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24 pages, 631 KB  
Article
ContractNerd: An AI Tool to Find Unenforceable, Ambiguous, and Prejudicial Clauses in Contracts
by Musonda Sinkala, Yuge Duan, Haowen Yuan and Dennis Shasha
Electronics 2025, 14(21), 4212; https://doi.org/10.3390/electronics14214212 - 28 Oct 2025
Viewed by 2157
Abstract
Contractual agreements often contain clauses that are unfair, creating unjust suffering in one party to the agreement. ContractNerd leverages advanced Large Language Models (LLMs) to analyze contractual agreements and identify issues across four categories: missing clauses, unenforceable clauses, legally sound clauses, and legal [...] Read more.
Contractual agreements often contain clauses that are unfair, creating unjust suffering in one party to the agreement. ContractNerd leverages advanced Large Language Models (LLMs) to analyze contractual agreements and identify issues across four categories: missing clauses, unenforceable clauses, legally sound clauses, and legal but risky clauses. By using a structured methodology that integrates LLM-based clause comparison, enforceability checks against jurisdiction-specific regulations, and assessments of risk-inducing traits, ContractNerd provides a comprehensive analysis of contractual terms. To evaluate the tool’s effectiveness, we compare its analyses with those from existing platforms on rental clauses that have led to court litigation. ContractNerd’s interface helps users (both drafters and signing parties) to navigate complex contracts, offering actionable insights to flag legal risks and disputes. Full article
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16 pages, 602 KB  
Review
The Need for Social Developmental Research on Internal and External Motivation to Respond Without Prejudice
by Katelyn J. Pitcher and Rhiannon L. Smith
Youth 2025, 5(4), 103; https://doi.org/10.3390/youth5040103 - 29 Sep 2025
Viewed by 925
Abstract
Ethnic and racial bias peaks during middle childhood. The development of internal motivation to respond without prejudice (IMRWP, i.e., a desire to act according to internalized beliefs about equality) and external motivation to respond without prejudice (EMRWP, i.e., the desire to appear non-prejudicial [...] Read more.
Ethnic and racial bias peaks during middle childhood. The development of internal motivation to respond without prejudice (IMRWP, i.e., a desire to act according to internalized beliefs about equality) and external motivation to respond without prejudice (EMRWP, i.e., the desire to appear non-prejudicial in order to avoid social censure or rejection) may help to reduce children’s racial bias. However, IMRWP and EMRWP have almost exclusively been studied among adults. The current review seeks to situate IMRWP and EMRWP research within established developmental psychological theories in order to provide a framework for needed research in this area. We describe how peer norms may contribute to or hinder internal and external MRWP development over the course of middle childhood. We consider same- and cross-ethnicity/race friendships as possible sources of IMRWP development. Finally, we address the potential for schools to contribute to both IMRWP and EMRWP development through policies that emphasize intergroup contact and education about race and racism. Given the urgent threat that ethnic and racial prejudice poses to society, better understanding the development of motivations to respond without prejudice from early in life is critical. Full article
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17 pages, 238 KB  
Article
Leveraging ChatGPT in K-12 School Discipline: Potential Applications and Ethical Considerations
by Joseph C. Kush
AI 2025, 6(7), 139; https://doi.org/10.3390/ai6070139 - 27 Jun 2025
Viewed by 3396
Abstract
This paper investigates the utility of an Artificial Intelligence (AI) system, as it examines AI-generated output when prompted with a series of vignettes reflecting typical disciplinary challenges encountered by K-12 students. Specifically, the study focuses on possible racial biases embedded within ChatGPT, a [...] Read more.
