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18 pages, 9273 KB  
Article
Cross-Scanner Harmonization of AI/DL Accelerated Quantitative Bi-Parametric Prostate MRI
by Dariya Malyarenko, Scott D. Swanson, Jacob Richardson, Suzan Lowe, James O’Connor, Yun Jiang, Reve Chahine, Shane A. Wells and Thomas L. Chenevert
Sensors 2025, 25(18), 5858; https://doi.org/10.3390/s25185858 - 19 Sep 2025
Viewed by 229
Abstract
Clinical application of AI/DL-aided acquisitions for quantitative bi-parametric (q-bp)MRI requires validation and harmonization across vendor platforms. An AI/DL-accelerated q-bpMRI, including 5-echo T2 and 4-b-value apparent diffusion coefficient (ADC) mapping, was implemented on two 3T clinical scanners by two vendors alongside the qualitative [...] Read more.
Clinical application of AI/DL-aided acquisitions for quantitative bi-parametric (q-bp)MRI requires validation and harmonization across vendor platforms. An AI/DL-accelerated q-bpMRI, including 5-echo T2 and 4-b-value apparent diffusion coefficient (ADC) mapping, was implemented on two 3T clinical scanners by two vendors alongside the qualitative standard-of-care (SOC) MRI protocols for six patients with biopsy-confirmed prostate cancer (PCa). AI/DL versus SOC bpMRI image quality was compared for MR-visible PCa lesions on a 4-point Likert-like scale. Quantitative validation and protocol bias assessment were performed using a multiparametric phantom with reference T2 and diffusion kurtosis values mimicking prostate tissue ranges. Six-minute q-bpMRI achieved acceptable diagnostic quality comparable to the SOC. Better SNR was observed for DL/AI versus SOC ADC with method-dependent distortion susceptibility and resolution enhancement. The measured biases were unaffected by AI/DL reconstruction and related to acquisition protocol parameters: constant for spin-echo T2 (−7 ms to +5 ms) and ADC (4b-fit: −0.37 µm2/ms and 2b-fit: −0.19 µm2/ms), while nonlinear for echo-planar T2 (−37 ms to +14 ms). Measured phantom ADC bias dependence on b-value range was consistent with that observed for PCa lesions. Bias correction harmonized lesion T2 and ADC values across different AI/DL-aided q-bpMRI acquisitions. The developed workflow enables harmonization of AI/DL-accelerated quantitative T2 and ADC mapping in multi-vendor clinical settings. Full article
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19 pages, 4015 KB  
Article
New Geochemical Insights into Pre-Khorat Paleoenvironments: A Case Study of Triassic–Jurassic Reddish Sedimentary Rocks in Thailand
by Vimoltip Singtuen, Burapha Phajuy and Punya Charusiri
Geosciences 2025, 15(8), 324; https://doi.org/10.3390/geosciences15080324 - 19 Aug 2025
Viewed by 676
Abstract
The Nam Phong Formation, a key unit of the pre-Khorat Group in the western Khorat Plateau, provides critical insights into the Mesozoic geological evolution of northeastern Thailand. This study presents the first integrated petrographic and geochemical investigation of the formation within Khon Kaen [...] Read more.
