Search Results (670)

The use of biostimulants and corroborants is increasing worldwide. Laboratory and field assays show their effectiveness in improving the vegetative performance of plants and their tolerance to abiotic stresses. This study aims to evaluate the in vitro activity of a biostimulant, based on pine bark extract, against some fungal phytopathogens. This research was carried out at the Laboratory of Plant Pathology (SAAF Department, University of Palermo, Italy), employing the poison food technique. Artificial agar media (Potato Dextrose Agar, PDA), simple or added with different concentrations of the biostimulant, were used to evaluate the differences in diametral growth of the fungi Aspergillus niger, Aspergillus tubingensis, Botrytis cinerea, Coriolopsis gallica, Fomitiporia mediterranea, Fusarium oxysporum, Pleurostoma richardsiae and Pleurotus ostreatus. The biostimulant was shown to contain the growth of most of the tested fungi, with the greatest effectiveness on A. tubingensis, C. gallica, F. mediterranea and P. richardsiae at the highest concentration, moderate effects on A. niger, F. oxysporum and P. ostreatus and no effect on B. cinerea. The observed fungistatic effects suggest that this biostimulant could contribute to integrated disease management while supporting more sustainable crop protection practices. In vivo tests aimed at evaluating the efficacy of these products on the evolution of different diseases in the field are ongoing, and preliminary results are promising but they are part of future work. Full article

Clustering techniques are widely applied to equity markets to uncover sectoral structures and regime shifts, yet most studies rely solely on empirical returns. This paper introduces a novel perspective by using diffusion-based parameters from the Black–Scholes model, namely monthly drift and diffusion, as clustering features. Using SET100 stocks in 2020, we applied k-means clustering and evaluated performances with silhouette scores, the Adjusted Rand Index, Wilcoxon tests, and an out-of-sample portfolio exercise. The results showed that diffusion-based features achieved higher silhouette scores in turbulent months, where they revealed sectoral divergence that log-returns failed to capture. The partition for November 2020 provided clearer sector separation and smaller portfolio losses, demonstrating predictive value beyond in-sample fit. Practically, the findings indicate that diffusion-based parameters can signal early signs of market stress, guide sector rotation decisions during volatile regimes, and enhance portfolio risk management by isolating persistent volatility structures across sectors. Theoretically, this model-based framework bridges equity clustering with stochastic diffusion representations used in derivatives valuation, offering a unified and interpretable tool for data-driven market monitoring. Full article

Healthcare providers in oncology experience exceptionally high stress rates. Research emphasizes that stress management programs must be quick to implement, flexible to accommodate demanding schedules, cost-effective, accessible to all staff, and tailored to the needs of oncology personnel. Programs that fail to meet these criteria often struggle with uptake and sustainability. This mixed-methods exploratory study aimed (1) to design an online stress management program, Breathe with the Waves (BWW), based on breathing techniques; (2) to evaluate its acceptability, satisfaction, and relevance; (3) to identify perceived benefits and challenges; and (4) to generate potential outcome measures for future studies. A team of Canadian researchers and end-users co-designed the intervention. Twenty oncology professionals completed BWW, which featured pre-recorded breathing videos, and provided feedback via questionnaires and semi-structured interviews. We used t-tests and Wilcoxon rank tests to analyze quantitative data, and template analysis for qualitative data. Participants found BWW highly acceptable, satisfactory, and relevant. Participants reported three categories of benefits: stress reduction, improved work performance, and increased mindfulness. Challenges included anticipated challenges and experienced challenges. Potential outcome measures fell into six categories: physical health, mental health, relational, work, mindfulness and personal practice. BWW, available in English and French, represents a promising and accessible approach to supporting the well-being of oncology personnel. Full article

