Search Results (35)

Fire is a natural disturbance in the Brazilian Cerrado that modulates the vegetation structure. Protium heptaphyllum, a woody species of the family Burseraceae, is common in this biome. The resin produced in secretory canals immersed in the phloem of the stem and leaves of this species plays important ecological and industrial roles. The aim of this study was to investigate the influence of fire on the development of resin canals in the leaves and stem of P. heptaphyllum and on the chemical profile of substances produced in the leaves. Young plants were subjected to controlled fire experiments. Leaf and stem portions were analyzed using light microscopy; the chemical compounds in the leaves were identified through gas chromatography–mass spectrometry. The percentage area occupied by secretory canals in the leaf midrib was higher in fire-treated plants than in control plants. Similarly, the density of secretory canals and their lumen area were higher in young stems (primary growth) of fire-treated plants. By contrast, although the canal density in the secondary phloem was lower in older stem portions (secondary growth) in fire-treated plants, their lumens were larger, resulting in similar data regarding the total lumen area of the secretory canals in fire-treated and control plants. The main chemical compounds identified in the leaves were vitamin E, sitosterol, α-amyrin, squalene, and β-amyrin. Three compounds showed significant increases in fire-treated plants, with vitamin E being the only one reduced by fire. Our findings reveal the plasticity of the secretory system and of the biochemical properties of the leaves of P. heptaphyllum in response to fire. These results are important when considering the current increase in fires caused by climate change and human activity in different ecosystems around the world. Full article

Water is a vital nutrient that is needed to maintain almost all biological processes in living organisms. The natural water contains two isotopes of hydrogen—protium and deuterium. Deuterium, the trace component of natural water, significantly changes its physical and chemical properties and biological action. In this review, the authors summarize data on the isotopic effects of deuterium and discuss the possible magnetic effects of isotopes and the molecular basis of the biological effects of deuterium-depleted water. The review also presents new data on the already known and potential use of deuterium-depleted water in medicine (oncology, neurology) and previously unknown new directions of its use (immunological and endocrine disorders, anemia). Based on the analysis of collected data, the authors presented mechanisms of action of deuterium-depleted water in the organism. The authors also identified the least-studied effects of deuterium-depleted water, particularly its influence on morphogenetic processes. The review discloses the perspectives on deuterium-depleted water as a tool for therapy and substantiates the risks of its uncontrolled intake. Full article

The breadth and depth of plant leaf metabolomes have been implicated in key interactions with plant enemies aboveground. In particular, divergence in plant species chemical composition—amongst neighbors, relatives, or both—is often suggested as a means of escape from insect herbivore enemies. Plants also experience strong pressure from enemies such as belowground pathogens; however, little work has been carried out to examine the evolutionary trajectories of species’ specialized chemistries in both roots and leaves. Here, we examine the GCMS detectable phytochemistry (for simplicity, hereafter referred to as specialized volatile metabolites) of the tropical tree genus Protium, testing the hypothesis that phenotypic divergence will be weaker belowground compared to aboveground due to more limited dispersal by enemies. We found that, after controlling for differences in chemical richness, roots expressed less structurally diverse compounds than leaves, despite having higher numbers of specialized volatile metabolites, and that species’ phylogenetic distance was only positively correlated with compound structural distance in roots, not leaves. Taken together, our results suggest that root specialized volatile metabolites exhibit significantly less phenotypic divergence than leaf specialized metabolites and may be under relaxed selection pressure from enemies belowground. Full article

