Search Results (3,610)

Chinese parents have a tendency to compare their children with peers. However, little is known about the implications of parental social comparisons for parent–child interactions. The current study investigated the associations between parental social comparisons and parent–child closeness among 317 Chinese parents of 5- to 6-year-old children. In addition, the mediating role of mindful parenting and the moderating role of parental education anxiety were examined. Results showed that parents’ upward contrast was negatively associated with parent–child closeness, upward identification was positively associated with parent–child closeness, whereas downward contrast and downward identification were unrelated to parent–child closeness. Additionally, mindful parenting mediated the relations of parental upward contrast and upward identification to parent–child closeness. Moreover, parents’ education anxiety moderated the negative association between upward contrast and mindful parenting, such that this association was stronger in parents with lower education anxiety levels. These findings highlight the importance of guiding parents to avoid non-constructive social comparison strategies and helping them manage their education anxiety, with potential implications for promoting positive parent–child interactions. Full article

The reaction of either the L or D enantiomer of H₂N-C*H(R)CO₂⁻ (R = -CH₂SH cysteine, C; -C(SH)(CH₃)₂, penicillamine, PN; or -CH₂CH₂SH, homocysteine, HC) with an imidazole-4-carboxaldehyde and nickel(II) acetate in methanol yields a single stereoisomer of a thiazolidine (from C or PN) or a thiazine (from HC) nickel complex. Five pairs of enantiomeric products were prepared and characterized by IR, ESI MS, EA, and single crystal structure determination. There is retention of chirality for the thiazolidine and thiazine complexes on ring position 4, C_α of the parent amino acid, and transfer of chirality to the newly generated stereogenic centers, ring positions 3 (the amino acid nitrogen atom, N_AA) and 2 (the aldehyde carbon atom, C_ald). For the thiazolidines, the new stereogenic centers, N_AA, and C_ald, have identical stereochemical assignments to one another and to the assignment of the alpha carbon atom, either all R from the L enantiomers of C and PN or all S from the D enantiomers of C and PN. For the thiazine products from HC, the newly generated stereogenic centers, ring positions 3 (N_AA) and 2 (C_ald), are identical to one another but opposite to that of the retained stereogenic center (ring position 4, the alpha carbon atom). Regardless of stereochemical assignment (R or S), the hydrogen atoms of C_α, N_AA, and C_ald, ring positions 4, 3, and 2, are always all cis to one another for the five pairs of enantiomers examined. This is a consequence of the fact that the thiazolidine and thiazine rings are fused to two other chelate rings of the complexes, which seems to explain the high stereospecificity observed in these systems. Full article

Opisthorchis viverrini (OV) is a liver fluke endemic to the Lower Mekong Basin. Infections often begin in childhood and are causally linked to cholangiocarcinoma, an often-fatal bile duct cancer. Anthelmintic treatment is the primary control strategy, but infection can recur. Therefore, additional strategies are needed. This study assessed the impact of “The Magic Glasses Opisthorchiasis” (MGO), a cartoon-based intervention, on schoolchildren’s OV-related knowledge, attitudes and practices (KAP). A cluster (school)-randomised controlled trial was conducted in Cambodia, Laos and Thailand. Clusters were randomised into either school health education only or with MGO. OV KAP was measured using a standardised questionnaire. FGDs and interviews were also conducted in intervention schools with schoolchildren, parents, and teachers. Cambodia intervention knowledge and attitude scores improved by 19.2 (p < 0.001) and 25.3 (p < 0.001) percentage points, respectively, relative to the control. Laos intervention knowledge and attitude scores improved by 19.0 (p < 0.001) and 14.2 (p < 0.001) percentage points. However, Thailand’s intervention knowledge and attitude scores declined by 23.3 (p < 0.001) and 15.8 percentage points (p < 0.001). There were no improvements in behaviour scores in any country, but parents and schoolchildren in Cambodia and Laos reported improved fish preparation practices, suggesting positive spillover effects from MGO. The findings support MGO as an effective tool for school-based health education. Full article

