Search Results (20)

Crop fidelity is a desirable trait for managed pollinators and is influenced by factors like competing forage sources and colony knowledge of the surrounding environment. In European honey bees (Apis mellifera L.), colonies deployed when the crop is flowering display the highest fidelity. We tested for a similar outcome using a stingless bee species that is being increasingly used as a managed pollinator in Australian macadamia orchards. We observed Tetragonula carbonaria (Smith) colonies deployed in macadamia orchards at three time points: (1) before crop flowering (“permanent”), (2) early flowering (“early”), and (3) later in the flowering period (“later”). We captured returning pollen foragers weekly and estimated crop fidelity from the proportion of macadamia pollen they collected, using light microscopy. Pollen foraging activity was also assessed via weekly hive entrance filming. The early and later introduced colonies initially exhibited high fidelity, collecting more macadamia pollen than the permanent colonies. In most cases, the permanent colonies were already collecting diverse pollen species from the local environment and took longer to shift over to macadamia. Pollen diversity increased over time in all colonies, which was associated with an increase in the proportion of pollen foragers. Our results indicate that stingless bees can initially prioritize a mass-flowering crop, even when flowering levels are low, but that they subsequently reduce fidelity over time. Our findings will inform pollinator management strategies to help growers maximize returns from pollinator-dependent crops like macadamia. Full article

Stingless bees are vital pollinators in tropical and neotropical regions, emphasizing the need to conserve these species. However, resource scarcity, particularly pollen, during certain periods negatively impacts bee health and pollination efficiency. To address this, we developed a fermented protein feed using microorganisms from pollen of Melipona quadrifasciata, a species commonly found in the Brazilian Atlantic Forest. The fermented feed consisted of a protein bran mixture, sugar syrup, and an inoculant derived from species’ fermented pollen. To assess the feed quality, nutritional evaluation and metabolomic analyses were conducted (UHPLC/DAD-HRMS/MS and GC-MS). The metabolomic analysis identified 85 compounds in positive and 102 in negative ionization modes. Shared metabolites, such as isoquercetin and palatinose, highlighted similarities between the feed and natural fermented pollen. Laboratory tests with caged bees showed comparable consumption between the feed and fermented pollen with no statistical difference (mean: 0.067 g vs. 0.060 g; p = 0.53). The feed exhibited a favorable pH transition (5.30 pre-fermentation to 4.41 after the fermentation process) and remained stable for six months at ±2 °C. These findings demonstrate a biotechnological advance in stingless bee nutrition, offering a viable solution during resource scarcity and promoting the health of M. quadrifasciata. Full article

Stingless bees (Hymenoptera; Apidae; Meliponini), with a biodiversity of 605 species, harvest and transport corbicula pollen to the nest, like Apis mellifera, but process and store the pollen in cerumen pots instead of beeswax combs. Therefore, the meliponine pollen processed in the nest was named pot-pollen instead of bee bread. Pot-pollen has nutraceutical properties for bees and humans; it is a natural medicinal food supplement with applications in health, food science, and technology, and pharmaceutical developments are promising. Demonstrated synergism between Tetragonisca angustula pot-pollen ethanolic extracts, and antibiotics against extensively drug-resistant (XDR) bacteria revealed potential to combat antimicrobial resistance (AMR). Reviewed pot-pollen VOC richness was compared between Australian Austroplebeia australis (27), Tetragonula carbonaria (31), and Tetragonula hogkingsi (28), as well as the Venezuelan Tetragonisca angustula (95). Bioactivity and olfactory attributes of the most abundant VOCs were revisited. Bibliometric analyses with the Scopus database were planned for two unrelated topics in the literature for potential scientific advances. The top ten most prolific authors, institutions, countries, funding sponsors, and sources engaged to disseminate original research and reviews on pot-pollen (2014–2023) and direct injection food flavor (1976–2023) were ranked. Selected metrics and plots were visualized using the Bibliometrix-R package. A scholarly approach gained scientific insight into the interaction between an ancient fermented medicinal pot-pollen and a powerful bioanalytical technique for fermented products, which should attract interest from research teams for joint projects on direct injection in pot-pollen flavor, and proposals on stingless bee nest materials. Novel anti-antimicrobial-resistant agents and synergism with conventional antibiotics can fill the gap in the emerging potential to overcome antimicrobial resistance. Full article

