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21 pages, 7055 KB  
Article
The Effect of Polymer Fiber Reinforcement on the Structural Performance of Timber Columns Under Axial Compression
by Haifa Abuhliga and Tahir Akgül
Buildings 2026, 16(3), 479; https://doi.org/10.3390/buildings16030479 - 23 Jan 2026
Viewed by 101
Abstract
This study investigates the compressive behavior of glued-laminated timber (Glulam) columns reinforced with different configurations of fiber-reinforced polymer (FRP) materials, including glass (GFRP) and carbon (CFRP) fibers in the form of rods, strip/panel, and fabrics. Axial compression tests were performed under controlled laboratory [...] Read more.
This study investigates the compressive behavior of glued-laminated timber (Glulam) columns reinforced with different configurations of fiber-reinforced polymer (FRP) materials, including glass (GFRP) and carbon (CFRP) fibers in the form of rods, strip/panel, and fabrics. Axial compression tests were performed under controlled laboratory conditions to examine the influence of reinforcement type and configuration on mechanical performance. Descriptive statistics, one-way ANOVA, and Tukey’s post hoc tests were used to determine the significance of differences between the tested groups. Finite element analysis (FEA) using ANSYS software2023 R1 was also conducted to validate the experimental results and to provide insight into stress distribution within the strengthened columns. The results revealed that FRP reinforcement clearly enhanced both the ultimate load and compressive stress compared to unreinforced samples. The highest performance was achieved with double CFRP rods and 5 cm carbon strips, which reached stress levels of about 43 MPa, representing an improvement of nearly 60% over raw wood. Statistical analysis confirmed that these increases were significant (p < 0.05), while FEA predictions showed strong agreement with the experimental findings. Observed failure modes shifted from crushing and buckling in unreinforced specimens to shear-splitting and delamination in reinforced ones, indicating improved confinement and delayed failure. Full article
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19 pages, 2346 KB  
Article
Process Simulation of a Temperature Swing Absorption Process for Hydrogen Isotope Separation
by Annika Uihlein, Jonas Caspar Schwenzer, Stefan Hanke and Thomas Giegerich
Energies 2026, 19(2), 466; https://doi.org/10.3390/en19020466 - 17 Jan 2026
Viewed by 117
Abstract
Temperature Swing Absorption (TSA) is the primary candidate for the Isotope Rebalancing and Protium Removal (IRPR) system within the envisioned EU-DEMO fusion reactor fuel cycle. TSA separates a mixed hydrogen isotope stream into two product streams using a semi-continuous process. One stream, enriched [...] Read more.
Temperature Swing Absorption (TSA) is the primary candidate for the Isotope Rebalancing and Protium Removal (IRPR) system within the envisioned EU-DEMO fusion reactor fuel cycle. TSA separates a mixed hydrogen isotope stream into two product streams using a semi-continuous process. One stream, enriched in heavy isotopes, is used to re-establish the required deuterium-to-tritium fuel ratio. The second, enriched in protium, is stripped off from the fuel cycle to counteract the protium build-up. Separation is achieved by cycling an isotope mixture between two columns filled with metallic absorption materials that have opposite isotope effects of metal hydride formation. The selection of these materials, the operation parameters and the column geometry allow for adjusting the resulting enrichments. To identify suitable operation parameters, a TSA process model is developed which depicts the process dynamics and interactions between the columns. A modified process operation mode is introduced, which enables higher system throughputs and non-cryogenic operation, i.e., operational temperatures between 0 to 130 °C, while reducing the tritium inventory due to shorter cycling times by reduced amplitudes of the temperature swings. Finally, simulations of a TSA system at relevant scale confirm the suitability of TSA technology for the separation task of the EU-DEMO IRPR system. Full article
(This article belongs to the Section B4: Nuclear Energy)
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14 pages, 2414 KB  
Article
An Integrated Analytical and Extended Ponchon–Savarit Graphical Method for Determining Actual and Minimum Boil-Up Ratios in Binary Distillation
by Oualid Hamdaoui
Processes 2025, 13(10), 3031; https://doi.org/10.3390/pr13103031 - 23 Sep 2025
Viewed by 1397
Abstract
A rigorous framework for determining actual and minimum boil-up ratios in binary distillation combining analytical mass and energy balances with an extended Ponchon–Savarit graphical approach was implemented. First, global balances across the enriching and stripping sections yield a closed-form expression of the boil-up [...] Read more.
