Search Results (932)

Dermatophytosis remains one of the most prevalent superficial fungal infections worldwide and is increasingly encountered as a persistent or difficult-to-treat syndrome. A major clinical problem is that apparent treatment failure is often attributed to antifungal resistance, although many cases are instead driven by diagnostic uncertainty, corticosteroid-modified disease, reinfection, inadequate exposure, poor adherence, and limited drug delivery within keratinized tissues. This narrative review was developed to clarify the distinction between true antifungal resistance and clinical recalcitrance, with particular attention to terbinafine-resistant Trichophyton species, Trichophyton indotineae, tinea incognito, onychomycosis, dermatophytoma, and high-barrier skin and nail infections. We synthesized peer-reviewed literature and guideline-level evidence addressing epidemiology, molecular mechanisms of resistance, clinical phenotypes of recalcitrance, diagnostic escalation, therapeutic decision-making, and antifungal delivery in keratinized tissues. The review contributes a dermatology-centered conceptual framework in which persistent dermatophytosis is interpreted through both microbiological resistance and modifiable recalcitrance drivers. This approach emphasizes confirmation of fungal disease when indicated, phenotypic and anatomic classification, avoidance of inappropriate corticosteroid combinations, optimization of dose, duration, vehicle, and adherence, measures to improve drug access and reduce protected fungal burden in high-barrier disease, and prevention of reinfection from reservoirs. The proposed framework may support more rational antifungal use and reduce unnecessary escalation; however, it is based on narrative synthesis rather than a systematic review or prospective validation. Additional studies are needed to determine how such structured clinical approaches affect clinical outcomes, relapse rates, antifungal exposure, and resistance emergence in real-world dermatology practice. Full article

This study develops exact analytical solutions for transient elliptical groundwater flow toward an extended well in an anisotropic fractured confined aquifer and then discusses how the resulting hydraulic response can support groundwater-head interpretation in shale-gas development areas. The environmental connection is made at the aquifer-protection scale: the model is not a shale-gas reservoir production model, and it does not solve contaminant transport directly. Instead, it provides a hydraulic interpretation framework for estimating anisotropy, equivalent fracture length, wellbore-storage effects, and the preferential direction of head propagation around possible leakage points, old wells, fractures, or monitoring wells. Based on Mathieu-function theory and the separation-of-variables method, constant-rate and constant-head solutions are derived in Laplace space and inverted to the time domain with the Stehfest algorithm. The analytical results are validated against COMSOL5.2 finite-element simulations, and the effects of anisotropy coefficient and wellbore storage are analyzed through drawdown and flow-rate type curves. A synthetic but field-style water-head example is included to demonstrate how monitoring records can be converted to drawdown, fitted to the elliptical-flow solution, and used to delineate a preliminary hydraulic response zone. The results show that anisotropy mainly controls early-to-middle time response, whereas wellbore storage may obscure early head changes and delay the recognition of fracture connectivity. Therefore, the solution is best regarded as a rapid hydraulic-screening and monitoring-design tool that can precede, but not replace, site-specific contaminant-transport modeling in shale-gas groundwater-protection studies. The relevant technical issues are possible head disturbances and preferential groundwater pathways associated with surface spills, flowback-water handling, old wells, faults, and fracture-connected water-bearing zones. Because verified local field-monitoring records were not available for us, the application example is explicitly described as a synthetic field-style demonstration; it is used to show the workflow and its limitations, not to claim site-specific prediction of contaminant concentration. Full article

