Search Results (379)

Improving the productivity and stability of winter wheat under increasingly variable climatic conditions remains a major challenge for sustainable agriculture. This study evaluated the effects of pre-sowing seed treatment with a microbial preparation (Nando BioExpert) and a biostimulant (Vitazyme), applied individually and in combination, on crop establishment, yield components, and grain yield of winter wheat under unstable moisture conditions in the Right-Bank Forest-Steppe of Ukraine. A three-year field experiment demonstrated that both treatments positively influenced plant growth, while their combined application produced a pronounced synergistic effect. Seed treatment enhanced plant establishment, increasing plant density at emergence from 242 plants m⁻² in the control to 372 plants m⁻² under the combined treatment. This improvement contributed to increased stand-level productive tiller density per unit area. Consequently, grain yield was consistently improved across years, with the combined treatment producing the highest average yield (6.04 t ha⁻¹), corresponding to a 37% increase relative to the control. The results indicate enhanced winter wheat resilience to environmental stress under biological seed treatment. Overall, integrating microbial inoculants with biostimulants represents an effective strategy for improving winter wheat productivity under moisture-limited conditions and supports the transition toward sustainable and resource-efficient crop production systems. Full article

This study characterized 58 Phaseolus spp. accessions conserved in the INIA–Amazonas Germplasm Bank (Peru) using an integrated agromorphological, physicochemical, and microstructural approach. Significant variability was observed among vegetative, reproductive, and seed-related traits, reflecting the broad phenotypic diversity of Andean germplasm. Cluster analysis identified groups with contrasting agronomic characteristics, particularly regarding plant height, number of pods per plant, and seed weight. Physicochemical analyses revealed significant differences in colorimetric parameters, phenolic content, and antioxidant activity among accessions. Darker-seeded accessions generally exhibited higher phenolic contents and greater antioxidant capacity. In addition, Fourier-transform infrared (FTIR) spectroscopy, rheological analysis, and scanning electron microscopy (SEM) revealed differences in molecular composition, starch functionality, and granule morphology among accessions. Overall, the evaluated germplasm exhibited substantial phenotypic and biochemical diversity, underscoring its potential value for breeding programs and food-related applications. These findings contribute to the conservation, sustainable utilization, and valorization of native bean genetic resources. Full article

Fraud detection models often achieve a strong ranking performance through black-box ensembles, but operational deployment also requires calibration, low explanation cost, and auditable scoring logic. This study develops an interpretable fraud-detection pipeline for IEEE-CIS by combining a 63-variable causal concept bank with teacher-guided additive Explainable Boosting Machine (EBM) students. The concept bank summarizes the temporal state, entity history, novelty/reuse, identity missingness, and aggregate deviation. Experiments use a chronological out-of-time split and a stricter pseudo-entity-disjoint holdout. In the main three-seed evaluation, the CatBoost predictive ceiling and XGBoost teacher achieved PR-AUC 0.489 ± 0.001 and 0.478 ± 0.003, respectively. Among interpretable models, concept-only EBM reached 0.189 ± 0.000, raw-only EBMs reached 0.372 ± 0.005 (top-k = 8) and 0.383 ± 0.002 (top-k = 12), and hybrid EBMs reached 0.407 ± 0.003 (top-k = 8) and 0.407 ± 0.004 (top-k = 12), consistently improving over matched raw-only additive baselines. The final top-k = 8 hybrid reduced input features from 154 to 71, achieved about 9.7× faster inference than XGBoost, remained close to XGBoost in ECE-15 calibration (0.01587 vs. 0.01611) while having a higher Brier score, and produced native local explanations far faster than XGBoost + SHAP. The results position CatBoost as the predictive ceiling and hybrid EBM as a benchmark-supported, deployment-relevant interpretable compromise for applied financial risk-screening workflows, rather than as a production-validated fraud-monitoring system. Full article

