Search Results (1,130)

The increasing prevalence of the mobile tigecycline resistance gene tet(X) poses a severe global health threat, and the genus Acinetobacter is a major reservoir. This study aimed to develop a rapid and specific multiplex PCR assay for detecting the predominant tet(X)-positive Acinetobacter species. Through pan-genome analyses of 390 tet(X)-positive Acinetobacter genomes, a total of 20 tet(X) variants were identified in 24 Acinetobacter species, including 17 published lineages and seven taxonomically unresolved Taxa. Acinetobacter indicus (30.8%), Acinetobacter amyesii (17.2%), and Acinetobacter towneri (16.1%) were the top three hosts of diverse tet(X) variants. Species-specific signature genes were identified and used for primer design, yielding amplicons of 267 bp (tet(X)), 424 bp (A. indicus), 690 bp (A. amyesii), and 990 bp (A. towneri). The assay was rigorously adjusted for an optimal annealing temperature of 52.8 °C and a primer ratio of 1:1:1:1, demonstrating high sensitivity with a detection limit of 0.3 ng/μL DNA and excellent stability under −20 °C, 4 °C, 20 °C storage conditions. Validation experiments on 151 bacterial strains showed high accuracy for DNA templates (≥97.8%) and bacterial suspensions (≥93.5%) within two hours. This cost-effective and highly accurate multiplex PCR provides a powerful tool for proactive surveillance and control of the critical Acinetobacter sp. pathogens. Full article

The development of rare-earth-free permanent magnets represents a strategic direction in advanced magnetic materials research. Among the most promising candidates, the metastable α″-Fe₁₆N₂ phase stands out due to its exceptionally high saturation magnetization. In this work, α″-Fe₁₆N₂ powders produced by gas atomization followed by nitriding were consolidated via Spark Plasma Sintering (SPS). The effects of sintering temperature (498–598 K) and pressure (40–80 MPa) on phase evolution, densification, microstructure, and magnetic properties have been systematically investigated. Optimal processing conditions were identified at 548 K and 60 MPa, providing a balance between densification (~80% of the theoretical density), phase stability, and magnetic performance. X-ray diffraction revealed that the α″-Fe₁₆N₂ phase remains stable up to ~523 K, while its decomposition into α-Fe and γ′-Fe₄N becomes significant at higher temperatures. The consolidated samples exhibited a saturation magnetization of ~230 Am²/kg, a maximum coercivity of ~86.5 kA/m, and a Mr/Ms ratio of 0.42. δM curve analysis indicated a transition from magnetostatic interactions (at low pressures) to exchange-dominated coupling (at intermediate and high pressures). These findings demonstrate the potential of SPS processing to preserve the α″-Fe₁₆N₂ phase and produce rare-earth-free magnetic compacts with competitive magnetic performance, providing a basis for further process optimization. Full article

We introduce the Reward–VaR curve, a novel framework for evaluating risk-adjusted investment performance across a range of investor risk preferences. When returns are normally distributed, the Reward–VaR curve yields the same asset ranking as the Sharpe ratio. However, when the third-order modified VaR is used, a new paradigm emerges beyond the simplistic “better/worse” ranking: if no asset dominates at all confidence levels, one becomes preferable for risk-averse investors, while the other is favored by the risk-tolerant. For empirical implementation, we incorporate bootstrapping to separate robust performance patterns from sampling noise. We apply the methodology to compare conventional equity indices and their Islamic counterparts from the S&P Dow Jones Global Index family across nine markets from 2000 to 2024: Asia-Pacific, Canada, Developed, Emerging, Europe, Japan, UK, US, and World. Our empirical results reveal market-condition dependent dominance patterns. During bull markets, conventional indices dominate in most regions, except the European and World markets, where no dominance is observed, and Japan, where the Islamic index outperforms. In bear markets, Islamic indices dominate in most regions, with the exception of Emerging Markets, where dominance is partial, and Japan, where no clear difference is observed. Over the full sample, most markets show no significant long-run dominance, except Canada and Emerging Markets, where conventional indices outperform. Full article