Deep unconventional oil and gas reservoirs are critical to hydrocarbon exploration and development in China. However, their complex geological and petrophysical features, including high temperature, high pressure, high salinity, multiple pressure systems, and intricate pore–fracture structures, make them highly susceptible to formation damage during drilling, completion, stimulation, and production. Effective reservoir protection is therefore essential for minimizing damage and improving development efficiency. This paper systematically reviews recent advances in reservoir protection for deep unconventional reservoirs, with a focus on evaluation methods and protective materials. Laboratory evaluation methods, including permeability recovery, nuclear magnetic resonance, pressure decay, and spontaneous imbibition, together with field-based approaches such as well testing and production decline analysis, are summarized and assessed for their applicability to complex damage characterization. Major damage mechanisms, including liquid-phase trapping, solid invasion, sensitivity damage, stress sensitivity, and wettability alteration, are analyzed with emphasis on working fluid–reservoir interactions under multi-field coupling conditions. Recent progress in protective materials is also reviewed, covering polymer-based materials such as gel sealing agents, delayed-swelling hydrogels, water-/oil-soluble temporary plugging agents, and film-forming polymers, as well as ultrafine CaCO₃ and fiber-based materials. In addition, related protection technologies, including temporary plugging, film-forming fluid-loss control, underbalanced drilling, and low-damage completion fluids, are discussed. Existing models developed for conventional sandstone reservoirs are insufficient for deep unconventional systems. Future research should prioritize integrated evaluation and protection methods tailored to deep tight, shale, and fractured–vuggy carbonate reservoirs. This review provides a basis for understanding complex damage mechanisms, developing functional protective materials, and advancing integrated reservoir protection technologies for the efficient development of deep unconventional resources. Full article

Introduction: Freshwater fish communities in Mediterranean basins have undergone profound changes over recent decades due to biological invasions, habitat alteration, and hydrological regulation. The Segura River basin (SE Spain), particularly in the Region of Murcia, represents a paradigmatic case of these transformations, with increasing pressures on native ichthyofauna and growing relevance for recreational fisheries. Objective: This study aims to provide an updated inventory of fish communities in the rivers and reservoirs of the Region of Murcia, assessing current composition, relative abundance, and conservation status. Methodology: Fish assemblages were surveyed during sampling campaigns conducted in autumn 2023, 2024 and 2025. Sampling sites included representative reservoirs and river reaches within the middle sector of the Segura River basin, focusing on areas of interest for recreational fishing. Passive fishing gears were used in reservoirs, while electrofishing was conducted in riverine habitats. Presence and relative abundance data were recorded for all detected taxa. Results: A total of 15 taxa were identified (8 in reservoirs and 15 in river reaches). Reservoir communities were almost entirely dominated by non-native invasive species, including Cyprinus carpio, Alburnus alburnus, Sander lucioperca, Micropterus salmoides, Lepomis gibbosus, and Gambusia holbrooki, with Luciobarbus sclateri as the only native species. Riverine sections of the main channel also showed a strong dominance of exotic taxa, with additional species such as Esox lucius, Oncorhynchus mykiss, Gobio lozanoi, and Pseudochondrostoma polylepis. Native species detected included L. sclateri (dominant), Anguilla anguilla, and Salariopsis fluviatilis, the latter showing a recent expansion likely linked to human-mediated introduction. Isolated tributaries hosted the most valuable assemblages, including populations of L. sclateri, Squalius tartessicus, and the endangered Valencia hispanica (recently unauthorized translocations into the Segura river basin). Conclusions: Current fish communities in the Region of Murcia reflect a marked ecological degradation compared to historical conditions, driven by the proliferation of non-native species and habitat alteration. Changes over the last 30 years have been particularly pronounced in reservoirs and regulated sections of the main river channel, where local extinction of the native S. tartessicus has also been detected. Only the headwaters of certain tributaries retain fish assemblages of notable conservation interest, highlighting their priority for management and protection. Full article

Introduction: Understanding the movement ecology of invasive species such as the European catfish Silurus glanis, with documented strong impacts on freshwater fish communities, is essential to improve the effectiveness of management and containment actions, as detailed knowledge of species spatio-temporal habitat use strongly influences the success of control strategies. Objective: This study aimed to investigate the spatial and temporal behaviour of the S. glanis in a river–reservoir system in Portugal, including the Ponsul River and part of the Tagus River within the Cedillo reservoir, and to provide ecologically relevant insights to support targeted management strategies. Methodology: Acoustic telemetry was used to monitor 27 tagged individuals equipped with depth sensors. Fish movements were tracked using an array of 17 acoustic receivers over one and a half years. Results: Three behavioural profiles were identified: a resident group in the lower Ponsul (n = 4), a group moving between the Tagus River and the lower Ponsul (n = 6), and a larger group primarily migrating within the Ponsul River (n = 12). The remaining five individuals were considered dead, due to illegal fishing in this protected area. Migratory individuals showed a clear seasonal pattern, moving downstream to deeper waters during early winter and returning upstream to shallower areas as temperatures increased in early spring, likely in response to thermal gradients. Distance-based analyses confirmed this trend, with minimum inter-individual distances occurring in winter and early summer. Vertical behaviour supported this pattern, with individuals occupying shallow waters (≤7 m) for most of the year and reaching depths of up to 30 m in winter. Conclusions: The observed preference for shallow habitats during warmer periods and downstream migration in winter indicates that eradication efforts should be spatially and temporally targeted. Control actions should prioritize upstream sections during warm seasons and downstream areas of the Ponsul during winter migration, focusing efforts on traditional methods such as large-mesh multimesh gillnets or new longline techniques. Overall, this study highlights the value of telemetry in supporting targeted, evidence-based management of invasive species. Full article

