Search Results (55)

Olfaction has been extensively studied in the yellow fever mosquito, Aedes aegypti. This species uses its sense of smell to find blood hosts and other resources, contributing to its impact as a vector for human pathogens. Two major families of protein-coding genes, the odorant receptors (Ors) and the ionotropic receptors (Irs), provide the mosquito with sensitivities to distinct classes of volatile compounds in the antennae. Individual tuning receptors in both families require co-receptors for functionality: Orco for all Ors, and Ir8a for many Irs, especially ones that are involved in carboxylic acid detection. In Drosophila melanogaster, disruptions of Orco or Ir8a impair receptor function, tuning receptor expression, and membrane localization, leading to general anosmia. We reasoned that Orco and Ir8a might also be important for coordinated chemosensory receptor expression in the antennal sensory neurons of Ae. aegypti. To test this, we performed RNAseq and differential expression analysis in wildtype versus Orco^−/− and Ir8a^−/− mutant adult female antennae. Our analyses revealed Or and Ir tuning receptors are broadly under-expressed in Orco^−/− mutants, while a subset of tuning Irs are under-expressed in Ir8a mutants. Other chemosensory and non-chemosensory genes are also dysregulated in these mutants. Furthermore, we identify differentially expressed transcription factors including homologs of the Drosophila melanogaster Mip120 gene. These data suggest a previously unknown pleiotropic role for the Orco and Ir8a co-receptors in the coordination of expression of chemosensory receptors within the antennae of Ae. aegypti by participating in a feedback loop involving amos and members of the MMB/dREAM complex. Full article

Background/Objectives: Total hip arthroplasty (THA) remains a widely utilized and effective intervention for patients with end-stage hip osteoarthritis. Although multiple surgical approaches and fixation techniques are available, their application in non-tertiary clinical settings is less frequently documented. This study primarily aims to provide an educational overview of stepwise THA procedures using intraoperative visual documentation, with a secondary, exploratory assessment of postoperative outcomes related to surgical approach and fixation strategy. Methods: A prospective observational study was conducted at Arad Clinical Emergency County Hospital between March 2023 and March 2024, involving 23 patients undergoing primary THA. Patients received either cemented or uncemented femoral components based on intraoperative bone quality. Procedures were documented using stepwise intraoperative photographs and postoperative radiographs. Recovery was assessed using the Harris Hip Score (HHS) and the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) at both six weeks and six months postoperatively. Results: Both lateral (Hardinge) and posterolateral approaches provided adequate exposure with reproducible results. Cemented implants allowed for immediate full weight-bearing and were preferred in elderly patients with poor bone quality, while uncemented components were used in younger patients with good bone density, requiring a delayed weight-bearing protocol. Functional scores improved in both groups between six weeks and six months. At six weeks, the mean HHS was 87.6 ± 6.2 and WOMAC 18.3 ± 4.8; by six months, these improved to 91.8 ± 5.1 and 12.7 ± 3.9, respectively. Cemented fixation demonstrated slightly better outcomes at both time points; however, intergroup differences remained below the Minimal Clinically Important Difference (MCID) thresholds. Conclusions: Tailored surgical approaches and fixation strategies, guided by intraoperative assessment, result in favorable short- and mid-term recovery profiles in THA. The integration of intraoperative visual documentation and patient-reported outcome measures (PROMs) enhances procedural transparency while supporting evidence-based decision-making and surgical training. Full article

Background/Objectives: Achieving optimal primary stability in cemented total hip arthroplasty remains a critical factor influencing long-term implant success. Variability in cementation techniques can significantly affect biomechanical performance, yet consensus on best practices is lacking. This study investigates the influence of cementation parameters on femoral stem fixation. Methods: This in vitro comparative study evaluated four cementation techniques—Classic (line-to-line), Press-Fit (undersized reaming), Overreaming (oversized reaming), and Valgus Malpositioning (15° deviation). An experimental model using standardized Polyurethane (PU) bone surrogates was developed. Mechanical testing assessed axial deformation and ultimate load capacity to failure. Results: The Press-Fit technique demonstrated significantly greater deformation (17.10 ± 0.89 mm) but a reduced load capacity (6317.47 ± 518.34 N) compared to the Classic approach. Overreaming and Valgus techniques both showed reduced mechanical performance, with Overreaming yielding the lowest structural integrity. Conclusions: Cement mantle thickness emerged as the primary determinant of biomechanical stability, surpassing the impact of implant positioning. While increased mantle thickness improves energy absorption, it may compromise ultimate strength. These findings underscore the importance of optimizing the cementation technique to balance flexibility and mechanical resistance, guiding surgical protocols toward improved implant longevity. This study introduces a novel integrative approach combining fluoroscopic assessment of cement mantle morphology with mechanical testing in a standardized model, providing new evidence on the relative influence of mantle thickness and implant malposition on femoral stem stability. Full article

