Search Results (64)

Camels have been essential to human survival and development across the arid Central Asian steppes, particularly in Kazakhstan, where the breeding of one-humped and two-humped camels is a longstanding tradition supporting the nomadic lifestyle. This study aimed to assess the genetic diversity and population structure of these camels across their distribution range in Kazakhstan. Blood samples from 100 individuals were collected from five locations, Almaty (ALA), Atyrau (ATR), Shymkent (SHK), Kyzylorda (KZL), and Taraz (TRZ), and genotyped using 17 microsatellite markers. All loci were polymorphic, with a mean observed heterozygosity of 0.707 in C. dromedarius and 0.643 in C. bactrianus. The highest expected heterozygosity (He = 0.939) was observed at VOLP67 in C. bactrianus and at VOLP03 in C. dromedarius. Genetic differentiation was low (FST = 0.021), indicating a weak population structure between the two species with substantial gene flow (Nm = 19.972). The hybrid analysis identified 31% hybrids, including F1, F2, and backcrosses, with the highest frequencies in KZL and TRZ, moderate frequencies in ATR, and lowest frequencies in SHK and ALA. These patterns, consistent with STRUCTURE clustering, reflect widespread but regionally variable hybridization. The phylogenetic analysis revealed three clades, separating Bactrian camels (ALA), dromedaries (SHK), and a hybrid group (ATR, KZL, and TRZ). These findings enhance our understanding of the genetic diversity of Kazakhstan’s camels and support effective conservation, breeding strategies, and genotyping applications in camel husbandry. Full article

Background: The prevalence of overweight and obesity has increased over the last three decades, becoming a major public health concern. This issue is particularly pressing in terms of the impact it has on the population of the Kingdom of Saudi Arabia (KSA). Thus, the aim of this research was to explore the nutritional and lifestyle habits of youth in the Al-Ahsa region of the KSA. Methods: This cross-sectional study included a total of 1270 secondary-school boys and girls (15–19 years) from all five of the Al-Ahsa Governorate districts. BMI and waist circumference measurements were recorded using anthropometric measurements, and the lifestyle–Arab Teens Lifestyle Survey (ATLS) was used to measure sedentary and eating habits. Results: Chi-square analysis revealed that a higher proportion of females (90.68%) than males (79.18%) exceeded two hours of daily sedentary time. Frequent sugary drink consumption (>3 times per week) was similar in males (67.40%) and females (66.11%). Students from different geographical locations showed significant differences in exceeding cut-off scores for fast food (χ² = 24.503, p < 0.001), cakes/doughnuts (χ² = 8.414, p = 0.015), sweets/candy (χ² = 19.613, p < 0.001), and energy drinks (χ² = 21.650, p < 0.001). Conclusions: Al-Ahsa is the largest governorate in the KSA. It has some of the largest health risks regarding obesity and poor lifestyle habits. This study highlights the major need for future research and policy interventions. Full article

Physical activity (PA) and body composition are important lifestyle factors that influence public health. Research suggests that DNA regions (CpG site locations) are differentially methylated in a physically active population. This meta-analysis aimed to identify CpG sites associated with various levels of PA and associated metabolic pathways. The meta-analysis followed PRISMA guidelines using PubMed, SportDISCUS, Embase, Scopus, Cochrane and Web of Science. Epigenomic analyses performed on DNA of participants with no underlying health conditions were included. Articles were screened using Rayyan AI and extracted CpG sites, and their location were confirmed using the EWAS catalogue. Six studies comprising 770 subjects were included in this meta-analysis. The meta-analysis was performed on clinical metrics extracted from the six studies and showed that BMI, blood pressure, insulin and glucose testing are significantly improved upon PA intervention. Amongst the included studies, a total of 257 CpG sites were differentially methylated in physically active participants, with 134 CpGs located in 92 genes associated with obesity-related pathways. The identified differentially methylated genes either belonged to the lipid metabolism or insulin signalling pathway. The genes which were differentially regulated in multiple tissue types and studies are JAZF1 (insulin signalling, and lipid and carbohydrate metabolism pathways) and NAV1 (mTOR signalling pathway). In conclusion, the current epigenomic meta-analysis showed that PA levels induce differential DNA methylation signatures on genes that affect metabolism. To understand the positive molecular effects of PA, further research on the above candidate genes needs to be conducted amongst various levels of a physically active population. Full article