This paper investigates the utility of an Artificial Intelligence (AI) system, as it examines AI-generated output when prompted with a series of vignettes reflecting typical disciplinary challenges encountered by K-12 students. Specifically, the study focuses on possible racial biases embedded within ChatGPT, a prominent language-based AI system. An analysis of AI-generated responses to disciplinary vignettes involving students of diverse racial backgrounds uncovered subtle yet prevalent racial biases present in the output. The findings indicate that while ChatGPT generally offered recommendations that were consistent and appropriate across racial lines, instances of pronounced and prejudicial disparities were observed. This study highlights the critical necessity of acknowledging and rectifying racial biases inherent in AI systems, especially in contexts where such technologies are utilized for school discipline. It provides guidance for educators and practitioners on the cautious use of AI-driven tools in disciplinary contexts, and emphasizes the ongoing imperative to mitigate biases in AI systems to ensure fair and equitable outcomes for all students, irrespective of race or ethnicity. Full article
(This article belongs to the Special Issue AI Bias in the Media and Beyond)
12 pages, 704 KB  
Article
A Path Out: Using Video Games to Reduce Prejudice Towards Refugees
by Liam Cross, Gray Atherton and Chris Stiff
Behav. Sci. 2025, 15(5), 583; https://doi.org/10.3390/bs15050583 - 26 Apr 2025
Cited by 3 | Viewed by 1343
Abstract
Historically, research on video games has centred on their potentially adverse effects, though more recently, work has started to explore the benefits. Here, we investigate whether playing a video game portraying a refugee’s plight in escaping war-torn Syria could affect implicit and explicit [...] Read more.
Historically, research on video games has centred on their potentially adverse effects, though more recently, work has started to explore the benefits. Here, we investigate whether playing a video game portraying a refugee’s plight in escaping war-torn Syria could affect implicit and explicit attitudes towards that social group. We show that after 30 min of game play embodying a Syrian refugee, participants showed reduced prejudicial attitudes and increased empathy towards Syrian refugees compared to those playing a mechanically similar but contextually unrelated game. While implicit attitudes followed the same direction, this difference was not statistically significant. Potential theoretical underpinnings of these findings, including perspective taking, embodiment, and contact perspectives for prejudice reduction, are discussed. Full article
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14 pages, 1922 KB  
Article
Inoculation with Bradyrhizobium elkanii Reduces Nitrogen Fertilization Requirements for Pseudalbizzia niopoides, a Multipurpose Neotropical Legume Tree
by Rafael Barroca Silva, Cristiane de Pieri, Leonardo José Silva da Costa, Mellina Nicácio da Luz, Antonio Ganga, Gian Franco Capra, José Raimundo de Souza Passos, Magali Ribeiro da Silva and Iraê Amaral Guerrini
Nitrogen 2025, 6(2), 26; https://doi.org/10.3390/nitrogen6020026 - 12 Apr 2025
Cited by 1 | Viewed by 2045
Abstract
This study investigated the effects of Bradyrhizobium elkanii inoculation and nitrogen (N) fertilization on the growth of Pseudalbizzia niopoides seedlings in a nursery and their subsequent performance in soil. P. niopoides is a legume tree native to Latin American tropical forests, known to [...] Read more.
This study investigated the effects of Bradyrhizobium elkanii inoculation and nitrogen (N) fertilization on the growth of Pseudalbizzia niopoides seedlings in a nursery and their subsequent performance in soil. P. niopoides is a legume tree native to Latin American tropical forests, known to nodulate but with no previously identified rhizobial partner. Seedlings were grown in a nursery under varying N fertilization rates (0, 250, 500, 1000, and 2000 mg L−1) with and without B. elkanii inoculation. Morphological traits, nodulation, and post-planting growth were assessed. Both inoculation and N fertilization significantly enhanced seedling growth in the nursery. However, high N rates suppressed nodulation and caused root toxicity. Inoculated seedlings exhibited improved growth after planting, particularly at lower N rates. Notably, inoculated seedlings without added N demonstrated vigorous new root proliferation after three months, highlighting the beneficial effects of the symbiosis. In terms of nitrogen fertilization in nurseries, a N rate up to 500 mg L−1 produced satisfactory plant growth and no prejudicial effects on the symbiosis establishment. However, it is possible to raise seedlings even in the 0 mg L−1 N rate, with a vigorous root emission during the post-planting growth. This study provides valuable insights into the interaction between a specific rhizobia strain and P. niopoides, with implications for nursery practices and sustainable agroforestry systems. Full article
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19 pages, 582 KB  
Article
Can Jurors Disregard Inadmissible Evidence? Using the Multiphase Optimization Strategy to Test Interventions Derived from Cognitive and Social Psychological Theories
by Pamela N. Sandberg, Tess M. S. Neal and Karey L. O’Hara
Behav. Sci. 2025, 15(1), 7; https://doi.org/10.3390/bs15010007 - 26 Dec 2024
Cited by 2 | Viewed by 2608
Abstract
Inadmissible evidence generally biases jurors toward guilty verdicts; jurors who hear inadmissible evidence are more likely to convict than jurors not exposed to inadmissible evidence—even when admissible evidence is constant. When inadmissible evidence is introduced, the common legal remedy is judicial instructions to [...] Read more.