Weed competition, persistent seed banks, and management costs can limit crop productivity in organic farming. A two-year field experiment was conducted in southern Finland to evaluate the effects of pea (Lathyrus oleraceus Lam.), oats (Avena sativa L.), and camelina (Camelina sativa (L.) Crantz.), grown as pure stands and as three-crop mixtures at varying seeding densities, on weed diversity and suppression. The seeding densities (%) were 50:20:30 and 33:33:33 of the pure stand density of pea, oats, and camelina in 2022 and 50:50:50 and 33:33:33 of the pure stand density in 2023. Weed diversity was assessed at five sampling times, species were identified and analyzed for biomass, richness, Shannon-Wiener index (H), evenness, and dominance. Weed diversity and suppression varied with crop composition, growth stage, and seasonal conditions. In 2022, the 33% mix had the highest H (2.22) and evenness (0.77), enhancing weed suppression while controlling dominance. In 2023, pure oats had the highest H (1.65) and evenness (0.87), and pure peas had the lowest H (1.41) and evenness (0.67). Although pure oat stands provided the strongest weed suppression, crop mixtures enhanced species diversity and evenness, suggesting potential for more balanced weed management in organic systems, with short-term results indicating potential benefits for weed control. Full article

Background: Diabetes mellitus remains a major public health concern, particularly in sub-Saharan Africa where type 2 diabetes predominates. In West Africa, Uvaria chamae P. Beauv. is traditionally used for diabetes management. This study investigates previously reported metabolites from Uvaria chamae using an integrated in silico approach to explore their potential antidiabetic activity and underlying mechanisms. Methods: A comprehensive literature survey identified 106 phytochemicals from stems, roots, leaves, and seeds. Diabetes-related protein targets were retrieved from the RCSB Protein Data Bank, while ligand structures were obtained from PubChem and the COCONUT database. Molecular docking, MM-GBSA rescoring, induced-fit docking, QSAR, and ADMET analyses were performed to evaluate interaction profiles, predicted activity, and developability. Results: The integrated analysis supports a polypharmacological mixture-based profile with organ-associated trends. Stem- and root-derived flavonoids, particularly isouvaretin and diuvaretin, showed the most consistent profiles for PPARγ-related pathways, while uvarinol was associated with PTP1B. Leaf alkaloids were mainly linked to DPP-4 and digestive enzyme inhibition. These compounds displayed more favorable predicted pharmacokinetic and toxicity profiles compared to acetogenins, which, despite favorable binding energies, were not prioritized as drug-like candidates due to their high lipophilicity, low QED values, and predicted toxicity liabilities, but may contribute to extract-level activity. Conclusion: These findings provide a hypothesis-generating and hierarchical framework for the prioritization of Uvaria chamae metabolites and extracts, supporting further experimental validation through enzymatic, cellular, and gene expression studies. Full article

Leucaena leucocephala is a nitrogen-fixing legume widely used in agroforestry systems, although its invasive potential poses increasing risks to wetlands and riparian ecosystems. This systematic review synthesizes current knowledge on the ecological mechanisms, environmental stressors, and management strategies associated with the invasion of L. leucocephala in humid tropical environments. Following PRISMA guidelines, 60 studies retrieved from Scopus, Web of Science, and Consensus were qualitatively analyzed. The results indicate that invasion success is strongly associated with environmental disturbances and stress conditions, particularly drought stress, altered hydrological regimes, fire occurrence, and land-use change, which reduce ecosystem resistance and facilitate species establishment. Key invasion mechanisms include high seed production, persistent soil seed banks, rapid growth, allelopathic effects, and strong resprouting capacity, leading to suppression of native vegetation and structural simplification of plant communities. Integrated management strategies combining mechanical and chemical control with active revegetation consistently showed higher effectiveness than isolated approaches. The evidence further suggests that climate-related stressors may intensify invasion dynamics and increase ecosystem vulnerability under future climate scenarios. Despite recent advances, important knowledge gaps remain regarding long-term ecosystem functioning, hydrological feedback, and adaptive management in invaded wetlands. Full article

