Search Results (10)

Epiphytic lichens are integral to boreal forest ecosystems, yet their allelopathic interactions with host trees, particularly in cryolithozone regions, remain poorly understood. This study elucidates the physiological and biochemical impacts of the epiphytic lichen Physcia alnophila on Larix gmelinii (Gmelin larch), a keystone species in Siberian permafrost forests. By combining dendrochronology, GC–MS metabolomic analysis, and HPLC–ESI–MS/MS analysis, we demonstrate that the lichen’s primary metabolite, atranorin (ATR), systemically migrates from thalli into the host’s cambium, roots, and needles, with root accumulation reaching 36.3 µg g⁻¹ DW. Lichen-colonized trees exhibited severe radial growth inhibition (27%–51% reduction over five years) and suppressed apical growth, despite comparable heights to controls, indicating chronic phytotoxicity. Metabolomic profiling revealed lichen-specific polyols (e.g., arabitol, mannitol) in larch tissues, alongside elevated stress biomarkers (terpenes, sterols, phenolic acids), and significant disruptions to the tricarboxylic acid cycle and oxidative phosphorylation. These metabolic perturbations correlate with reduced monosaccharide availability and impaired energy production, directly linking ATR translocation to growth suppression. L. gmelinii exhibited compensatory responses, including increased fatty acids and arabinogalactan synthesis, suggesting adaptive mechanisms to mitigate lichen-induced stress. Our findings suggest P. alnophila as a biotic stressor that affects tree physiology in extreme climates, with implications for boreal forest resilience. This work provides an insight to the rarely pointed out species interactions, which, when combined with climate change, may alter carbon cycling and forest dynamics in permafrost ecosystems. Full article

Autophagy is a conserved mechanism for the turnover of intracellular components. Among the ‘core’ autophagy-related genes (ATGs), the cysteine protease Atg4 plays an important role in the activation of Atg8 by exposing the glycine residue at its extreme carboxyl terminus. In the insect fungal pathogen Beauveria bassiana, a yeast ortholog of Atg4 was identified and functionally analyzed. Ablation of the BbATG4 gene blocks the autophagic process during fungal growth under aerial and submerged conditions. Gene loss did not affect fungal radial growth on various nutrients, but ΔBbatg4 exhibited an impaired ability to accumulate biomass. The mutant displayed increased sensitivity to stress caused by menadione and hydrogen peroxide. ΔBbatg4 generated abnormal conidiophores with reduced production of conidia. Additionally, fungal dimorphism was significantly attenuated in gene disruption mutants. Disruption of BbATG4 resulted in significantly weakened virulence in topical and intrahemocoel injection assays. Our study indicates that BbAtg4 contributes to the lifecycle of B. bassiana via its autophagic roles. Full article

The forecasted growth in dynamic global air fleet size in the coming decades, together with the need to introduce disruptive technologies supporting net-zero emission air transport, demands more efficient design and optimization workflows. This research focuses on developing an aerodynamic optimization framework suited for multi-objective studies of small aircraft engine air-intake ducts in multiple flight conditions. In addition to the refinement of the duct’s performance criteria, the work aims to improve the economic efficiency of the process. The optimization scheme combines the advantages of Kriging-based Efficient Global Optimization (EGO) with the Radial Basis Functions (RBF)-based mesh morphing technique and the Chebyshev-type Achievement Scalarizing Function (ASF) for handling multiple objectives and design points. The proposed framework is applied to an aerodynamic optimization study of an I-31T aircraft turboprop engine intake system. The workflow successfully reduces the air-duct pressure losses and mitigates the flow distortion at the engine compressor’s front face in three considered flight phases. The results prove the framework’s potential for solving complex multi-point air-intake duct problems and the capacity of the ASF-based formulation to guide optimization toward the designer’s preferred objective targets. Full article

