) using red/green/blue (RGB, true color) imaging, may be an economic alternative to diagnose diseases. A methodology using a UAV with a RGB camera was developed to assess citrus health. The UAV was flown in April 2018 on a grapefruit field infected with HLB and foot rot. Ten trees were selected for each of the following disease classifications: (HLB-, foot rot–), (HLB+, foot rot–), (HLB-, foot rot+) (HLB+, foot rot+). Triangular greenness index (TGI) images were correlated with field measurements such as tree nutritional status, leaf area, SPAD (leaf greenness), foot rot disease severity and HLB. It was found that 61% of the TGI differences could be explained by Na, Fe, foot rot, Ca, and K. This study shows that diseased citrus trees can be monitored using UAVs equipped with RGB cameras, and that TGI can be used to explain subtle differences in tree health caused by multiple diseases. Full article

Verticillium Wilt of Olive, a disease caused by the hemibiotrophic vascular fungus Verticillium dahliae Kleb. presents one of the most important constraints to olive production in the world, with an especially notable impact in Mediterranean agriculture. This study evaluates the use of RGB vegetation indexes in assessing the effects of this disease during the biotrophic phase of host-pathogen interaction, in which symptoms of wilt are not yet evident. While no differences were detected by measuring stomatal conductance and chlorophyll fluorescence, results obtained from RGB indexes showed significant differences between control and inoculated plants for indexes Saturation, a*, b*, green Area (GA), normalized green-red difference index (NGRDI) and triangular greenness index (TGI), presenting a reduction in plant growth as well as in green and yellow color components as an effect of inoculation. These results were contrasted across two scenarios of mineral fertilization in soil and soil amended with two different olive mill waste composts, presenting a clear interaction between the host-pathogen relationship and plant nutrition and suggesting the effect of V. dahliae infection during the biotrophic phase was not related to plant water status. Full article