Search Results (1,052)

The aquatic plant Wolffia globosa and the probiotic Limosilactobacillus reuteri KUB-AC5 have been proposed as candidate synbiotic components targeting gut microbiota modulation. In this study, we investigated the effects of W. globosa supplementation, alone or in combination with L. reuteri KUB-AC5, on the gut microbiota and health status in healthy adult dogs. For a 28-day feeding trial, 24 healthy dogs were randomly assigned to three dietary treatment groups: control, prebiotic (PRE; 3.5% W. globosa powder), and synbiotic (SYN, 3.5% W. globosa powder combined with L. reuteri KUB-AC5 at 1 × 10⁸ CFU/day). No significant differences were observed in body weight, body condition score, fecal consistency, feed intake, or blood parameters among groups (p > 0.05). Although overall gut microbial diversity remained unchanged, linear discriminant analysis effect size analysis revealed the selective enrichment of specific bacterial taxa. The PRE group exhibited higher relative abundances of Parabacteroides merdae, Romboutsia lituseburensis, Subdoligranulum variabile, and Phocaeicola sartorii, whereas the SYN group showed increased levels of Escherichia coli and Terrisporobacter glycolicus and reduced levels of Anaerobiospirillum succiniciproducens and Campylobacter upsaliensis (p < 0.05). In conclusion, W. globosa, alone or combined with L. reuteri KUB-AC5, was safely used to modulate gut bacterial composition as a potential functional ingredient. Full article

Celery (Apium graveolens), rosehip (Rosa canina), and blackcurrant (Ribes nigrum) powders (1–3%, w/w) were evaluated in sous-vide pheasant meat prepared with or without 1.8% NaCl and stored for 28 days at 4 °C. Measurements included proximate composition, pH and titratable acidity, instrumental texture and color, FRAP, ABTS, Folin–Ciocâlteu-reactive compounds, TBARS, nitrate/nitrite, chemical-spoilage screening, and microbial counts; sensory quality was assessed descriptively by a laboratory panel on day 28. Mean cooking loss was 22.11% in salted and 24.10% in unsalted samples. At day 28, 3% rosehip and blackcurrant formulations had total viable counts of 3.80–4.16 log₁₀ CFU/g, compared with 5.78–6.28 log₁₀ CFU/g in controls. Their FRAP values were 2.83–3.23 µmol TE/g dry matter and TBARS values were 0.43–0.57 mg MDA/kg, compared with 1.00–1.05 µmol TE/g dry matter and 1.45–1.53 mg MDA/kg in controls. Celery at 3% retained approximately 170 mg/kg nitrate and 13.3–13.4 mg/kg nitrite. Rosehip and blackcurrant, particularly at 3%, provided the most favorable combination of measured quality attributes under the tested conditions. These findings demonstrate ingredient-specific effects but do not validate pathogen control, commercial shelf life, or population-level consumer preference. Full article

This study investigated whether salinity during cultivation of the aquatic plant Lemna minor (duckweed) influences the phytochemical composition of plant extracts and the properties of green-synthesized zinc oxide nanoparticles (ZnO NPs). Duckweed was cultivated under 0, 10, and 100 mM NaCl, followed by Orbitrap metabolomic profiling, nanoparticle synthesis, physicochemical characterization, and evaluation of antioxidant and antimicrobial activities. Orbitrap analysis revealed pronounced salinity-dependent changes in extract composition, including increased abundance of several flavonoids, glycosylated flavones, and hydroxycinnamic acid derivatives in the order 0 < 10 < 100 mM. ZnO nanoparticle formation was supported by UV–Vis spectroscopy, which showed characteristic absorption features around 360 nm, and by powder X-ray diffraction (PXRD), which indicated the predominance of the hexagonal wurtzite ZnO phase in all samples. SEM–EDS analysis revealed Zn- and O-rich materials consisting of micron-scale aggregates and finer submicron structures. Raman spectra were dominated by fluorescence, which increased with salinity treatment and may reflect differences in surface-associated phytochemicals rather than substantial changes in the ZnO crystal structure. Nanoparticles synthesized using extracts from salt-stressed duckweed exhibited higher total phenolic content (up to 66.79 ± 0.15 µM GAE g⁻¹), antioxidant activity (up to 55.01 ± 0.21%), and antimicrobial activity against Staphylococcus haemolyticus D4-2-100/1 (inhibition zone up to 1.55 ± 0.05 cm). Although the mechanisms underlying these differences remain to be fully elucidated, the results suggest that salinity-induced changes in duckweed metabolism may influence the biological properties of the resulting nanomaterials. Overall, this study highlights the potential of manipulating cultivation conditions to modulate plant extract composition and, consequently, influence the characteristics and functionality of green-synthesized ZnO nanoparticles. Full article