Yttrium oxide (Y₂O₃) is a crucial protective material for the inner walls of semiconductor etching chambers. This study employed Suspension Plasma Spray (SPS) technology to deposit Y₂O₃ coatings on AISI 304 stainless steel substrates. A water ring guide cover, which injects deionized water toward the center of the plasma flame at the torch outlet, was installed. The critical parameter ratio between the water ring flow rate and the suspension feed rate was investigated, with a specific focus on its influence on the coating’s microstructure and mechanical properties. The findings reveal that this parameter exhibits a significant positive correlation with porosity, with the coefficient of determination R² for their linear fit reaching 0.91236. When the water ring flow rate ratio was reduced to 79.66%, the porosity decreased to 0.946%, while the primary composition of the coating remained unchanged. Bond strength tests demonstrated that the adhesion strength of the coating exhibits an upward trend with increasing proportion of water ring flow. The adhesion strength reached its maximum value of 27.02 MPa when the water ring flow rate proportion was increased to 85.45%. Roughness exhibits a non-monotonic variation trend within the ratio range, attaining its optimal minimum value at the lower end of the ratio, indicating complex interrelationships among process characteristics. This work concludes that a low water ring flow rate ratio is essential for fabricating dense, well-adhered, and smooth Y₂O₃ coatings via SPS, providing a critical guideline for process optimization for applications such as semiconductor protection. Full article

Most reinforcement learning (RL) methods for portfolio optimization remain limited to single markets and a single algorithmic paradigm, which restricts their adaptability to regime shifts and heterogeneous conditions. This paper introduces a generalized version of the Modular Portfolio Learning System (MPLS), extending beyond its initial PPO backbone to integrate four RL algorithms: Proximal Policy Optimization (PPO), Deep Q-Network (DQN), Deep Deterministic Policy Gradient (DDPG), and Soft Actor-Critic (SAC). Building on its modular design, MPLS leverages specialized components for sentiment analysis, volatility forecasting, and structural dependency modeling, whose signals are fused within an attention-based decision framework. Unlike prior approaches, MPLS is evaluated independently on three major equity indices (S&P 500, DAX 30, and FTSE 100) across diverse regimes including stable, crisis, recovery, and sideways phases. Experimental results show that MPLS consistently achieved higher Sharpe ratios—typically +40–70% over Minimum Variance Portfolio (MVP) and Risk Parity (RP)—while limiting drawdowns and Conditional Value-at-Risk (CVaR) during stress periods such as the COVID-19 crash. Turnover levels remained moderate, confirming cost-awareness. Ablation and variance analyses highlight the distinct contribution of each module and the robustness of the framework. Overall, MPLS represents a modular, resilient, and practically relevant framework for risk-aware portfolio optimization. Full article

In this work, the results of the structural and phase state of LaNi₅-based alloys modified with Ti, Mn, and Co elements, obtained by mechanical alloying and subsequent spark plasma sintering, are presented. X-ray diffraction analysis was carried out to determine the phase composition, lattice parameters, microstrain, and average crystallite size, as well as to study the morphology and microstructure of the synthesized samples. It was established that the ball-to-powder ratio (BPR) and the milling speed affect the degree of intermetallic phase formation and the level of accumulated microstrains. The optimal mechanical alloying parameters make it possible to form the necessary precursor components for subsequent spark plasma sintering (SPS). It was determined that the SPS process effectively promotes the formation of intermetallic phases such as TiNi, LaNi₄Mn, LaNi₃Mn₂, and LaNi₄Co, ensuring high crystallinity and a reduction in defects accumulated during mechanical alloying. The morphology and microstructure of the samples with titanium, manganese, and cobalt additions showed that at the mechanical alloying stage, all systems are characterized by a dispersed and agglomerated structure, a wide particle size distribution, and a developed surface. After SPS, all series exhibited material consolidation and the formation of a dense matrix with distinct grain boundaries. Full article