Charge-free gaseous molecules labeled with deuterium ²H (D) atoms elute earlier than their protium-analogs ¹H (H) from most stationary GC phases. This effect is known as the chromatographic H/D isotope effect (hdIE_C) and can be calculated by dividing the retention times (t_R) of the protiated (t_R(H) ) to those of the deuterated (t_R(D)) analytes: hdIE_C = t_R(H)/t_R(D). Analytes labeled with ¹³C, ¹⁵N or ¹⁸O have almost identical retention times and lack a chromatographic isotope effect. Derivatives of cis- and trans-analytes such as cis- and trans-fatty acids also differ in their retention times. Analytes that contain trans-C=C-double bonds elute earlier in gas chromatography-mass spectrometry (GC-MS) than their cis-C=C-double bonds containing congeners. The chromatographic cis/trans-effect (ctE_C) can be calculated by dividing the retention times of the cis- by those of the trans-analytes: ctE_C = t_R(c)/t_R(t). In the present work, the hdIE_C and ctE_C values of endogenous and exogenous substances were calculated from previously reported GC-MS analyses and found to range each between 1.0009 and 1.0400. The examination suggests that the H/D-isotope effects and the cis/trans-effects observed in GC-MS are based on differences in the inter-molecular interaction strengths of the analyte derivatives with the stationary phase of GC columns. The deuterium atoms, being larger than the H atoms of the analytes, attenuate the interaction of the skeleton of the molecules with the GC stationary phase. The angulation of trans-analytes decreases the interaction of the skeleton of the molecules with the GC stationary phase, as only parts of the molecules are close enough to the GC stationary phase to interact. Other chromatographic effects caused by hydrogen (H) and fluorine (F) atoms and by stereo-isomerism are considered to be based on a similar mechanism due to the different orientation of the side chains. Full article

The scarcity of hardwoods from tropical forests makes the search for alternative species necessary for commercialization. This study aimed to establish groups of timber species from the Amazon Forest with potential for logging purposes through the assessment of their physical-mechanical properties, aiming to identify alternative species that can meet the market demands. We utilized data from the Forest Products Laboratory (LPF) (containing information on basic density and other wood mechanical properties) and the Timberflow platform, as well. We applied a multivariate cluster analysis technique with the aim of grouping species based on the technological characteristics of their wood and evaluating similarity among them to obtain homogeneous groups in terms of economic potential and utilization. The results indicated four homogeneous groups: Cluster 1 (40.72% of species, basic density-db: 690 kg m⁻³), Cluster 2 (13.92%, db: 260 and 520 kg m⁻³), Cluster 3 (27.32%, db: 550 and 830 kg m⁻³), and Cluster 4 (18.04%, db: 830 kg m⁻³). Most of the 20 listed species are classified as more commercially viable (70%), with high wood density. Species identified as alternatives include Dialium guianense and Zollernia paraensis for Dipteryx odorata, Terminalia argentea for Dinizia excelsa, Terminalia amazonia and Buchenavia grandis for Goupia glabra, and Protium altissimum and Maclura tinctoria for Hymenaea courbaril. The analysis highlighted the overexploitation of a restricted group of species and the need to find alternatives to ensure the sustainability of forest management. This study contributed to identifying species that can serve as alternatives to commercial ones, promoting a more balanced and sustainable forest management. Full article

This study aimed to assess the efficiency of the use of α-pinene and essential oils of Gaultheria procumbens, Juniperus communis, Protium heptaphyllum, and Protium pallidum in treating corn seeds (Zea mays) under storage conditions for the management of Sitophilus zeamais. Contact toxicity, fumigation, repellency, persistence, and residual effects of the targeted essential oil and phytocompound on germination were performed. G. procumbens oil, high in methyl salicylate (96%), was the most toxic in contact tests, with an LC₅₀ of 26.83 µL/20 g. P. heptaphyllum oil, containing 40.1% limonene, was the second most toxic with an LC50 of 45.78 μL/20 g. When test separately, α-pinene was more toxic than J. communis oil, which has 67% α-pinene. P. pallidum oil, with 31.17% o-cimene, also showed toxicity. In fumigation tests, the toxicity order was G. procumbens ≥ P. heptaphyllum > α-pinene > J. communis > P. pallidum. All products were repellent. G. procumbens had the longest persistence (71 days), while J. communis and α-pinene had shorter persistence. J. communis oil and α-pinene did not affect corn seed germination or vigor. The findings are crucial for managing S. zeamais in stored maize and determining the appropriate use of natural insecticides without affecting their ability to germinate and grow. Full article