The quality of metal products plays a crucial role in determining their overall performance, reliability and safety. Therefore, timely and effective detection of metal surface defects is of great significance. For this purpose, we present a densely connected network with multi-attention for metal surface defect segmentation, called MADC-Net. Firstly, we selected ResNet50 as the encoder due to its robust performance. To capture richer contextual information from the defect feature map, we designed a densely connected network and incorporated the multi-attention of a CESConv module, an efficient channel attention module (ECAM), and a simple attention module (SimAM) into the decoder. In addition, in the final stage of the decoder, we introduced a reconfigurable efficient attention module (REAM) to reduce redundant calculations and enhance the detection of complex defect structures. Finally, a series of comprehensive comparative and ablation experiments were conducted on the publicly available SD-saliency-900 dataset and our self-constructed Bearing dataset, all of which validated that our proposed method was effective and reliable in defect segmentation. Specifically, the Dice and Jaccard scores for the SD-saliency-900 dataset were 88.82% and 79.96%. In comparison, for the Bearing dataset, the Dice score was 78.24% and the Jaccard score was 64.74%. Full article

This paper presents a cost-effective approach for automated surface quality measurement in reamed bores. The study involved drilling 4000 holes into 42CrMo S4V steel, of which 3600 underwent subsequent reaming. Utilizing a CNC-controlled gantry coupled with a mobile roughness measurement device through a compliant mechanism, surface data of every bore were efficiently gathered and processed. Additionally, analytical methods are presented that extend beyond standardized, aggregated metrics. We propose the evaluation of retraction grooves by using autocovariance. In addition, the correlation between the phase position of the waviness profile and the positional deviation of the bore is analyzed. The position deviation is also associated with bending moments that occur during reaming using a sensory tool holder. Furthermore, a 360-degree surface scan is presented to visually inspect the retraction groove. This approach aims to enhance understanding of the reaming process, ultimately improving bore quality, reducing component rejects, and extending tool lifespan. Full article

Capacity fade in lithium-ion batteries (LIBs) poses challenges for various industries. Predicting and preventing this fade is crucial, and hybrid methods for estimating remaining useful life (RUL) have become prevalent and achieved significant advancements. In this paper, we introduce a hybrid voting ensemble that combines Gradient Boosting, Random Forest, and K-Nearest Neighbors to forecast the fading capacity trend and knee point. We conducted extensive experiments using the CALCE CS2 datasets. The results indicate that our proposed approach outperforms single deep learning methods for RUL prediction and accurately identifies the knee point. Beyond prediction, this innovative method can potentially be integrated into real-world applications for broader use. Full article

Hydraulic measures are widely used to improve coal seam permeability, but not all hydraulic measures have a positive effect on coal permeability in soft coal seams, and the permeability-enhancing effect of hydraulic measures in soft coal seams is not clear. To further study the permeability-enhancing mechanism of hydraulic measures and compare the effect of hydraulic punching and reaming in soft coal seams, this study takes Changping Mine, China, as its case study. A comparative analysis was conducted on the influence range and gas extraction effect of hydraulic reaming and punching on coal seam permeability enhancement. The following conclusions were mainly drawn: A mathematical calculation model was established for the strength and impact velocity of high-pressure water jet damage to the coal body, and the critical theoretical pressure threshold and jet velocity were obtained. During the implementation of hydraulic measures at the Changping Mine, the effective radius of hydraulic reaming is around 4.5 m, and the influence radius of hydraulic reaming is approximately 7.5 m; the effective radius of hydraulic punching is about 6.5 m, and the influence radius of hydraulic punching is approximately 7–9 m. The gas data from field monitoring show that hydraulic measures have significantly improved the extraction gas concentration and purity, and hydraulic punching has a more significant effect on enhancing permeability in soft coal seams. Full article