This research work presents a techno-economic assessment of biodiesel production with non-standard waste cooking oil (WCO) (brown grease of small restaurants, yellow grease of households) and semi-open Chlorella sp. microalgal cultivation, which covers the problematic areas of scale and cost-efficiency in sustainable biodiesel production. Cost-effective biodiesel feedstock research has been motivated by the urgency of finding sustainable sources of energy. With base-catalyzed transesterification optimized by ANOVA and response surface methodology (RSM), the present study recorded biodiesel yields of up to 99.08% in household WCO (at optimum conditions; 55 °C, 3.3 mg/g NaOH, ethanol) and 96.61% in restaurant WCO (at optimum conditions; 54 °C, 1.5 mg/g NaOH, methanol) compared to 28.6% in Chlorella sp. (semi-open photobioreactors). Concerning the two types of WCO feedstocks, the obtained equations are able to compute the biodiesel viscosity and yield, in good correlation with the experimental values, in relation to the temperature and ratio of catalyst to oil/alcohol solution. The assessed household WCO has better yield and quality as it contains fewer impurities, whereas the restaurant WCO needed to be further purified, driving up the prices. Although Chlorella biodiesel is carbon neutral, its production and extraction costs are higher, making it less economically feasible for biodiesel production. Economic analysis showed that the capital costs of household WCO, restaurant WCO, and Chlorella sp. are USD 190,000, USD 220,000, and USD 720,000, respectively, based on 1,000,000 L/year as biodiesel production rate. Low capital costs as well as byproduct glycerol income of the two investigated types of WCO play a role in their low payback periods (0.23–0.91 years) and high ROI (110–444.4%). The analysis highlights the economic and environmental benefits of WCO, especially household WCO, as a scalable biodiesel feedstock, which provides new insights into process optimization and sustainable biodiesel strategies. To enhance its sustainability and cost-effectiveness and contribute to the transition to renewable biofuels globally, future studies need to emphasize energy reduction in microalgae production and purification of restaurant WCO. Full article

Senecavirus A (SVA) causes a vesicular disease in pigs with clinical signs indistinguishable from those of other swine vesicular diseases. To enable serological differentiation infected from vaccinated animals (DIVA), we developed indirect ELISAs (iELISAs) based on recombinant non-structural proteins (NSPs). A His-tagged tandem antigen, r3AB-3C, was designed by integrating immunodominant B-cell epitopes from 3AB and 3C proteins, and was successfully expressed in Escherichia coli (E. coli) and purified alongside the individual r3AB and r3C proteins. Serological evaluation results showed that the immunoreactivity of the r3AB-3C iELISA was superior to that of r3AB, which in turn was better than r3C. The r3AB-3C and r3AB iELISAs were subsequently validated. The cut-off values were established at sample-to-positive (S/P) ratios of ≥0.2635 for the r3AB-3C iELISA and ≥0.5775 for the r3AB iELISA. The r3AB-3C iELISA demonstrated higher sensitivity for detecting infection-induced antibodies than the r3AB iELISA, despite the later seroconversion of anti-NSP antibodies compared to neutralizing antibodies. In a serosurvey, the r3AB-3C iELISA revealed seropositivity rates of 35.2% in 2023 and 22.3% in 2024. In conclusion, the r3AB-3C iELISA is a valuable serological tool for monitoring SVA infection, effectively supporting DIVA strategies. Full article