The Jabal Ali Marine Sanctuary, Dubai, is one of the most important marine protected areas (MPAs) in the UAE. The Arabian Gulf is characterised by extreme environmental conditions, including high temperatures and hypersaline waters. These conditions, combined with increasing anthropogenic pressures from coastal development projects such as desalination plants, energy plants and the Palm Jebel Ali development, may influence the pelagic ecosystems of MPAs. This study examined seasonal variability in phytoplankton communities and environmental conditions between summer (June 2017) and winter (December 2017), with particular emphasis on the interactions between temperature-driven stratification, hypersaline conditions, and phytoplankton community structure, abundance, and diversity. The AZTI (AZTI Tecnalia Marine Research Centre) Marine Biotic Index indicated predominantly “Good” to “High” ecological status of the pelagic ecosystem, indicating favourable environmental conditions. Potentially harmful algal bloom taxa, including Pseudo-nitzschia and Dinophysis, were detected at low abundances. Summer surveys recorded higher total species richness (44 vs. 34 species) and greater phytoplankton abundance (mean 68.6 vs. 49.8 cells/L) compared to those in winter. Diatoms dominated the assemblages in both seasons, accounting for 62–69% of the recorded species, while distinct spatial zonation patterns reflected habitat heterogeneity. The observed seasonal and spatial variability highlight the importance of incorporating temporal and spatial dimensions into management strategies. As the first pelagic phytoplankton assessment conducted in an MPA, this study provides important baseline data for understanding phytoplankton ecology in one of the world’s most environmentally extreme marine ecosystems. The findings contribute to evidence-based management under increasing climate change and anthropogenic pressures. However, because sampling was limited to the two principal climatic seasons, the study characterises inter-seasonal variability rather than a complete annual succession cycle. Additional surveys during spring and autumn are recommended to fully resolve seasonal succession dynamics. Overall, the findings support the continued protection of the sanctuary as an important biodiversity reservoir and a potential reference site for assessing marine ecosystem responses to environmental conditions. These findings are directly relevant to the environmental sustainability agenda of the Dubai 2040 Urban Master Plan, which prioritises the protection and expansion of the emirate’s nature reserves and the safeguarding of marine and coastal biodiversity. By establishing the first pelagic phytoplankton baseline for the sanctuary, this study provides an evidence base for monitoring and managing marine protected areas in line with this long-term framework. Full article

Temperate broadleaved forests support diverse arthropod communities, but canopy-dwelling insects in European lime (Tilia cordata Mill.) stands are still poorly known. We surveyed light-attracted canopy insects in six T. cordata Genetic Conservation Units and related protected stands across Lithuania. One modified, solar-powered UV light trap was installed in the canopy (10–15 m) at each site and operated twice per month from June to August in 2023 and 2024. We used diversity metrics, similarity indices, multiple regression, and non-metric multidimensional scaling (NMDS) together with PERMANOVA to examine the structure of insect communities and assess the influence of environmental factors. In total, 6031 individuals representing 295 insect species were recorded, with higher abundance, species richness and Shannon diversity in 2024 than in 2023. Across both years and all sites, Shannon H diversity index ranged from 3.21 to 3.92. Sørensen indices indicated moderate species similarity among sites and distinct species composition at the Ukmergė genetic reserve. The 20 most abundant taxa comprised over 60% of all individuals, and dominance structure changed markedly between years: Serica brunnea dominated in 2023 but was nearly absent in 2024. Regression revealed a significant positive effect of air temperature on insect abundance (about a 31% increase per 1 °C), while precipitation had no significant effect on insect abundance. NMDS and PERMANOVA showed strong spatial structuring, with sites explaining most of the variation, and weaker but significant temporal and site-by-year effects. Overall, insect diversity metrics showed non-significant correlations with T. cordata genetic diversity parameters. Results demonstrate that mature T. cordata forest stands are important reservoirs of canopy insect diversity and highlight pronounced spatial heterogeneity, interannual dynamics, and temperature sensitivity of canopy assemblages in Lithuanian forests. Full article