Background/Objectives: One of the neurotoxic components from the sea trumpet polyps, Protopalythoa variabilis (Cnidaria, Anthozoa), is a 26-residue, V-shape helical peptide (PpVα). Its synthetic versions, i.e., the linear, the single-disulfide-bonded analog, and the chimeric peptide with a 6-residue stretch of the N-terminal native homologous peptide covalently linked to the linear sequence, were investigated for their activity on ion channels responsible for cellular excitability and synaptic transmission. Methods: Molecular docking analyses and dynamic simulations focused on the ability of PpVα peptides to bind ion channels selectively through interaction with critical residues at their binding sites. Results: Electrophysiological studies using the patch clamp technique with sympathetic bovine chromaffin cells from the adrenal medulla confirmed that PpVα analogs can block both sodium and calcium currents, which are responsible for initiating and propagating action potentials, respectively, and for neurotransmitter release. Additionally, the peptides displayed neuroprotective effects, attenuating cellular damage induced by veratridine, which interferes with sodium channel activity, and by oligomycin and rotenone (O/R), which affect mitochondrial function. Conclusions: The block of calcium and sodium channels and the neuroprotective effects against oxidative stress make the PpVα peptide scaffold an attractive template for developing agents that has significant clinical potential in several areas, such as the treatment of neurological diseases (epilepsy, multiple sclerosis, and neurodegenerative diseases), neuroprotection in acute events (stroke and traumatic brain or spinal cord injuries), the management of neuropathic pain, the prevention of ischemic damage, and psychiatric disorders (anxiety and bipolar disorder). Full article

Remote, self-managed neuroempowerment protocols are emerging as promising tools for promoting psychophysical well-being in healthy individuals through scalable, home-based interventions. Rooted in positive psychology, applied psychophysiology, and embodied awareness practices, these protocols increasingly leverage wearable technologies and digital platforms to support self-regulated training in cognitive, emotional, and physical domains. This scoping review explores the current literature on such interventions, guided by a triadic model of subjective well-being encompassing neurocognitive efficiency, psychological balance, and physical fitness. A systematic search across major scientific databases identified 28 studies meeting inclusion criteria, with a focus on home-based interventions targeting healthy adult populations using embodied awareness practices, applied psychophysiology techniques, and empowerment-based strategies. Findings indicate that these interventions yield improvements in attention regulation, stress reduction, and subjective well-being, particularly when combining digital feedback systems with embodied practices. However, significant methodological limitations persist, including the overreliance on self-report measures, lack of longitudinal follow-up, and insufficient integration of objective, multimodal assessment tools. Moreover, few studies explicitly address the role of participant engagement and agency—key elements in neuroempowerment frameworks that conceptualize the individual not as a passive recipient of treatment, but as an active agent in the training process. This review highlights the need for more rigorous and theoretically grounded research, advocating for integrative, adaptive intervention models supported by wearable neurotechnologies. Such approaches hold the potential to enhance motivation, personalize feedback, and promote sustainable well-being in ecologically valid, participant-centred ways. Full article

Tree-covered urban green spaces, including streets, parks, and other public areas, are vital for urban sustainability and people’s well-being. However, such trees face threats from the occurrence of extreme weather. In this study, we investigated wind damage to urban trees in the city of Debrecen, Hungary, during two severe windstorms in July 2025. Field surveys were conducted across three distinct urban zones, covering approximately 515,000 m² in total. We assessed 201 damaged and 325 undamaged trees and recorded the species, size, damage type, and contextual landscape features associated with them being damaged or not. Damage type to trees consisted primarily of broken branches, whilst uprooting and trunk breakage were recorded less often. Most tree characteristics (trunk circumference, height, systematic position, nativity) and the proximity and height of buildings upwind of focal trees were significant predictors of their vulnerability to windstorms. In addition, we surveyed 150 residents in person and received comments from 54 people via online questionnaires and explored their perceptions of storm frequency, the causes of storms, and mitigation measures. Most respondents noted increased storm frequency and attributed that to climate change, and they suggested mitigation measures focused on urban tree management and environmental protection. Some people expressed scepticism about the presence of climate change and/or their ability to address such damage on an individual basis. Our study is the first to integrate assessments of storm-related impacts on urban trees with the opinions of residents living in proximity to them. Our findings highlight the need for climate-adaptive and mechanically robust urban forestry planning and offer insights that guide the management of trees in urban areas globally. Specifically, we propose to undertake the following: (1) Prioritise structurally resilient, stress-tolerant tree species adapted to extreme weather conditions when planting new trees. (2) Integrate wind dynamics, microclimatic effects and artificial stabilisation techniques into urban design processes to optimise tree placement and their long-term stability. Urban planners, builders, developers, and homeowners should be informed about these stabilising practices and incorporate the needs of trees early in the design process, rather than as decorative additions. (3) Develop regionally calibrated risk models and early-warning systems to support proactive and data-driven tree management and public safety. (4) Promote climate literacy and public participation to strengthen collective stewardship and resilience of urban trees. Full article