Background/Objectives: Research considering objectively measured moderate-to-vigorous physical activity (MVPA) and sedentary behaviors (SB) and health outcomes among urban children has produced equivocal findings. Therefore, this study was designed (i) to compare MVPA and SB of urban and non-urban children and (ii) to analyze associations between the risk of overweight and MVPA of children by their degree of urbanization. Methods: The sample comprised 389 children (195 girls) aged 6 to 10 years. Measurements for height and weight were taken, followed by the calculation of body mass index (BMI). A motion sensor was employed to gather data on MVPA and SB for 7 days. Urban regions were characterized as areas with a population density exceeding 500 inhabitants per square kilometer or having a total population greater than 50,000. ANOVAs, partial correlations, and logistic regression analysis were used, controlling for potential confounders. Results: After controlling for wearing time and sex, urban children were significantly less active (lower MVPA) than non-urban peers [i.e., urban: 48 min/day vs. 51 min/day (non-urban)]. On the other hand, urban children spent significantly more time on SB than their non-urban counterparts on the weekend and during all assessed days. MVPA was significantly associated with the risk of being overweight at the weekend for both urban and non-urban children. Inspection of the final regression model indicated that urban children who engaged in sedentary behavior for extended periods were at a higher risk of being categorized as overweight. Conclusions: Findings revealed the association between MVPA and the risk of overweight on weekdays was just significant for urban children. The location where a child lives significantly influences their weight status, and therefore, community-based programs [at schools (PE and recess) and after-school (organized sports)] should be developed that encourage active lifestyles tailored to urban environments. Full article

Climate change and geological shifts were pivotal in the survival and development of ancient human societies, especially in densely populated regions like the middle and lower Lishui River Basin. This study explored the dynamic interactions between ancient human cultures and the region’s natural environment, using field research alongside geological, geomorphological, and archaeological data spanning from the late Paleolithic to the Neolithic periods. Our findings showed that prehistoric sites in the middle and lower Lishui River Basin were primarily located in the low hilly areas surrounding the lower basin during the Paleolithic era, a pattern shaped by the region’s geomorphology. Early human settlements were strategically positioned near the Lishui River, offering access to vital resources while minimizing flood risk. These locations provided flat terrain, abundant vegetation, and materials for tool-making, factors that supported a hunting and gathering lifestyle. As the coldest phase of the last glacial period approached, the transition from the Paleolithic to the Neolithic eras marked a period of significant behavioral adaptation. In response to the harsher environment, humans began settling on lower terraces and miniaturizing their stone tools, signaling a shift to more specialized hunting techniques. This adaptability and resilience marked the refinement of hunting economies during this period. With the onset of the Holocene epoch and a warmer climate, conditions for human habitation became more favorable. Societies began migrating from the hills to the fertile lower Lishui River Basin, heralding the Neolithic period. This era saw the emergence of settlements and the onset of early rice cultivation, marking the transition from a hunting–gathering economy to one centered on agriculture. By the Daxi period, these settlements had expanded, extending their influence throughout the region. In conclusion, this study underscores the critical roles of climate change and geological features in shaping human settlement patterns, economic activities, and cultural evolution in the middle and lower Lishui River Basin. Our findings offer valuable insights into the evolutionary processes of ancient human cultures in the region and provide a foundation to understand future challenges in sustainable development. Full article

Feline cancer is increasingly recognized as a major cause of mortality, yet data on tumor prevalence and behavior in cats, particularly in non-Western regions, remain limited. This study analyzed a decade of feline tumor data in Korea from 2012 to 2022, focusing on age, breed, and anatomical location as predictors of malignancy. Data were collected from 683 cats, with regression analysis applied to determine significant associations. Older cats exhibited a markedly higher risk of malignancy, particularly in mast cell and mammary tumors. Tumors in the mammary gland and alimentary tract had malignancy rates exceeding 90%, underscoring the need for early detection in these regions. Interestingly, squamous cell carcinoma was rare in the skin, in stark contrast to Western studies, likely reflecting differences in environmental exposure. While breed was not a statistically significant predictor, certain breeds, including Persians and Russian Blues, showed a higher frequency of malignancy. These findings highlight the importance of regional tumor research in cats and the need for larger, multicenter datasets that incorporate environmental, genetic, and lifestyle factors. Understanding these influences will help refine veterinary care and improve cancer treatment outcomes in feline populations. Full article

Current societal shifts, including increased mobility, advances in information technologies, and diverse lifestyles, are driving postmodern housing arrangements that prioritize mobility and flexibility. The traditional view of housing as fixed and stable is evolving to encompass temporary forms, including multi-locality, which are often underestimated due to measurement challenges. Recognizing and integrating the potential of transient populations into urban societies is crucial to enhancing community cohesion and reducing social fragmentation. Therefore, the development of rootedness, emotional connection, and spatial identity should be analyzed in the context of temporary housing practices. Data collected through a representative survey in two major German urban regions in 2023 enable the validation of a four-dimensional place attachment scale, which includes the dimensions of place identity, place dependence, ambient bonding, and social bonding. Each dimension includes distinct elements of place-making constructions. The classification of four tempo-local housing types serves as the foundation for measuring how the temporal and spatial structure of housing arrangements influences emotional attachment to one’s place of residence. Interestingly, spatial mobility, when paired with temporal stability in each location, can cultivate deep connections, revealing an often-overlooked potential within this group. The results emphasize the significance of incorporating spatio-temporal dimensions of housing to foster inclusive, comprehensive, and diverse urban development. Full article