Introduction: The invasive European catfish (Silurus glanis) is actively spreading across Iberian freshwaters, with no effective management measures in place to control its growing abundance or prevent its establishment in new localities. It poses a severe threat to endemic and already endangered species, and is simultaneously a preferred target by few anglers who continuously promote its spread. The LIFE-PREDATOR project aims to stop the spread of European catfish in lentic systems in Portugal and Italy, particularly in protected areas. Objectives: This talk will present the mid-term results of the LIFE-PREDATOR in Portugal, and discuss the difficulties and future challenges to reduce the size of local populations of European catfish. Methodology: The LIFE-PREDATOR team developed several tasks in Portugal: (1) established the reference situation of fish communities in six reservoirs in the Tagus Basin, using scientific fishing, fish telemetry and eDNA-based tools; (2) determined the optimal protocols for sampling catfish; (3) implemented an early detection programme based on warning teams, data-mining and eDNA tools; (4) developed population control actions in four reservoirs; and (5) organised dissemination events for the general public, anglers, and students from kindergarten to university levels. Results: Overall, there is a grim view about recipient communities in the studied lentic systems, which tend to be dominated by invasive fish species, including common carp (Cyprinus carpio), gibel carp (Carassius gibelio), European catfish, pikeperch (Sander lucioperca), European perch (Perca fluviatilis) and largemouth bass (Micropterus nigricans). At least three new localities harbouring catfish were identified from online data-mining and warning teams. A total of 8 tons of catfish were removed by mid-June of 2025, mostly from the Natural Park of International Tagus. Outreach activities were conducted in nearly 60 schools, reaching more than 5000 students. Moreover, 67 general public events have reached more than 4500 people since the project started (September 2023). Conclusions: Despite its positive outcomes, the LIFE-PREDATOR team has encountered challenges in engaging key stakeholders such as anglers, involving local municipalities, and implementing catfish removal actions in remote areas. Difficulties and challenges in catfish management must therefore be debated in order to assure the after-LIFE implementation across Portuguese protected areas. Full article

Introduction: Sediment flushing is widely used to recover storage capacity and maintain outlet functionality in Mediterranean reservoirs, but it can also generate short downstream pulses of suspended sediment, oxygen depletion, and ammonia that may threaten fish and fish habitats. Despite this, operation-specific environmental criteria explicitly oriented to reducing acute fish risk during flushing remain poorly defined. Objective: This study aimed to develop and validate a practical operational protocol for sediment flushing in southern Spain, with emphasis on reducing acute downstream risk to fish through field-applicable thresholds, decision rules, and stop criteria. We also evaluated whether water density could serve as a rapid surrogate for total suspended matter (TSM) during operations. Methodology: The protocol was applied to 14 flushing events conducted at seven reservoirs and weirs in Andalusia, southern Spain. Monitoring included upstream and downstream stations, pre-operation baseline surveys, 15-minute measurements during flushing, and post-operation recovery checks. Operational control was based on pre-alert and alert thresholds for dissolved oxygen, ammonium/ammonia, conductivity, suspended matter, and a density-based surrogate for TSM. Protocol validation considered operational safety during flushing, the relationship between field density and laboratory-measured TSM, and before–after multivariate changes in downstream environmental conditions. Results: Threshold exceedances occurred in 5 of the 14 events, comprising 4 pre-alerts and 1 alert. Pre-alerts were mainly driven by ammonium/ammonia or dissolved oxygen, and exceedance durations were generally short (30–120 min). The only alert-level event combined severe oxygen depletion with high sediment concentrations and triggered suspension of the operation, showing the usefulness of the stop rule. Density was significantly related to laboratory TSM in all reservoirs retained for calibration (R² = 0.365–0.934), supporting its use as a rapid field proxy when calibrated at the reservoir scale. Before–after multivariate analysis detected no consistent overall downstream shift, although event-level responses were heterogeneous. Conclusions: The protocol proved operationally feasible as a science-based framework for managing sediment flushing while reducing acute risk to downstream fish in Mediterranean reservoirs. Its combination of fish-relevant thresholds, real-time monitoring, site-specific density calibration, and explicit stop rules can support safer operations, improve transparency, and strengthen environmental permitting. Full article

