Search Results (13)

Conservation managers increasingly employ reinforcement techniques to bolster declining populations by reintroducing non-wild individuals born in captivity into natural habitats, but success rates remain modest. In this study, the success is evaluated of reinforcement efforts using satellite tracking and field observation data collected between 2010 and 2021. It focuses on 13 non-wild individuals, as follows: seven red-crowned cranes Grus japonensis, two white-naped cranes Antigone vipio, and four demoiselle cranes Anthropoides virgo, as well as five wild individuals including two red-crowned cranes and three white-naped cranes. The assessment criteria included survival, movement, and reproduction, utilizing a comprehensive scoring method. The scoring process indicates that more timely field observation records and the movement pattern scoring combining models and trajectories can improve the accuracy of estimation. From the results, although wild individuals generally achieve higher scores across these metrics, statistical differences were not significant possibly due to limited sample size. Notably, non-wild individuals frequently displayed residence, nomadic, or abnormal migration. In addition, field observations underscored the benefits of pairing non-wild individuals with their wild counterparts to enhance migration success. So in order to enhance migration success, it is advisable to release non-wild individuals approaching sexual maturity in proximity to wild subadult flocks during the breeding or summering periods. Additionally, during the overwintering phase, these individuals should be released in areas where wild populations are concentrated. Full article

Red-crowned cranes (Grus japonensis) consist of two distinct groups: the continental population and the island population. The island population, localized in Hokkaido, Japan, exhibits very low genetic diversity due to its rapid recovery from the brink of extinction. Our previous research in 2018 highlighted a possible mating between a male from the continental population, with the Gj5 haplotype, and a female from the island population, with the Gj2 haplotype, at Hitominuma Sawmp shore in northern Hokkaido. The present study attempted to unravel the distribution of their offspring by examining the major histocompatibility complex (MHC) of this mixed breeding pair compared with samples collected from cranes in northern and southeastern Hokkaido between 2008 and 2022. The analysis identified 55 MHC types, including 10 known types in a dataset of 89 crane samples, based on amino acid sequences. A total of 58 MHC types were recognized, based on nucleotide sequences, as there were many cases in which the same amino acid sequence had different nucleotide sequences. The five DNA types of MHC in the Hitominuma Swamp male were predominantly identified in eight cranes from northern Hokkaido and one chick from southeastern Hokkaido. In addition, population genetic analysis, based on insertion/deletion (InDel) polymorphisms, indicates distinct population differentiation between the northern and southeastern regions of Hokkaido. These results suggest that genetic contributions from the continental red-crowned crane population have already been integrated into the Hokkaido populations, with a more pronounced influence in northern Hokkaido. Full article

The wild populations of red-crowned cranes (Grus japonensis) in west China are gradually decreasing, necessitating the optimization of reintroduction measures. This study used 16S rRNA high-throughput sequencing technology to compare the gut microbiota communities of cranes living in two modes (captive and semi-free-range) before their reintroduction in Zhalong National Nature Reserve, Heilongjiang Province, China. The results showed that Proteobacteria (74.39%) and Firmicutes (25.29%) were the dominant gut bacterial phyla inhabiting these cranes. Significant differences were found in the gut microbiota community composition between semi-free-range and captive cranes (p < 0.01). Psychrobacter, Sporosarcina, and Lactococcus were significantly enriched in captive cranes (p < 0.05), while Pseudomonadaceae_Pseudomonas, Pantoea, Lysobacter, and Enterobacteriaceae_Pseudomonas were more abundant in semi-free-range cranes (p < 0.05). The functions and community structure of gut microbiota were affected by feeding patterns (p < 0.05). The metabolic pathways of ethylbenzene degradation, PPAR signaling pathway, betalain biosynthesis, systemic lupus erythematosus, and shigellosis were up-regulated in semi-free-range cranes (p < 0.05). Full article

Total DNA extracts from the intestinal contents of 60 flying red-crowned cranes (juveniles, subadults and adults) found dead in 2006–2021, and the feces of 25 chicks collected in June and July of 2016–2018, were used for PCR reactions with primers specific for 16 crops, followed by high-throughput sequencing. The most predominant crop detected was corn in adult and subadult cranes (61.7%). Other grains (barley, wheat, soybean) (5.0–8.3%) and vegetables (tomatoes, Chinese cabbage, etc.) (1.7–6.7%) were also detected in flying cranes. Surprisingly, some of the detected crops were not grown in the Kushiro and Nemuro regions. There was no significant difference in crop intake status in winter and that in other seasons for most of the crops. Corn (28.0%), soybeans (8.0%), wheat and beet (4.0%) were detected in crane chicks in summer, though the detection rates were generally lower than those in flying cranes. Alfalfa, which is not grown in eastern Hokkaido but is used in some cattle feed, was detected in some cranes. Rice, buckwheat, adzuki beans, common beans, potatoes and carrots were not detected at any life stage, indicating the preferences of red-crowned cranes. The results suggest that red-crowned cranes in Hokkaido are dependent on dairy farmers for their feed supply. Full article