The Nam Phong Formation, a key unit of the pre-Khorat Group in the western Khorat Plateau, provides critical insights into the Mesozoic geological evolution of northeastern Thailand. This study presents the first integrated petrographic and geochemical investigation of the formation within Khon Kaen Geopark to reconstruct its Late Triassic–Early Jurassic depositional settings, provenance, and paleoclimate. A detailed stratigraphic section and five supplementary sites reveal litharenite and lithic wacke sandstones, interbedded with red paleosols and polymictic conglomerates. Sedimentary structures—such as trough and planar cross-bedding, erosional surfaces, and mature paleosols—indicate deposition in a high-energy braided fluvial system under semi-arid to subhumid conditions with episodic subaerial exposure. Petrographic analysis identifies abundant quartz, feldspar, and volcanic lithic fragments. Geochemical data and REE patterns, including diagnostic negative Ce anomalies, provide compelling evidence for provenance from active continental margins and oxidizing weathering conditions. These findings point to a tectonically active syn-rift basin influenced by climatic variability. Strikingly, the Nam Phong Formation exhibits paleoenvironmental and sedimentological features comparable to the modern Ebro Basin in northeastern Spain, highlighting the relevance of uniformitarian principles in interpreting ancient continental depositional systems. Full article
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18 pages, 12540 KB  
Article
SS-LIO: Robust Tightly Coupled Solid-State LiDAR–Inertial Odometry for Indoor Degraded Environments
by Yongle Zou, Peipei Meng, Jianqiang Xiong and Xinglin Wan
Electronics 2025, 14(15), 2951; https://doi.org/10.3390/electronics14152951 - 24 Jul 2025
Viewed by 714
Abstract
Solid-state LiDAR systems are widely recognized for their high reliability, low cost, and lightweight design, but they encounter significant challenges in SLAM tasks due to their limited field of view and uneven horizontal scanning patterns, especially in indoor environments with geometric constraints. To [...] Read more.
Solid-state LiDAR systems are widely recognized for their high reliability, low cost, and lightweight design, but they encounter significant challenges in SLAM tasks due to their limited field of view and uneven horizontal scanning patterns, especially in indoor environments with geometric constraints. To address these challenges, this paper proposes SS-LIO, a precise, robust, and real-time LiDAR–Inertial odometry solution designed for solid-state LiDAR systems. SS-LIO uses uncertainty propagation in LiDAR point-cloud modeling and a tightly coupled iterative extended Kalman filter to fuse LiDAR feature points with IMU data for reliable localization. It also employs voxels to encapsulate planar features for accurate map construction. Experimental results from open-source datasets and self-collected data demonstrate that SS-LIO achieves superior accuracy and robustness compared to state-of-the-art methods, with an end-to-end drift of only 0.2 m in indoor degraded scenarios. The detailed and accurate point-cloud maps generated by SS-LIO reflect the smoothness and precision of trajectory estimation, with significantly reduced drift and deviation. These outcomes highlight the effectiveness of SS-LIO in addressing the SLAM challenges posed by solid-state LiDAR systems and its capability to produce reliable maps in complex indoor settings. Full article
(This article belongs to the Special Issue Advancements in Robotics: Perception, Manipulation, and Interaction)
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32 pages, 8202 KB  
Article
A Machine Learning-Based Method for Lithology Identification of Outcrops Using TLS-Derived Spectral and Geometric Features
by Yanlin Shao, Peijin Li, Ran Jing, Yaxiong Shao, Lang Liu, Kunpeng Zhao, Binqing Gan, Xiaolei Duan and Longfan Li
Remote Sens. 2025, 17(14), 2434; https://doi.org/10.3390/rs17142434 - 14 Jul 2025
Viewed by 538
Abstract
Lithological identification of outcrops in complex geological settings plays a crucial role in hydrocarbon exploration and geological modeling. To address the limitations of traditional field surveys, such as low efficiency and high risk, we proposed an intelligent lithology recognition method, SG-RFGeo, for terrestrial [...] Read more.