Chromoblastomycosis (CBM) is a disease caused by several dematiaceous fungi from different genera, and Fonsecaea is the most common which has been clinically isolated. Genetic transformation methods have recently been described; however, molecular tools for the functional study of genes have been scarcely reported for those fungi. In this work, we demonstrated that gene deletion and generation of the null mutant by homologous recombination are achievable for Fonsecaea pedrosoi by the use of two approaches: use of double-joint PCR for cassette construction, followed by delivery of the split-marker by biolistic transformation. Through in silico analyses, we identified that F. pedrosoi presents the complete enzymatic apparatus required for tryptophan (trp) biosynthesis. The gene encoding a tryptophan synthase trpB —which converts chorismate to trp—was disrupted. The ΔtrpB auxotrophic mutant can grow with external trp supply, but germination, viability of conidia, and radial growth are defective compared to the wild-type and reconstituted strains. The use of 5-FAA for selection of trp^- phenotypes and for counter-selection of strains carrying the trp gene was also demonstrated. The molecular tools for the functional study of genes, allied to the genetic information from genomic databases, significantly boost our understanding of the biology and pathogenicity of CBM causative agents. Full article

Presently, the forests of one of the most economically important tree species in Europe—Norway spruce [Picea abies (L.) Karst.]—have been disrupted and are in rapid decline due to a combination of several natural factors: extreme drought, heatwaves, and secondary damage caused by bark beetle outbreaks. The vulnerability of these forests has increased considerably over the past decade, and remote sensing methods can theoretically improve the identification of endangered forest stands. The main objective was to determine the relationship between remotely sensed characteristics of vegetation (using the normalized difference vegetation index—NDVI) and annual tree-ring growth in 180 trees through precipitation and air temperature. The research was conducted at six research plots in lowland spruce forests (319–425 m a.s.l.) in the central Czech Republic. No significant correlation between NDVI and annual ring width was observed. The primary factor limiting radial growth was lack of precipitation in the growing season; subsequently, spruce trees reacted negatively to air temperatures. A higher correlation with NDVI was observed on sites susceptible to drought, but overall, NDVI and RWI did not show similarities. This result describes that NDVI is a poor indicator for identifying low radial growth in Norway spruce stands on non-native localities in the studied area. Full article

Cutaneous melanoma emerges from the malignant transformation of melanocytes and is the most aggressive type of skin cancer. The progression can occur in different stages: radial growth phase (RGP), vertical growth phase (VGP), and metastasis. Reactive oxygen species contribute to all phases of melanomagenesis through the modulation of oncogenic signaling pathways. Tetrahydrobiopterin (BH4) is an important cofactor for NOS coupling, and an uncoupled enzyme is a source of superoxide anion (O₂^•−) rather than nitric oxide (NO), altering the redox homeostasis and contributing to melanoma progression. In the present work, we showed that the BH4 amount varies between different cell lines corresponding to distinct stages of melanoma progression; however, they all presented higher O₂^•− levels and lower NO levels compared to melanocytes. Our results showed increased NOS expression in melanoma cells, contributing to NOS uncoupling. BH4 supplementation of RGP cells, and the DAHP treatment of metastatic melanoma cells reduced cell growth. Finally, Western blot analysis indicated that both treatments act on the PI3K/AKT and MAPK pathways of these melanoma cells in different ways. Disruption of cellular redox homeostasis by the altered BH4 concentration can be explored as a therapeutic strategy according to the stage of melanoma. Full article

There are three subfamilies of patatin-related phospholipase A (pPLA) group of genes: pPLAI, pPLAII, and pPLAIII. Among the four members of pPLAIIIs (α, β, γ, δ), the overexpression of three isoforms (α, β, and δ) displayed distinct morphological growth patterns, in which the anisotropic cell expansion was disrupted. Here, the least studied pPLAIIIγ was characterized, and it was found that the overexpression of pPLAIIIγ in Arabidopsis resulted in longitudinally reduced cell expansion patterns, which are consistent with the general phenotype induced by pPLAIIIs overexpression. The microtubule-associated protein MAP18 was found to be enriched in a pPLAIIIδ overexpressing line in a previous study. This indicates that factors, such as microtubules and ethylene biosynthesis, are involved in determining the radial cell expansion patterns. Microtubules have long been recognized to possess functional key roles in the processes of plant cells, including cell division, growth, and development, whereas ethylene treatment was reported to induce the reorientation of microtubules. Thus, the possible links between the altered anisotropic cell expansion and microtubules were studied. Our analysis revealed changes in the transcriptional levels of microtubule-associated genes, as well as phospholipase D (PLD) genes, upon the overexpression of pPLAIIIγ. Overall, our results suggest that the longitudinally reduced cell expansion observed in pPLAIIIγ overexpression is driven by microtubules via transcriptional modulation of the PLD and MAP genes. The altered transcripts of the genes involved in ethylene-biosynthesis in pPLAIIIγOE further support the conclusion that the typical phenotype is derived from the link with microtubules. Full article