A rigorous framework for determining actual and minimum boil-up ratios in binary distillation combining analytical mass and energy balances with an extended Ponchon–Savarit graphical approach was implemented. First, global balances across the enriching and stripping sections yield a closed-form expression of the boil-up ratio (VB) based on enthalpy differences. Second, the VB was directly determined from an enthalpy–composition diagram by measuring the enthalpy segments between the saturated liquid, vapor, and heat-duty points. Applying this method to high-stage columns confirms that the two methods converge on identical VB values. Based on these findings, a unified graphical methodology was developed to determine the minimum boil-up ratio (VBmin). VBmin can be determined on the same diagram by locating the intersections of the extremal tie lines in both the enriching and exhausting sections, analogous to the reflux-pinch points. This procedure was systematically validated across the five canonical feed thermal states. The implemented method is a graphical approach based on the Ponchon–Savarit technique, developed for binary systems. Full article
(This article belongs to the Section Separation Processes)
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13 pages, 1789 KB  
Article
Simulation of Pantograph–Catenary Arc Temperature Field in Urban Railway and Study of Influencing Factors on Arc Temperature
by Xiaoying Yu, Yang Su, Mengjie Song, Junrui Yang, Liying Song, Ze Wang, Yixiao Liu, Caizhuo Wei and Yongjia Cheng
Infrastructures 2025, 10(9), 237; https://doi.org/10.3390/infrastructures10090237 - 10 Sep 2025
Cited by 2 | Viewed by 704
Abstract
During the running of urban railway trains, arcs of the pantograph–catenary (PC) system cause instantaneous high-temperature ablation of PC system materials, which severely impact the standard running of trains. Utilizing magnetohydrodynamics (MHD), a mathematical model of urban railway PC arcs is introduced in [...] Read more.
During the running of urban railway trains, arcs of the pantograph–catenary (PC) system cause instantaneous high-temperature ablation of PC system materials, which severely impact the standard running of trains. Utilizing magnetohydrodynamics (MHD), a mathematical model of urban railway PC arcs is introduced in this article. The multiphysics finite element analysis platform COMSOL Multiphysics was used to solve and simulate the mathematical model of the PC arc. The simulation results were analyzed to explore the temperature dispersion law of the PC arc. Experimental measurements of arc duration and arc temperature were conducted, with the mathematical model’s accuracy validated through empirical comparisons. Based on the established mathematical model of the PC arc, the effects of PC gap and current intensity on the arc temperature were investigated. The results reveal that the PC arc’s temperature field follows a radially decaying dispersion, attaining maximum temperature in the center of the arc column. The surface temperature of the pantograph strip is higher than that of the contact wire. As the duration of the PC arc increases, the arc temperature gradually increases; the temperature of the PC arc diminishes with the increase in the PC gap. The PC current increases, and the arc zone temperature increases. The research conclusions of this article can provide a basis for mitigating the number of PC arcs and enhancing the quality of the PC current. Full article
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14 pages, 3153 KB  
Article
The Analysis of Axial Compression Performance of Reinforced Concrete Columns Strengthened with Prestressed Carbon Fiber Sheets
by Yiquan Lv, Yang Teng, Xing Li, Junli Liu, Chunling Lu and Cheng Zhang
Infrastructures 2025, 10(8), 210; https://doi.org/10.3390/infrastructures10080210 - 13 Aug 2025
Viewed by 822
Abstract
Current research primarily focuses on using CFRP materials to strengthen small or medium-sized test specimens. To address this, our study employed ABAQUS software to analyze the axial compression behavior of large-scale reinforced concrete (RC) columns strengthened with prestressed carbon fiber reinforced polymer (CFRP) [...] Read more.