Inadmissible evidence generally biases jurors toward guilty verdicts; jurors who hear inadmissible evidence are more likely to convict than jurors not exposed to inadmissible evidence—even when admissible evidence is constant. When inadmissible evidence is introduced, the common legal remedy is judicial instructions to jurors to disregard it. Appeals courts repeatedly affirm instructions to disregard as a sufficient safeguard of defendants’ constitutional rights, despite research finding that jurors do not disregard when instructed. The goals of this research were to (1) test the main and interactive effects of four theory-driven candidate strategies to help jurors disregard inadmissible evidence (i.e., inducing suspicion, giving a substantive reason for disregarding, committing to disregarding, advising future jurors) and identify an optimized intervention package, and (2) evaluate whether adding the optimized intervention package showed more favorable effects than judicial instructions only. Study 1 used a 24 full factorial randomized controlled trial to evaluate the four candidate intervention strategies. A synergistic interaction among the candidate components suggested an optimized intervention package comprising all four interventions. Study 2 used a parallel four-arm randomized controlled trial to compare conviction rates in the same hypothetical murder trial under four conditions: (1) no exposure to inadmissible evidence, (2) exposure to inadmissible evidence without objection, (3) exposure to inadmissible evidence + judicial instructions (“standard practice”), and (4) exposure + judicial instructions + optimized intervention package. Across both studies, mock jurors who received the optimized intervention package returned significantly lower conviction rates than comparison conditions. These findings show early promise that novel intervention strategies may assist jurors in disregarding inadmissible evidence. Interpretation, limitations, and calls to action are discussed. Full article
(This article belongs to the Special Issue Social Cognitive Processes in Legal Decision Making)
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26 pages, 18749 KB  
Article
Unveiling Hidden Histories: Disability in Ancient Egypt and Its Impact on Today’s Society—How Can Disability Representation in Museums Challenge Societal Prejudice?
by Nevine Nizar Zakaria
Soc. Sci. 2024, 13(12), 647; https://doi.org/10.3390/socsci13120647 - 29 Nov 2024
Cited by 2 | Viewed by 9023
Abstract
The representation of disabled individuals in museum spaces worldwide has sparked substantial debate in recent decades. It has become increasingly evident that disabled people’s lives and experiences have been overlooked, under/misrepresented in museum narratives, or as museum professionals and academics have highlighted, ‘buried [...] Read more.
The representation of disabled individuals in museum spaces worldwide has sparked substantial debate in recent decades. It has become increasingly evident that disabled people’s lives and experiences have been overlooked, under/misrepresented in museum narratives, or as museum professionals and academics have highlighted, ‘buried in the footnotes’ of history. Museums can either challenge or continue such exclusion through their actions. This marginalization of disabled people from our present museums narratives contributes to the perpetuation of prejudice and systematic biases that reinforce their exclusion in contemporary society. Nevertheless, the call for change has never been more pressing. Museums, as ‘agents of social change’, are required to not only address the absence of disabled people in their narratives but to combat prejudicial ideas about disability and drive societal changes to support social inclusion. This paper intends to use the recent movement of disability representation to envisage how national museums in Egypt can challenge societal prejudice by representing disability narratives from ancient Egypt in their exhibitions. The historical perspective of disability in ancient Egypt is a compelling example for illustrating social acceptance and the level of inclusion for disabled individuals in contrast to the negative stereotypes prevalent in contemporary Egyptian society. By adopting the new emergent approach of ‘Representing Disability’. Egyptian museums can stimulate discussions on disability issues and advocate for social change while connecting disabled individuals with a history that acknowledges their contributions and existence. The paper employed qualitative research methods to answer the following research question: what impact do representations of disability from ancient Egypt have on the way national museums engage with societal discourses about disability? Please note the article includes images of human remains. Full article
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9 pages, 2757 KB  
Article
Resection of Calcified and Giant Thoracic Disc Herniation Through Bilateral Postero-Lateral Approach and 360° Cord Release: A Technical Note
by Ismail Zaed, Benjamin Pommier, Gabriele Capo and Cédric Y. Barrey
J. Clin. Med. 2024, 13(22), 6807; https://doi.org/10.3390/jcm13226807 - 12 Nov 2024
Viewed by 2558
Abstract
Background/Objectives: Surgical treatment of thoracic disc herniation (TDH) is risky and technically demanding due to its proximity to the spinal cord and the high possibility of the TDH being calcified (up to 40%), making the resection even more complex. Calcified TDH may be [...] Read more.