Large reservoir construction generates vast drawdown zones characterized by novel hydrological regimes that impose unprecedented selective pressures. While annual plants serve as pioneer colonists during secondary succession in these ecosystems, the mechanisms allowing their seeds to persist through prolonged anti-seasonal flooding remain poorly understood. We investigated how seed germination responses to extreme submergence are influenced by dormancy traits and phylogenetic history. We conducted a field experiment on 44 common annual plant species in the Three Gorges Reservoir drawdown zone. Seeds were subjected to maximum submergence depths of 0 m (control), 5 m, 10 m, 15 m, and 20 m, along the reservoir’s hydrological gradient. Post-submergence germination percentages were measured and analyzed using linear and Bayesian phylogenetic mixed-effects models, with seed dormancy status, seed type, season, and species’ phylogenetic relationships as explanatory variables. Submergence significantly reduced overall seed germination (p < 0.001), but more than 75% of species retained germination capacity even after 20 m of submergence. Germination percentage distributions shifted from near-normal to bimodal with increasing depth. Although the regression of squared PIC values against phylogenetic branch lengths showed a significant relationship, phylogenetic signal for germination percentages was weak and non-significant across all depths (Pagel’s λ < 0.101, Blomberg’s K < 0.228, p > 0.05). Bayesian models revealed that dormancy season significantly interacted with submergence depth (Estimate = −1.41, 95% CrI [−2.16, −0.67]). Seeds dormant during autumn-winter maintained stable germination percentages across depths, while germination of spring-summer dormant seeds declined significantly with increasing depth. Our findings demonstrate that annual plant seeds possess widespread, species-specific tolerance to extreme submergence. This tolerance is primarily driven by environmental filtering rather than phylogenetic history. The seasonality of dormancy is a crucial adaptive mechanism, enabling seeds, particularly those dormant in autumn-winter, to withstand the harsh conditions of the Three Gorges Reservoir drawdown zone. This study provides a functional trait-based framework for selecting suitable species for the ecological restoration of reservoir drawdown zones globally. Full article

Reservoir drawdown zones are repeatedly affected by water-level fluctuations and anthropogenic regulation, making vegetation recovery an important issue for ecological restoration and sustainable reservoir management. This study focused on Cynodon dactylon, a dominant herbaceous species in the drawdown zones of five reservoirs in the Jinsha River Basin, southwestern China. Drawing on the existing concept of stress memory, which emphasizes the retained effects of previous environmental stress exposure on subsequent plant responses, we established an integrated assessment framework based on species dominance, functional traits, landscape pattern indices, and the soil seed bank. This framework was used to evaluate variation in the stress memory of C. dactylon across different successional stages and inundation gradients. The results showed that the overall stress memory of C. dactylon increased with successional progression in both the upper and lower zones, indicating continuous adaptive accumulation under long-term hydrological disturbance. The memory reflected by individual component indicators also generally increased, although their accumulation patterns varied among indicators. These findings suggest that dominance, functional traits, landscape pattern, and the soil seed bank can jointly characterize the adaptive responses of C. dactylon during vegetation recovery. Overall, the stress memory framework provides a systematic approach for identifying stage-specific vegetation changes, evaluating restoration potential, and informing ecological restoration and sustainable management in reservoir drawdown zones. Full article

Temperature and humidity are the key environmental factors affecting the storage life of seeds. To explore the feasibility and factors influencing ultra-dry storage of Pinus kesiya var. langbianensis seeds, the seeds were dehydrated to six different moisture contents (0.92–6.12%) and stored for one year. The effects of moisture content, packaging method, storage temperature, and pre-humidification method on the viability of ultra-dry seeds were systematically investigated using an orthogonal experimental design. The germination energy, relative electrical conductivity (REC), malondialdehyde (MDA), proline (Pro), total soluble sugar content, and fatty acid composition were determined. The results showed that moisture content and pre-humidification had significant effects on seed germination energy and vigor (p < 0.01). The germination energy of ultra-dried seeds was significantly negatively correlated with REC and MDA contents (p < 0.01) and significantly positively correlated with Pro content (p < 0.01). Based on the comprehensive indices, the optimal combination for seed germination energy was: 4.24% moisture content, self-sealing bag packaging, room temperature (25 °C) storage, and 20% polyethylene glycol (PEG) pre-humidification. Under the optimal moisture content (4.24%), the total sugar content of seeds was the lowest, while the fatty acid unsaturation index and oleic acid content were higher than those in the other treatments. Therefore, appropriate ultra-dry treatment can effectively maintain the seed vigor of P. kesiya var. langbianensis, and its protective effect is closely related to reducing membrane lipid peroxidation, accumulating proline, and regulating fatty acid unsaturation. This has important implications for forest seed conservation and germplasm management, particularly for long-term ex situ preservation of tree seeds in gene banks, supporting reforestation and biodiversity restoration efforts. Full article