Severe leaf galling by the jujube gall midge Dasineura jujubifolia (Diptera: Cecidomyiidae) compromises photosynthesis and yield in arid-zone jujube orchards, yet Xinjiang-specific evidence to guide biological control has been scarce. Here we provide the first systematic characterization in Xinjiang (Hami, China) of D. jujubifolia and its parasitoid complex, integrating region-specific field surveys with gall dissection and laboratory assays. We documented five parasitoid wasps, including two species newly recorded in China—Pseudotorymus samsatensis (Hymenoptera: Torymidae) and Baryscapus adalia (Hymenoptera: Eulophidae). In Hami, the host completed 4–5 generations per year with a 19–24-day generation time. Functional roles were partitioned: P. samsatensis (dominant), Systasis parvula (Hymenoptera: Pteromalidae), and B. adalia were larval ectoparasitoids, whereas Aprostocetus sp. (Hymenoptera: Eulophidae) and Synopeas sp. (Hymenoptera: Platygastridae) were endoparasitoids. Time-series data revealed tight temporal synchrony between P. samsatensis and host peaks. Controlled experiments quantified daily emergence rhythms, diet-dependent adult longevity, and sex ratios, providing parameters to inform release timing and conservation in biological control programs. Collectively, these findings establish management-ready baselines for D. jujubifolia and its parasitoids in arid jujube systems and support conservation-oriented, reduced-pesticide integrated pest management (IPM). Full article

Background: Oxidative stress represents a key pathogenic factor in spondyloarthritis (SpA), yet its comprehensive assessment remains underutilized in routine clinical practice. Objectives: We evaluated oxidative stress biomarker profiles in SpA patients to determine associations with disease activity, systemic inflammation, structural damage, lifestyle factors, and therapeutic responses for practical clinical implementation. Methods: This cross-sectional study included 101 patients meeting the Assessment of SpondyloArthritis International Society (ASAS) 2009 criteria. Oxidative stress assessment utilized a validated biomarker panel: copper, zinc, glutathione peroxidase (GPx), ceruloplasmin (Cp), transferrin (TF), haptoglobin (Hp), bilirubin (BR), and uric acid (UA). Clinical, radiological, lifestyle, and therapeutic data underwent systematic analysis. Results: Glutathione peroxidase activity was elevated in 82.1% of patients, establishing it as the most sensitive oxidative stress marker. Copper levels increased in 30.7% and zinc deficiency occurred in 36.4% of cases. Oxidative stress markers correlated significantly with inflammatory parameters (erythrocyte sedimentation rate [ESR], C-reactive protein [CRP], neutrophil-to-lymphocyte ratio [NLR], platelet-to-lymphocyte ratio [PLR], neutrophil-to-monocyte ratio [NMR], systemic immune-inflammation index [SII]) and disease activity scores (Bath Ankylosing Spondylitis Disease Activity Index [BASDAI], Ankylosing Spondylitis Disease Activity Score based on CRP [ASDAS-CRP], Disease Activity Score 44 [DAS44-CRP]). Higher oxidative stress was associated with a poorer quality of life, as indicated by elevated Ankylosing Spondylitis Quality of Life (ASQoL) scores. Physical activity and adherence to a Mediterranean diet were independently associated with better antioxidant capacity. Smoking and nonsteroidal anti-inflammatory drug (NSAID) use correlated with increased oxidative burden. Anti-tumor necrosis factor alpha (anti-TNFα) therapy was associated with reduced levels of oxidative stress. Structural damage, particularly cervical spine involvement, correlated with heightened oxidative stress. Conclusions: This comprehensive evaluation reveals significant clinical correlations between oxidative stress and multiple disease domains in SpA. Modifiable lifestyle factors and therapeutic interventions have a significant impact on the redox balance. These findings establish practical targets for personalized management. The integration of oxidative stress assessment into routine practice could enhance disease monitoring and inform the development of antioxidant-based therapeutic strategies. Full article