Streptococcus pneumoniae remains a major global health threat and is listed by the World Health Organization as a pathogen in urgent need of new antimicrobial strategies. While primarily considered an extracellular pathogen, S. pneumoniae can persist within splenic macrophages in severe disease, creating a protected intracellular niche that may contribute to fulminant sepsis. We recently demonstrated the concept of an mRNA-based therapeutic approach in which host cells produce the pneumococcal bacteriophage endolysin Cpl-1. Here, we investigated whether expression of Cpl-1 in macrophages can target S. pneumoniae residing within host cells. Using the human THP-1 macrophage line, we demonstrated successful translation and intracellular accumulation of bioactive Cpl-1 following IVT-mRNA transfection. Lysates from Cpl-1 mRNA-transfected cells exhibited bacteriolytic activity, and Western blotting as well as immunofluorescent staining confirmed cytosolic endolysin production. Phagocytosis assays using an encapsulated and unencapsulated pneumococcal strain showed a reduction in intracellular bacterial burden in Cpl-1 mRNA-transfected macrophages compared with control and inactive-mutant Cpl-1 mRNA groups, and a flow cytometry-based assay further corroborated a decreased intracellular bacterial signal. Together, these findings suggest that mRNA-encoded Cpl-1 enhances intracellular killing of S. pneumoniae and supports the feasibility of mRNA-based endolysin therapies to target intracellular pneumococcal reservoirs. Full article

Deep tight sandstone reservoirs are characterized by strong microscopic pore structure heterogeneity and commonly exhibit a high-porosity, low-permeability profile, posing significant challenges for effective reservoir evaluation and “sweet spot” prediction. The microscopic pore structure of 209 tight sandstone samples from the deeply buried Huagang Formation in the Xihu Depression, East China Sea Basin, was systematically characterized by integrating multiple analytical techniques, including casting thin sections, scanning electron microscopy (SEM), X-ray diffraction (XRD), nuclear magnetic resonance (NMR), and high-pressure mercury injection (HPMI). The results indicate that the reservoir space is dominated by mesopores (55.48%) and transition pores (32.39%), with macropores (2.09%) and micropores (10.04%) being relatively underdeveloped. A significant vertical heterogeneity in reservoir quality is observed. The H4 member exhibits superior properties, characterized by a higher average movable fluid saturation (averaging 46%) and better pore connectivity. In contrast, the H5 member is more compact, with a notably higher proportion of bound fluid (averaging 47%). The differences in reservoir quality are controlled by a sedimentary–diagenetic coupling mechanism. High-energy, coarse-grained facies underwent a constructive pathway involving chlorite coating protection and dissolution enhancement, forming high-quality pore networks. In contrast, low-energy, fine-grained facies experienced a destructive pathway dominated by intense compaction and cementation, leading to the deterioration of pore structure. The petrophysical properties of the deep reservoirs are primarily governed by the three-dimensional connectivity and spatial distribution of effective “pore-throat assemblages” composed of dominant throats. Accordingly, a “binary” pore structure development pattern is established for the deep tight sandstone reservoirs in the study area. This pattern posits that the reservoir space is heterogeneously composed of a minority of connected “effective percolation assemblages” and a majority of isolated “ineffective assemblages”. Full article