This study investigates citrus peel powder (CP) as an environmentally friendly admixture to regulate plant-derived urease (with soybean powder (SP) as the urease source) and to promote bio-mediated CaCO₃ mineralization, thereby improving the mechanical and freeze–thaw (FT) resistance properties of cement-based materials. When CP is combined with urea and soybean urease, it exhibits a regulatory effect on urease activity. For the CPUD (CP-encapsulated urea combined with soy powder)-modified material with SP dosage in cement content of 0.2 wt%, the CP–urea modification treatment can effectively improve their mechanical properties and FT durability. The flexural and compressive strengths at 28 days are increased by 10.53% and 11.19%, respectively, compared to the blank group. After freeze–thaw cycles, the strengths are still 27.08% and 26.67% higher than those of the blank group, and their respective strength loss rates are 7.58% and −5.77% (negative indicating a net strength increase), compared with 21.31% and 9.48% for the blank group. X-ray diffraction, Fourier-transform infrared spectroscopy, and scanning electron microscopy analyses reveal that CP–urea promotes the formation and effective packing of calcium carbonate. Mechanistically, CP establishes a stable hydrogen-bonding network with both urea and urease, exerting a dual regulatory effect: it enhances the electrophilicity of urea while also creating a physical mass transfer barrier to precisely control biomineralization. Notably, CP can be directly used without pretreatment, offering a sustainable strategy for citrus peel waste valorization. Full article

This study aimed to evaluate how the dosage, type of herbal preparation (plant material, dry extract, or isolated compound), along with simulated digestion-related factors, i.e., digestion stage (gastric vs. intestinal) and corresponding physicochemical and biochemical environment, influence the in vitro bioaccessibility of lemon balm phytochemicals. Simulated in vitro digestion revealed that the bioaccessibility of rosmarinic acid (RA), total phenolics, and antioxidant activity strongly depended on the application form, dose, and digestion stage. Isolated RA exhibited the highest gastric in vitro bioaccessibility, which markedly decreased during intestinal digestion. Dry extracts provided consistently higher in vitro bioaccessibility of RA, TPC (Total Phenolic Content), ABTS (ABTS scavenging activity), and FRAP (Ferric Reducing Antioxidant Power) than powdered plant material, particularly at doses of ≥25 mg. Intestinal digestion enhanced the in vitro bioaccessibility of phenolics and antioxidant activity from plant material under physicochemical conditions, whereas biochemical digestion factors generally reduced in vitro bioaccessibility, especially at lower doses. Overall, higher doses ensured more stable in vitro bioaccessibility across all analysed parameters. Full article