Lithological identification of outcrops in complex geological settings plays a crucial role in hydrocarbon exploration and geological modeling. To address the limitations of traditional field surveys, such as low efficiency and high risk, we proposed an intelligent lithology recognition method, SG-RFGeo, for terrestrial laser scanning (TLS) outcrop point clouds, which integrates spectral and geometric features. The workflow involves several key steps. First, lithological recognition units are created through regular grid segmentation. From these units, spectral reflectance statistics (e.g., mean, standard deviation, kurtosis, and other related metrics), and geometric morphological features (e.g., surface variation rate, curvature, planarity, among others) are extracted. Next, a double-layer random forest model is employed for lithology identification. In the shallow layer, the Gini index is used to select relevant features for a coarse classification of vegetation, conglomerate, and mud–sandstone. The deep-layer module applies an optimized feature set to further classify thinly interbedded sandstone and mudstone. Geological prior knowledge, such as stratigraphic attitudes, is incorporated to spatially constrain and post-process the classification results, enhancing their geological plausibility. The method was tested on a TLS dataset from the Yueyawan outcrop of the Qingshuihe Formation, located on the southern margin of the Junggar Basin in China. Results demonstrate that the integration of spectral and geometric features significantly improves classification performance, with the Macro F1-score increasing from 0.65 (with single-feature input) to 0.82. Further, post-processing with stratigraphic constraints boosts the overall classification accuracy to 93%, outperforming SVM (59.2%), XGBoost (67.8%), and PointNet (75.3%). These findings demonstrate that integrating multi-source features and geological prior constraints effectively addresses the challenges of lithological identification in complex outcrops, providing a novel approach for high-precision geological modeling and exploration. Full article
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21 pages, 8652 KB  
Article
Unbiased Finite Element Mesh Delaunay Constrained Triangulation Applied to 2D Images with High Morphological Complexity Using Mathematical Morphology Tools Part 1: Binary Images
by Franck N’Guyen, Toufik Kanit and Abdellatif Imad
Computation 2025, 13(2), 52; https://doi.org/10.3390/computation13020052 - 13 Feb 2025
Viewed by 741
Abstract
We propose a method for establishing a Constrained Delaunay Triangulation CDT applied to 2D binary images of high morphological complexity. A prerequisite for CDT is the unbiased definition of the Planar Straight-Line Graph PSLG, which must respect the injective nature of Jordan’s Curve [...] Read more.
We propose a method for establishing a Constrained Delaunay Triangulation CDT applied to 2D binary images of high morphological complexity. A prerequisite for CDT is the unbiased definition of the Planar Straight-Line Graph PSLG, which must respect the injective nature of Jordan’s Curve whatever the topology of the image objects. Mathematical morphology provides tools for extracting the image contour, on which points will be judiciously placed at particular points to construct the vector path of the PSLG. Finally, these tools will enable us to implement a judicious pointing process in the image to guarantee the relative equivalence of triangles. The deterministic and rigorous procedure detailed in this article will be generalized in a second article, Part 2, to the case of labeled images for which the definition of the PSLG is more complex to define, since the contour of objects in the image is defined by the set of contours of adjacent objects. Full article
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22 pages, 5963 KB  
Article
A Light Field Depth Estimation Algorithm Considering Blur Features and Prior Knowledge of Planar Geometric Structures
by Shilong Zhang, Zhendong Liu, Xiaoli Liu, Dongyang Wang, Jie Yin, Jianlong Zhang, Chuan Du and Baocheng Yang
Appl. Sci. 2025, 15(3), 1447; https://doi.org/10.3390/app15031447 - 31 Jan 2025
Viewed by 1130
Abstract
Light field camera depth estimation is a core technology for high-precision three-dimensional reconstruction and realistic scene reproduction. We propose a depth estimation algorithm that fuses blurry features and planar geometric structure priors, aimed at overcoming the limitations of traditional methods in neighborhood selection [...] Read more.
Light field camera depth estimation is a core technology for high-precision three-dimensional reconstruction and realistic scene reproduction. We propose a depth estimation algorithm that fuses blurry features and planar geometric structure priors, aimed at overcoming the limitations of traditional methods in neighborhood selection and mismatching in weak texture regions. First, by constructing a multi-constraint adaptive neighborhood microimage set, the microimages with the lowest blur degree are selected to calculate matching costs, and sparse feature correspondence relationships are used to propagate depth information. Second, planar prior knowledge is introduced to optimize pixel matching costs in weak texture regions, and weights are dynamically adjusted and pixel matching costs are updated during the iterative propagation process within microimages based on matching window completeness. Then, potential mismatched points are eliminated using epipolar geometric relationships. Finally, experiments were conducted using public and real-world datasets for verification and analysis. Compared with famous depth estimation algorithms, such as Zeller and BLADE, the Our method demonstrates superior performance in quantitative depth estimation metrics, scene reconstruction completeness, object edge clarity, and depth scene coverage, providing richer and more accurate depth information. Full article
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20 pages, 7483 KB  
Article
An Enhanced LiDAR-Based SLAM Framework: Improving NDT Odometry with Efficient Feature Extraction and Loop Closure Detection
by Yan Ren, Zhendong Shen, Wanquan Liu and Xinyu Chen
Processes 2025, 13(1), 272; https://doi.org/10.3390/pr13010272 - 19 Jan 2025
Cited by 1 | Viewed by 2140
Abstract
Simultaneous localization and mapping (SLAM) is crucial for autonomous driving, drone navigation, and robot localization, relying on efficient point cloud registration and loop closure detection. Traditional Normal Distributions Transform (NDT) odometry frameworks provide robust solutions but struggle with real-time performance due to the [...] Read more.