Background/Objectives: Surgical treatment of thoracic disc herniation (TDH) is risky and technically demanding due to its proximity to the spinal cord and the high possibility of the TDH being calcified (up to 40%), making the resection even more complex. Calcified TDH may be resected from an anterior via thoracotomy/thoracoscopy, lateral extra-cavitary, or a postero-lateral approach. Here, we present our experience in managing such pathology with an original technique Methods: This original technique, used successfully in more than 40 patients, is introduced, with a precise description of the surgical anatomy and the surgical steps to take. Indications for surgical management and neurological outcomes are also analyzed. This surgical approach consisted of transverso-pediculectomy, most often bilaterally, partial vertebral body drilling, 360° release of the cord, and short fixation. Results: A total of 44 patients were collected, with a mean age of 52.4 ± 11.7 years. Seven patients (15.9%) had complete calcifications, and thirty-one had partial calcifications (70.5%), while the remaining six did not have signs of calcifications. There were only 4 intraoperative complications (2 dural tears and 2 loss of evoked potentials). The TDH could be resected in total for 39 patients (88.6%) and partially, according to the “floating” technique, in 5 patients (11.4%). In the postoperative follow-up, all of the patients except two (presenting with sensory aggravation) reported an improvement in neurological conditions leading to an overall risk of neurological aggravation of 4.5%. Conclusions: The bilateral postero-lateral approach provides a large decompression of the cord (360°) and gives safe access to the TDH, even calcified, permitting high rates of total resection. It also prevents any prejudicial pressure on the spinal cord, reducing the risk of severe postoperative deficits and permitting optimal instrumentation (pedicle screw-based) of the spinal segment. The surgical sequence to resect the bony structures around the spinal cord is of great importance. Full article
(This article belongs to the Special Issue Spine Surgery and Rehabilitation: Current Advances and Future Options)
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21 pages, 301 KB  
Article
Leibniz and the Religion of the Mohammadans
by Christopher Johns
Religions 2024, 15(9), 1087; https://doi.org/10.3390/rel15091087 - 6 Sep 2024
Cited by 1 | Viewed by 4263
Abstract
Throughout his correspondence and writings, Leibniz made a number of passing references to the religion of the Mohammadans (Islam) and to several Islamic commentators. Recent literature on these references has placed them in the context of Leibniz’s political and historical interests that largely [...] Read more.
Throughout his correspondence and writings, Leibniz made a number of passing references to the religion of the Mohammadans (Islam) and to several Islamic commentators. Recent literature on these references has placed them in the context of Leibniz’s political and historical interests that largely reflect his Eurocentric prejudices. The purpose of this paper is to extract a more detailed and systematic view of Leibniz’s knowledge of and interest in the religion, through Leibniz’s remarks on Islam in relation to Christian doctrines such as the Trinity and the Incarnation, through the commentary of Maimonides and the Christian Averroists, and through a (partly speculative) comparison of three types of theodicy. The paper concludes that while Leibniz knew very little about Islam’s actual doctrines, and that he was subject to the prejudicial views of his time, he understood Islam, as he did Christianity, as largely in conformity with natural (rational) religion. At the same time, his interest in its specific doctrines was primarily instrumental, that is, as correctives to certain abusive practices and misunderstandings persisting within Christianity, which could then explain for him why Islam prevailed in the East. Full article
21 pages, 342 KB  
Article
Structural Anti-Roma Racism in Italian Middle Schools
by Concetta Smedile and Antonia Ramírez-García
Societies 2024, 14(8), 153; https://doi.org/10.3390/soc14080153 - 13 Aug 2024
Cited by 2 | Viewed by 2680
Abstract
This study examines persistent racism, despite its formal denial, through an interdisciplinary approach that combines critical race theories, whiteness study and social psychology. It questions whether the analytical and empirical distinction between different forms of prejudice—despite their coexistence—can yield positive outcomes in the [...] Read more.