In arid and semi-arid regions, mesquite (Prosopis laevigata) pods represent a strategic feed resource for low-input livestock systems; however, the utilization of mesquite pod flour is severely limited by insect infestation during storage. This study evaluated the repellent effectiveness of Ricinus communis leaves and stems as plant-based additives for preserving mesquite pod flour against storage insects, primarily Tribolium castaneum (Coleoptera: Tenebrionidae). Dose-dependent repellency was assessed in a three-chamber choice-test system at concentrations of 1, 5, 10, and 20% (w·w⁻¹), combining short-term laboratory assays with a 14-month storage experiment under ambient conditions representative of rural dryland systems. R. communis leaves exhibited a strong concentration-dependent repellent effect, consistently outperforming stem material. Logistic regression indicated that each 1% increase in leaf concentration increased the odds of insects remaining in the untreated substrate by 17%. Long-term storage assays showed that botanical additives altered insect population structure, while only R. communis leaf powder at 10% showed lower total insect abundance relative to the control. Proximate analysis revealed additional protein contribution from R. communis leaves, while ricin-like proteins remained at low concentrations in vegetative tissues. Overall, the results validate a traditional preservation practice and support the use of R. communis leaf biomass as a plant-based additive for protecting mesquite flour in dryland livestock systems. Full article

Tritium breeding blanket is a key component of future fusion power plants, and its performance depends on the selection, fabrication, and qualification of lithium-based ceramic material. Among the proposed lithium ceramics materials, the main candidates for ceramic breeders are lithium orthosilicate (Li₄SiO₄) and lithium metatitanate (Li₂TiO₃). These advanced ceramics and their biphasic composites are the leading candidates due to their high lithium density, favorable tritium breeding ratio (TBR ≈ 1.15–1.25 with Be₁₂Ti multiplier and 90% ⁶Li enrichment), and robust thermo-mechanical behavior within the 200–900 °C operational window of helium-cooled pebble bed (HCPB) blankets. This review provides an engineering-oriented assessment covering fabrication routes (solid-state, hydrothermal, melt-based, drip casting, powder injection molding, microwave sintering, and digital light processing additive manufacturing); microstructure–property relationships and performance under neutron irradiation; and tritium generation, retention, and release as functions of chemical composition, defect structure, and operating temperature. Induced radioactivity of Li-based ceramics and key impurity elements is quantified using activation formalisms applied to WWR-K reactor conditions, providing guidance for raw-material selection and waste-management assessment. Authors’ original contributions include (i) an empirical model of pebble crush load vs. biphasic composition (R² > 0.99); (ii) two universal semi-empirical kinetic models (exponential growth and non-linear strength degradation, R² = 0.97–0.99) for nine structural and mechanical parameters of Li₂TiO₃ under He²⁺ and H⁺ irradiation; (iii) a consolidated table of Arrhenius tritium diffusion parameters from reactor experiments and DFT; and (iv) an induced radioactivity calculation for the biphasic system with two-exponential post-irradiation decay analysis. The review identifies biphasic Li₄SiO₄–Li₂TiO₃ composites with ~30 ± 5 mol.% Li₂TiO₃ as particularly promising and formulates specific data gaps and modeling needs for the reliable deployment of ceramic breeder pebbles in helium-cooled fusion blanket systems. It should be specifically noted that Li₄SiO₄ pebbles fabricated via the melt method, as an example, typically exhibit exceptionally high densities, generally exceeding 90% of the theoretical density (TD). Building on the calculation of induced radioactivity, it is crucial to consider the microstructural distribution of highly radioactive nuclides (e.g., Co, Mn) within the ceramic matrix. If these impurities segregate at grain boundaries rather than being homogeneously distributed, there is a potential pathway to develop targeted wet-chemical methods, such as selective acid leaching, to remove these impurities post-irradiation, thereby lowering the waste disposal classification. Full article

Oat milk (OM) has gained popularity as a plant-based dairy alternative; however, conventional filtration-based production removes oat pulp, leading to β-glucan loss and processing waste. This study investigated the effects of particle size reduction by different grinding techniques on the powder properties and in vitro digestion characteristics of oat powder (OP), and it evaluated the applicability of superfine OP to filtration-free OM production. OP was prepared at three particle sizes: coarse, fine, and superfine, using a blender, ultra-centrifugal mill, and ball mill, respectively. Decreasing particle size improved hydration properties, including water absorption capacity, swelling capacity, and water solubility. During in vitro digestion, OP-superfine showed higher dialyzable protein fraction, β-glucan extractability, and digestion extract viscosity than OP-coarse, indicating an enhanced release of proteins and viscosity-contributing soluble components. When applied to OM, OP-superfine increased viscosity, Brix, turbidity, and suspension stability, while particle size had only a minor influence on pH. Sensory evaluation showed that OM prepared with OP-superfine had reduced grittiness and throat-feel intensity while maintaining relatively high sweetness. These findings suggest that superfine grinding is a promising strategy for producing filtration-free OM with improved digestion-related properties, physical stability, and sensory quality. Full article