Background: Parental burnout results from chronic stress related to the parental role and reflects a persistent imbalance between parenting demands and available psychological resources, negatively affecting parental well-being and parent–child relationships. This study examined the associations between parental burnout, parenting attitudes, and psychological resilience within the parental adaptation of the job demands–resources model, with particular attention to the potential mediating role of parenting styles in the relationship between resilience and parental burnout, while controlling for sociodemographic factors. Methods: A cross-sectional design was applied with 447 Hungarian parents who completed an anonymous online questionnaire including the Parental Burnout Assessment, the Parenting Styles and Dimensions Questionnaire, and the 10-item Connor–Davidson Resilience Scale. Data were analyzed using nonparametric correlations, group comparisons, multiple linear regression models with bootstrap estimation, and mediation analyses. Results: Resilience showed negative associations with all dimensions of parental burnout. Authoritarian and permissive parenting styles were positively associated with burnout, whereas authoritative parenting style showed negative associations. In multivariate analyses, authoritative parenting attitudes and fulfillment of the ideal parental role emerged as protective factors, while authoritarian parenting style functioned as a significant risk factor. Mediation analyses further indicated that the association between resilience and parental burnout may partly operate through parenting styles, particularly across the dimensions of emotional exhaustion, contrast, and emotional distancing. Conclusions: Parental burnout appears to be a dynamic psychological process shaped by the interaction of internal resources and parenting functioning, underscoring the importance of resource-oriented approaches in prevention and intervention. Full article

Background/Objectives: Dental fear and anxiety (DFA) in children can negatively affect oral health behaviors and dental care utilization. Family-related factors, particularly parental anxiety, parenting styles, and socioeconomic characteristics, may be associated with DFA. This study aimed to investigate the association between family-related factors and DFA among children aged 6–8 years in Jakarta, Indonesia. Methods: A cross-sectional study was conducted among 294 child–parent pairs recruited from 10 primary schools using multistage cluster sampling. Children’s DFA was assessed using the Children’s Fear Survey Schedule–Dental Subscale (CFSS-DS), whereas parental dental anxiety was measured using the Modified Dental Anxiety Scale (MDAS). Sociodemographic and family-related characteristics, including parenting styles, were collected using self-administered questionnaires. Data were analyzed using chi-square tests and multivariable logistic regression. Results: Overall, 34.7% of the children were classified as having DFA. Maternal employment was significantly associated with children’s DFA, with children of formally employed mothers having higher odds of DFA (aOR = 2.01, 95% CI: 1.05–3.85; p = 0.034). Parental dental anxiety was associated with children’s DFA. Children whose fathers and mothers reported high levels of dental anxiety had 4.68-fold (95% CI: 1.64–13.33; p = 0.004) and 2.50-fold (95% CI: 1.10–5.74; p = 0.029) higher odds of experiencing DFA, respectively. Dental drilling and injections were the most frequently reported fear-provoking stimuli. The final regression model explained 13% of the variance in children’s DFA. Conclusions: Parental dental anxiety and maternal employment were significantly associated with DFA among children aged 6–8 years. Family-centered preventive strategies and early identification of at-risk children may help reduce DFA and promote positive dental experiences and oral health outcomes. Full article

Chromosomal rearrangements that lead to the formation of oncogenic gene fusions, such as EML4-ALK, are thought to arise from incorrect repair of double-strand breaks in DNA. However, the mechanisms and factors driving rearrangement formation remain poorly understood, and analysis of these processes is limited by detection methods that are labor-intensive, low-throughput, and not readily quantitative at single-cell resolution. Here, we developed a genetically encoded ALK reporter based on A549 lung adenocarcinoma cells, created by inserting an ALK-P2A-mCherry cassette into the endogenous ALK locus, so that induced EML4-ALK fusion activated mCherry fluorescence. Reporter activation yielded a readily quantifiable mCherry-positive subpopulation that could be measured and enriched by flow cytometry and correlated with EML4-ALK levels. Using this platform, we combined CRISPR-mediated rearrangement induction with knockdown of DNA repair factors using RNA interference. Of the factors involved in base excision repair, homologous recombination-related pathways and canonical non-homologous end joining, knockdown of the APEX1 gene encoding apurinic endonuclease 1 (APE1) selectively increased EML4-ALK levels both in the reporter cell line and in parental A549 cells. Together, this work provides a sensitive, single-cell A549-based ALK reporter platform and a framework for future studies aimed at identifying cellular and environmental factors that modulate oncogenic EML4-ALK rearrangement formation. Full article