Arc welding of ceramic materials remains a significantly underdeveloped research topic in materials science and manufacturing. This is primarily due to the difficulty in managing thermal gradients and stress concentrations during arc welding, which often result in cracking and poor joint reliability in ceramic components. However, recent advances suggest that gas tungsten arc welding and plasma arc welding could offer a promising pathway for ceramic joining if thoroughly investigated. While these techniques have proven effective in the precision welding of metals, their application to ceramics is met with several challenges, driven by the material’s inherent brittleness and high melting points. Consequently, further research is essential to understand the arc welding processes on ceramic materials and develop solutions to optimize and establish reliable welding procedures. Therefore, expanding this topic could have a significant impact on key engineering applications, where ceramic materials are increasingly in demand. Full article

Lung ischemia–reperfusion injury (LIRI) remains a major contributor to perioperative morbidity and mortality in thoracic surgery, especially for lung transplantations, where it is one of the principal drivers of primary graft dysfunction (PGD). Although substantial advances have been made in surgical technique, donor management, and perioperative care, LIRI continues to pose a significant clinical challenge. Mechanistically, LIRI reflects a combined pathology of oxidative stress, endothelial and glycocalyx disruption, innate immune activation, mitochondrial dysfunction, and regulated cell death, resulting in loss of alveolar–capillary barrier integrity and gas exchange failure. Current management is phase-specific and multimodal, spanning donor care and preservation, controlled reperfusion and lung-protective ventilation, and pharmacological treatments. Treatment candidates that target oxidative stress and inflammatory cascades (e.g., antioxidants, complement and adenosine pathways, mesenchymal stromal cell products, and dipeptidyl-peptidase-4 inhibition) show promise, yet translation into a clinical scenario remains difficult. Increasing evidence supports endothelial-preserving and mitochondria-sparing strategies, rigorous perioperative bundles, and biomarker-guided trials to move from pathophysiology to practice. Ultimately, addressing LIRI requires an integrated, multidisciplinary approach that spans surgical, anesthetic, and pharmacologic domains, with the goal of improving both early outcomes and long-term graft survival in lung transplant patients. Full article

Green spaces in subtropical cities are important for providing ecological services that support human well-being and serve as reservoirs for diverse microbial communities, which in turn support ecosystem functions. However, studies on the characteristics of the phyllosphere microbial community and public health risks associated with putative pathogens in various urban green spaces exposed to anthropogenic stress remain limited. To address this gap, we collected leaf samples from forests, greenbelts, parklands, and wetlands across Wuhan, China, and analyzed the bacterial and fungal communities via next-generation sequencing (NGS) techniques. For bacterial and fungal communities, alpha diversity was significantly greater in low-traffic zones than in high-traffic zones. Beta diversity analysis revealed distinct clustering of bacterial and fungal communities according to the urban green space type. Anthropogenic factors (foot traffic) influence green space type to shape microbial community structure, function, and stability, with shifts significantly associated with soil physicochemical properties via Mantel tests and redundancy analysis. The relative abundance of Enterobacter and Enterococcus was significantly greater in high-intensity parklands (HIPS) and high-intensity greenbelts (HIGS) (41.84, 38.32%), respectively. Our findings provide important information for the sustainable management of urban green spaces by regulating microbial communities, offering new insights into ecosystem health and human well-being. Full article

Efficiency in vitro regeneration is a crucial prerequisite for the application of New Nenomics Techniques (NGTs) in grapevine (Vitis vinifera L.) for improving resistance to biotic and abiotic stresses. This is especially true given that their management must be addressed sustainably, considering the impact of climate change. Unfortunately, in vitro plant regeneration and the establishment of embryogenic calluses are two genotype-dependent processes. Up to now, extensive research has been conducted on major international cultivars, whereas studies on the application of in vitro protocols for autochthonous cultivars remain limited. In this study, protocols for the acquisition of embryogenic calluses were applied on the most relevant Sicilian grapevine cultivars: the red-skinned ‘Frappato’, ‘Nerello mascalese’, and ‘Nero d’Avola’, and the white-skinned ‘Grillo’, ‘Carricante’, and ‘Catarratto’. Stamens and pistils were cultured in two different induction media (PIV and MSII) and at three stages (mother cells in the late premeiotic phase, tetrads, and mature pollen) to induce embryogenic calluses. Five thousand explants per cultivar were cultured, forming calluses in four selected cultivars. Plantlets were successfully generated from calluses of ‘Carricante’, ‘Frappato’, and ‘Nero d’Avola’. Moreover, protoplasts were isolated from ‘Frappato’ and ‘Nero d’Avola’. Our results establish a critical foundation for developing successful regeneration protocols for the future application of NGTs in Sicilian grapevine cultivars. Full article