Excess phosphate in aquatic environments can trigger eutrophication and pose risks to ecosystem integrity and public health, even though phosphate is indispensable for plant growth. Herein, we report the fabrication of Zr-LDHs-PS hydrogel microspheres by in situ cross-linking zirconium–magnesium–iron layered double hydroxides (ZrMgFe-LDHs) with Polyvinyl alcohol (PVA) and sodium alginate (SA). The resulting bead-type adsorbent was designed to enable efficient phosphate capture from water while facilitating subsequent, controlled phosphate release. Benefiting from the cross-linking granulation strategy, the microspheres mitigate typical limitations of powdered adsorbents, including compaction, aggregation, and poor separability. General characterization (SEM, FT-IR, XPS, XRD, BET, TG, and zeta potential) elucidated the microstructure and surface chemical composition. The Zr-LDHs-PS microspheres exhibited a maximum experimental adsorption capacity of 51.313 mg/g. Kinetics data were best fitted by the pseudo-second-order model, and adsorption isotherms were subjected to the Freundlich model, pointing to heterogeneous, multilayer adsorption. Importantly, high phosphate selectivity was preserved despite the coexistence of competing anions (Cl⁻, NO₃⁻, and CO₃²⁻). After adsorption, the spent beads released phosphate gradually in water, highlighting their potential for dual functionality. Collectively, these results demonstrate that Zr-LDHs-PS hydrogel microspheres are promising candidates for extraction-based phosphate removal and resource recovery, with prospects for repurposing slow-release phosphate fertilizers to support sustainable plant nutrition. Full article

Although the sublethal effects of botanical pesticides are critical for pest management, their role in modifying insect behaviour is frequently overlooked. In a laboratory bioassay utilising powder prepared from the leaf, stem, and root of the invasive alien plants (IAPs) Gleditsia triacanthos, Chromolaena odorata, and Schinus terebinthifolia, this study evaluated the behavioural reactions, notably repellence and locomotion, of Spodoptera frugiperda larvae. For repellence, six second instar larvae of S. frugiperda were individually placed in 30 mL vial that remained unsealed to allow larvae to exit freely if repelled and were exposed to 22% of leaf, stem and root powders of all three plant species over a 12 h period before recording the number of repelled insects. For locomotion, ten-second instar larvae were directly coated with different powder quantity 1.5 g and 2.5 g of leaf, stem, and root of all three IAPs, then released and allowed to move for 60 s on a 90 × 60 cm whiteboard. The running speed was calculated as the distance travelled per unit time. All plant-part powder exhibited significantly higher repellence than the control (0%). Gleditsia triacanthos leaf, root and stem powders showed minimal repellence, with values of 16%, 29% and 21%, respectively. However, higher rates were observed for C. odorata and S. terebinthifolia. Specifically, C. odorata stem powder achieved the highest overall repellence at 54%, whereas S. terebinthifolia performed best with its root powder at 45%. Compared to the control group, the running speed of the insects experienced a substantial reduction, decreasing by 60 to 80% when exposed to powders derived from all parts of the three IAPs. Our results suggest that the IAP powders used in this study both repel S. frugiperda and strongly limit their ability to move, highlighting their potential for behaviour-based pest control strategies. Full article