The National Yancheng Rare Birds Nature Reserve is a vitally important staging habitat for the wild population of red-crowned cranes (Grus japonensis) in China. The population relies on local high-protein food sources, such as fish juveniles, to fuel their migratory journeys. However, little is known about the ecology of the fish larvae and juveniles that migrate to the inshore area via tidal rhythm in Subei Shoal, which is adjacent to the reserve. Therefore, we used a fixed study station (32°55′1.2″ N, 121°19′58.8″ E) to conduct a continuous 15-day ichthyoplankton survey at 2 h intervals beginning at 05:00 on 25 April and ending at 03:00 on 10 May 2019. We identified the tidal variations in the number of fish larvae and juveniles and the number at various developmental stages and assessed how they were related to environmental variables such as sea surface temperature, salinity, turbidity, and tidal height in the Dafeng Sea area of Subei Shoal. We found that the number of species and larval individuals were highest and lowest, respectively, at the highest and lowest tidal height, and they obviously increased and decreased with the rising and ebb tide, respectively. Our findings indicate that the variation in numbers of the larvae and juveniles depends on species and developmental stage. The species Acanthogobius ommaturus, Pholis fangi, Cynoglossus joyneri, Liza haematocheila, and Lateolabrax japonicus and the total number of larvae were most influenced by tidal height. These results provide a better understanding of the habitat of prey species of the red-crowned crane wild population as well as scientific data that can be applied to manage the wild population in the reserve sustainably. Full article

In order to effectively protect the habitat of cranes, this study constructs an indicator evaluation system based on the ecology–economy–society complex system and adopts the comprehensive “entropy weight method and analytic hierarchy process” evaluation model and coupled coordination model to scientifically measure the degree of coordinated development of the EES system in Yancheng. Further, a negative binomial regression model based on LASSO was used to analyze the key factors affecting the habitat quality of red-crowned cranes, and a support vector regression model was used to predict the population size of the cranes. The results show that the degree of the coordinated development of the EES system exhibited a fluctuating upward phenomenon, and the population size of the cranes also had a similar evolutionary trend, which indicates that the interaction between the two was significant and that the degree of the coordinated development of the system had a positive impact on the quality of the habitat of the cranes. Three types of ecological indicators (normalized difference vegetation index, annual precipitation, and soil erosion area) and three types of social indicators (natural growth rate, rural Engel coefficient, and public library collection) are the key factors affecting the population size of the cranes. The prediction results of the support vector regression model showed that the population of the cranes showed a fluctuating upward trend during the prediction interval, with a maximum of 952 cranes and an overall growth rate of 69.70%. The population size of the cranes is related to human social activities and the surrounding ecological environment, and the main reason for the decline in the population size of the cranes is the destruction of the local vegetation cover by the rapidly growing population and frequent human activities. Therefore, to improve the habitat quality of the cranes, local government departments need to strengthen the publicity of wildlife conservation, reduce agricultural land reclamation and pesticide pollution, and promote the coordinated development of the EES system in the Yancheng area. Full article

Red-crowned crane Grus japonensis is an endangered species in two separate populations: the mainland population in the Eurasian continent and the island population in eastern Hokkaido, Japan. We found 11 insertion/deletion (InDel) markers in the genome of the red-crowned crane and designed primer sets across these InDels that can be analyzed with conventional agarose gel electrophoresis. Sixty-six samples of whole blood and skeletal muscle obtained from red-crowned cranes, including 12 families in eastern Hokkaido from 1994 to 2021, showed different patterns in gel images of 11 InDel PCR reactions except for two pairs. The combined non-exclusion probability of the 11 markers indicates that individuals can be determined with a probability of 99.9%. In 39 non-relative chicks, the expected heterozygosity (He) was 0.316, suggesting low genetic diversity. This might not be caused by high levels of inbreeding since the average FIS was not significantly different from zero (0.095, p = 0.075). The results suggest that the 11 InDel primer sets can be used for fairly accurate individual identification as well as genetic population analyses in red-crowned cranes in the island population. Full article

Gut microbiota homeostasis is important for host health and well-being; however, drugs may affect the composition and function of the gut microbiota. Red-crowned cranes are a vulnerable species. Treatment of red-crowned cranes with avian influenza vaccines and anthelmintics has played pivotal roles in therapeutic management in zoos. To investigate the changes in the diversity and composition of gut microbiota after the avian influenza vaccine and anthelmintic treatment, we used 16S rRNA sequencing to obtain and compare the bacterial community composition before and after the treatment. The alpha diversity of the gut microbiota of red-crowned cranes decreased on the day of the treatment and then fluctuated over time. The composition of gut microbiota tended to be similar in the short term after the treatment, as supported by the beta diversity hierarchical cluster analysis. Only 3, 8, and 72 operational taxonomic units (OTUs) of the three individuals were shared among the five groups before and after treatment. The relative abundance of Firmicutes significantly increased to 99.04% ± 0.28% on the day of the treatment, in which the relative abundance of Lactobacillus was 93.33% ± 5.85%. KEGG pathways analysis indicated that the main function of the gut microbiota is involved in metabolism, and the present study indicates that the gut microbiota of red-crowned cranes is resilient to the avian influenza vaccine and anthelmintic, even disordered in the short term, and could recover over time. More individual experimentation and functional potential in metabolism are needed in the future to support animal disease control and optimal management in the zoo. Full article