Current research primarily focuses on using CFRP materials to strengthen small or medium-sized test specimens. To address this, our study employed ABAQUS software to analyze the axial compression behavior of large-scale reinforced concrete (RC) columns strengthened with prestressed carbon fiber reinforced polymer (CFRP) sheets. We conducted comparative analyses on key parameters: the prestress level applied to the CFRP, the width of CFRP strips, the spacing between strips, the confinement ratio, and the overall load–displacement curves of the columns. The results demonstrate that applying prestress significantly improves the efficiency of stress transfer in the CFRP sheet, effectively mitigating the stress lag phenomenon common in traditional CFRP strengthening, leading to a substantially enhanced strengthening effect. The CFRP wrapping method critically impacts performance: increasing the confinement ratio enhanced ultimate load capacity by 21.8–59.9%; reducing the strip spacing increased capacity by 21.8–50.4%; and widening the strips boosted capacity by 38.7–58%. Although full wrapping achieved the highest capacity increase (up to 73.2%), it also incurred significantly higher costs. To ensure the required strengthening effect while optimizing economic efficiency and CFRP material utilization, the strip wrapping technique is recommended. For designing optimal reinforcement, priority should be given to optimizing the confinement ratio first, followed by adjusting strip width and spacing. Proper optimization of these parameters significantly enhances the strengthened member’s ultimate load capacity, ductility, and energy dissipation capacity. This study enriches the theoretical foundation for prestressed CFRP strengthening and provides an essential basis for rationally selecting prestress levels and layout parameters in engineering practice, thereby aiding the efficient design of strengthening projects for structures like bridges, with significant engineering and scientific value. Full article
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23 pages, 3551 KB  
Article
The Influence of Soft Soil, Pile–Raft Foundation and Bamboo on the Bearing Characteristics of Reinforced Concrete (RC) Structure
by Zhibin Zhong, Xiaotong He, Shangheng Huang, Chao Ma, Baoxian Liu, Zhile Shu, Yineng Wang, Kai Cui and Lining Zheng
Buildings 2025, 15(13), 2302; https://doi.org/10.3390/buildings15132302 - 30 Jun 2025
Cited by 1 | Viewed by 2371
Abstract
Pile–raft foundations are widely used in soft soil engineering due to their good integrity and high stiffness. However, traditional design methods independently design pile–raft foundations and superstructures, ignoring their interaction. This leads to significant deviations from actual conditions when the superstructure height increases, [...] Read more.
Pile–raft foundations are widely used in soft soil engineering due to their good integrity and high stiffness. However, traditional design methods independently design pile–raft foundations and superstructures, ignoring their interaction. This leads to significant deviations from actual conditions when the superstructure height increases, resulting in excessive costs and adverse effects on building stability. This study experimentally investigates the interaction characteristics of pile–raft foundations and superstructures in soft soil under different working conditions using a 1:10 geometric similarity model. The superstructure is a cast-in-place frame structure (beams, columns, and slabs) with bamboo skeletons with the same cross-sectional area as the piles and rafts, cast with concrete. The piles in the foundation use rectangular bamboo strips (side length ~0.2 cm) instead of steel bars, with M1.5 mortar replacing C30 concrete. The raft is also made of similar materials. The results show that the soil settlement significantly increases under the combined action of the pile–raft and superstructure with increasing load. The superstructure stiffness constrains foundation deformation, enhances bearing capacity, and controls differential settlement. The pile top reaction force exhibits a logarithmic relationship with the number of floors, coordinating the pile bearing performance. Designers should consider the superstructure’s constraint of the foundation deformation and strengthen the flexural capacity of inner pile tops and bottom columns for safety and economy. Full article
(This article belongs to the Section Building Structures)
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22 pages, 7385 KB  
Article
Axial Performances of CFRP-PVC Confined RAC Columns: Experimental and Numerical Study
by Zidong Hu, Ruoyu Cao, Qiaoyun Wu, Cheng Zhao, Jie Li and Xuyong Chen
Buildings 2025, 15(12), 2089; https://doi.org/10.3390/buildings15122089 - 17 Jun 2025
Viewed by 701
Abstract
The use of recycled aggregate concrete (RAC) in construction mitigates environmental pollution by repurposing demolition waste, but its lower compressive strength compared to natural aggregate concrete (NAC) limits broader application. Although carbon fiber reinforced polymer (CFRP) composites and polyvinyl chloride (PVC) tubes have [...] Read more.