To enhance the utilization efficiency of limited ecological water, this study conducted field ecological irrigation experiments in a typical desert riparian forest in the lower reaches of the Tarim River. Based on the experimental data, a soil water transport model under the overflow irrigation mode was constructed using the HYDRUS-2D (version 2.04) model. Based on the model, numerical simulation scenarios of different irrigation schemes were designed to provide key evidence for the scientific utilization of water resources in the ecological restoration of desert riparian forests. Simulation results indicate that (1) more irrigation water does not necessarily yield better results. When the total irrigation volume is the same, conducting overflow irrigation in two separate applications significantly outperforms a single concentrated irrigation in terms of soil moisture replenishment and maintenance, with an optimal interval of 20 h between applications. (2) For single overflow irrigation, the optimal water depth is 5 cm. (3) For two-stage irrigation, the available water resources and core objectives must be considered. When water is plentiful, and it is necessary to replenish moisture in the lower soil layers, the 5 cm + 5 cm scheme is optimal; if irrigation water is limited, the 3 cm + 3 cm scheme is more efficient. These schemes can effectively activate the seed bank in the surface soil while supplying water to the root systems of desert riparian vegetation, thereby promoting the restoration and growth of desert vegetation and achieving the goal of ecological sustainability. Full article

The durian seed borer, Mudaria stahlgretschae, is a major economic pest that has significantly impacted durian cultivation in Southeast Asia; however, comprehensive biological and ecological data for this species remain limited. This study employs an integrative taxonomic approach, combining morphological examination with molecular validation of the mitochondrial cytochrome c oxidase subunit I (COI) gene. Phylogenetic analysis (Neighbor-Joining) confirmed that all collected specimens (n = 11) formed a distinct monophyletic clade within the genus Mudaria, showing a genetic identity of 95.75–96.85% with existing GenBank accessions, thereby confirming their identity as M. stahlgretschae. Systematic monitoring using light traps in Uttaradit Province revealed a clear seasonal phenology, with adult flight activity restricted to a five-month period from April to July 2025. Population density peaked in May (55.56%), synchronized with the mid-stages of durian fruit development. Furthermore, chemical profiling of female gland volatiles via GC-MS identified 40 compounds; among these, four putative sex pheromone candidates—1-Hexacosene, (Z)-7-Hexadecenal, 11-Octadecenal, and 2-Hexadecanol—were identified as key constituents based on their consistent detection across all replicates (n = 3), high relative abundance, and absence in male extracts or blank controls. These findings establish a critical foundation for developing synthetic pheromone lures and synchronized monitoring programs, offering a robust framework for the sustainable management of M. stahlgretschae in durian agroecosystems. Full article

The Republic of Korea has implemented routine vaccination against foot-and-mouth disease virus (FMDV) in livestock using a bivalent vaccine comprising serotypes O and A following the massive FMD outbreak in 2010, while antigens for the remaining serotypes are maintained in overseas antigen banks. The recent geographic expansion of FMDV Southern African Territories 1 (SAT1) beyond Africa underscores the need for enhanced preparedness in previously unaffected regions. In this study, we evaluated the SAT1 BOT-R strain as a candidate vaccine seed for potential domestic vaccine production by optimizing antigen production conditions, assessing scalability, determining virus inactivation parameters, and examining immunogenicity in pigs. Optimal antigen yield was achieved at 20 h−24 h post infection with a multiplicity of infection of 0.005−0.01, with production remaining stable under mildly alkaline conditions. Antigen productivity was consistently maintained during scale-up from shake flasks to a bioreactor, yielding up to 9.5 μg/mL. Complete virus inactivation was achieved using binary ethylenimine at 2 mM for 24 h at 26 °C. Vaccines formulated from both flask- and bioreactor-derived antigens elicited comparable neutralizing antibody responses in pigs, reaching a median titer of 1:500 following booster immunization. Collectively, these findings demonstrate that the SAT1 BOT-R strain is a viable and scalable candidate for SAT1 antigen banking and future domestic vaccine production, providing a practical framework for strengthening national preparedness against potential incursions of FMDV SAT1. Full article