Simultaneous localization and mapping (SLAM) is crucial for autonomous driving, drone navigation, and robot localization, relying on efficient point cloud registration and loop closure detection. Traditional Normal Distributions Transform (NDT) odometry frameworks provide robust solutions but struggle with real-time performance due to the high computational complexity of processing large-scale point clouds. This paper introduces an improved NDT-based LiDAR odometry framework to address these challenges. The proposed method enhances computational efficiency and registration accuracy by introducing a unified feature point cloud framework that integrates planar and edge features, enabling more accurate and efficient inter-frame matching. To further improve loop closure detection, a parallel hybrid approach combining Radius Search and Scan Context is developed, which significantly enhances robustness and accuracy. Additionally, feature-based point cloud registration is seamlessly integrated with full cloud mapping in global optimization, ensuring high-precision pose estimation and detailed environmental reconstruction. Experiments on both public datasets and real-world environments validate the effectiveness of the proposed framework. Compared with traditional NDT, our method achieves trajectory estimation accuracy increases of 35.59% and over 35%, respectively, with and without loop detection. The average registration time is reduced by 66.7%, memory usage is decreased by 23.16%, and CPU usage drops by 19.25%. These results surpass those of existing SLAM systems, such as LOAM. The proposed method demonstrates superior robustness, enabling reliable pose estimation and map construction in dynamic, complex settings. Full article
(This article belongs to the Section Manufacturing Processes and Systems)
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8 pages, 7391 KB  
Proceeding Paper
Comparative Analysis of LiDAR Inertial Odometry Algorithms in Blueberry Crops
by Ricardo Huaman, Clayder Gonzalez and Sixto Prado
Eng. Proc. 2025, 83(1), 9; https://doi.org/10.3390/engproc2025083009 - 9 Jan 2025
Viewed by 1888
Abstract
In recent years, LiDAR Odometry (LO) and LiDAR Inertial Odometry (LIO) algorithms for robot localization have considerably improved, with significant advancements demonstrated in various benchmarks. However, their performance in agricultural environments remains underexplored. This study addresses this gap by evaluating five state-of-the-art LO [...] Read more.
In recent years, LiDAR Odometry (LO) and LiDAR Inertial Odometry (LIO) algorithms for robot localization have considerably improved, with significant advancements demonstrated in various benchmarks. However, their performance in agricultural environments remains underexplored. This study addresses this gap by evaluating five state-of-the-art LO and LIO algorithms—LeGO-LOAM, DLO, DLIO, FAST-LIO2, and Point-LIO—in a blueberry farm setting. Using an Ouster OS1-32 LiDAR mounted on a four-wheeled mobile robot, the algorithms were evaluated using the translational error metric across four distinct sequences. DLIO showed the highest accuracy across all sequences, with a minimal error of 0.126 m over a 230 m path, while FAST-LIO2 achieved its lowest translational error of 0.606 m on a U-shaped path. LeGO-LOAM, however, struggled due to the environment’s lack of linear and planar features. The results underscore the effectiveness and potential limitations of these algorithms in agricultural environments, offering insights into future improvements and adaptations. Full article
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20 pages, 4895 KB  
Article
A Fast Two-Dimensional Direction-of-Arrival Estimator Using Array Manifold Matrix Learning
by Jieyi Lu, Long Yang, Yixin Yang and Lu Wang
Remote Sens. 2024, 16(24), 4654; https://doi.org/10.3390/rs16244654 - 12 Dec 2024
Viewed by 995
Abstract
Sparsity-based methods for two-dimensional (2D) direction-of-arrival (DOA) estimation often suffer from high computational complexity due to the large array manifold dictionaries. This paper proposes a fast 2D DOA estimator using array manifold matrix learning, where source-associated grid points are progressively selected from the [...] Read more.