Honey and pollen from Tetragonisca fiebrigi and Scaptotrigona jujuyensis, stingless bees from northern Argentina, presented a particular microbiological profile and associated enzymatic activities. The cultured bacteria were mostly Bacillus spp. (44%) and Escherichia spp. (31%). The phylogenetic analysis showed a taxonomic distribution according to the type of bee that was similar in both species. Microbial enzymatic activities were studied using hierarchical clustering. Bacillus spp. was the main bacterium responsible for enzyme production. Isolates with xylanolytic activity mostly presented cellulolytic activity and, in fewer cases, lipolytic activity. Amylolytic activity was associated with proteolytic activity. None of the isolated strains produced multiple hydrolytic enzymes in substantial amounts, and bacteria were classified according to their primary hydrolytic activity. These findings add to the limited knowledge of microbiological diversity in honey and pollen from stingless bees and also provide a physiological perspective of this community to assess its biotechnological potential in the food industry. Full article

(1) Background: The identification of microorganisms includes traditional biochemical methods, molecular biology methods evaluating the conserved regions of rRNA, and the molecular biology of proteins (proteomics), such as MALDI-TOF MS mass spectrometry. This work aimed to identify the biodiversity of yeasts associated with stingless bee species’ honey and pollen, Melipona scutellaris, Nannotrigona testaceicornes, and Tetragonisca angustula, from the region of São Gonçalo dos Campos-Bahia (BA) state, Brazil. (2) Methods: Cellular proteins were extracted from 2837 microbial isolates (pollen and honey) and identified via MALDI-TOF MS. The identified yeast species were also compared to the mass spectra of taxonomically well-characterized reference strains, available from the National Center of Biotechnology Information (NCBI) database. (3) Results: Nine yeast species were identified: Candida maltosa, Candida norvegica, Kazachstania telluris, Schizosaccharomyces pombe, Scheffersomyces insectosus, Meyerozyma guilliermondii, Brettanomyces bruxellensis, Kazachstania exigua, and Starmerella lactis-condensi. Nannotrigona testaceicornes pollen had the highest number of yeast colonies. The yeasts Brettanomyces bruxellensis and Kazachstania telluris showed high populations in the samples of Nannotrigona testaceicornes and Melipona scutellaris, respectively. This work shows that there is some sharing of the same species of yeast between honey and pollen from the same beehive. (4) Conclusions: A total of 71.84% of the identified species present a high level of confidence at the species level. Eight yeast species (Candida maltosa, Candida norvegica, Kazachstania telluris, Schizosaccharomyces pombe, Scheffersomyces insectosus, Meyerozyma guilliermondii, Kazachstania exigua, and Starmerella lactis-condensi) were found for the first time in the samples that the authors inspected. This contributes to the construction of new knowledge about the diversity of yeasts associated with stingless bee products, as well as to the possibility of the biotechnological application of some yeast species. Full article

Rosenbergiella bacteria have been previously isolated predominantly from floral nectar and identified in metagenomic screenings as associated with bees. Here, we isolated three Rosenbergiella strains from the robust Australian stingless bee Tetragonula carbonaria sharing over 99.4% sequence similarity with Rosenbergiella strains isolated from floral nectar. The three Rosenbergiella strains (D21B, D08K, D15G) from T. carbonaria exhibited near-identical 16S rDNA. The genome of strain D21B was sequenced; its draft genome contains 3,294,717 bp, with a GC content of 47.38%. Genome annotation revealed 3236 protein-coding genes. The genome of D21B differs sufficiently from the closest related strain, Rosenbergiella epipactidis 2.1A, to constitute a new species. In contrast to R. epipactidis 2.1A, strain D21B produces the volatile 2-phenylethanol. The D21B genome contains a polyketide/non-ribosomal peptide gene cluster not present in any other Rosenbergiella draft genomes. Moreover, the Rosenbergiella strains isolated from T. carbonaria grew in a minimal medium without thiamine, but R. epipactidis 2.1A was thiamine-dependent. Strain D21B was named R. meliponini D21B, reflecting its origin from stingless bees. Rosenbergiella strains may contribute to the fitness of T. carbonaria. Full article