Objective: This study evaluated the effects of bony increased offset (BIO) and metallic augments (MAs) on primary reverse shoulder arthroplasty (RSA) baseplate stability in cadaveric specimens with variable bone densities. Methods: Thirty cadaveric specimens were analyzed in an imaging and biomechanical investigation. Computed tomography (CT) scans allowed for preoperative RSA planning and bone density analysis. Three correction methods of the glenoid were used: (1) corrective reaming with a standard baseplate, which served as the reference group (n = 10); (2) MA-RSA (n = 10); and (3) angled BIO-RSA (n = 10). Each augment group consisted of 10° (n = 5) and 20° (n = 5) corrections. Biomechanical testing included cyclic loading in an articulating setup, with optical pre- and post-cyclic micromotion measurements in a rocking horse setup. Results: There were no differences in bone density between groups based on CT scans (p > 0.126). The BIO-RSA group had higher variability in micromotion compared to the MA-RSA and reference groups (p = 0.013), and increased total micromotion compared to the reference group (p = 0.039). Both augmentations using 20° corrections had increased variance in rotational stability compared to the reference group (p = 0.043). Micromotion correlated with the subchondral bone density in the BIO-RSA group (r = −0.63, p = 0.036), but not in the MA-RSA (p > 0.178) or reference (p > 0.117) groups. Conclusions: Time-zero baseplate implant fixation is more variable with BIO-RSA and correlates with bone density. Corrections of 20° with either augmentation approach increase variability in rotational micromotion. The preoperative quantification of bone density may be useful before utilizing 20° of correction, especially when adding a bone graft in BIO-RSAs. Full article

The split-sleeve cold expansion technology is widely used in the aerospace industry, particularly for fastening holes, to enhance the fatigue life of components. However, to ensure proper assembly and improve surface integrity, reaming of the cold-expanded holes is necessary. This study investigates the effects of cold expansion and reaming processes on the fatigue performance of 7050-T7451 aluminum alloy. Fatigue tests, residual stress measurements, and microstructural analyses of the hole edges were conducted on specimens with four different hole diameters after cold expansion and reaming. It was found that the depth of reaming significantly affects fatigue life. During the cold expansion process, the compressive residual stress formed around the hole effectively improves fatigue performance. The experiments demonstrated that reaming by 0.2 mm to 0.4 mm helps eliminate minor defects, thereby improving fatigue life. However, reaming beyond 0.5 mm may lead to stress relief and the removal of dense grains at the hole edges, reducing fatigue life. Therefore, determining the optimal reaming size is crucial for enhancing the reliability of aerospace fasteners. Full article

Intramedullary nailing insertion from the proximal-to-distal femur is frequently performed for impending and complete pathological femur fractures due to osteolytic metastases. After nailing through cancer-laden bone, residual chemotherapy- and/or radiation-resistant tumor may progress. Progression of osteolysis risks future nail failure or pathological fractures. This study assesses the incidence of cancer progression following intramedullary nailing in a femur-only cohort and describes a percutaneous rod-retaining salvage technique. A single-institution, retrospective study was conducted to identify adult patients who underwent intramedullary nailing for femoral osteolytic lesions for complete or impending nail failure from 2016 to 2023. Progression was defined as enlargement of the pre-existing lesion and/or appearance of new lesions on radiographs. Surgical outcomes were assessed with a combined pain and functional score. A total of 113 patients (median age 66.8 years (IQR = 16.4); median follow-up 6.0 months (IQR = 14.5)) underwent intramedullary nailing. Sixteen patients (14.2%) exhibited post-nailing cancer progression. Pre- and postoperative radiation and chemotherapy did not decrease the odds of cancer progression. Three patients underwent initial open surgical salvage consisting of proximal femur replacement arthroplasty, and six patients did not receive salvage due to poor surgical candidacy or patient choice. Seven patients (median follow-up 10.7 months (IQR = 12.9)) received percutaneous salvage. In this group, pain and functional scores improved by 4.0 points (p = 0.0078) at two-week postoperative follow-up and 2.0 points (p = 0.0312) at the most recent follow-up (mean follow-up 13.0 ± 9.4 months). All three nonambulatory patients became ambulatory, and six patients were able to ambulate independently without walking aids. No major complications were reported 30 days postoperatively. Progression of femoral osteolytic metastases may occur following intramedullary nailing. Continued monitoring of the entire femur is needed to maintain improved functional status and to prevent catastrophic progression of pre-existing lesions or appearance of new lesions. In patients with more proximal metastases only, the customary practice of bringing a long nail from the proximal femur to distal metaphysis should be reconsidered. Furthermore, there is concern of mechanical transport of cancer cells during guide wire insertion, reaming, and rod insertion through cancer laden bone to cancer free distal bone. Full article