This study examines whether CEO risk tolerance influences a firm’s sustainable practices, as measured by Environmental, Social, and Governance (ESG) scores. The analysis uses facial width–height ratio (fWHR) as a proxy for CEO testosterone and risk-taking behavior. A regression analysis of S&P 500 firms from 2018 to 2022 shows that a greater fWHR is negatively associated with ESG scores, although the economic effect is small. A one standard deviation increase in fWHR decreases ESG by half a point on a 100-point scale. Further investigation into CEO turnover reveals a surprising asymmetry: when a new CEO has a higher fWHR, ESG scores increase significantly compared to firms without a CEO change. This finding, along with other confounding effects, suggests that a certain amount of calculated, strategic risk-taking may be necessary to successfully promote corporate sustainability programs. Full article

This paper presents the first rigorous empirical investigation into a fundamental question of cryptocurrency valuation: Are cryptocurrency prices in line with the prices of fundamental assets? To answer this, we analyze the nine largest cryptocurrencies by market capitalization—Bitcoin (BTC), Ethereum (ETH), Solana (SOL), Binance Coin (BNB), Ripple (XRP), Cardano (ADA), Litecoin (LTC), Tron (TRX), and the stablecoin DAI—against a suite of traditional benchmarks, including major fiat currencies (EUR, CAD, JPY), gold, and the S&P500 index. Our dataset spans from 1 January 2014 to 30 June 2025, with start dates varying for newer cryptocurrencies to ensure robust time series analysis. Guided by the asset pricing theory, we formulate a martingale test: if a cryptocurrency is priced in line with a fundamental numeraire asset, its price ratio relative to that numeraire must follow a martingale process. Our extensive empirical analysis reveals that the prices of major cryptocurrencies (BTC, ETH, SOL, BNB) consistently reject the martingale hypothesis when traditional assets (currencies, gold, equities) serve as the numeraire, indicating a decoupling from fundamental valuation anchors. Conversely, when Bitcoin or Ethereum itself is used as the numeraire, most smaller cryptocurrencies are priced in line with these crypto benchmarks, suggesting an internal valuation ecosystem that operates independently of traditional finance. Full article

Aging-associated skeletal muscle and intestinal dysfunction is largely driven by chronic inflammation, oxidative stress, and microbiota imbalance. This study investigated the protective effects of a lychee fermentate (LF) in a D-galactose-induced aging mouse model. LF was prepared using a mixed microbial fermentation approach with Lactiplantibacillus plantarum, Lacticaseibacillus casei, Saccharomyces cerevisiae, and Acetobacter pasteurianus SP021. LF administration significantly improved muscle strength and endurance and restored muscle fiber morphology. Meanwhile, LF alleviated colonic transit impairment and downregulated senescence markers p16 and p21. H&E and AB-PAS staining showed that intervention with LF ameliorated the colonic tissue damage, preserved goblet cell populations and promoted MUC2-mediated mucus secretion, which was further confirmed by the upregulation of intestinal barrier-related proteins MUC2, ZO-1, Claudin-1, and Occludin through immunofluorescence analysis. In addition, LF reduced colonic inflammation by suppressing IL-1β, IL-6, TNF-α, CXCL1, and MCP1 expression, and mitigated oxidative stress by lowering malondialdehyde levels to 24.65 ± 3.84 nmol/mL while enhancing glutathione peroxidase and superoxide dismutase activities. Moreover, the LF restored intestinal health by modulating microbiota homeostasis, such as adjusting the Firmicutes/Bacteroidetes ratio and increasing the abundance of beneficial bacteria like Clostridia_UCG-014 and Alistipes. Metabolomic profiling indicated that the enhanced bioactivity of the LF was primarily attributed to the enrichment of phenolic acids, flavonoids and their derivatives postfermentation, including ethyl caffeate, gallic acid, kaempferol and isorhamnetin. In summary, these findings provided new insights into the potential application of LF as a functional food for mitigating skeletal muscle and intestinal aging. Full article