Background: Needle-related procedures such as venipuncture can be distressing for children and may trigger severe fear and behavioral dysregulation, particularly in those with previous traumatic experiences. Trauma-informed care (TIC) is a framework that recognizes the widespread impact of trauma and integrates this knowledge into clinical practice to prevent re-traumatization and support emotional regulation during medical procedures. Methods: This before-and-after study included 135 children aged 4–8 years who had previously shown severe distress during venipuncture, including escape attempts, shouting, or self/other-directed aggressive behaviors. Before venipuncture, children and their families received a TIC-based intervention delivered by a psychological counselor in a dedicated preparation room. Fear, behavioral responses during venipuncture, procedural pain, and heart rate were evaluated before and after the intervention using parent reports, the Children’s Fear Scale, the Wong–Baker FACES Pain Rating Scale, and pulse oximetry. Results: Following the TIC intervention, significant pre–post reduction were observed in distress-related behaviors during venipuncture, including escape attempts, shouting/crying, and self-/other-directed harmful behaviors. The proportion of children rated as experiencing high levels of fear decreased from 96.2% before the intervention to 15.5% after. Among the 85 children with complete heart-rate measurements available, mean heart rate decreased from 113.6 ± 10.1 beats/min to 87.3 ± 8.43 beats/min. Many families reported a more positive venipuncture experience compared with previous procedures. Conclusions: A trauma-informed care intervention delivered before venipuncture is associated with meaningful reductions in behavioral distress, fear, and physiological arousal in children with prior needle-related traumatic experiences. These pre–post associations support the feasibility and potential value of the TIC model, though controlled studies are needed to confirm these findings without confounding clinical effects. Full article

Several countries have adopted a nationwide ban on social media access for children. Our aim was to investigate public opinion regarding the implementation of a social media ban for children, as well as the factors influencing these views. We measured agreement with the ban, information regarding its implementation, perceived need for additional measures, confidence in the effectiveness of the ban, perceived impact of the ban, and parental familiarity with digital parental control tools. The study sample included 619 participants. In our sample, 69% agreed with the implementation of the ban, while 86.5% believed that additional measures should be implemented (i.e., digital literacy courses in schools, active parental involvement in digital literacy, prohibition of inappropriate content, reasonable parental limits on social media use, and restriction of addictive platform features). Females and higher-educated participants had more positive perceptions regarding the impact of the ban. We found a positive association between age, financial status, social media use, and impact of the ban. Reduced age was associated with increased parental familiarity with digital parental control tools. Social media use was associated with parental familiarity with digital parental control tools. There is a need for holistic and evidence-informed policy frameworks that integrate regulatory measures, educational initiatives, and shared accountability among stakeholders. Full article

Schistosomiasis remains a major public health concern in Nigeria. We molecularly characterized Schistosoma eggs obtained from human urine to identify species and assess the presence of hybrid schistosomes in Abuja, Nigeria. Urine samples were collected from 1887 participants aged five years and above. Samples were examined for Schistosoma eggs using light microscopy. A total of 507 (26.9%) were positive for any form of Schistosoma while 91 (4.8%) had atypical Schistosoma eggs. DNA extracted from pooled ova was analyzed using metagenomic sequencing, read mapping, phylogenetic analysis, and BLASTn confirmation. Molecular analyses identified genetic signatures associated with both S. haematobium and S. bovis within pooled human urine samples, indicating the co-circulation of multiple schistosome species in the study area. Phylogenetic analyses based on trans-ITS and mitochondrial COX1 markers supported the presence of distinct nuclear and mitochondrial schistosome lineages. However, because sequencing was performed on pooled egg samples, the findings cannot distinguish between true recombinants and mixed infections involving co-circulating parental species. These findings highlight the potential complexity of schistosome transmission dynamics in endemic communities and underscore the need for enhanced molecular surveillance, especially single-parasite genomic approaches, and integrated One Health investigations to better understand schistosome transmission and its implications for control and elimination efforts in Nigeria. Full article