This study examines persistent racism, despite its formal denial, through an interdisciplinary approach that combines critical race theories, whiteness study and social psychology. It questions whether the analytical and empirical distinction between different forms of prejudice—despite their coexistence—can yield positive outcomes in the fight against racism. Considering the case of the Roma community in Italy, specifically within the universalistic approach of the Italian school system, the level of prejudice was measured in a sample of 305 middle school teachers. The main findings confirm the persistence of blatant prejudice concealed beneath a veneer of subtle racism and reveal that an educational institution’s rhetorical commitment to democracy may not substantially impact the behavior of democratic teachers when interacting with Roma individuals. The systemic inequalities ingrained within the education system are reminiscent of colonial times, where practices that once suppressed the potential of Africans are now used against Italian Roma citizens. This article finally suggests how teacher training might be improved to reduce racism, based on the different profiles of prejudicial attitudes detected among teachers. Full article
15 pages, 1697 KB  
Article
Eight Weeks of Intermittent Exercise in Hypoxia, with or without a Low-Carbohydrate Diet, Improves Bone Mass and Functional and Physiological Capacity in Older Adults with Type 2 Diabetes
by Raquel Kindlovits, Ana Catarina Sousa, João Luís Viana, Jaime Milheiro, Bruno M. P. M. Oliveira, Franklim Marques, Alejandro Santos and Vitor Hugo Teixeira
Nutrients 2024, 16(11), 1624; https://doi.org/10.3390/nu16111624 - 26 May 2024
Cited by 11 | Viewed by 5229 | Correction
Abstract
In an increasingly aging and overweight population, osteoporosis and type 2 diabetes (T2DM) are major public health concerns. T2DM patients experience prejudicial effects on their bone health, affecting their physical capacity. Exercise in hypoxia (EH) and a low-carbohydrate diet (LCD) have been suggested [...] Read more.
In an increasingly aging and overweight population, osteoporosis and type 2 diabetes (T2DM) are major public health concerns. T2DM patients experience prejudicial effects on their bone health, affecting their physical capacity. Exercise in hypoxia (EH) and a low-carbohydrate diet (LCD) have been suggested for therapeutic benefits in T2DM, improving bone mineral content (BMC) and glycemic control. This study investigated the effects of EH combined with an LCD on body composition and functional and physiologic capacity in T2DM patients. Older T2DM patients (n = 42) were randomly assigned to the following groups: (1) control group: control diet + exercise in normoxia; (2) EH group: control diet + EH; (3) intervention group: LCD + EH. Cardiopulmonary tests (BRUCE protocol), body composition (DEXA), and functional capacity (6MWT, handgrip strength) were evaluated. Body mass index (kg/m2) and body fat (%) decreased in all groups (p < 0.001). BMC (kg) increased in all groups (p < 0.001) and was significantly higher in the EH and EH + LCD groups (p < 0.001). VO2peak improved in all groups (p < 0.001), but more so in the hypoxia groups (p = 0.019). Functional capacity was increased in all groups (p < 0.001), but more so in the EH group in 6MWT (p = 0.030). EH with and without an LCD is a therapeutic strategy for improving bone mass in T2DM, which is associated with cardiorespiratory and functional improvements. Full article
(This article belongs to the Special Issue Diet Quality, Cardiometabolic Risk and Diabetes)
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10 pages, 1551 KB  
Article
Antibacterial Effect of Ozone on Cariogenic Bacteria and Its Potential Prejudicial Effect on Dentin Bond Strength—An In Vitro Study
by Marta Santos, Flávia Leandro, Helena Barroso, António H. S. Delgado, Luís Proença, Mário Polido and Joana Vasconcelos e Cruz
Pharmaceutics 2024, 16(5), 614; https://doi.org/10.3390/pharmaceutics16050614 - 2 May 2024
Cited by 9 | Viewed by 2440
Abstract
Ozone is increasingly utilized in dental caries treatment due to its antibacterial properties. In a context of limited studies and no consensus on protocols, this research aims to assess ozone’s antibacterial efficacy on cariogenic bacteria and its potential adverse impact on dentin bond [...] Read more.