Protium heptaphyllum (P. heptaphyllum), popularly known as “almacega” or “white pitch”, is widely used in folk medicine due to its antioxidant, anti-inflammatory and healing properties, attributed to its richness in flavonoids and terpenes. Therefore, this study aimed to evaluate the effects of treatment for 28 days with liposomes containing P. heptaphyllum leaf extract in obese animals. Male Wistar rats, subjected to a hypercaloric diet for 8 weeks to induce obesity (hypercaloric chow and water enriched with 30% sucrose, ad libitum), were treated with the plant formulation (1 mg kg⁻¹day⁻¹, via gavage) for 28 days. The study investigated morphological, metabolic, redox state, immunological and histological parameters in adipose and liver tissue. Rats were divided into four groups: control (C), liposomes with extract (H), obese (O) and obese treated with liposomes containing extract (OH). The results indicated that the obese group (O) presented weight gain, hepatic steatosis and alterations in metabolic and inflammatory parameters. However, treatment with liposomes (OH) reduced glucose, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), creatinine and the lipid profile. In adipose tissue, the OH group showed decreased superoxide dismutase (SOD) activity and increased glutathione S-transferase (GST) activity, in contrast to the effects observed in liver GST. In the analysis of thiobarbituric-acid-reactive substances (TBARS), it was possible to observe an increase in all groups in adipose tissue and in group O in liver tissue, in addition to a reduction in TBARS in group OH in the liver, indicating modulation of oxidative stress. The treatment also increased the concentration of IL-10 and IL-17 in the liver and decreased that of IL-6 in adipose tissue. After 28 days of treatment, these results point to the therapeutic potential of treatment with P. heptaphyllum, not necessarily only against obesity, but also an effect per se of the liposomes, possibly due to the high concentration of flavonoids present in the plant extract. Full article

Cyclophosphamide (CPA) is an alkylating agent used as a chemotherapy agent in the treatment of cancer, but it has immunosuppressive effects. Protium heptaphyllum (P. heptaphyllum) is a plant rich in triterpenes and flavonoids, with some bioactive and therapeutic properties presented in the literature. Thus, the present study aimed to investigate the chemoprotective potential of P. heptaphyllum extract inserted into liposomes against oxidative damage chemically induced by CPA. Male Swiss mice received 1.5 mg/kg of P. heptaphyllum liposomes as a pre-treatment for 14 consecutive days (via gavage) and 100 mg/kg of CPA in a single dose (via intraperitoneal) on the 15th day. After the experimental period, blood and organ samples were collected for histopathological and biochemical analyses, including superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), glutathione S-transferase (GST), reduced glutathione (GSH), thiobarbituric acid reactive substances (TBARS), ascorbic acid (ASA), carbonyl protein, cytokine measurement, and micronucleus testing. The results showed that liposomes containing P. heptaphyllum extract have an antimutagenic effect against damage induced to DNA by CPA, and that they also protect against oxidative stress, as verified by the increase in the antioxidant enzymes SOD and GPx. The improvement in alkaline phosphatase and creatinine markers suggests a beneficial effect on the liver and kidneys, respectively. However, the depletion of GSH in the liver and brain suggests the use of antioxidants for the metabolism of molecules generated in these tissues. In general, these data show good prospects for the use of P. heptaphyllum liposomes as a cancer chemoprotective agent, as well as possible antioxidant action, conceivably attributed to the flavonoids present in the plant extract. Full article