Background: The paediatric dentistry domain requires effective management of pain in children during invasive procedures such as the inferior alveolar nerve block (IANB). This study aimed to evaluate the effectiveness of Buzzy BEE^TM in reducing pain perception during IANB in children. Methods: This crossover study recruited 38 children aged 6–10 years scheduled for bilateral dental procedures requiring IANB. The washout period was 7 days, and two visits were scheduled for procedures on either side. Two randomly allocated groups received the study intervention, with each group receiving it alternately at each visit. Outcomes measured were pulse rate; subjective pain assessment using the Wong–Baker FACES Rating Scale (WBS); objective pain assessment using the Sound, Eyes and Motor (SEM) scale; and parental rating of observed pain on a scale of 1–10. Results: A statistically significant reduction in pulse rate after IANB was observed in the Buzzy BEE group at the first (p = 0.02) and second (p = 0.002) visits. At the second visit, the WBS scores (p < 0.001) and ‘eye’ (p = 0.004) and ‘motor’ (p = 0.002) scores on the SEM scale were significantly reduced in the Buzzy BEE group. The crossover analysis identified a significant treatment effect on pulse rate (p < 0.001) and significant carryover effects on WBS and SEM scores (p < 0.001). Conclusions: The use of Buzzy BEE reduced pulse rates during IANB, suggesting a positive impact on children’s anxiety. The carryover effects in the current study limit the consistency of improvements in subjective and objective pain perceptions. Further studies with a larger sample size and an extended washout period are recommended to evaluate the effectiveness of Buzzy BEE in reducing pain perception during IANB. Full article

In the Iberian Peninsula, the rapid expansion of the invasive species Alburnus alburnus (bleak) has intensified its contact with several endemic cyprinid species, raising concerns about hybridization, introgression, and the loss of genetic diversity. Despite increasing evidence of hybridization, data remain limited for many Iberian River basins, where endemic species persist in fragmented and vulnerable habitats. The aim of this study is to assess the extent and spatial distribution of hybridization between the bleak and the following native cyprinid species, Anaecypris hispanica (jarabugo), Iberochondrostoma lemmingii (pardilla), Pseudochondrostoma willkommii (Guadiana nase), and Squalius alburnoides (calandino), across several rivers within the Guadalquivir Basin. To this end, Sanger sequencing will be performed on the mitochondrial cytochrome b gene (maternal lineage; approximately 1000 base pairs (bp)) and the nuclear β-actin gene (paternal lineage; approximately 950 bp) from individuals of all endemic species and the bleak. Parental species and putative hybrids were initially identified in the field using diagnostic morphological and meristic characters, including number of rays in the anal fin morphology, mouth position, and the number of lateral line scales. Molecular analyses will include haplotype network reconstruction and phylogenetic tree inference to evaluate relationships among individuals from different species and to assess lineage divergence. The results will allow us to: (1) detect hybrids between the bleak and endemic cyprinids, (2) identify hybridization events among endemic cyprinid species, and (3) evaluate the correspondence between diagnostic morphological and meristic traits and the molecular identification of hybrid individuals. Overall, these findings will provide key information for the conservation management of endangered Iberian freshwater fishes in the context of invasive species expansion and global change. Full article

Tetracycline-class antibiotics are persistent contaminants in aquatic environments and are difficult to remove by conventional treatment processes. In this study, a recoverable granular MIL-101(Fe)/γ-Al₂O₃ catalyst was prepared through ligand anchoring followed by secondary Fe-MOF growth on spherical γ-Al₂O₃ and applied to catalytic ozonation of tetracycline (TC) under ordinary-bubble and microbubble-assisted operation. Structural characterization supported the formation of Fe-containing MOF domains on the alumina support, accompanied by an increase in BET surface area from 164.28 to 210.05 m² g⁻¹ and enhanced Lewis-acid-related pyridine-IR signals. Under conventional bubbling ozonation, the optimized catalyst achieved 67.93% apparent UV–Vis-based TC removal during an overall 50 min run consisting of 30 min dark adsorption followed by 20 min ozonation. In a 12 L microbubble reactor, the catalyst-assisted system reached 93.74% apparent UV–Vis-based TC removal at pH 6 with 100 g catalyst and 6 mg min⁻¹ fed ozone, showing higher apparent removal than ordinary ozonation, microbubble ozonation, and ordinary-bubble catalytic ozonation under the tested configuration. Phosphate-blocking and radical-quenching experiments were consistent with the involvement of Lewis-acid-related sites, hydroxyl radicals, and superoxide-related pathways, but these tests are interpreted as indirect mechanistic evidence. LC-MS analysis suggested possible hydroxylation, demethylation, deamidation, ring opening, and low-molecular-weight product formation. The system also transformed chlortetracycline, oxytetracycline, and doxycycline and reduced COD and TOC in a simulated mixed-antibiotic matrix. Because parent-compound HPLC/LC-MS time-series quantification, ozone utilization/off-gas ozone measurement, bubble-size/kLa analysis, and ICP-based Fe loading/leaching data were not available, the present work is positioned as an apparent catalyst–reactor coupling study rather than a complete catalytic, hydrodynamic, or process-level demonstration. Full article