The use of recycled aggregate concrete (RAC) in construction mitigates environmental pollution by repurposing demolition waste, but its lower compressive strength compared to natural aggregate concrete (NAC) limits broader application. Although carbon fiber reinforced polymer (CFRP) composites and polyvinyl chloride (PVC) tubes have individually been shown to improve concrete strength and ductility, existing studies focus on fully wrapped CFRP jackets on NAC columns and do not systematically explore CFRP–PVC hybrid confinement using strips on RAC. To address this research gap, this study investigates the axial compressive behavior of CFRP–PVC–RAC columns by varying CFRP strip width (from 25 to 75 mm), strip spacing (from 31 to 77.5 mm), and the number of CFRP layers (one to nine) over a central PVC tube. Axial compression tests reveal that specimens with a central CFRP strip width equal to or greater than 75 mm achieve peak loads up to 1331 kN and that, after rupture of the central strip, the remaining strips continue to carry load, producing a more gradual stress–strain decline and enhanced ductility compared to fully wrapped controls (peak load 1219 kN). These results show that CFRP–PVC composites enhance the axial compressive strength and ductility of RAC columns. The confinement mechanism increases the ultimate axial strain and redistributes transverse stresses, delaying brittle failure and improving deformation capacity. When two or more CFRP layers are applied, strip width and spacing affect axial stress by no more than three percent. Increasing layers from one to four raises axial strength by approximately 23 percent, whereas adding layers beyond four yields diminishing returns, with less than a six percent increase. Finally, a multilayer lateral confined pressure formula is derived and validated against thirty-two specimens, exhibiting errors no greater than three percent and accurately predicting effective confinement. These findings offer practical guidance for optimizing strip dimensions and layering in CFRP–PVC reinforcement of RAC columns, achieving material savings without compromising performance. Full article
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22 pages, 3010 KB  
Article
Seismic Performance Research of Self-Centering Single-Column Bridges Using Equivalent Stiffness Theory
by Huixing Gao, Wenjing Xia and Hongxu Lu
Buildings 2025, 15(12), 2000; https://doi.org/10.3390/buildings15122000 - 10 Jun 2025
Viewed by 709
Abstract
Single-column hybrid-reinforced self-centering segmental assembled bridges (SHR-SCSAB) exhibit vertical stiffness discontinuities, significantly impacting the refinement of their seismic design methodology. In this study, we investigate SHR-SCSAB by employing the finite strip method to calculate the maximum transverse bearing capability of segmental assembled piers, [...] Read more.
Single-column hybrid-reinforced self-centering segmental assembled bridges (SHR-SCSAB) exhibit vertical stiffness discontinuities, significantly impacting the refinement of their seismic design methodology. In this study, we investigate SHR-SCSAB by employing the finite strip method to calculate the maximum transverse bearing capability of segmental assembled piers, and the corresponding horizontal displacement at the pier top. By leveraging the mechanical properties of hybrid reinforcement materials, we further derive an analytical expression for the equivalent elastic stiffness of SHR-SCSAB as an integrated system. OpenSees software was used to establish a finite element model of the SHR-SCSAB, and the agreement between numerical simulations and analytical solutions validates the accuracy of the derived equivalent elastic stiffness expression. Additionally, this study evaluates the seismic performance of single-column SHR-SCSAB and examines the influence of key parameters on its behavior. The results demonstrate that hybrid reinforcement effectively addresses the low energy dissipation capacity inherent in self-centering bridges while preserving their advantage of minimal residual displacement. These findings significantly advance the refinement of seismic design methods for SHR-SCSAB. Full article
(This article belongs to the Section Building Structures)
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19 pages, 4901 KB  
Article
Study on Seismic Performance of Reinforced Concrete Columns Reinforced with Steel Strip Composite Ultra–High–Performance Concrete
by Xianhui Liu, Wenlong Chang, Zihang Wang and Meiqing Pan
Buildings 2025, 15(11), 1762; https://doi.org/10.3390/buildings15111762 - 22 May 2025
Cited by 1 | Viewed by 2442
Abstract
To enhance the seismic performance of existing reinforced concrete (RC) columns, this study proposes a novel strengthening method that combines steel strips with ultra–high–performance concrete (UHPC). The seismic behavior of the proposed method is investigated through quasi–static cyclic tests conducted on four strengthened [...] Read more.