Obesity, a chronic disease, resulted from excessive consumption of high-calorie foods, leading to an energy imbalance. Protium heptaphyllum (P. heptaphyllum) was used in folk medicine for its analgesic, anti-inflammatory, and healing properties. The association of the extract from P. heptaphyllum with nanotechnology was innovative for combining high technology with active ingredients that are easily accessible in the Amazon region. This study evaluated the effect of liposomes containing the ethyl acetate fraction of the crude extract of P. heptaphyllum leaves on obesity. Male Wistar rats treated with a high-calorie diet for 8 weeks to induce obesity received treatment with the liposome formulation containing P. heptaphyllum extract (1 mg/kg/day, via gavage) for 14 days. Morphological, metabolic, redox status, immunological, and histological parameters were evaluated in the adipose and liver tissue of the animals. The groups were divided as follows: C: control; P: liposomes containing extract; O: obese, and OP: obese + liposomes containing extract. The obesity model resulted in increases in body weight, caloric intake, body fat weight, and in the lipid profile. In adipose tissue, P decreased SOD (superoxide dismutase) activity in obese animals. In the liver, a positive modulation of the extract was observed in relation to glucose, amino acids, lactate, hepatoprotective action, and anti-inflammatory activity, with a decrease in interleukin 1β (IL-1β) in obese animals. The results showed an improvement in the functional and inflammatory aspects, but the treatment was not effective in alleviating general changes related to obesity, such as weight gain, fat, glucose, triglycerides, and inflammation in adipose tissue, highlighting the complexity of responses in different organs during obesity and treatment with P. heptaphyllum. Full article

The treatment of skin and soft tissue infections (SSTIs) can be challenging due to bacterial resistance, particularly from strains like MRSA and biofilm formation. However, combining conventional antibiotics with natural products shows promise in treating SSTIs. The objective of this study is to develop a nanoemulsion-based hydrogel containing Protium spruceanum extract and mupirocin and evaluate its potential for the treatment of SSTIs. The nanoemulsion was obtained by phase inversion and subsequently characterized. The antibacterial activity was evaluated in vitro against S. aureus MRSA, including the synergism of the combination, changes in membrane permeability using flow cytometry, and the anti-biofilm effect. In addition, the irritative potential was evaluated by the HET-CAM assay. The combination exhibited synergistic antibacterial activity against S. aureus and MRSA due to the extract enhancing membrane permeability. The hydrogel demonstrated suitable physicochemical properties, inhibited biofilm formation, and exhibited low irritation. The formulation was nanometric (176.0 ± 1.656 nm) and monodisperse (polydispersity index 0.286 ± 0.011). It exhibited a controlled release profile at 48 h and high encapsulation efficacy (94.29 ± 4.54% for quercitrin and 94.20 ± 5.44% for mupirocin). Therefore, these findings suggest that the hydrogel developed could be a safe and effective option for treating SSTIs. Full article

Beekeeping directly depends on the floral biodiversity available to honey bees. In tropical regions, where nectar and pollen resources are numerous, the botanical origin of some honey is still under discussion. A precise knowledge of plants foraged by honey bees is useful to understand and certify the botanical origin of honey. In this study, attention was paid to honey samples from the French Guiana Atlantic coast where beekeepers generally place their hives in four types of biotopes: seaside vegetation, mangrove, savannah, and secondary forest. Pollen analysis of 87 honey samples enabled the identification of major plants visited by Africanized honey bees during the dry season (approximately from July to January). Through melissopalynologic analysis, 51 pollen types were identified and classified according to their relative presence. Frequently observed pollens (with relative presence > 50%) in French Guiana kinds of honey were those from Mimosa pudica, Cocos sp., Rhyncospora sp., Avicennia germinans, Paspalum sp., Spermacoce verticillata, Tapirira guianensis, Cecropia sp., Myrtaceae sp., Mauritia flexuosa sp., Solanum sp., and Protium sp. In many honeys, only M. pudica was over-represented (relative frequency > 90%). Color and electrical conductivity in French Guiana honeys exhibit significant variations, with color ranging from 27 mm to 110 mm Pfund, and electrical conductivity ranging from 0.35 to 1.22 mS/cm. Full article