The management of natural resources based on socio-economic and ecology development has led to a focus on the bioeconomy in the policy discourse of non-timber forest products (NTFPs). Honey is an important NTFP with high socio-economic value, and its production involves millions of Indonesians. This article reviews the current status of honey-producing bee management, cultivation and harvesting system, marketing and socio-economic values, and the industry’s environmental function in Indonesia. This research utilized a meta-narrative review method to collect data and information from Google Scholar, Scopus, Science Direct, ResearchGate, Sinta, and Garuda. The study showed that the four bee species, namely Apis mellifera, Apis cerana, Apis dorsata, and stingless bee, are the most common species in honey production in Indonesia. The four species have specific characteristics based on habitat, production capacity, derivative products, management intervention to meet honey product standards, and sustainable livelihoods. The value chain of bees’ major products, such as honey, propolis, pollen, royal jelly, wax, and other derivative products, involves the distribution of honey to all involved communities, including beekeepers, honey gatherers/hunters, intermediate traders, and the processing industry. This study also found a significant association between environmental sustainability statutes that affects functional sustainability and economic function. The finding parallels the global trends that put forward a forest-based bioeconomy approach to forest resource management. The policy must be strengthened in managing relationships among supporting actors for sustainable honey production. Full article

With technological advancements in the medicinal and pharmaceutical industries, numerous research studies have focused on the propolis produced by stingless bees (Meliponini tribe) and Apis mellifera honeybees as alternative complementary medicines for the potential treatment of various acute and chronic diseases. Propolis can be found in tropical and subtropical forests throughout the world. The composition of phytochemical constituents in propolis varies depending on the bee species, geographical location, botanical source, and environmental conditions. Typically, propolis contains lipid, beeswax, essential oils, pollen, and organic components. The latter include flavonoids, phenolic compounds, polyphenols, terpenes, terpenoids, coumarins, steroids, amino acids, and aromatic acids. The biologically active constituents of propolis, which include countless organic compounds such as artepillin C, caffeic acid, caffeic acid phenethyl ester, apigenin, chrysin, galangin, kaempferol, luteolin, genistein, naringin, pinocembrin, coumaric acid, and quercetin, have a broad spectrum of biological and therapeutic properties such as antidiabetic, anti-inflammatory, antioxidant, anticancer, rheumatoid arthritis, chronic obstruct pulmonary disorders, cardiovascular diseases, respiratory tract-related diseases, gastrointestinal disorders, as well as neuroprotective, immunomodulatory, and immuno-inflammatory agents. Therefore, this review aims to provide a summary of recent studies on the role of propolis, its constituents, its biologically active compounds, and their efficacy in the medicinal and pharmaceutical treatment of chronic diseases. Full article

The stingless bee has been gaining more attention in recent years due to the uniqueness and benefits of its products. Similar to the common honeybee, stingless bees also produce honey, propolis, and pollen, which offer superior benefits for direct or indirect consumption. However, reports on the benefits of stingless bee products are scarce. This article summarises recent reports on stingless bee products. The function and application of the properties of the products such as phenolic compounds, antioxidant properties, and chemical content are elucidated. The antimicrobial properties and anticancer potential of the products are also highlighted. Future trends, potential, and uniqueness of stingless bee products are discussed. Stingless bee honey is highlighted as a superfood that exceptionally has the potential to be an active ingredient in treating cancer. Stingless bee propolis has been extensively studied for its rich beneficial chemical compounds that contribute to its antioxidant properties. Though studies on stingless bee pollen are scarce, it has been reported that it also has the potential of being a functional food. Full article

There is a high diversity of bees in the tropics, including honey bees and stingless bees, which are the main sources for honey and other ecosystem services. In Indonesia, beekeeping practices have been developed for centuries, and they have been part of many cultural practices in many traditional communities. The objective of this research was to study the beekeeping status and managed bee diversity in Indonesia and to investigate beekeepers’ perspectives on the factors and obstacles related to beekeeping. Direct interview and online interview were conducted to gain data on bees and beekeepers. In total, 272 beekeepers were interviewed across 25 provinces. Samplings of honey bees and stingless bees were also done during direct interviews for further identification and, when possible, pollen identification. All data and specimens were then sent to IPB Bogor for compilation and identification. We recorded 22 species of bees, including 3 species of honey bees and 19 species of stingless bees, that are reared by Indonesian beekeepers, with Apis cerana and Tetragonula laeviceps as the most common species. Our research also found that the majority of beekeepers fall into the category of the younger generation (30–39 years old) with educational background mostly from senior high school. Based on the beekeepers’ perspectives, there are several obstacles to beekeeping, especially the occurrence of death of bee foragers attributed to climate, food source, and pesticides. In conclusion, there is a need to develop a strategy for beekeeping and bee conservation in Indonesia, especially for adaptation and mitigation from environmental changes with a particular focus on climate and land-use change. Full article