To enhance the seismic performance of existing reinforced concrete (RC) columns, this study proposes a novel strengthening method that combines steel strips with ultra–high–performance concrete (UHPC). The seismic behavior of the proposed method is investigated through quasi–static cyclic tests conducted on four strengthened columns and one control column. The experimental parameters include the type of reinforcement (UHPC–only and UHPC combined with steel strips) and the thickness of the UHPC strengthening layer. The failure modes, hysteretic behavior, energy dissipation capacity, and stiffness degradation of the specimens are systematically analyzed. The results show that, compared to the unstrengthened column, the UHPC–strengthened columns achieved maximum increases of 73.73% in peak load and 23.68% in ductility coefficient, while the columns strengthened with composite steel strips achieved further improvements of up to 84.79% and 50.23%, respectively. The composite strengthening method significantly improved the failure mode, with crack distribution changing from localized crushing to multiple fine cracks. The displacement ductility coefficient reached as high as 6.28, and the hysteretic curve fullness and cumulative energy dissipation increased by a factor of two to three. Finally, based on moment equilibrium theory, a theoretical formula is proposed to calculate the lateral ultimate flexural capacity of RC columns strengthened with steel strip–UHPC composites, which shows good agreement with the experimental results. Full article
(This article belongs to the Section Building Structures)
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23 pages, 16876 KB  
Article
Compression Molding Flow Behavior and Void Optimization of an Integrated Circuit Package with Shielding-Metal-Frame
by Ting-Yu Lee, Yu-Li Chen, Sheng-Jye Hwang, Wei-Lun Cheng and Chun-Yu Ko
Polymers 2025, 17(10), 1301; https://doi.org/10.3390/polym17101301 - 9 May 2025
Cited by 1 | Viewed by 1822
Abstract
As the demand for smaller and more multifunctional integrated circuit (IC) products increases, system-in-package (SiP) has emerged as a key trend in IC encapsulation. However, the use of polymer-based materials such as epoxy molding compounds (EMCs) introduces complex flow behaviors during the encapsulation [...] Read more.
As the demand for smaller and more multifunctional integrated circuit (IC) products increases, system-in-package (SiP) has emerged as a key trend in IC encapsulation. However, the use of polymer-based materials such as epoxy molding compounds (EMCs) introduces complex flow behaviors during the encapsulation process, often leading to void formation, especially in highly integrated SiP structures. This study employs the Moldex3D 2024 R3 simulation software to perform mold-filling analyses of SiP packages using EMC as the encapsulant. The objective is to investigate why voids are consistently observed in the leftmost column of the strip and to determine how to reduce the void size using the Taguchi optimization method. To replicate the actual vacuum-assisted molding conditions, a 1/5 strip model with venting was established. Results show that the flow dynamics of the polymeric encapsulant are significantly affected by shielding frame geometry. Among various design modifications, adding an additional column of shielding metal frame on the left side of the strip most effectively reduces void formation. This research highlights the importance of polymer flow behavior in void prediction and optimization for advanced SiP packaging, providing practical guidelines for material-driven design improvements in IC encapsulation processes. Full article
(This article belongs to the Section Polymer Processing and Engineering)
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20 pages, 4654 KB  
Article
A Comparative Investigation on Axial and Flexural Performance of Circularized Square RC Columns with Discontinuous and Continuous CFRP Confinement
by Anh Duc Mai, Hoang Nam Phan, Quynh Chau Truong, Cong Luyen Nguyen and Quang Trung Nguyen
Buildings 2025, 15(8), 1228; https://doi.org/10.3390/buildings15081228 - 9 Apr 2025
Cited by 2 | Viewed by 790
Abstract
This paper aims to comprehensively investigate the axial and flexural performance of circularized square reinforced concrete (CSRC) columns discontinuously and continuously confined with carbon fiber-reinforced polymer (CFRP). The test results of twenty reinforced concrete (RC) columns, consisting of twelve CSRC columns and eight [...] Read more.
