Search Results (47)

Background and Objectives: Many “food–medicine homologous traditional Chinese medicines (TCMs)” have been shown to delay vascular aging. In this study, we will select “food–medicine homologous TCMs” with the most potential to delay human-origin vascular aging and predict their core targets and mechanisms. Methods: Human-origin vascular-aging-related genes were screened from the NCBI and Aging Atlas databases. Candidate “food–medicine homologous TCMs” were initially filtered by constructing a protein–protein interaction network, followed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses. Key targets were validated in the Gene Expression Omnibus database and further screened by least absolute shrinkage and a selection operator. Finally, molecular docking and molecular dynamics simulations identified core targets. Results: Ten core “food–medicine homologous TCMs” with potential to delay human-derived vascular aging were identified: Crocus Sativus L., Glycyrrhiza uralensis Fisch., Chrysanthemum morifolium Ramat., Astragalus membranaceus (Fisch.) Bunge, Sophora japonica L., Hippophae rhamnoides L., Portulaca oleracea L., Lonicera japonica Thunb., Citrus aurantium L. var. amara Engl., and Morus alba L. Further analysis indicated that β-Carotene within these core “food–medicine homologous TCMs” may represent a potential active component targeting matrix metalloproteinase-1, with its action potentially linked to the interleukin-17 signaling pathway. The present study highlights the new hypothesis that immunosenescence (Th17/IL-17) is involved in vascular aging, suggesting that the top ten core “food–medicine homologous TCMs” may delay vascular aging by regulating immune cell function. Conclusions: The top ten “food–medicine homologous TCMs” provide potential candidates for functional products that delay vascular aging and provide computationally predicted mechanistic insights and a scientific basis for novel therapies. Full article

In order to analyze the effect of environmental factors on the release of negative air ions (NAI) by green tree species, this study conducted an open top chamber (OTC) control test in Beijing. The tree species selected were Acer truncatum, Sophora japonica, Pinus bungeana, and Pinus tabuliformis. The experiment investigated the effects of environmental factors on NAI release under different relative humidity conditions. The results of the study showed that (1) the NAI release contribution (L), NAI release coefficient (n), NAI release rate (s), NAI instantaneous present amount (v), and total NAI release amount (Z) all showed positive responses to humidity. (2) Under constant temperature and light intensity, all five capability indicators increased with the humidity gradient (40–80%) and reached their maximum values at 80% humidity. (3) NAI release was positively correlated with humidity, and the correlation coefficients were: Pinus tabuliformis (R² = 0.33) > Sophora japonica (R² = 0.17) > Acer truncatum (R² = 0.15) = Pinus bungeana (R² = 0.15, p < 0.05). (4) Under constant temperature and light intensity, the NAI release contribution (L) and NAI release coefficient (n) responded most strongly to humidity in the 40–60% range, while the total NAI release amount (Z), NAI release rate (s), and NAI instantaneous present amount (v) responded more significantly in the 60–80% range. Acer truncatum showed the strongest response in terms of NAI release contribution (L) and NAI release coefficient (n), while Sophora japonica exhibited the most significant response in terms of NAI release rate (s), NAI instantaneous present amount (v), and total NAI release amount (Z). This study, conducted using an OTC, clarifies the independent role of humidity on NAI released by green tree species, providing a scientific basis for forest recreation and urban green space planning. Full article

[Objectives]: By performing genome assembly, annotation, comparative characterization, and phylogenetic analysis on the complete chloroplast genomes of E. tubulosa and E. japonica—two medicinal plants belonging to the genus Euchresta—this study aims to identify their differential genes, thereby providing fundamental research for screening candidate genes as DNA barcodes for species identification and facilitating the conservation of these endangered species. [Methods]: Illumina PE150 sequencing was performed. Chloroplast genomes (plastomes) were assembled and annotated with GetOrganelle/SPAdes. Comparative analyses assessed gene content, IR/LSC/SSC structure, repeat profiles, and codon-usage bias. Using related Fabaceae as references, we conducted mVISTA alignments and sliding-window nucleotide diversity (Pi) analyses to identify candidate DNA barcodes. Phylogenies from whole-plastome sequences were inferred with Maximum Likelihood, Bayesian Inference, and Maximum Parsimony. [Results]: The plastomes measured 153,960 bp (E. japonica) and 150,146 bp (E. tubulosa), with GC contents of 36.30% and 36.20%, respectively, each exhibiting a typical quadripartite structure. IR/SC boundaries were highly conserved without evident expansion or contraction. Repeat statistics were 20/30 palindromic repeats, 57/64 tandem repeats, and 156/159 simple sequence repeats (SSRs) in E. japonica/E. tubulosa, respectively. Leucine was the most frequently encoded amino acid, cysteine the least, and codon usage favored A/U at third positions. Five hypervariable loci—rps19, psbA, trnK, matK, and rps16 (Pi > 0.03)—were identified as candidate DNA barcodes. All trees consistently placed both species within Papilionoideae (Fabaceae) and recovered the closest relationship to Sophora macrocarpa. [Conclusions]: This study provides, for the first time, complete plastomes and candidate barcoding regions for two protected Euchresta species, supplying foundational resources for species identification, resource assessment, and conservation planning. Full article