In this work, an interval-parameter two-stage stochastic programming (IPTSP) model of water resources allocation was established for maximizing the restored habitat area of large, rare, and endangered water birds by adjusting the recommended scheme of water replenishment under different scenarios and constraints. The established model can efficiently deal with the uncertainties, such as the interval parameters and random variables, in the management system of water resources simultaneously. A case study was conducted in the Momoge National Nature Reserve (MNNR) in northeast China to maximize the restored habitat area of large, rare and endangered water birds based on limited water resources. According to the previous studies, a water area with a depth of 0–40 cm is a suitable habitat area in the MNNR for the Siberian crane, oriental stork, and red-crowned crane. The results of the present work show that the habitat area restored by water replenishment schemes under low, medium, and high flood flow scenarios after optimization increased in comparison to 13.36 × 10³ ha of the recommended scheme, with an increase of [0.62, 5.23], [1.49, 6.42], and [2.43, 7.17] × 10³ ha, respectively (the two numbers within each bracket represent the lower and upper bounds of the restored habitat areas). As a result, the carrying capacity of suitable habitat areas increased by [0.82, 6.88], [1.96, 8.45], and [3.21, 9.43] × 10³ birds, correspondingly. The restored wetland area of the project recommendation scheme was 34.23 × 10³ ha, and that of the optimal water replenishment schemes was [29.35, 41.01], [31.02, 44.13], and [33.88, 46.04] × 10³ ha, respectively under the three flood flow scenarios. The results reveal that the model constructed in this work realizes the optimization and adjustment of the initial scheme to an increased restored wetland and habitat area with an increase in the flow level. Here, the upper bound of the interval value mentioned above is significantly higher than the lower bound value, which indicates that a feasible decision space was provided for decision makers to optimize and adjust the recommended scheme on the basis of the actual situation. The model-optimized schemes significantly improved the utilization of limited water resources. The results of this study can provide valuable theoretical support for the restoration and protection of rare and endangered water bird habitats and planning and management of water resources. Full article

The red-crowned crane (Grus japonensis) is a vulnerable bird species. Mycotoxins are toxic substances produced by filamentous fungi and are considered as naturally unavoidable contaminants in animal feed. Our recent survey indicated that feeds designed for captive red-crowned cranes were contaminated with mycotoxins. This study was conducted to investigate the protective effects of the mycotoxin binder montmorillonite on the reproductive behavior, sex hormone levels, and egg quality of red-crowned cranes. Twelve pairs of G. japonensis were divided into four groups, and each group was fed one of the following: a selected diet (with extra low levels of mycotoxins), a regular diet, a selected diet with 0.5% montmorillonite added, or a regular diet with 0.5% montmorillonite added. Consumption of the regular diet decreased courtship and mating behaviors, testosterone concentration, egg weight, and shell thickness. However, feed supplementation with montmorillonite increased the courtship, mating behaviors and testosterone concentration during the pre-breeding period, as well as egg weight and shell thickness. These findings suggest that the addition of dietary montmorillonite is effective for controlling mycotoxins in the feed, resulting in improvements in reproductive behaviors, testosterone concentrations, and some egg quality parameters of the red-crowned crane. Full article

The study was the first attempt to identify the habitat use pattern of red-crowned cranes (Grus japonensis) around the demilitarized zone (DMZ) by overlapping coordinates with the land cover classification (LCC). Daily habitat use pattern was highly different (P = 0.000) between daytime (06:00–18:00) and nighttime (18:00–06:00). Cranes in Cheolwon used agricultural paddies more frequently in the daytime (P = 0.002), and forest areas at night and this indicated that cranes presumably use rice paddies for feeding and forests for resting, respectively. Cranes night time in Paju used wetlands more often than random expectation based on the available wetland surface area (P = 0.017). This indicated a different habitat use pattern between coastal (Paju) and inland (Cheolwon) areas. Securing agricultural paddies is important for providing crucial areas for feeding, and forests should be important for rest during the night time in Cheolwon, which support crane populations during their wintering migration in Korea. Full article

Cheorwon-gun is an important wintering area for the red-crowned crane (Grus japonensis). Although eco-tourism has been recently proposed as a means to stimulate the local economy, it may have adverse effects on the crane. We believe a science-based conservation plan is needed to mitigate these negative effects. To this end, our study had three objectives: (1) to analyze the red-crowned crane habitat and its suitability in Cheorwon-gun, using field surveys and habitat modeling; (2) to check the feasibility of alternative habitat patches across demilitarized zones (DMZs); and (3) to propose a conceptual diagram that minimizes habitat loss during development activities. We aim to quantify habitat suitability, the farmland area needed to support existing crane populations in wintertime, disturbance caused by human activities, and vehicular spatial patterns. These data could be used in spatial planning. The framework of this study and the process of making a conceptual diagram could be applied to other areas where there is a conflict between development and habitat conservation. Full article