Sparsity-based methods for two-dimensional (2D) direction-of-arrival (DOA) estimation often suffer from high computational complexity due to the large array manifold dictionaries. This paper proposes a fast 2D DOA estimator using array manifold matrix learning, where source-associated grid points are progressively selected from the set of predefined angular grids based on marginal likelihood maximization in the sparse Bayesian learning framework. This grid selection reduces the size of the manifold dictionary matrix, avoiding large-scale matrix inversion and resulting in reduced complexity. To overcome grid mismatch errors, grid optimization is established based on the marginal likelihood, with a dichotomizing-based solver provided that is applicable to arbitrary planar arrays. For uniform rectangular arrays, we present a 2D zoom fast Fourier transform as an alternative to the dichotomizing-based solver by transforming the manifold vector in a specific form, thus accelerating the computation without compromising accuracy. Simulation results verify the superior performance of the proposed methods in terms of estimation accuracy, computational efficiency, and angle resolution compared to state-of-the-art methods for 2D DOA estimation. Full article
(This article belongs to the Special Issue Ocean Remote Sensing Based on Radar, Sonar and Optical Techniques)
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18 pages, 11355 KB  
Article
Denoising Phase-Unwrapped Images in Laser Imaging via Statistical Analysis and DnCNN
by Yibo Xie, Jin Cheng, Shun Zhou, Qing Fan, Yue Jia, Jingjin Xiao and Weiguo Liu
Micromachines 2024, 15(11), 1372; https://doi.org/10.3390/mi15111372 - 14 Nov 2024
Viewed by 1178
Abstract
Three-dimensional imaging plays a crucial role at the micro-scale in fields such as precision manufacturing and materials science. However, image noise significantly impacts the accuracy of point cloud reconstruction, making image denoising techniques a widely discussed topic. Statistical analysis of laser imaging noise [...] Read more.
Three-dimensional imaging plays a crucial role at the micro-scale in fields such as precision manufacturing and materials science. However, image noise significantly impacts the accuracy of point cloud reconstruction, making image denoising techniques a widely discussed topic. Statistical analysis of laser imaging noise has led to the conclusion that logarithmically transformed noise follows a Gumbel distribution. A corresponding neural network training set was developed to address the challenges of difficult data collection and the scarcity of phase-unwrapped image datasets. Building on this foundation, a phase-unwrapped image denoising method based on the Denoising Convolutional Neural Network (DnCNN) is proposed. This method aims to achieve three-dimensional filtering by performing two-dimensional image denoising. Experimental results show a significant reduction in the Cloud-to-Mesh Distance (C2M) statistics of the corresponding point clouds before and after planar filtering. Specifically, the statistic at 97.5% of the 2σ principle decreases from 0.8782 mm to 0.3384 mm, highlighting the effectiveness of the filtering algorithm in improving the planar fit. Moreover, the DnCNN method exhibits exceptional denoising performance when applied to real-world target data, such as plaster statues with complex depth variations and PCBs made from different materials, thereby enhancing accuracy and reliability in point cloud reconstruction. This study provides valuable insights into phase-unwrapped image noise suppression in laser imaging, particularly in micro-scale applications where precision is critical. Full article
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21 pages, 10278 KB  
Article
Three-Dimensional Reconstruction of Zebra Crossings in Vehicle-Mounted LiDAR Point Clouds
by Zhenfeng Zhao, Shu Gan, Bo Xiao, Xinpeng Wang and Chong Liu
Remote Sens. 2024, 16(19), 3722; https://doi.org/10.3390/rs16193722 - 7 Oct 2024
Cited by 5 | Viewed by 2367
Abstract
In the production of high-definition maps, it is necessary to achieve the three-dimensional instantiation of road furniture that is difficult to depict on traditional maps. The development of mobile laser measurement technology provides a new means for acquiring road furniture data. To address [...] Read more.