Osteoarthritis (OA) is a degenerative joint disease characterized by chronic inflammation and progressive cartilage breakdown. This study investigated the synergistic protective effects of oleaster fruit extract (OE) and Sophora japonica L. fruit extract (SJE) against interleukin-1β (IL-1β) -induced inflammation in human chondrocytes. OE and SJE were tested individually and in combination at various ratios (3:1, 2:1, 1:1, 1:2, and 1:3). Among the tested mixtures, the 3:1 OE:SJE ratio exhibited the most pronounced synergistic effect. When used individually, the concentrations required to achieve 90% cell viability were 313.6 µg/mL for OE and 4135.8 µg/mL for SJE. In contrast, the same viability level was achieved with a combined treatment of 26.4 µg/mL OE and 8.8 µg/mL SJE, yielding a combination index of 0.25, indicative of strong synergy. The 3:1 OE:SJE combination also significantly suppressed inflammatory mediators, including nitric oxide, interleukin-6, and tumor necrosis factor-α. This treatment also led to the downregulation of matrix-degrading enzymes, such as matrix metalloproteinase (MMP)-9 and MMP-13, and it promoted the preservation of hyaluronan, a key extracellular matrix component. These findings suggest that the 3:1 OE:SJE combination exerts a synergistic protective effect by modulating both inflammatory and catabolic pathways and may represent a promising therapeutic strategy for OA prevention or treatment. Full article

Urban expressway interchanges, though primarily engineered for traffic efficiency, also serve as crucial ecological nodes within urban landscapes. This study evaluates the ecological functions of arborous vegetation across four typical interchange configurations—cloverleaf, single trumpet, double trumpet, and irregular—along the Nanjing Ring Expressway. Using the i-Tree Eco model, we quantified key ecosystem services, including carbon sequestration and storage, air pollutant removal, and stormwater mitigation. Field surveys documented 7985 trees from 45 species, with the 10 most abundant accounting for over two-thirds of total individuals. Results revealed that the trees sequester around 115 tons of carbon annually and store nearly 1850 tons in total, equivalent to an estimated economic benefit of ¥5.8 million. Trees also removed more than 1.5 tons of air pollutants and intercepted nearly 2400 cubic meters of stormwater each year. Species such as Sophora japonica, Phoebe zhennan, and Cinnamomum camphora emerged as key contributors to ecological performance. Among interchange types, double trumpet configurations yielded the highest overall service value, while single trumpet interchanges demonstrated superior efficiency per unit area. These findings highlight the underutilized ecological potential of transport-adjacent green spaces and underscore the importance of species selection and spatial design in maximizing multifunctional benefits. Full article

Negative air ions (NAIs) can purify the atmosphere and maintain human health. In this study, we selected six tree species, Pinus tabuliformis, Pinus bungeana, Acer truncatum, Sophora japonica, Koelreuteria paniculata, Quercus variabilis, Robinia pseudoacacia, and Populus tomentosa, and we established for the first time five “capacity indicators” to characterize and judge the capacity of plants to release negative ions: they comprised the release contribution rate L, release coefficient n, release rate s, instantaneous current number v, and total level of release Z. These indicators were used to assess the ability of the plants to release NAIs by themselves. The results showed the following. (1) The daily variations in L and n show “W” and “concave” shapes, respectively, and the contribution capacity at night is significantly higher than that during the day. The diurnal variations in s, v, and Z all showed a “bimodal” pattern. The NAI release rate and release level of each tree species during the day were significantly higher than those at night. (2) The trees released the most NAIs during the day at approximately 10:00, while Robinia pseudoacacia and Populus tomentosa peaked with a 2 h lag (12:00). The NAI release capacity of each tree species was the worst at 13:00. (3) During the growing season, the self-contribution effects L and n of the plants were the strongest in May. The release rates and release levels s, v, and Z were the lowest in August. The coniferous plants released NAIs at the fastest rate in September and broad-leaved plants in July, with the highest release levels. In this study, the plants released the most NAIs from 10:00 a.m. to 11:00 a.m., which is the best time to travel. Quercus variabilis was preferentially recommended in the pairing of species of tree with the quickest NAI release and the highest total number released, followed by Koelreuteria paniculata and Sophora japonica. Full article