A chromium/amorphous carbon (Cr/(Cr/a-C)ml) nanostructured multilayer coating with a chromium sublayer was deposited on 42CrMo4 (1.7225,BDS EN ISO 683-2:2018), 100Cr6 (1.3505, BDS EN ISO 683-17:2024), and HS18-0-1 (1.3355, BDS EN ISO 4957:2018) alloy steels, selected for their use in contact-loaded components subjected to cyclic fatigue and intense wear. The coating was sputter deposited by MF pulsed magnetron sputtering under consistent process parameters. The resulting coating, approximately 1.8 μm thick, can significantly enhance the service life of these components. Adhesion was evaluated via the Daimler–Benz test, while coating homogeneity was confirmed through energy-dispersive spectroscopy, revealing a consistent chemical composition across sample surfaces. Raman spectroscopy indicated a high sp³/sp² ratio, confirming a dominant diamond-like carbon structure. Nanoindentation measurements verified the coating’s hardness, aligning with the observed structural properties. These results validate the process parameters for depositing a Cr/(Cr/a-C)ml coating on these alloy steels, achieving this study’s objectives. Full article

Background/Objectives: Systemic sclerosis-associated interstitial lung disease (SSc-ILD) and chronic obstructive pulmonary disease (COPD) are linked to multi-organ vulnerability involving the lungs, heart, and kidneys. This study aimed to compare the annual changes in pulmonary, cardiac, and renal parameters in patients with SSc-ILD and COPD across three consecutive years, using both individual biomarkers and integrated composite profiles. Methods: This observational longitudinal study included repeated assessments in 2023, 2024, and 2025. Functional, laboratory, and imaging parameters were collected: 6-min walk test (6MWT), SpO₂ (pre-/post-exercise), spirometry/CT lung volumes, gas exchange (pO₂/pCO₂/lactate), echocardiography [left ventricular ejection fraction (LVEF), estimated systolic pulmonary artery pressure (sPAP)], cardiac biomarkers (NT-proBNP, MR-proANP, hsTnT), renal markers [eGFR, creatinine, albuminuria, albumin-to-creatinine ratio (ACR)], heart rate variability (HRV), and renal CT densitometry. All markers were standardized (z-scores, higher values = worse). Subprofiles were generated and aggregated into three integrated profiles (cardiac, renal, pulmonary). Within-group dynamics were analyzed using the Wilcoxon signed-rank test (year-to-year deltas), between-group comparisons with the Mann–Whitney U test, effect sizes via Cliff’s delta, and multiple testing correction with the Benjamini–Hochberg false discovery rate (FDR). Results: Exercise tolerance declined in both groups: by 2025, 6MWT distance decreased by −10 m in SSc-ILD (p = 0.006; q = 0.010) and −20 m in COPD (p = 0.002; q = 0.004); post-exercise SpO₂ fell in both cohorts (both p < 0.001; q < 0.001). MR-proANP remained consistently higher in SSc-ILD across all years (p ≤ 0.005; q ≤ 0.028). sPAP increased in both groups, reaching higher values in COPD by 2025 (p = 0.007; q = 0.033). NT-proBNP and hsTnT increased over time, while eGFR declined, and ACR rose in both cohorts (both p < 0.001; q < 0.001). HRV (HF/total power) decreased by 2025. Composite profiles showed: in 2023, the cardiac profile was worse in SSc-ILD (δ ≈ 0.27; p = 0.011; q = 0.048), but differences diminished by 2025; the renal profile was initially worse in SSc-ILD but later shifted unfavorably in COPD; the pulmonary profile showed no consistent between-group differences. Conclusions: Over three years, patients with SSc-ILD and COPD exhibited concordant deterioration in pulmonary, cardiac, and renal function. Distinct leading markers emerged: desaturation during exercise and neurohormonal activation (MR-proANP) in SSc-ILD, versus reduced 6MWT and higher sPAP in COPD. These findings support the need for integrated monitoring of the cardio–pulmo–renal continuum. Limitations include the observational design, multiple comparisons, and absence of advanced repeated-measures modeling. Full article