In the production of high-definition maps, it is necessary to achieve the three-dimensional instantiation of road furniture that is difficult to depict on traditional maps. The development of mobile laser measurement technology provides a new means for acquiring road furniture data. To address the issue of traffic marking extraction accuracy in practical production, which is affected by degradation, occlusion, and non-standard variations, this paper proposes a 3D reconstruction method based on energy functions and template matching, using zebra crossings in vehicle-mounted LiDAR point clouds as an example. First, regions of interest (RoIs) containing zebra crossings are obtained through manual selection. Candidate point sets are then obtained at fixed distances, and their neighborhood intensity features are calculated to determine the number of zebra stripes using non-maximum suppression. Next, the slice intensity feature of each zebra stripe is calculated, followed by outlier filtering to determine the optimized length. Finally, a matching template is selected, and an energy function composed of the average intensity of the point cloud within the template, the intensity information entropy, and the intensity gradient at the template boundary is constructed. The 3D reconstruction result is obtained by solving the energy function, performing mode statistics, and normalization. This method enables the complete 3D reconstruction of zebra stripes within the RoI, maintaining an average planar corner accuracy within 0.05 m and an elevation accuracy within 0.02 m. The matching and reconstruction time does not exceed 1 s, and it has been applied in practical production. Full article
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27 pages, 17955 KB  
Article
Characterization of Complex Rock Mass Discontinuities from LiDAR Point Clouds
by Yanan Liu, Weihua Hua, Qihao Chen and Xiuguo Liu
Remote Sens. 2024, 16(17), 3291; https://doi.org/10.3390/rs16173291 - 4 Sep 2024
Cited by 1 | Viewed by 2394
Abstract
The distribution and development of rock mass discontinuities in 3D space control the deformation and failure characteristics of the rock mass, which in turn affect the strength, permeability, and stability of rock masses. Therefore, it is essential to accurately and efficiently characterize these [...] Read more.
The distribution and development of rock mass discontinuities in 3D space control the deformation and failure characteristics of the rock mass, which in turn affect the strength, permeability, and stability of rock masses. Therefore, it is essential to accurately and efficiently characterize these discontinuities. Light Detection and Ranging (LiDAR) now allows for fast and precise 3D data collection, which supports the creation of new methods for characterizing rock mass discontinuities. However, uneven density distribution and local surface undulations can limit the accuracy of discontinuity characterization. To address this, we propose a method for characterizing complex rock mass discontinuities based on laser point cloud data. This method is capable of processing datasets with varying densities and can reduce over-segmentation in non-planar areas. The suggested approach involves a five-stage process that includes: (1) adaptive resampling of point cloud data based on density comparison; (2) normal vector calculation using Principal Component Analysis (PCA); (3) identifying non-planar areas using a watershed-like algorithm, and determine the main discontinuity sets using Multi-threshold Mean Shift (MTMS); (4) identify single discontinuity clusters using Density-Based Spatial Clustering of Applications with Noise (DBSCAN); (5) fitting discontinuity planes with Random Sample Consensus (RANSAC) and determining discontinuity orientations using analytic geometry. This method was applied to three rock slope datasets and compared with previous research results and manual measurement results. The results indicate that this method can effectively reduce over-segmentation and the characterization results have high accuracy. Full article
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25 pages, 577 KB  
Article
Invariant Sets, Global Dynamics, and the Neimark–Sacker Bifurcation in the Evolutionary Ricker Model
by Rafael Luís and Brian Ryals
Symmetry 2024, 16(9), 1139; https://doi.org/10.3390/sym16091139 - 2 Sep 2024
Cited by 2 | Viewed by 1277
Abstract
In this paper, we study the local, global, and bifurcation properties of a planar nonlinear asymmetric discrete model of Ricker type that is derived from a Darwinian evolution strategy based on evolutionary game theory. We make a change of variables to both reduce [...] Read more.