Understanding species-specific mechanisms governing symbiotic fungal responses to plant traits and soil factors is critical for optimizing urban tree “plant-soil-fungus” systems under pollution stress. To address this gap, we combined δ¹³C/δ¹⁵N isotope analysis and ITS sequencing for three common street trees in Beijing: Sophora japonica, Ginkgo biloba, and Populus tomentosa. In S. japonica, symbiotic fungal abundance was positively associated with leaf δ¹⁵N, indicating root exudate-mediated “plant-microbe” interactions during atmospheric NO_x assimilation. G. biloba, with weak NO_x assimilation, exhibited a negative correlation between fungal abundance and soil available N/P, suggesting mycorrhizal nutrient compensation under low fertility. P. tomentosa showed decreased fungal abundance with increasing soil N/P ratios and specific leaf area, reflecting carbon allocation trade-offs that limit mycorrhizal investment. These results demonstrate that symbiotic fungi respond to atmospheric and edaphic drivers in a tree species-dependent manner. Urban greening strategies should prioritize S. japonica for its NO_x mitigation potential and optimize fertilization for G. biloba (nutrient-sensitive fungi) and P. tomentosa (nutrient balance sensitivity). Strategic mixed planting of P. tomentosa with S. japonica could synergistically enhance ecosystem services through complementary resource acquisition patterns. This study provides mechanism-based strategies for optimizing urban tree management under atmospheric pollution stress. Full article

Flower color serves as a vital ornamental feature of landscape plants; Sophora japonica L. mutant ‘AM’ exhibits the different colors from the common S. japonica. ‘AM’, presenting with a light purple-red wing and keel and yellowish-white flag petals, while common S. japonica is yellow and white. The metabolites contributing to this color specificity in red-flowered S. japonica ‘AM’ are not yet fully understood. In this study, the flag, wing, and keel petals were collected from ‘AM’ at various phases, including the flower bud phase, initial flowering phase, full bloom phase, and final flowering phase, for conducting the metabolic assays targeting anthocyanins. Subsequently, we identified 45 anthocyanin-related metabolites, including nine flavonoids and 36 anthocyanins. Ten major floral chromoside metabolites were found to affect the coloration differences among the petals, where the most abundant anthocyanin was cyanidin-3-O-glucoside (Cy3G), which was much higher in the keel petal (LGB) and wing petal (YB) than in the flag petal (QB), and similarly, during the four periods of different petal types, the Cy3G content was higher in the initial flowering stage (S2), the full bloom stage (S3), and the final flowering stage (S4) than the flower bud stage (S1), which was in accordance with the trend of the observed petal floral color phenotypic difference measurement correlation. This suggested that the Cy3G accumulation was the primary factor driving the distinct coloration of varying types of petals. These findings could contribute to the understanding of the biochemical mechanisms underlying S. japonica petal coloration and may support future efforts in flower color improvement. Full article

Roasting is the most common thermal processing method established for Sophora japonica (SJ) buds applied as traditional medicines, and it has also been reported to alter several of their therapeutic functions. However, there have been no studies investigating the influences of roasting on the effects of these materials against bacteria. Therefore our study was performed to examine the alterations that this process would induce in SJ buds’ antibacterial properties. Fresh buds were subjected to hot air drying or different roasting methods, as described in Materia Medica, including yellow-, dark yellow-, scorched-, and charred-roasting conditions. Antibacterial effects, total polyphenol and flavonoid contents, antioxidant activities, as well as rutin and quercetin concentrations in methanol extracts obtained from those materials, were then measured and compared. The results showed that dark yellow-roasted SJ buds exerted the strongest antibacterial and antioxidant activities and were also the richest in polyphenol contents. Analysis of rutin and quercetin revealed that, following the increment in heating temperatures up to 240 °C, the reduction in rutin content occurred in a parallel manner to the increment in quercetin content. However, overheating at 300 °C reduced both concentrations. Among the five tested samples, dark yellow-roasted SJ had the highest amounts of quercetin. Furthermore, the comparison of rutin and quercetin in antibacterial effects and antioxidant activities showed that the latter was significantly stronger in both of these functions, suggesting that the increment in quercetin content as a result of heat treatment was responsible, at least in part, for the potentiation of the two therapeutic effects. Full article