Background/Objectives: Tibial diaphysis fractures are common injuries resulting from high-to-low-energy traumas in patients of all age groups, but few reports currently provide complementary parameters for the assessment of bone healing processes in the postoperative period. Serum alkaline phosphatase (ALP) and the scores from the Radiographic Union Scale for Tibial Fractures (RUST) can promote new horizons in this context. Therefore, the aim of this study was to assess the behavior of ALP and RUST through within-subject comparisons from immediately post-surgery to 49 days after tibial diaphysis fracture repair. Methods: This article included four case studies where patients underwent the same procedures. Adults of both sexes aged 18 to 60 years with tibial fractures requiring surgery were included. After surgical intervention (T1), the patients were followed for 49 days after surgery, returning for follow-up appointments on the 21st (T2) and 49th (T3) days. At the follow-up appointments, new X-ray images were obtained, and blood samples were collected for ALP measurement. Results: Serum ALP levels increased by T2 following tibial reamed intramedullary nailing surgery. While this increase persisted into T3 for two patients, a decline was observed during the same period for the other two patients. Both events are indicative of the bone consolidation process, and RUST scores at the T3 corroborate this perspective for all patients included in this study. Considering that delta ALP (T3-T1 value) was lower in patients who exhibited the highest RUST score, we suggest that a synchronized analysis between ALP and RUST allows medics to diagnose bone consolidation. Conclusions: Therefore, it can be concluded that the analysis of ALP alongside RUST may be complementary for evaluating bone consolidation following tibial reamed intramedullary nailing surgery, but future studies are needed to confirm this assertion. Full article

The exploration and development of new hydrocarbon deposits is facing increasing challenges as the global shift to renewable energy sources, such as shallow geothermal deposits, wind farms, and photovoltaics, reduces the dependence on hydrocarbons. To navigate this evolving landscape, it becomes crucial to find solutions that optimize the energy extraction efficiency while maximizing the use of hydrocarbon deposits. This requires exploring opportunities in existing fields and wells, including those slated for decommissioning. This article discusses the potential for extracting resources from seemingly depleted fields, where some 60–70% of the resources remain unrecoverable due to low reservoir energy. Meeting this challenge requires the implementation of secondary and tertiary EOR methods that involve the introduction of external energy to increase reservoir pressure and enhance resource recovery. One of the proposed innovative tertiary methods involves reaming the reservoir using multiple small-diameter radial boreholes generated by a hydraulic drilling nozzle. This strategy is designed to intensify the contact between the production hole and the reservoir layer, resulting in increased or commenced production in certain cases. The described method proves to be a practical application in hydrocarbon deposits, offering the dual benefits of mitigating environmental pollution by eliminating the need for drilling new boreholes and providing a cost-effective means of accessing resources in decommissioned deposits with insufficient reservoir energy for self-exploitation. Another article points out the design variation of a hydraulic drilling nozzle tailored specifically for reaming a reservoir layer. Taking the above into account, this article provides very practical information for future projects in which paths should be sought for the design and development of hydraulic wellheads, among other things, in order to intensify the production from hydrocarbon deposits. Full article