To address runoff forecasting inaccuracies caused by data gaps in reservoir operations, this paper proposes a collaborative modeling framework integrating deep learning, signal decomposition, uncertainty quantification, and transfer learning. Validated on the Wei River (source basin) and Yongding River (target basin) with similar hydrological characteristics, the framework first constructs a Pyraformer-BiLSTM-LSS point forecasting model to enhance characterization of non-stationary runoff sequences. Then, the BLSO-VMD optimization decomposition technique filters and reconstructs forecasting noise, improving model robustness. Subsequently, a probabilistic interval forecasting model is developed via multi-task learning to reliably quantify uncertainty. To tackle data scarcity in the target domain, a “decomposition–reconstruction–transfer” learning mechanism transfers model knowledge from the source domain to the target domain. Results show that the framework achieves excellent performance in the source domain and successfully transfers to the data-scarce target domain, significantly enhancing the accuracy and stability of both point and interval forecasts. By establishing a collaborative modeling framework combining transfer learning and multi-task learning, along with an adaptive signal decomposition method based on BLSO and a multi-scale deep learning model, this study effectively addresses the challenges of accuracy and reliability in runoff forecasting for data-scarce basins. It provides a transferable and scalable technical pathway for runoff simulation and reservoir operation in hydrologically underserved regions, supporting sustainable water resource management and ecological protection. Full article

Croatia’s diverse agroecological zones, from Mediterranean coastal areas to continental lowlands, enable the cultivation of a broad portfolio of traditional fruit species that contribute simultaneously to biodiversity conservation, rural livelihoods, and the development of value-added food and beverage products. This review compiles and harmonizes evidence on six economically and culturally relevant crops and product chains—grapevine and wine, apple, pear, quince, sour cherry, mulberry, and plum with the traditional spirit šljivovica—focusing on genetic resources and cultivar diversity, agronomic and environmental performance, bioactive composition and potential health relevance, processing routes and by-product valorization, and the socio-economic roles of geographical indications, gastronomy, and tourism. Across species, the literature highlights recurring sustainability levers: safeguarding indigenous and old cultivars as reservoirs of adaptive traits under climate change; reducing chemical inputs through cultivar choice, organic and low-input systems, cover crops, and resistant genotypes; strengthening circularity by converting pomace and other residues into spirits, vinegars, functional ingredients, feed, compost, or energy carriers; and increasing rural value capture through branding, protected origin schemes, and experiential tourism. At the same time, production systems face shared constraints, including fragmentation of holdings, labour shortages, phytosanitary pressures, and the need to optimize processing technologies to preserve sensory and bioactive quality while meeting safety and regulatory requirements. By integrating crop-specific evidence with cross-cutting sustainability themes, this review outlines a coherent framework for positioning traditional Croatian fruit resources and their derived products within contemporary sustainable food system transitions. Full article