Sophora japonica is an important native tree species in northern China, with high ornamental, medicinal, and ecological value. In order to elucidate the genetic resources of ancient S. japonica, 16 simple sequence repeat (SSR) markers were used to evaluate its genetic diversity and population structure and build a core collection of 416 germplasms from the Shandong, Shanxi, and Hebei provinces. A total of 160 alleles were detected, the mean major allele frequency (MAF)was 0.39, and the mean effective number of alleles (Ne) was 4.08. Shannon’s information index (I), the observed heterozygosity (Ho), the expected heterozygosity (He), and the polymorphism information content (PIC) were 1.58, 0.64, 0.74, and 0.70, respectively, indicating relatively high genetic diversity in ancient S. japonica germplasms. Low genetic differentiation coefficient (Fst = 0.04) and frequent gene flow (Nm = 9.74) were found in the tested S. japonica populations, and an analysis of molecular variance (AMOVA) indicated that the genetic variation mainly came from within individuals (84%). A genetic structure and cluster analysis indicated that 416 ancient S. japonica germplasms could be divided into five subgroups, and there were obvious genetic exchanges among different subgroups. A core collection consisting of 104 (25% of the original collection) germplasms was constructed using the R language package Genetic Subsetter version 0.8 based on the stepwise regression method. The retention rates of the number of alleles (Na), Ne, I, He, and PIC were 87.50%, 106.24%, 103.02%, 102.50%, and 102.74%, respectively. The t-test analysis suggested that there were no significant differences between the core collection and the original collection. The principal coordinate analysis (PCoA) showed that the core collection was uniformly distributed within the initial collection and was able to fully represent the genetic diversity of the original collection. These results provide a scientific basis for the conservation and utilization of ancient S. japonica germplasms. Full article

The visual morphology of trees significantly impacts urban green micro-landscape aesthetics. Proximity to tall buildings affects tree form due to competition for space and light. The study investigates the impact of tall buildings on six visually morphological traits of eight common ornamental species in urban micro-landscapes in Beijing, with the distance and direction between trees and buildings as variables. It found that as trees grow closer to buildings, most angiosperms show increased crown asymmetry degree and crown loss, and reduced crown round degree and crown stretch degree (i.e., Sophora japonica L. and Acer truncatum Bunge saw a 52.26% and 47.62% increase in crown asymmetry degree, and a 20.35% and 21.59% decrease in crown round degree, respectively). However, the pattern of crown morphological changes in gymnosperms is poor (the closer the distance, the lower the height-to-diameter ratio of Pinus tabuliformis Carr., while the height-to-diameter ratio of Juniperus chinensis Roxb. significantly increases). In terms of orientation, gymnosperms on the west side of buildings have a greater crown asymmetry degree. It suggests that planting positions relative to buildings affect tree morphology. Recommendations include planting J. chinensis closer to buildings but keeping angiosperms like Fraxinus velutina Torr., S. japonica, and A. truncatum more than 3 m away to ensure healthy crown development. Full article

In view of global climate and environmental challenges, exploring sustainable urban vegetation management and development is crucial. This study aims to investigate the design strategies of urban road green space plants under the guidance of the dual theories of carbon sequestration and cooling eco-efficiency and aesthetics. In this study, Yangling, a representative small- and medium-sized city, was selected as the study area, and road green space plants were identified as the research objects. The assimilation method was employed to ascertain the carbon sequestration and oxygen release, as well as the cooling and humidification capacities of the plants. The aesthetic quality of the plants was evaluated using the Scenic Beauty Estimation and Landscape Character Assessment. Finally, we propose design strategies for landscapes with higher aesthetic and carbon sequestration and cooling benefits. The results demonstrate a clear nonlinear positive correlation. The carbon sequestration and cooling benefits of plants and the aesthetic quality, with correlation coefficients of 0.864 and 0.922, respectively. Across the same sample points, the rankings of standardized values for carbon sequestration, cooling benefits, and aesthetic quality vary minimally. This indicates that eco-efficient plants with harmonious colors and elegant forms can boost the aesthetic appeal and ecological function in road green spaces. Furthermore, the Sophora japonica Linn., Ligustrum lucidum Ait., Koelreuteria paniculata Laxm., Prunus serrulata Lindl., Prunus cerasifera Ehrhar f., Ligustrum sinense Lour., Photinia × fraseri Dress, Ligustrum × vicaryi Rehder, Sabina chinensis (L.) Ant. cv. Kaizuca, and Ophiopogon japonicus (L. f.) Ker Gawl. are proved to be ecologically dominant plants. They can be employed as the principal selected species for plant design. This study summarizes applicable design strategies for three types of green spaces: avenue greenbelts, traffic separation zones, and roadside greenbelts. The nonlinear regression model developed here provides a reference for scientifically assessing and optimizing urban planting designs. Full article