Ozone is increasingly utilized in dental caries treatment due to its antibacterial properties. In a context of limited studies and no consensus on protocols, this research aims to assess ozone’s antibacterial efficacy on cariogenic bacteria and its potential adverse impact on dentin bond strength. Streptococcus mutans, Streptococcus sobrinus, Lactobacillus casei, and Actinomyces naeslundii suspensions were exposed to 40 μg/mL of ozone gas and 60 μg/mL of ozonated water (80 s) via a medical ozone generator. Negative and positive control groups (chlorhexidine 2%) were included, and UFC/mL counts were recorded. To examine microtensile bond strength (µTBS), 20 human molars were divided into four groups, and class I cavities were created. After ozone application, samples were restored using an etch-and-rinse and resin composite, then sectioned for testing. The SPSS v. 28 program was used with a significance level of 5%. The µTBS results were evaluated using one-way ANOVA, Tukey HSD, and Games-Howell. Bacterial counts reduced from 106 to 101, but dentin µTBS was significantly impacted by ozone (ANOVA, p < 0.001). Despite ozone’s attractive antibacterial activity, this study emphasizes its detrimental effect on dentin adhesion, cautioning against its use before restorative treatments. Full article
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16 pages, 1837 KB  
Hypothesis
Technical Requirements for 2023 IMO GHG Strategy
by Chunchang Zhang, Jia Zhu, Huiru Guo, Shuye Xue, Xian Wang, Zhihuan Wang, Taishan Chen, Liu Yang, Xiangming Zeng and Penghao Su
Sustainability 2024, 16(7), 2766; https://doi.org/10.3390/su16072766 - 27 Mar 2024
Cited by 24 | Viewed by 9512
Abstract
The 80th session of the IMO Maritime Environment Protection Committee (MEPC 80) adopted the 2023 IMO Strategy on the Reduction of GHG Emissions from Ships (2023 IMO GHG Strategy), with enhanced targets to tackle harmful emissions. This study strives to provide an exact [...] Read more.
The 80th session of the IMO Maritime Environment Protection Committee (MEPC 80) adopted the 2023 IMO Strategy on the Reduction of GHG Emissions from Ships (2023 IMO GHG Strategy), with enhanced targets to tackle harmful emissions. This study strives to provide an exact interpretation of the target of the 2023 IMO GHG Strategy and reveal the technical requirements therein. Decarbonization targets were expressed in IMO GHG emission scenarios for specifications. Model calculations and parameterizations were in line with IMO GHG reduction principles and decarbonizing practices in the shipping sector to avoid the prejudicial tendency of alternative fuels and the overestimated integral efficiency of short-term measures in existing predictions. IMO DCS data were used for the first time to gain reliable practical efficiencies of newly adopted regulations and further reduce the model uncertainty. The results demonstrated that the decarbonization goals for emission intensity were actually 51.5–62.5% in the IMO GHG reduction scenarios, which was much higher than the IMO recommended value of 40% as the target. Combined with the continuous applications of short-term measures, onshore power and regulations were required to contribute their maximum potential no later than the year 2030. Even so, considerable penetration (15.0–26.0%) of alternative fuels will be required by 2030 to achieve the decarbonization goals in the 90% and 130% scenarios, respectively, both far beyond the expected value in the 2023 IMO GHG Strategy (i.e., 5–10%). Until 2050, decarbonization from alternative fuels is required to achieve ~95%. Sustainable biodiesel and LNG are the necessary choices in all time periods, while the roles of e-methanol and e-ammonia deserve to be considered in the long term. Our findings highlight the intense technical requirements behind the 2023 IMO GHG Strategy and provide a pathway option for a fair and impartial transition to zero GHG emissions in the shipping sector, which might be meaningful to policymakers. Full article
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34 pages, 3400 KB  
Article
Exploring Omega-3′s Impact on the Expression of Bone-Related Genes in Meagre (Argyrosomus regius)
by Leticia Luján-Amoraga, Belén Delgado-Martín, Cátia Lourenço-Marques, Paulo J. Gavaia, Jimena Bravo, Narcisa M. Bandarra, David Dominguez, Marisol S. Izquierdo, Pedro Pousão-Ferreira and Laura Ribeiro
Biomolecules 2024, 14(1), 56; https://doi.org/10.3390/biom14010056 - 31 Dec 2023
Cited by 3 | Viewed by 2968
Abstract
Dietary supplementation with Omega-3 fatty acids seems to promote skeletal health. Therefore, their consumption at imbalanced or excessive levels has offered less beneficial or even prejudicial effects. Fish produced in aquaculture regimes are prone to develop abnormal skeletons. Although larval cultures are usually [...] Read more.