The growth pattern of filamentous fungi can switch between hyphal radial polar growth and non-polar yeast-like cell growth depending on the environmental conditions. Asexual conidiation after radial polar growth is called normal conidiation (NC), while yeast-like cell growth is called microcycle conidiation (MC). Previous research found that the disruption of MaH1 in Metarhizium acridum led to a conidiation shift from NC to MC. However, the regulation mechanism is not clear. Here, we found MaMsn2, an Msn2 homologous gene in M. acridum, was greatly downregulated when MaH1 was disrupted (ΔMaH1). Loss of MaMsn2 also caused a conidiation shift from NC to MC on a nutrient-rich medium. Yeast one-hybrid (Y1H) and electrophoretic mobility shift assay (EMSA) showed that MaH1 could bind to the promoter region of the MaMsn2 gene. Disrupting the interaction between MaH1 and the promoter region of MaMsn2 significantly downregulated the transcription level of MaMsn2, and the overexpression of MaMsn2 in ΔMaH1 could restore NC from MC of ΔMaH1. Our findings demonstrated that MaMsn2 played a role in maintaining the NC pattern directly under the control of MaH1, which revealed the molecular mechanisms that regulated the conidiation pattern shift in filamentous fungi for the first time. Full article

The development of natural essential oil as an alternative to synthetic chemicals in the control of postharvest decay is currently in the spotlight. In the present study, the efficacy of seven essential oils in suppressing Collelotrichum gloeosporioides identified from sweet cherry was evaluated in vitro and clove oil was proved to be the most promising inhibitor. Thus, the antifungal properties and potential mechanisms of clove oil in vitro and in vivo by fumigation and contact treatments were intensively investigated. For C. gloeosporioides, the minimal inhibitory concentrations (MIC) of clove oil in air and contact phase were 80 and 300 μL/L in vitro testing, respectively. Based on the radial growth of C. gloeosporioides mycelium in medium, the fumgitoxic ability of essential oil was observed in a dose-dependent manner, which was not as dramatic as that under in vivo conditions. Furthermore, scanning electron microscopy and transmission electron microscopy of C. gloeosporioides exposed to clove oil exhibited obviously deleterious morphological and ultrastructural alterations confirming the disruption of fungal cell wall and endomembrane system, which resulted in increasing in permeability and causing the loss of intracellular constituents. In future, essential oils, combined with nano-emulsification approaches, could be good candidates as safe and effective antifungal agents for fungal spoilage of fresh commodities. Full article

The thermophilic fungus Humicola insolens produces cellulolytic enzymes that are of great scientific and commercial interest; however, few reports have focused on its cellulase expression regulation mechanism. In this study, we constructed a creA gene (carbon catabolite repressor gene) disruption mutant strain of H. insolens that exhibited a reduced radial growth rate and stouter hyphae compared to the wild-type (WT) strain. The creA disruption mutant also expressed elevated pNPCase (cellobiohydrolase activities), pNPGase (β-glucosidase activities), and xylanase levels in non-inducing fermentation with glucose. Unlike other fungi, the H. insolens creA disruption mutant displayed lower FPase (filter paper activity), CMCase (carboxymethyl cellulose activity), pNPCase, and pNPGase activity than observed in the WT strain when fermentation was induced using Avicel, whereas its xylanase activity was higher than that of the parental strain. These results indicate that CreA acts as a crucial regulator of hyphal growth and is part of a unique cellulase expression regulation mechanism in H. insolens. These findings provide a new perspective to improve the understanding of carbon catabolite repression regulation mechanisms in cellulase expression, and enrich the knowledge of metabolism diversity and molecular regulation of carbon metabolism in thermophilic fungi. Full article