This paper aims to comprehensively investigate the axial and flexural performance of circularized square reinforced concrete (CSRC) columns discontinuously and continuously confined with carbon fiber-reinforced polymer (CFRP). The test results of twenty reinforced concrete (RC) columns, consisting of twelve CSRC columns and eight square RC (SRC) columns, are presented to compare the axial and flexural performance of discontinuously CFRP-confined CSRC (CFRPC-CSRC) columns with those of continuously CFRPC-CSRC and CFRPC-SRC columns. It was found that to enhance the load-bearing capacity (LBC) of SRC columns, circularizing the SRC columns before applying either discontinuous or continuous FRP confinement was more effective than applying continuous FRP confinement alone. Additionally, the theoretical strength interaction diagrams of test columns were developed using the strip-by-strip method, showing a strong agreement with the experimental results. Full article
(This article belongs to the Section Building Structures)
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19 pages, 5617 KB  
Article
Effect of Leaching on Particle Migration and Pore Structure of Ionic Rare Earth Ores with Different Fine Particle Contents
by Yunzhang Rao, Jiazheng Wan, Shujun Tan, Zhihua Yang, Guozhu Rao, Qiang Huang, Yangjun Xie and Qiande Lai
Metals 2025, 15(4), 396; https://doi.org/10.3390/met15040396 - 1 Apr 2025
Cited by 1 | Viewed by 995
Abstract
In in situ leaching, fine particles can be stripped and transported with the leach solution, significantly altering the particle size distribution and pore structure of each layer of the rare earth ore body. In this study, water and magnesium sulfate were used as [...] Read more.
In in situ leaching, fine particles can be stripped and transported with the leach solution, significantly altering the particle size distribution and pore structure of each layer of the rare earth ore body. In this study, water and magnesium sulfate were used as leaching agents. Based on indoor column leaching experiments, particle gradation experiments, and pore structure tests, this study investigates and analyzes the patterns of particle migration and changes in pore structure in rare earth ores with varying fine particle contents under leaching conditions. The results indicate that during the leaching process, the degree of change in particle gradation follows the order of upper layer > middle layer > lower layer. As the depth increases, the soil becomes denser, leading to reduced permeability, a slower seepage rate of the leaching solution, and a higher fine particle content, making the effect more pronounced. During magnesium sulfate leaching, the overall trend of porosity in the rare earth ore structure initially increases and then decreases. Additionally, a higher fine particle content corresponds to higher porosity. In the early and late stages of leaching, pore size changes involve the transformation of larger pores into smaller ones, followed by the conversion of smaller pores into larger ones. Moreover, the higher the fine particle content, the greater the degree of transformation between the pore sizes. Full article
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26 pages, 2696 KB  
Article
The Distribution of Dissolved Copper and Natural Organic Ligands in Tropical Coastal Waters Under Seasonal Variation
by Li Qing Ng, Khairul Nizam Mohamed, Abd Muhaimin Amiruddin, Ferdaus Mohamat Yusuff and Nur Ili Hamizah Mustaffa
J. Mar. Sci. Eng. 2025, 13(3), 446; https://doi.org/10.3390/jmse13030446 - 26 Feb 2025
Cited by 1 | Viewed by 1069
Abstract
The bioavailability of dissolved copper (Cu) in seawater is influenced by the presence of natural organic matter. Changes in physicochemical conditions, such as pH, temperature, and salinity, can significantly affect the solubility and speciation of copper, thereby impacting the complexation of Cu(II)-binding organic [...] Read more.
The bioavailability of dissolved copper (Cu) in seawater is influenced by the presence of natural organic matter. Changes in physicochemical conditions, such as pH, temperature, and salinity, can significantly affect the solubility and speciation of copper, thereby impacting the complexation of Cu(II)-binding organic ligands. The concentration of dissolved Cu in the coastal water of Mersing, Malaysia, was detected by anodic stripping voltammetry (ASV). The natural organic copper(II)-binding ligands (CuL) and their conditional stability constants (log K′) were determined by using the competitive ligand exchange–adsorptive cathodic stripping voltammetry method (CLE–AdCSV) in our samples. The in situ parameters, such as pH, temperature, salinity, and dissolved oxygen (DO), were found to be significantly different between sampling periods and indicated the different physical chemical conditions between the sampling periods. However, we found a consistent concentration of dissolved Cu throughout the water column between sampling periods. This suggests that the presence of a strong class of natural organic ligands (L1) in Mersing’s coastal water maintains the dissolved Cu(II) ions in the water column and prevents the scavenging and precipitation processes under the seasonal variations. Full article
(This article belongs to the Section Marine Environmental Science)
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26 pages, 3677 KB  
Article
Application of Pseudoinfectious Viruses in Transient Gene Expression in Mammalian Cells: Combining Efficient Expression with Regulatory Compliance
by Gulzat Zauatbayeva, Tolganay Kulatay, Bakytkali Ingirbay, Zhanar Shakhmanova, Viktoriya Keyer, Mikhail Zaripov, Maral Zhumabekova and Alexandr V. Shustov
Biomolecules 2025, 15(2), 274; https://doi.org/10.3390/biom15020274 - 13 Feb 2025
Viewed by 2640
Abstract
Transient gene expression (TGE) is commonly employed for protein production, but its reliance on plasmid transfection makes it challenging to scale up. In this paper, an alternative TGE method is presented, utilizing pseudoinfectious alphavirus as an expression vector. Pseudoinfectious viruses (PIV) and a [...] Read more.