In this paper, we study the local, global, and bifurcation properties of a planar nonlinear asymmetric discrete model of Ricker type that is derived from a Darwinian evolution strategy based on evolutionary game theory. We make a change of variables to both reduce the number of parameters as well as bring symmetry to the isoclines of the mapping. With this new model, we demonstrate the existence of a forward invariant and globally attracting set where all the dynamics occur. In this set, the model possesses two symmetric fixed points: the origin, which is always a saddle fixed point, and an interior fixed point that may be globally asymptotically stable. Moreover, we observe the presence of a supercritical Neimark–Sacker bifurcation, a phenomenon that is not present in the original non-evolutionary model. Full article
(This article belongs to the Special Issue Symmetry in Mathematical Models)
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14 pages, 279 KB  
Article
An Improvement of the Upper Bound for the Number of Halving Lines of Planar Sets
by Estrella Alonso, Mariló López and Javier Rodrigo
Symmetry 2024, 16(7), 936; https://doi.org/10.3390/sym16070936 - 22 Jul 2024
Cited by 1 | Viewed by 1994
Abstract
In this paper, we provide improvements in the additive constant of the current best asymptotic upper bound for the maximum number of halving lines for planar sets of n points, where n is an even number. We also improve this current best upper [...] Read more.
In this paper, we provide improvements in the additive constant of the current best asymptotic upper bound for the maximum number of halving lines for planar sets of n points, where n is an even number. We also improve this current best upper bound for small values of n, namely, 106n336. To obtain this enhancements, we provide lower bounds for the sum of the squares of the degrees of the vertices of a graph related to the halving lines. Full article
(This article belongs to the Special Issue Symmetry in Nonlinear Partial Differential Equations and Rogue Waves)
2 pages, 129 KB  
Abstract
Development of a Fully Automated Microfluidic Electrochemical Sensor on the ESSENCE Platform for Rapid Detection of Single-Stranded DNA
by Niranjan Haridas Menon, Maryom Rahman and Sagnik Basuray
Proceedings 2024, 104(1), 17; https://doi.org/10.3390/proceedings2024104017 - 28 May 2024
Viewed by 732
Abstract
This study presents a fully automated microfluidic electrochemical sensor for the detection of single-stranded DNA (ssDNA) on the ESSENCE platform. The sensor utilizes functionalized single-walled carbon nanotubes (SWCNTs) with short ssDNA strands immobilized through EDC-NHS coupling, placed between non-planar interdigitated electrodes. The detection [...] Read more.
This study presents a fully automated microfluidic electrochemical sensor for the detection of single-stranded DNA (ssDNA) on the ESSENCE platform. The sensor utilizes functionalized single-walled carbon nanotubes (SWCNTs) with short ssDNA strands immobilized through EDC-NHS coupling, placed between non-planar interdigitated electrodes. The detection process involves sequential flow of a background electrolyte and redox probe through the microfluidic channel before introducing the target DNA solution. The same solution is then circulated to enhance selectivity by removing non-specifically bound targets. Electrochemical impedance signals are acquired after the initial and final flow steps, utilizing changes in impedance spectra to quantify target DNA concentration. To streamline complex flow steps and eliminate manual interventions, the system integrates a fully automated fluid control system with syringe pumps, valves, and pressure sensors. Electrochemical impedance spectroscopy (EIS) data is acquired using the Analog Discovery 2 USB oscilloscope, and LabVIEW automation ensures a seamless transition from sample introduction to data acquisition. The transducer material’s flow-through design enables efficient differentiation between different degrees of base pair mismatches, extending applicability to single nucleotide polymorphisms. The system exhibits high sensitivity, detecting single-stranded DNA at concentrations as low as 1 fM within a rapid 15-min detection time. Its compact design and automated data acquisition make it a promising candidate for point-of-care biomolecule sensing, including antigens and toxins. Future applications involve functionalizing SWCNTs with relevant antibodies to enhance the platform’s capabilities for detecting a diverse range of target molecules in clinical settings. Full article
(This article belongs to the Proceedings of The 4th International Electronic Conference on Biosensors)
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