Fastener holes are among the most common natural stress concentrators in metal structures. The life cycles of various structural elements, such as those in aircraft structures, automobiles, and rail-end bolt joints, are limited by fatigue damage around the holes. An effective approach to delay the formation and growth of fatigue macrocracks is to introduce residual hoop compressive stresses around the holes. Two methods have become established in the prestressing of fastener holes in aircraft components, split sleeve and split mandrel, which implement one-sided processes. The common disadvantage of both methods is the complex procedure due to the need for high accuracy of the initial holes. This article presents a new modified split mandrel method providing the same tightness (interference fit) with a wide tolerance of the pre-drilled hole diameters, reducing the number of technological cycle steps and production costs. To implement the new method, a functionally connected tool and a device with a hydraulic drive were developed. An extensive experimental study of 2024-T3 AA specimens was carried out to evaluate the effectiveness of the method under a high scattering of the pre-drilled holes. The new method provided a deep zone of residual hoop compressive stresses on both faces of the specimens after cold working and after hole final reaming. The removal of a plastically deformed layer around the hole of suitable thickness during the final reaming decreased the axial gradient of residual hoop stress distribution. Fatigue tests on a tensile pulsating cycle verified the effectiveness of the modified split mandrel method to significantly increase the fatigue life by 6.6 times based on ${10}^6$ cycle fatigue strength compared to the conventional case of machining the holes. The obtained S-N curves for three groups of samples with initial hole diameters of 8.0, 8.1, and 8.2 mm, which were cold worked with the same tightness of 0.32 mm and final reamed, aligned well, indicating that the new method can provide constant fatigue strength for a given stress amplitude. Full article

Background: The aim of this study was to evaluate detection rates and risk factors for unsuspected proof of bacteria, as well as clinical and radiologic outcomes following femoral shaft nonunion without clinical signs of infection treated by a single-stage surgical revision procedure including reamed intramedullary exchange nailing. Methods: A retrospective cohort study was performed in a European level I trauma center between January 2015 and December 2022. Fifty-eight patients were included who underwent reamed intramedullary exchange nailing as a single-step procedure for surgical revision of posttraumatic diaphyseal femoral nonunion without any indications of infection in medical history and without clinical signs of local infection. Clinical details of the patients were analyzed and functional and radiologic long-term outcomes were determined. Results: In all patients, with and without proof of bacteria osseous, healing could be observed. The physical component summary of the SF-12 demonstrated significantly better results at least one year after the final surgical revision in case of a negative bacterial culture during exchange nailing. Conclusions: Clinical long-term outcomes demonstrated a trend towards better results following femoral shaft nonunion revision if there was no evidence for the presence of low-grade infected nonunion. In this case, a single-stage surgical procedure may be recommended. Full article

Objective: Arthrodesis of the first metatarsophalangeal joint (MTPJ) is a reliable procedure for treating various pathologies related to this joint. However, non-union is a common and debilitating complication of the procedure. The purpose of this study was to assess whether the use of a mechanical reamer to prepare the joint surface increases the risk of non-union compared to traditional manual osteotomy. Methods: A retrospective study of all patients who underwent their first MTPJ arthrodesis surgery in our medical center between 2010 and 2015. The articular surface preparation of the MTPJ was either manually performed (Group 1) or mechanically conducted with reamers (Group 2). Data regarding demographic characteristics, indications for surgery, osteosynthesis methods, and post-operative complications, including non-union, infection, and revision rates, were collected for all patients. Results: A total of 83 patients with 92 primary first MTPJ arthrodesis (44 cases in Group 1 and 48 cases in Group 2) were included with a mean follow-up time of 45.3 months (SD 16.8). The two groups were comparable in terms of age, gender, comorbidities, indication for surgery, and follow-up time. The non-union rate was 15.9% in Group 1 and 25% in Group 2 (p = 0.28). Group 2 showed a significantly higher rate of revision surgery compared to Group 1 (43.7% vs. 22.7%, p = 0.03). Conclusions: The mechanical reamer group had a higher non-union rate and a significantly higher rate of revision surgery compared to low-energy manual joint preparation. In light of these findings, the use of mechanical reamers for joint preparation in the first MTPJ arthrodesis was discontinued at our medical center. Full article