Online monitoring is a key challenge for the continued development of molten salt reactor (MSR) technology. Laser-induced breakdown spectroscopy (LIBS) has previously been demonstrated to be a viable tool for monitoring aerosolized species and noble gases in real time, but the ability to discern varying isotopes in these streams has not yet been investigated for MSR applications. Tritium will form in MSRs from ternary fission and from (n,α)-reactions occurring in lithium-containing salts. This study compares three spectrometers of varying resolutions and types for measuring hydrogen isotope shifts in LIBS spectra of wetted filter paper. For each spectrometer, multivariate models were built (i.e., principal component regression, partial least squares regression, and multivariate curve resolution) to quantify the isotope ratio. The top models were then modified and corrected to apply the models to aerosol samples with varying isotope ratios. This novel calibration strategy offers an 82% reduction in volume of the calibration samples needed and is a more viable pathway for calibrating deployable LIBS systems. Lastly, this calibration model was compared with an all-aerosol trained model for monitoring hydrogen isotopes during a real-time test where the protium/deuterium ratio, along with representative salt species (i.e., lithium, sodium, and potassium) were adjusted dynamically. Results of this test validated the predictive capabilities of the transferred model and highlighted the capabilities of LIBS for real-time monitoring of MSR effluent streams. Full article

Experimental findings to contribute to the preliminary design of a metal foil pump for fuel separation in the Direct Internal Recycling loop of the DEMO fusion device are presented. In parametric studies on a small-scale superpermeation experiment with a microwave plasma source and two different metal foil materials, niobium Nb and vanadium V, a substantial increase in permeation with plasma power and with a decrease in pressure was observed. To ease operation in the typical fusion environment, in-situ heating procedures were developed to recover from impurity contamination. The temperature independence of plasma-driven permeation from 600 to 900 °C metal foil temperature was demonstrated. No proof of an isotopic effect for plasma-driven permeation of protium and deuterium could be found. The highest repeatable permeation flux achieved was 6.7 Pa∙m³/(m²∙s) or ~5.5 × 10⁻³ mol H/(m²∙s). The found compression ratios do safely allow the operation of the metal foil pump using ejector pumps as backing stages for the permeate. In a dedicated experimental setup, the operation of the plasma source in a strong magnetic field was tested. Parametric studies of pressure, power input, magnetic flux density, field gradient and field angle are presented. Full article

The influence of a single ²H/¹H replacement on the frequency generation of different-size bubbles in the human interferon alpha-17 gene (IFNA17) under various energies was studied by a developed algorithm and mathematical modeling without simplifications or averaging. This new approach showed the efficacy of researching DNA bubbles and open states both when all hydrogen bonds in nitrogenous base pairs are protium and after an ²H-substitution. After a single deuterium substitution under specific energies, it was demonstrated that the non-coding region of IFNA17 had a more significant regulatory role in bubble generation in the whole gene than the promoter had. It was revealed that a single deuterium substitution for protium has an influence on the frequency generation of DNA bubbles, which also depends on their size and is always higher for the smaller bubbles under the largest number of the studied energies. Wherein, compared to the natural condition under the same critical value of energy, the bigger raises of the bubble frequency occurrence (maximums) were found for 11–30 base pair (bp) bubbles (higher by 319%), 2–4 bp bubbles (higher by 300%), and 31 bp and over ones (higher by 220%); whereas the most significant reductions of the indicators (minimums) were observed for 11–30 bp bubbles (lower by 43%) and bubbles size over 30 bp (lower by 82%). In this study, we also analyzed the impact of several circumstances on the AT/GC ratio in the formation of DNA bubbles, both under natural conditions and after a single hydrogen isotope exchange. Moreover, based on the obtained data, substantial positive and inverse correlations were revealed between the AT/GC ratio and some factors (energy values, size of DNA bubbles). So, this modeling and variant of the modified algorithm, adapted for researching DNA bubbles, can be useful to study the regulation of replication and transcription in the genes under different isotopic substitutions in the nucleobases. Full article