Leaf water content (LWC) and chlorophyll content (CHL) are pivotal physiological indicators for assessing citrus growth and stress responses. However, conventional measurement techniques—such as fresh-to-dry weight ratio and spectrophotometry—are destructive, time-consuming, and limited in spatial and temporal resolution, making them unsuitable for large-scale monitoring. To achieve efficient large-scale monitoring, this study proposes a synergistic inversion framework integrating UAV multispectral remote sensing with intelligent optimization algorithms. Field experiments during the 2024 growing season (April–October) in western Hubei collected 263 ground measurements paired with multispectral images. Sensitive spectral bands and vegetation indices for LWC and CHL were identified through Pearson correlation analysis. Five modeling approaches—Partial Least Squares Regression (PLS); Extreme Learning Machine (ELM); and ELM optimized by Particle Swarm Optimization (PSO-ELM), Artificial Hummingbird Algorithm (AHA-ELM), and Grey Wolf Optimizer (GWO-ELM)—were evaluated. Results demonstrated that (1) VI-based models outperformed raw spectral band models; (2) the PSO-ELM synergistic inversion model using sensitive VIs achieved optimal accuracy (validation R²: 0.790 for LWC, 0.672 for CHL), surpassing PLS by 15.16% (LWC) and 53.78% (CHL), and standard ELM by 20.80% (LWC) and 25.84% (CHL), respectively; and (3) AHA-ELM and GWO-ELM also showed significant enhancements. This research provides a robust technical foundation for precision management of citrus orchards in drought-prone regions. Full article

Agroforestry systems offer clear environmental and agronomic advantages, but their effect on plant–biotic stressor interactions remains poorly understood. Specifically, the shade from companion trees can create microclimates favorable to fungal diseases on herbaceous crops. This potential drawback may offset other benefits, highlighting the urgent need for advanced plant health monitoring in these systems. This study assessed the potential of hyperspectral reflectance to detect the single and combined effects of simulated tree shading and infection by the fungal pathogen Alternaria alternata on grain sorghum (Sorghum bicolor L. Moench) under rainfed field conditions. Sorghum was grown either under full light or 50% shading conditions. Half of the plots were artificially inoculated with an A. alternata spore suspension (2 × 10⁸ CFU mL⁻¹), while the others served as controls. Leaf and ground-canopy measurements were acquired with a full range spectroradiometer (VNIR-SWIR, 400–2,400 nm) and UAV imagery covered the VIS-NIR range (400–1,000 nm) before the onset of visible symptoms. Permutational multivariate analysis of variance of leaf and ground-canopy data revealed significant effects of shading (Sh), infection (Aa), and their interaction (p < 0.05), allowing early detection of infection two days before symptom appearance, while UAV data showed only singular significant effects. Partial least squares discriminant analysis accuracy reached 78% at the leaf level, 90% at the ground-canopy level, and 74% (Sh) and 75% (Aa) at the UAV scale. Furthermore, vegetation spectral indices derived from the spectra confirmed greater physiological stress in shaded and infected plants, consistent with disease incidence assessments. Our results establish scale-specific hyperspectral reflectance spectroscopy as a powerful, non-destructive technique for early plant health surveillance in agroforestry. This advanced optical sensing capability is poised to illuminate complex stressor interactions, marking a significant step forward for precision agroforestry management. Full article

Seed priming has become a promising technique in agriculture and crop-stress management. Several authors have shown that the positive effects of seed priming are associated with various metabolic, physiological, and biochemical modifications (enzyme activation, membrane repair, initiation of DNA/RNA, and protein synthesis) that enhance the speed, uniformity, and vigor of germination. However, the mechanisms underlying seed priming are not yet well understood. The aim of our work was to study the quantitative and qualitative metabolic changes in the embryonic axes (radicle and plumule) and cotyledons of Vigna unguiculata (L.) Walp. Seeds were subjected to osmopriming with polyethylene glycol (PEG), simple hydropriming, and double hydropriming (a novel treatment). Results indicated that all types of priming, particularly double hydropriming, strongly stimulated the hydrolysis of protein and carbohydrate reserves. This resulted in a decrease in soluble proteins and starch contents and an increase in amino acids and soluble sugars contents. Moreover, the priming promoted the biosynthesis of osmolytes such as proline and induced qualitative changes in the composition of amino acids and soluble sugars. These biochemical changes depend on the organ and treatment method applied to the seeds. It is worth noting that double hydropriming induces metabolic modifications to a greater extent than single hydropriming. Full article