Large cities are typically characterized by a mosaic of green spaces that hold a remarkable variety of native and “exotic” plants. Urban beekeeping has gained increasing popularity. In order to characterize the “urban” in the honey, pollen diversity in 50 honey samples from 18 apiary locations in Vienna, Austria, was microscopically analyzed. The relative abundances of each plant taxon were determined by counting out 500 individual pollen grains per sample. In total, 202 taxa could be identified, with a median of 46 per sample. Taxa richness and diversity differed significantly across three years but did not so between urban and suburban apiaries. Despite trees comprising only roughly a quarter of all taxa, the amount of tree pollen was disproportionally high. The invasive Ailanthus altissima was predominant in 15 out of 50 samples. Other important non-native and/or ornamental trees included Sophora japonica, Gleditsia triacanthos, Castanea sativa, Koelreuteria paniculata, and Liriodendron tulipifera. Urban honey from Central Europe may typically comprise pollen taxa from Europe, East Asia, and North America alike. The results of this study show that intentionally planted, managed urban green spaces can support stable foraging resources for pollinators in cities. Full article

Environmental pollution is increasingly recognized as a significant contributor to skin and scalp damage. Oral supplementation with a specific blend of four standardized botanical extracts (Rosmarinus officinalis, Lippia citriodora, Olea europaea leaf, and Sophora japonica) has been previously demonstrated to enhance skin health in individuals exposed to high environmental stress. Thus, it might represent a convenient strategy to also improve their scalp health aspect, particularly in subjects with sensitive scalps. To support these effects, a 12-week double-blind, randomized, placebo-controlled trial was performed in 66 women working outdoors in urban areas with high particulate matter (PM) levels and taking 250 mg of the botanical blend daily. Product efficacy was measured as follows: skin antioxidant status (FRAP); skin and scalp moisturization (corneometer), transepidermal water loss (tewameter), and oiliness (sebumeter); skin radiance and colour (spectrophotometer), elasticity and firmness (cutometer) and wrinkle depth (image analysis); and scalp clinical evaluation. Despite constant exposure to increased levels of PM, the tested product positively influenced all monitored parameters compared to both baseline and the placebo-treated group, in as early as 4 weeks. At the end of the study, key improvements included increased skin FRAP (21.9%), moisturization (9.5%), radiance (24.9%) and reduced wrinkle depth (−16.5%), dark spot pigmentation (−26.2%), and skin oiliness (−19.3%). For the scalp, moisturization increased (14.1%), TEWL decreased (−13.8%), and sebum content reduced by 16.2%. Additionally, 71% of subjects with sensitive scalps experienced reduced redness. These findings highlight the extensive benefits of the ingredient, expanding its application beyond conventional skin treatments to also alleviate scalp issues. Full article

This study proposes a low-cost method for crown segmentation and forest inventory based on satellite remote sensing images and the deep learning model BlendMask. Taking Beijing Jingyue ecoforestry as the experimental area, we combined the field survey data and satellite images, and constructed the dataset independently, for model training. The experimental results show that the F1-score of Sophora japonica, Pinus tabulaeformis, and Koelreuteria paniculata reached 87.4%, 85.7%, and 86.3%, respectively. Meanwhile, we tested for the study area with a total area of 146 ha, and 27,403 tree species were identified in nine categories, with a total crown projection area of 318,725 m². We also fitted a biomass calculation model for oil pine (Pinus tabulaeformis) based on field measurements and assessed 205,199.69 kg of carbon for this species across the study area. Additionally, we compared the model to U-net, and the results showed that BlendMask has strong crown-segmentation capabilities. This study demonstrates that BlendMask can effectively perform crown segmentation and forest inventory in large-scale complex forest areas, showing its great potential for forest resource management. Full article