Transient gene expression (TGE) is commonly employed for protein production, but its reliance on plasmid transfection makes it challenging to scale up. In this paper, an alternative TGE method is presented, utilizing pseudoinfectious alphavirus as an expression vector. Pseudoinfectious viruses (PIV) and a replicable helper construct were derived from the genome of the Venezuelan equine encephalitis virus. The PIV carries a mutant capsid protein that prevents packaging into infectious particles, while the replicable helper encodes a wild-type capsid protein but lacks other viral structural proteins. Although PIV and the helper cannot independently spread infection, their combination results in increased titers in cell cultures, enabling easier scale-up of producing cultures. The PIV-driven production of a model protein outperforms that of alphavirus replicon vectors or simple plasmid vectors. Another described feature of the expression system is the modification to immobilized metal affinity chromatography (IMAC), allowing purification of His-tagged recombinant proteins from a conditioned medium in the presence of substances that can strip metal from the IMAC columns. The PIV-based expression system allows for the production of milligram quantities of recombinant proteins in static cultures, without the need for complex equipment such as bioreactors, and complies with regulatory requirements due to its distinction from common recombinant viruses. Full article
(This article belongs to the Section Synthetic Biology and Bioengineering)
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14 pages, 8824 KB  
Article
Thermoplastic Phenomena and Morphological Changes upon Fast Pyrolysis of Biomass and Model Compounds
by Francesca Cerciello, Christophe Allouis, Carmela Russo, Erik Freisewinkel, David Tarlinski, Barbara Apicella, Martin Schiemann, Viktor Scherer and Osvalda Senneca
Molecules 2025, 30(3), 700; https://doi.org/10.3390/molecules30030700 - 5 Feb 2025
Cited by 3 | Viewed by 1288
Abstract
The work reports preliminary results on the morphological changes that biomass particles experience at high heating rates in a heated strip reactor (HSR) at T = 1000–1600 °C under an inert atmosphere. Samples included a natural lignocellulosic biomass (pinewood) as well as biomass [...] Read more.
The work reports preliminary results on the morphological changes that biomass particles experience at high heating rates in a heated strip reactor (HSR) at T = 1000–1600 °C under an inert atmosphere. Samples included a natural lignocellulosic biomass (pinewood) as well as biomass components: cellulose, hemicellulose (xylan) and lignin. On top of that, reference compounds have been investigated, namely naphthalene pitch, a paraffinic wax and glucose. During the heat-up phase, the investigated biomass mainly retains the original morphology and size, while the single components exhibit different behaviors. Hemicellulose undergoes a fluid stage and eventually forms millimetric spherical char particles. Cellulose does not become fully fluid but softens and forms millimetric char aggregates of different shapes. Lignin particles hardly soften and stick together in a curved slab. Comparison with model compounds allows us to infer that the degree of melting and the viscosity of the melt are responsible for the final particle shape. In fact, naphthalene pitch and glucose appear to be more viscous during pyrolysis and lead to the formation of three-dimensional columns a few millimeters high. Wax undergoes extensive melting, but the relatively low viscosity and the absence of crosslinking reactions eventually lead only to the formation of droplets. Full article
(This article belongs to the Special Issue Advances in Polymer Materials Based on Lignocellulosic Biomass)
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