This study examined the impact of varying addition levels (0%, 0.5%, 1%, 2%, and 4%) of electro-processed grape seed powder on the physicochemical properties and gel features of chicken myofibrillar protein. The incorporation of modified grape seed powder (MGSP) with an alternating electric field can strengthen the structure of myofibrillar protein (MP) gel, as well as improve its water retention capacity and solubility to a degree. Moreover, as the amount of addition increases, the gel’s hydrophobicity decreases and the number of carbonyl groups decreases, but the antioxidant activity improves. Ionic bonds and disulphide bonds are predominant intermolecular forces in the MP gel. MGSP can induce alterations in protein conformation and enhances the development of a dense and homogeneous gel network. Overall, when the addition amount is 2–4%, the gel characteristics reach optimal conditions. This study offers a crucial informational foundation for the utilization of plant-based materials altered by alternating electric fields and composite gels in meat products. Full article

Callosobruchus maculatus (Fabricius, 1775) is a major postharvest pest of stored legumes, causing significant quantitative and qualitative losses. This study evaluated host preference, biochemical determinants of susceptibility, and the insecticidal efficacy of plant powders and aqueous and ethanolic extracts against C. maculatus among selected pulse species. In free-choice assays, oviposition preference did not correspond with developmental success, as Phaseolus vulgaris Linnaeus, 1753, received the highest number of eggs but supported minimal adult emergence, whereas Vigna radiata (Linnaeus) A. Jussieu, 1954, exhibited the highest level of infestation and seed damage. Biochemical analysis revealed correlational patterns: higher carbohydrate and lipid contents were positively associated with infestation and seed weight loss, whereas fiber content showed a negative association with pest performance. Protein content was correlated with oviposition, while moisture and fiber were associated with reduced developmental success. Plant-derived treatments significantly affected all biological parameters of C. maculatus in a concentration- and time-dependent manner, with the exception of sex ratio. Among the tested botanicals, Azadirachta indica A. Jussieu (1830) consistently showed the highest toxicity, with the lowest LC₅₀ values across exposure periods. Ethanolic extracts showed greater insecticidal activity than aqueous extracts, indicating solvent-dependent extraction of bioactive compounds. Mortality increased progressively with exposure duration, accompanied by decreasing LC₅₀ values, suggesting cumulative toxic and developmental effects. Overall, the results demonstrate that host suitability is governed by the interaction between nutritional composition and structural seed traits, while botanical extracts, particularly those from A. indica, offer effective, eco-friendly alternatives for managing C. maculatus in stored legumes. Full article

Background/Objectives: Latent fingerprints are routinely recovered from conventional porous and non-porous substrates; however, biologically active surfaces such as plant leaves are generally regarded as unsuitable for dactyloscopic evidence. Because vegetation is frequently present at crime scenes, this study aimed to systematically evaluate whether plant leaves can retain usable friction ridge detail and to determine the durability and forensic value of such traces under laboratory and outdoor conditions. Methods: Latent fingerprints were deposited on leaves of multiple plant species (maple, ash, dandelion, bird cherry, chestnut, climbing ivy, and five-leaved ivy) under dry and hydrated conditions and at defined time intervals after deposition. Visualization was performed using several powders, with SupraNano Fluorescent Green magnetic powder providing the best performance. Developed impressions were photographed using controlled illumination and evaluated using automated quality assessment (NFIQ 2.0) and comparison software (Innovatrics IDkit 9.1.7.1004). Additional experiments examined living, growing leaves exposed to natural weather conditions for extended periods. Results: Usable ridge detail was successfully visualized on all tested species. Bottom leaf surfaces and hydrated samples generally provided better preservation and contrast. Identifiable traces persisted for up to 20 h on detached leaves and for up to 35 days on living leaves despite growth-related deformation. Under outdoor exposure, fingerprints on ivy remained visible and comparable for up to 60 days. Although overall automated quality scores were reduced by background venation, selected impressions achieved measurable comparison scores and successful matches. Conclusions: Plant leaves can serve as unconventional yet viable carriers of latent fingerprints. Magnetic fluorescent powder development combined with careful documentation enables recovery of forensically useful ridge detail even after prolonged environmental exposure. These findings expand the range of substrates that should be considered during crime scene processing and provide practical guidance for evidence collection on vegetation. Full article