Play is a universal activity. Yet there are cultural and linguistic differences in how families engage in adult–child play. In the present study, Thai–English bilingual mother–child dyads completed a toy play task in both languages. The results revealed cross-linguistic differences in bilingual mothers’ and children’s conversation styles. When speaking Thai, the nature of bilinguals’ dyadic play was more adult-centered, characterized by the use of directives by the mothers and use of repetitions by the children, which was congruent with parent–child interpersonal dynamics in high-power-distance Asian cultures. When speaking English, the play session was more child-centered, evidenced by children’s use of directives and encouragements, which was congruent with behavioral norms in low-power-distance Western cultures. Bilingual mothers and children exhibited positive associations in their narrative styles during both the Thai and English sessions. Additionally, the preliminary results provided evidence that cross-linguistic differences in mother–child speech patterns may be moderated by child gender. These findings suggest that the communicative and interactional patterns that bilingual caregivers modeled for bilingual children varied across languages and that preschoolers aligned their behaviors with those exemplified by their mothers. We conclude that bilingualism influences early social communication, with theoretical and applied implications for researchers, educators, and clinicians. Full article

Background: Parenting stress is a known risk factor for children’s social anxiety, yet the mediating and moderating mechanisms underlying this relationship remain underexplored, particularly regarding gene–environment interactions. This quantitative, cross-sectional study, grounded in diathesis-stress and family process theories, examined whether maladaptive parenting mediates the link between parenting stress and children’s social anxiety, and whether FKBP5 gene variation moderates this mediation. Methods: A sample of 1774 fourth- to sixth-grade students (aged 10–14 years) and their parents participated. Parenting stress and maladaptive parenting were parent-reported, children’s social anxiety was self-reported, and children’s FKBP5-related cumulative genetic score was derived from four SNPs (rs4713916, rs1360780, rs3800373, rs9296158). Moderated mediation analyses were conducted. Results: Parenting stress was significantly and positively associated with children’s social anxiety. Maladaptive parenting partially mediated this relationship. The FKBP5 showed a marginally significant moderating effect, with simple slope analysis suggesting parenting stress was more strongly associated with child social anxiety among children with higher genetic risk. No moderating effect was found for the path from maladaptive parenting to social anxiety. Conclusions: Parenting stress is associated with children’s social anxiety both directly and indirectly through maladaptive parenting, with FKBP5-related cumulative genetic risk potentially moderating the direct effect. These findings offer preliminary evidence that may inform preventive interventions targeting parenting stress, although replication is needed. Full article

Using theories of Afrocentricity (African Indigenous Knowledge) and of positioning, we investigated how Sub-Saharan African immigrant parents (SSAIP) support their Grade 6–9 children’s mathematics learning in Canada. Individual interviews were collected from twelve immigrant parents living in an urban community in Alberta and new to Canada (within five years). We ask: what are the mathematics experiences and perspectives of Sub-Saharan African immigrant families? We analyzed interview data from 12 SSAIPs from Greater Edmonton, Canada to identify the storylines they shared regarding their experiences and perspectives of mathematics learning. The prevailing storylines were interpreted through an African Indigenous Knowledge lens. We found that parents adjust their mathematics learning support for their children by negotiating their experiences from two cultural worlds of education: pre-colonial (African Indigenous Knowledge) and colonized worldviews, their home and host cultures, generally. We identified these storylines, some of which sit in tension with each other: “mathematics learning is a communal responsibility”, “mathematics teachers share responsibility for the moral upbringing of the child”, “mathematics as memory work is feared and stressful”, “adults tell children what to do”, “adults negotiate with children about what to do”, and “success in mathematics is a gift from God”. We interpreted the storylines through the Akan Adinkra epistemologies which manifest in the Adinkra symbols and sayings, and through our experiences living and working in Sub-Saharan Africa, to determine the way each storyline aligned with old African or new African ways. This study will be beneficial for educators wishing to adopt culturally responsive ways of engaging immigrant families in their children’s mathematics learning. Full article