The KALRN gene (encoding kalirin) has been implicated in several neuropsychiatric and neurodegenerative disorders. However, genetic evidence supporting this implication is limited and targeted epigenetic analyses are lacking. Here, we tested associations between epigenetic variation in KALRN and interindividual variation in depressive symptoms (PHQ9) and cognitive (MoCA) performance, in an Italian population cohort (N = 2409; mean (SD) age: 67 (9) years; 55% women). First, we analyzed the candidate region chr3:124584826–124584886 (hg38), within the KALRN promoter, through pyrosequencing of 1385 samples. Then, we widened the investigated region by analyzing 137 CpGs annotated to the whole gene, rescued from epigenome-wide (Illumina EPIC) data from 1024 independent samples from the same cohort. These were tested through stepwise regression models adjusted for age, sex, circulating leukocytes fractions, education, prevalent health conditions and lifestyles. We observed no statistically significant associations with methylation levels in the three CpGs tested through pyrosequencing, or in the gene-wide association analysis with MoCA score. However, we observed a statistically significant association between PHQ9 and cg13549966 (chr3:124106738; β (Standard Error) = 0.28 (0.08), Bonferroni-corrected p = 0.025), located close to the transcription start site of the gene. This association was driven by a polychoric factor tagging somatic depressive symptoms (β (SE) = 0.127 (0.064), p = 0.048). This evidence underscores the importance of studying epigenetic variation within the KALRN gene and the role that it may play in brain diseases, particularly in atypical depression, which is often characterized by somatic symptoms. Full article

The prevalence and incidence of NAFLD is rising due to the obesity pandemic, caused by the widespread availability of ultra-processed foods and the decrease of physical activity. Factors such as socioeconomic status (SES), ethnicity and geographical location are associated with NAFLD, with lower SES correlating with higher incidence, particularly in regions like America or Europe. Beside the quality of food, the quantity also plays a crucial role. The World Health Organization (WHO) recommends a Mediterranean diet with a balanced energy intake. Since no hard medical treatment is available for NAFLD, lifestyle adjustments are key. Patient empowerment by providing relevant information and co-ownership of the therapy will increase the implementation rate and enhance the quality of medical follow-up and medication adherence, as studies report a good adherence to medication among patients who are well-aware of the severity of their disease. Regarding sustainability, patients with NAFLD have a high load of ambulatory follow-up, which, since the COVID-19 pandemic, can be partially provided by teleconsulting. Both patients’ lifestyle modifications and healthcare practitioners’ therapeutical strategy can decrease the carbon footprint. Full article

Given the financial demands of attending college, the transition to new living situations, abrupt changes in social support, and overall lifestyle adjustments, college students are at an increased risk of food insecurity (FI) compared to the general population. Collegiate athletes experience an even greater risk of FI as a result of greater time commitments and energy demands associated with their sports. This heightened vulnerability poses a tremendous threat to student-athletes’ academic and athletic achievements. This study aims to address the prevalence and primary determinants of FI among collegiate athletes while providing potential solutions to navigate and alleviate the effects of diminished food security among this demographic. To address these aims, a total of 18 articles were selected from both peer-reviewed and gray literature. The U.S. Household Food Security Survey Module (US-HFSSM) survey tools were predominantly utilized across universities throughout the United States to gather data on FI. Student-athletes reported experiencing FI across various regions of the United States, including universities in the northeastern states (n = 5), the southwest region (n = 3), the southeast region (n = 3), the northwest (n = 1), and the Midwest (n = 1). Overall, FI prevalence rates ranged from 9.9% to 65%, and the most significant contributors included limited financial resources, time management, meal plans, and housing location/amenities. These findings highlight a need for screening, education, and interventions to address FI among collegiate athletes. Full article

Cancer patients undergoing major interventions face numerous challenges, including the adverse effects of cancer and the side effects of treatment. Cancer rehabilitation is vital in ensuring cancer patients have the support they need to maximise treatment outcomes and minimise treatment-related side effects and symptoms. The Active Together service is a multi-modal rehabilitation service designed to address critical support gaps for cancer patients. The service is located and provided in Sheffield, UK, an area with higher cancer incidence and mortality rates than the national average. The service aligns with local and regional cancer care objectives and aims to improve the clinical and quality-of-life outcomes of cancer patients by using lifestyle behaviour-change techniques to address their physical, nutritional, and psychological needs. This paper describes the design and initial implementation of the Active Together service, highlighting its potential to support and benefit cancer patients. Full article