Dietary supplementation with Omega-3 fatty acids seems to promote skeletal health. Therefore, their consumption at imbalanced or excessive levels has offered less beneficial or even prejudicial effects. Fish produced in aquaculture regimes are prone to develop abnormal skeletons. Although larval cultures are usually fed with diets supplemented with Omega-3 Long Chain Polyunsaturated fatty acids (LC-PUFAs), the lack of knowledge about the optimal requirements for fatty acids or about their impact on mechanisms that regulate skeletal development has impeded the design of diets that could improve bone formation during larval stages when the majority of skeletal anomalies appear. In this study, Argyrosomus regius larvae were fed different levels of Omega-3s (2.6% and 3.6% DW on diet) compared to a commercial diet. At 28 days after hatching (DAH), their transcriptomes were analyzed to study the modulation exerted in gene expression dynamics during larval development and identify impacted genes that can contribute to skeletal formation. Mainly, both levels of supplementation modulated bone-cell proliferation, the synthesis of bone components such as the extracellular matrix, and molecules involved in the interaction and signaling between bone components or in important cellular processes. The 2.6% level impacted several genes related to cartilage development, denoting a special impact on endochondral ossification, delaying this process. However, the 3.6% level seemed to accelerate this process by enhancing skeletal development. These results offered important insights into the impact of dietary Omega-3 LC-PUFAs on genes involved in the main molecular mechanism and cellular processes involved in skeletal development. Full article
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13 pages, 5474 KB  
Case Report
Type I Dentin Dysplasia: The Literature Review and Case Report of a Family Affected by Misrecognition and Late Diagnosis
by Alessandra Putrino, Martina Caputo, Angela Galeotti, Enrico Marinelli and Simona Zaami
Medicina 2023, 59(8), 1477; https://doi.org/10.3390/medicina59081477 - 17 Aug 2023
Cited by 13 | Viewed by 7239
Abstract
Background and Objectives: Type I dentin dysplasia (DD-I) is a rare genetic disorder with autosomal dominant or recessive inheritance at risk of late or long-misunderstood diagnosis because the teeth, compared to other degenerative dentin diseases, do not have coronal defects and/or alterations [...] Read more.
Background and Objectives: Type I dentin dysplasia (DD-I) is a rare genetic disorder with autosomal dominant or recessive inheritance at risk of late or long-misunderstood diagnosis because the teeth, compared to other degenerative dentin diseases, do not have coronal defects and/or alterations but only at the root level (absent, conical, pointed roots, and obliterated pulp canals). The first radiographic suspicion often occurs only in case of sudden mobility and/or abscesses of the permanent teeth. Genetic tests confirm the diagnosis. Case Presentation: This case report describes the oral and radiographic characteristics of two siblings, 12 and 10 years old, a male and a female, at an early age affected by DD-I, whose diagnosis was made for a first orthodontic visit. The father and the older child had already undergone dental and orthodontic treatments, respectively, without the disease being suspected by the dentist. Results: Genetic tests support the diagnosis of DD-I. Following the diagnosis, the patients began a process of close periodic checks every 3–4 months to monitor their situation. The male child lost upper lateral incisors, which were then replaced with a light nylon removable prosthesis. Conclusions: The ability to recognize the radiographic features characteristic of DD-I is very important to avoid prejudicial diagnostic delays and to be able to plan the long-term treatment of these patients better, especially when the pathology was primarily misrecognized in the family. Full article
(This article belongs to the Special Issue Medicine and Dentistry: New Methods and Clinical Approaches)
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