Phytolacca acinosa Roxb., a perennial herb native to East Asia, is increasingly naturalizing in Europe, yet its reproductive ecology in the secondary range remains poorly understood. This study evaluated seed productivity across central and edge populations in the secondary range, fruit and seed morphometrics, and germination responses to cold storage, acid scarification (simulating bird endozoochory), and light exposure. Fruit production per raceme was influenced by an interaction between insolation and range position: reduced insolation increased fruit set in central populations but decreased it at the range edge. Raceme number per shoot was lower in spontaneous plants compared to cultivated ones. Fresh seeds exhibited strong dormancy with no germination without scarification. Acid scarification significantly enhanced germination, particularly with light exposure, reaching up to 55%. Cold storage did not increase germination percentage but accelerated germination of scarified seeds under light, reducing median germination time from 24 to 21 days. Compared to the congeneric P. americana, P. acinosa shows more stringent dormancy requirements. We conclude that P. acinosa retains deep seed dormancy in its secondary range and relies on bird-mediated endozoochory for both dispersal and dormancy release. At the northern range edge, reduced plant vigor and lower raceme numbers are partially offset by increased flower production per raceme, though fruit set remains constrained. The species does not exhibit the simplified germination requirements often associated with successful invaders; instead, its invasion success appears driven by a bet-hedging strategy combining persistent seed banks with specific dormancy-breaking cues. Full article

Alzheimer’s disease (AD) is characterized by progressive cognitive decline and the accumulation of amyloid-β (Aβ) plaques and tau neurofibrillary tangles. Beyond genetic and proteostatic mechanisms, infection- and dysbiosis-based models of AD have gained renewed attention, including the antimicrobial protection hypothesis, in which Aβ may participate in innate immune defense. Here, we reanalyzed ribosomal depleted (Ribo-Zero) RNA-seq data from dorsolateral prefrontal cortex (DLPFC) samples from the Mount Sinai Brain Bank cohort (GSE53697) to screen for non-human transcripts. Reads underwent quality control and adapter trimming, taxonomic classification with Kraken2, abundance re-estimation with Bracken, and differential abundance testing with edgeR. Across 17 samples (9 advanced AD and 8 controls), we detected low-biomass microbial signals, with Acinetobacter radioresistens showing enrichment in the AD group (FDR = 0.018). Several additional taxa showed suggestive group differences but did not remain significant after multiple testing correction, including Lactobacillus iners (FDR = 0.051). We also performed an exploratory in silico analysis of an A. radioresistens biofilm-associated protein homolog, identifying predicted amyloidogenic motifs and surface-exposed regions that may be relevant to cross-seeding hypotheses, although no mechanistic inference can be drawn without experimental validation. Given the technical challenges of inferring microbial signals from post-mortem brain RNA-seq data, including contamination risk, low microbial biomass, and overwhelming host background, these findings should be interpreted as hypothesis-generating and warrant orthogonal validation in larger, microbiome-aware cohorts. Full article

Extreme droughts are projected to become more frequent and severe under climate change, posing significant risks to wetland ecosystems and submerged macrophyte communities. We combined field surveys in West Dongting Lake, China, combined with controlled greenhouse experiments to examine how drought intensity (expressed as contrasting soil moisture conditions during drought) and drought timing affect submerged macrophyte species richness, biomass, as well as resilience, mediated through species response in their reproductive strategies. Field observations revealed a sharp decline in clonal species (Hydrilla verticillata, Ceratophyllum demersum, Vallisneria spinulosa) following an extreme drought, while the sexual species Najas marina emerged as dominant. Greenhouse experiments confirmed these patterns and elucidated underlying mechanisms: extreme drought suppressed biomass, leaf area, and seedling re-germination in clonal species, whereas N. marina maintained regeneration via a persistent soil seed bank. Moderate drought enhanced leaf area, consistent with the intermediate disturbance hypothesis, while early drawdowns were most detrimental to growth. Species-specific responses highlight the role of reproductive strategy in drought resilience. These findings underscore the need for climate-adaptive water-level management, including limiting early drawdowns, mitigating extreme drought, and conserving seed banks to sustain biodiversity and ecosystem function under increasing hydroclimatic variability. Full article