The integrated development of green innovation and digital economy is crucial for addressing global climate change, promoting the application and popularization of digital technologies in the green sector, and achieving efficient, intelligent, and sustainable economic development and lifestyles. Using panel data from China’s listed enterprises from 2003 to 2021, this study examines the impacts of environmental regulations on green innovation in the low-carbon city pilot policy (LCCPP) context, which serves as a quasi-natural experiment in the digital economy. The results show that LCCPP effectively enhances enterprises’ green innovation by increasing enterprises’ environmental and research and development investments, and boosting digital transformation. Heterogeneity analysis reveals that the promotion effect of LCCPP on green innovation is highly pronounced for enterprises in the eastern region, for those with low financial constraints, and for technology-intensive enterprises. This effect is closely and positively correlated with the digital economy level in the regions where the enterprises are located. This study provides new empirical evidence for the relationship between environmental regulations and enterprises’ green innovation and discusses policy support for addressing global climate change challenges through environmental regulations in the digital economy context, thereby promoting global sustainable development. Full article

Background: Lifestyle interventions halt the progression of prediabetes to frank type 2 diabetes (T2D). However, the feasibility of a diabetes prevention program promoting tailored interventions on a national scale and conducted by primary care physicians is unclear. Methods: General practitioners located in ten different regions throughout Italy enrolled random subjects without known metabolic diseases to identify individuals with prediabetes and prescribe them an intervention based on physical activity. Using a simple stepwise approach, people referring to their primary care physician for any reason were screened for their diabetes risk with a web-based app of the Findrisc questionnaire. Those at risk for T2D, i.e., with a Findrisc score >9, were invited to come back after overnight fasting to measure fasting glycaemia (FG). Those with 100 ≤ FG < 126 mg/dL were considered as people with prediabetes and compiled the Physical Activity Readiness Questionnaire (PAR-Q) to then receive a personalised prescription of physical activity. Results: Overall, 5928 people were enrolled and compiled the questionnaire. Of these, 2895 (48.8%) were at risk for T2D. Among these, FG was measured in 2168 subjects (participation rate 75%). The numbers of individuals with undetected prediabetes and T2D according to FG were 755 and 79 (34.8% and 3.6% of those assessing FG), respectively. Of the 755 subjects in the prediabetes range, 739 compiled the PAR-Q and started a personalised program of physical activity (participation rate 97%). Physicians involved in the study reported a mean of 6 min to perform the screening. Conclusions: Overall, these data suggest the feasibility of a national diabetes prevention program developed by general practitioners using a simple stepwise approach starting from a web app to intercept individuals with prediabetes. Full article

In the present study, the mycobiomes of two soils with different ecological conditions located in Benin (West Africa) were investigated by environmental sequencing (Illumina MiSeq) of the ITS2-region of ribosomal DNA to gain information about the influence of pedological stratification on fungal diversity. For each soil depth and horizon, fungal diversity and community composition were analyzed as well as the potential impact of site characteristics, like vegetation, on these traits. The retrieved sequences revealed in all their replicates high similarities between fungal communities of samples from the same site and soil horizon, but differed within one site in their horizons. It was possible to assign a saprotrophic, symbiotrophic, or parasitic lifestyle to 24% of the recorded fungal mOTUs. Plant parasites were found in all samples in similar proportions. The presence of ectomycorrhizal fungi in one site could be linked to the presence of ectomycorrhizal trees. Overall, it was observed that fungal diversity decreased with increasing depth if only one horizon was present, whereas a deeper horizon present at one site contained communities with a distinct composition regarding the taxonomical affiliations and lifestyles of the fungi found compared to the upper layer. Hence, soil horizonation seems to drive differences in the composition of fungal communities, and should be regarded with more attention when analyzing soil mycobiomes. Full article

Human papillomavirus (HPV) is implicated in over 90% of cervical cancer cases, with factors like regional variability, HPV genotype, the population studied, HPV vaccination status, and anatomical sample collection location influencing the prevalence and pathology of HPV-induced cancer. HPV-16 and -18 are mainly responsible for the progression of several cancers, including cervix, anus, vagina, penis, vulva, and oropharynx. The oncogenic ability of HPV is not only sufficient for the progression of malignancy, but also for other tumor-generating steps required for the production of invasive cancer, such as coinfection with other viruses, lifestyle factors such as high parity, smoking, tobacco chewing, use of contraceptives for a long time, and immune responses such as stimulation of chronic stromal inflammation and immune deviation in the tumor microenvironment. Viral evasion from immunosurveillance also supports viral persistence, and virus-like particle-based prophylactic vaccines have been licensed, which are effective against high-risk HPV types. In addition, vaccination awareness programs and preventive strategies could help reduce the rate and incidence of HPV infection. In this review, we emphasize HPV infection and its role in cancer progression, molecular and immunopathogenesis, host immune response, immune evasion by HPV, vaccination, and preventive schemes battling HPV infection and HPV-related cancers. Full article