Although the quality of citizen science (CS) data is often a concern, evidence for high-quality CS data increases in the scientific literature. This study aimed to assess the data reliability of a structured CS protocol for monitoring stingless bees’ flight activity. We tested (1) data accuracy for replication among volunteers and for expert validation and (2) precision, comparing dispersion between citizen scientists and expert data. Two distinct activity dimensions were considered: (a) perception of flight activity and (b) flight activity counts (entrances, exits, and pollen load). No significant differences were found among groups regarding entrances and exits. However, replicator citizen scientists presented a higher chance of perceiving pollen than original data collectors and experts, likely a false positive. For those videos in which there was an agreement about pollen presence, the effective pollen counts were similar (with higher dispersion for citizen scientists), indicating the reliability of CS-collected data. The quality of the videos, a potential source of variance, did not influence the results. Increasing practical training could be an alternative to improve pollen data quality. Our study shows that CS provides reliable data for monitoring bee activity and highlights the relevance of a multi-dimensional approach for assessing CS data quality. Full article

Flower visiting stingless bees store collected pollen and nectar for times of scarcity. This stored food is of high value for the colony and should be protected against con- and heterospecifics that might rob them. There should be high selective pressure on the evolution of mechanisms to discriminate nestmates from non-nestmates and to defend the nest, i.e., resources against intruders. Multimodal communication systems, i.e., a communication system that includes more than one sensory modality and provide redundant information, should be more reliable than unimodal systems. Besides olfactory signals, vibrational signals could be used to alert nestmates. This study tests the hypothesis that the vibrational communication mode plays a role in nest defense and nestmate recognition of Axestotrigona ferruginea. Substrate vibrations induced by bees were measured at different positions of the nest. The experiments show that guarding vibrations produced in the entrance differ in their temporal structure from foraging vibrations produced inside the nest. We show that guarding vibrations are produced during non-nestmate encounters rather than nestmate encounters. This further supports the idea that guarding vibrations are a component of nest defense and alarm communication. We discuss to whom the vibrations are addressed, and what their message and meaning are. Full article

Stingless bee-collected pollen (bee bread) is a mixture of bee pollen, bee salivary enzymes, and regurgitated honey, fermented by indigenous microbes during storage in the cerumen pot. Current literature data for bee bread is overshadowed by bee pollen, particularly of honeybee Apis. In regions such as South America, Australia, and Southeast Asia, information on stingless bee bee bread is mainly sought to promote the meliponiculture industry for socioeconomic development. This review aims to highlight the physicochemical properties and health benefits of bee bread from the stingless bee. In addition, it describes the current progress on identification of beneficial microbes associated with bee bread and its relation to the bee gut. This review provides the basis for promoting research on stingless bee bee bread, its nutrients, and microbes for application in the food and pharmaceutical industries. Full article

Bee products have been used since ancient times both for their nutritional value and for a broad spectrum of therapeutic purposes. They are deemed to be a potential source of natural antioxidants that can counteract the effects of oxidative stress underlying the pathogenesis of many diseases. In view of the growing interest in using bioactive substances from natural sources to promote health and reduce the risk of developing certain illnesses, this review aims to update the current state of knowledge on the antioxidant capacity of bee products such as honey, pollen, propolis, beeswax, royal jelly and bee venom, and on the analytical methods used. The complex, variable composition of these products and the multitude of analytical methods used to study their antioxidant activities are responsible for the wide range of results reported by a plethora of available studies. This suggests the need to establish standardized methods to more efficiently evaluate the intrinsic antioxidant characteristics of these products and make the data obtained more comparable. Full article

Stingless bees are a type of honey producers that commonly live in tropical countries. Their use for honey is being abandoned due to its limited production. However, the recent improvements in stingless bee honey production, particularly in South East Asia, have brought stingless bee products back into the picture. Although there are many stingless bee species that produce a wide spread of products, known since old eras in traditional medicine, the modern medical community is still missing more investigational studies on stingless bee products. Whereas comprehensive studies in the current era attest to the biological and medicinal properties of honeybee (Apis mellifera) products, the properties of stingless bee products are less known. This review highlights for the first time the medicinal benefits of stingless bee products (honey, propolis, pollen and cerumen), recent investigations and promising future directions. This review emphasizes the potential antioxidant properties of these products that in turn play a vital role in preventing and treating diseases associated with oxidative stress, microbial infections and inflammatory disorders. Summarizing all these data and insights in one manuscript may increase the commercial value of stingless bee products as a food ingredient. This review will also highlight the utility of stingless bee products in the context of medicinal and therapeutic properties, some of which are yet to be discovered. Full article