Young barley, derived from the early vegetative stage of Hordeum vulgare L., constitutes a plant-based functional ingredient whose phytochemical profile differs markedly from that of mature grain. Two principal commercial forms exist—dried grass powder and juice-derived products—differing in matrix composition and bioactive compound concentration. This narrative review critically evaluates the current knowledge on the phytochemical composition, biological activity, and translational relevance of young barley preparations considered as a functional plant food. The phytochemical spectrum is dominated by C-glycosyl flavones, particularly saponarin and lutonarin, alongside phenolic acids, chlorophylls, enzymatic antioxidants, vitamins, and minerals. Experimental evidence implicates the modulation of redox homeostasis, inflammatory signaling, and metabolic regulators as the primary biological mechanisms. In vitro studies additionally demonstrate antiproliferative activity in human cancer cell lines and immunomodulatory properties mediated by polysaccharide-rich fractions, extending the biological profile of young barley beyond classical antioxidant activity. Although preclinical models consistently demonstrate antioxidant and metabolic effects, high experimental doses and limited preparation standardization restrict the direct extrapolation to human supplementation contexts. Available clinical trials suggest modest improvements in selected lipid, glycemic, and oxidative stress markers; yet, most are small in scale and brief in duration. Agronomic variables including fertilization strategy and soil composition represent additional, underappreciated sources of phytochemical variability and safety concern. Overall, the current evidence supports the biological plausibility of young barley as a functional plant food; yet, the clinical data remain preliminary. Future research should prioritize preparation standardization, dose–response characterization, and agronomic transparency to strengthen translational reliability. In conclusion, young barley preparations represent a biologically plausible functional plant food ingredient with preliminary clinical support, pending confirmation from adequately powered, standardised randomised controlled trials. Full article

Background: Instant Cascara (IC) beverages, derived from dried coffee cherry pulp, represent an upcycled plant-based ingredient rich in phenolic compounds and methylxanthines. Although spray-drying enables the production of soluble cascara powders without carriers, previous sensory evaluation highlighted limitations in palatability, supporting the need for formulation strategies. Objective: To evaluate how the incorporation of inulin-type carriers with different degrees of polymerization modulates production yield, the apparent recovery of bioactive compounds, and formulation-dependent in vitro biological and receptor-level responses of Instant Cascara beverages. Methods: Formulations without carrier (IC 0.0) and with long-chain inulin (IC 1.0) or oligofructose-enriched inulin (IC 2.0) were prepared and characterized. Production yield, phytochemical composition, and in vitro antioxidant, anti-inflammatory, antiproliferative, and receptor-mediated responses were assessed using analytical tools, cell-based assays, and receptor-based platforms. Results: Carrier incorporation improved production yield, particularly for IC 1.0. Although differences in apparent recovery of bioactive compounds were observed, all formulations preserved relevant in vitro biological activities. IC 2.0 showed stronger nitric oxide inhibition and apoptosis induction in colorectal cancer cell models. Receptor-based assays revealed formulation-dependent differences, including reduced activation of bitter taste receptors (TAS2Rs), absence of sweet receptor (TAS1R2/TAS1R3) activation, and modulation of muscarinic (M3) and dopaminergic (D3/D4) receptor responses. These effects are consistent with variations in the composition and effective concentration of bioactive compounds between formulations, particularly caffeine. Conclusions: The incorporation of inulin-type carriers influences production yield and modulates in vitro biological responses and receptor-level responses of Instant Cascara beverages. IC 2.0 represents a formulation with a favorable balance between technological performance and functional responses, associated with a distinct receptor-level profile. This balance may be related to a reduced contribution of bitterness-associated compounds, such as caffeine, together with the preservation of other bioactive components contributing to the observed biological responses. These findings provide a mechanistic in vitro basis for future sensory and in vivo studies evaluating how formulation-dependent differences in bioactive composition may influence physiological responses and consumer perception. Full article