The hepatitis B (HBV), C (HCV), and D (HDV) viruses remain a major public health burden. Occult HBV infection (OBI) represents a hidden reservoir with clinical and epidemiological significance, yet its prevalence in Northwest Russia is unknown. We aimed to comprehensively assess the serological and molecular epidemiology of HBV, HCV, and HDV in St. Petersburg and the Leningrad region. Methods. In this cross-sectional study, 6773 apparently healthy volunteers were enrolled. Plasma samples were tested for hepatitis B surface antigen (HBsAg), antibodies to HBV core antigen (anti-HBc), antibodies to HBsAg (anti-HBs), antibodies to HCV (anti-HCV), and antibodies to HDV (anti-HDV) by ELISA. All anti-HCV- and anti-HDV-positive samples were tested for HCV RNA and HDV RNA by real-time PCR. All samples were tested for HBV DNA using a highly sensitive in-house nested real-time PCR assay (detection limit: 5 IU/mL). All “HBV DNA-positive, HBsAg-negative” cases confirmed by two independent extractions were classified as OBI. Vaccination status, self-reported history, and iatrogenic interventions were recorded. Results. Overall seroprevalence values were: HBsAg 1.7%; anti-HBc 11.3%; anti-HBs 43.0%; anti-HCV 1.9%; and anti-HDV 0.6%. Anti-HBc increased sharply with age (3.1% in children to 26.4% in the elderly, p < 0.0001), while anti-HBs declined (69.9% to 29.8%, p < 0.0001). HBV DNA was detected in 118 participants (1.7%). Of these, only 73 individuals (1.1%) were HBsAg-positive, while the remaining 45 participants (0.7%) had undetectable HBsAg, meeting the criteria for OBI. OBI was detected across all age groups, including children. Serological profiling of OBI cases revealed that 57.8% lacked both anti-HBc and anti-HBs, 35.6% had isolated anti-HBs, 2.2% had isolated anti-HBc, and 4.4% had both antibodies. HCV RNA was detected in 15.0% of anti-HCV-positive individuals (all adults). No HDV RNA was detected. Self-reported history underestimated true infection rates: 1.4% of those denying HBV infection were HBsAg-positive and 10.6% were anti-HBc-positive. Among those denying HCV infection, 1.4% were anti-HCV-positive. Vaccination coverage was 70.8%, declining from 90.9% in children to 39.0% in the elderly (p < 0.0001). Among vaccinated individuals, 48.0% lacked protective anti-HBs (<10.0 mIU/mL). Conclusions. This comprehensive serological and molecular study in Northwest Russia is the first to combine population-level serology with molecular detection of HBV, HCV, and HDV, including OBI in this region, and reveals that OBI accounts for a substantial proportion (38%) of all active HBV infections and is strongly associated with a history of iatrogenic interventions. The presence of OBI across all age groups, including children, shows that HBsAg screening alone substantially underestimates the true HBV burden. High rates of unrecognized infection and waning vaccine-induced immunity, highlight critical gaps in current surveillance. These findings provide an evidence-based rationale for integrating molecular testing into screening algorithms and for considering booster vaccination strategies to achieve viral hepatitis elimination goals. Full article

A critical challenge in coalbed methane (CBM) extraction is the severe formation damage induced by conventional foam fracturing fluids, primarily through polymer retention and hydrogen bond disruption within the microporous matrix. This study presents a molecularly engineered, low-damage foam fracturing fluid that leverages synergistic nanoparticle–surfactant interactions to construct a robust interfacial pseudo-gel network, thereby decoupling effective fracture stimulation from adverse geochemical damage. The primary novelties of this work are threefold: (i) establishing a direct, quantitative cause-and-effect relationship between molecular interfacial architecture and reservoir protection, (ii) proposing a comprehensive “interfacial control” design paradigm that engineers viscoelasticity at the gas–liquid interface rather than through bulk polymer gelation, and (iii) demonstrating the complete decoupling of foam stability from formation damage in a polymer-free system. A systematic optimization methodology was employed: initial foaming agents were screened via the Waring Blender method, evaluating foam volume, half-life, and a derived comprehensive index; subsequently, synergistic binary surfactant mixtures and foam stabilizers were assessed to formulate the final systems. An optimized formulation, designated Foam System I (0.5 wt.% fluorosurfactant FK + 0.5 wt.% nano-silica RX + 2.0 wt.% KCl), demonstrated exceptional foam quality (Γ = 77.1 ± 1.5%) and kinetic stability (T₁/₂ > 350 s). Rheological characterization confirmed shear-thinning behavior conforming to the Herschel–Bulkley model (n = 0.38–0.42, R² > 0.98) and a structural recovery of 92.5 ± 2.1%—comparable to crosslinked polymer gels but achieved without any bulk viscosifier. Core flood analyses revealed that Foam System I induced a permeability damage of only 12.75 ± 1.8%, representing a 55–75% reduction compared to polyethylene glycol (PEG)-stabilized reference fluids (28.36–51.91%). X-ray photoelectron spectroscopy (XPS) correlated this enhanced reservoir compatibility with an 18.0 ± 2.0% suppression of oxygen-containing functional group adsorption, attributed to the steric hindrance conferred by the fluorinated hydrophobic moieties. This work establishes an “interfacial control” paradigm wherein gel-like stabilization for proppant transport is achieved via interfacial viscoelasticity rather than bulk polymer gelation, thereby directly addressing the critical imperative to harmonize fracture conductivity with reservoir protection in unconventional energy development. The findings are validated for shallow CBM reservoir conditions (25–35 °C), with extension to higher-temperature formations identified as a priority for future investigation. Full article