Background and Objectives: While transurethral resection of the prostate remains the gold standard for surgical treatment of benign prostatic hyperplasia, anatomical endoscopic enucleation of the prostate provides a safe, durable, and size-independent alternative. Our study introduces a specific technical innovation, i.e., en bloc bipolar prostate enucleation performed exclusively via sheath-tip mechanical dissection without the use of a dedicated enucleation loop, combined with the mushroom technique and early apical release. Materials and Methods: Between January 2018 and May 2023, 252 patients with prostate volumes > 30 mL and significant lower urinary tract symptoms underwent en bloc bipolar prostate enucleation via the mushroom technique with early apical release. Data were retrospectively evaluated to assess perioperative results, postoperative outcomes, and complications. Results: The median age of the cohort was 70 (65–76) years, with a median prostate volume of 60 (40–88.5) mL. The median operative time was 40 (30–70) min, and the median weight of enucleated tissue was 34 (16.5–60) g. Significant improvements were observed in the International Prostate Symptom score, Quality of Life score, maximum flow rate, average flow rate, and postvoid residual urine at 12 months (p < 0.001). The rate of transient stress urinary incontinence decreased from 19.44% at 1 month to 2.38% at 12 months. Conclusions: En bloc bipolar prostate enucleation using the mushroom technique is a safe and effective treatment for benign prostatic hyperplasia, yielding significant improvements in urinary symptoms and flow rates, with a manageable complication profile. Further multicenter studies are needed to confirm these findings. Full article

Background: Failure to rescue (FTR), defined as death after major postoperative complications, is a critical quality indicator in pancreatic cancer surgery. Despite advances in surgical techniques and perioperative care, FTR rates remain high and vary across institutions. Methods: This systematic review uses a narrative synthesis followed by PRISMA 2020. A PubMed search (1992–2025) identified 83 studies; after screening, 52 studies (2010–2025) were included. Eligible designs were registry-based, multicenter, single-center, or prospective audits. Given substantial heterogeneity in study designs, FTR definitions, and outcome measures, a narrative synthesis was performed; no formal risk-of-bias assessment or meta-analysis was conducted. Results: Definitions of FTR varied (in-hospital, 30-day, 90-day, severity-based, and complication-specific cases). Reported rates differed by definition: average reported rates were 13.2% for 90-day CD ≥ III (G1); 10.3% for in-hospital/30-day CD ≥ III (G3); and 7.4% for 30-day “serious/major” morbidity (G8). Absolute differences were +3.0 and +2.9 percentage points (exploratory, descriptive comparisons). Five domains were consistently associated with lower FTR: (i) centralization to high-volume centers; (ii) safe adoption/refinement of surgical techniques; (iii) optimized perioperative management including early imaging and structured escalation pathways; (iv) patient-level risk stratification and prehabilitation; and (v) non-technical skills (NTSs) such as decision-making, situational awareness, communication, teamwork, and leadership. Among NTS domains, stress and fatigue management were not addressed in any included study. Limitations: Evidence is predominantly observational with substantial heterogeneity in study designs and FTR definitions; the search was limited to PubMed; and no formal risk-of-bias, publication-bias assessment, or meta-analysis was performed. Consequently, estimates and associations are descriptive/associative with limited certainty and generalizability. Conclusions: NTSs were rarely used or measured across the included studies, with validated instruments; quantitative assessment was uncommon, and no study evaluated stress or fatigue management. Reducing the FTR after pancreatic surgery will require standardized, pancreas-specific definitions of FTR, process-level rescue metrics, and deliberate strengthening of NTS. We recommend a pancreas-specific operational definition with an explicit numerator/denominator: numerator = all-cause mortality within 90 days of surgery; denominator = patients who experience major complications (Clavien–Dindo grade III–V, often labeled “CD ≥ 3”). Addressing the gaps in stress and fatigue management and embedding behavioral metrics into quality improvement programs are critical next steps to reduce preventable mortality after complex pancreatic cancer procedures. Full article