Search Results (2,098)

Mucuna pruriens (L.) DC. (Fabaceae), commonly known as velvet bean, is an annual tropical legume widely distributed in India, Africa, and the Americas. It has a long history of use in traditional medicine for managing various health conditions. It is renowned for its anti-inflammatory, antimicrobial, aphrodisiac, and anti-Parkinson effects. The entire plant is considered health-promoting, particularly the seeds. They have been used for their neuroprotective, fertility-enhancing, and antioxidant effects. This review aims to compile all available information regarding the chemical composition of all parts of this medicinal plant. For this purpose, the complete databases of Google Scholar, Scopus, PubMed, and Web of Science available to date were utilized. All studies reported the presence of a diverse range of secondary metabolites, including phenolic compounds, such as phenolic acids, flavonoids, and tannins, as well as saponins and alkaloids. Most studies concentrated on the chemical characterization of the seeds, whereas the leaves, roots, and pods have received comparatively limited scientific attention. The seeds of M. pruriens are renowned for their high concentration of L-3,4-dihydroxyphenylalanine (L-DOPA), a metabolic precursor of dopamine. A large proportion of these studies originated from countries where M. pruriens naturally occurs. Few studies have been conducted on the chemical composition of velvet bean outside these regions. Despite the existing information on the chemical composition of M. pruriens. (seeds, leaves, and roots), further research beyond its natural habitat is required to gain a broader understanding of its chemical profile and pharmacological effects. Full article

Chrysanthemum indicum L. (C. indicum), a perennial herb widely distributed across East Asia, has long been utilised in traditional medicine and as a functional food ingredient. Contemporary research has revealed a chemically diverse phytochemical profile, dominated by flavonoids, phenolic acids, sesquiterpene lactones, essential oils, carotenoids, and polysaccharides, which collectively underpin its broad pharmacological potential. Experimental studies demonstrate that extracts and isolated constituents of C. indicum exert pronounced antioxidant, anti-inflammatory, antimicrobial, hepatoprotective, cardioprotective, and anticancer effects in vitro and in vivo, often through modulation of key molecular pathways such as NF-κB, NLRP3 inflammasomes, AMPK–SIRT1, and Nrf2 signalling. Emerging pharmacokinetic evidence indicates variable oral bioavailability and metabolic transformation of major bioactive compounds, highlighting formulation challenges that may influence therapeutic efficacy. Toxicological studies suggest a generally favourable safety profile at traditionally used doses, although long-term and clinical safety data remain limited. Regulatory positioning varies internationally, with applications spanning traditional herbal preparations, dietary supplements and functional foods. Despite promising preclinical findings, significant challenges persist, including chemical standardisation, bioavailability optimisation, mechanistic clarification and the paucity of well-designed clinical trials. This review critically synthesises current knowledge on the botany, phytochemistry, pharmacological activities, pharmacokinetics, safety considerations and regulatory landscape of C. indicum, identifying key research gaps and outlining future directions to support its evidence-based development as a therapeutic and dietary agent. Full article

This study investigated the effect of solvent polarity on extraction yield, phytochemical composition, antioxidant activity, and α-amylase inhibition of Elaeagnus angustifolia L. leaf extracts to evaluate their antidiabetic potential. Extraction yields varied with solvent polarity, with the hydroethanolic extract showing the highest (18.00%) and n-hexane the lowest (0.05%) yield. The n-butanol and ethyl acetate fractions contained the most phenolics (309.05 and 290.97 mg GAE/g), ethyl acetate was the richest in flavonoids (102.11 mg QE/g), and tannins were concentrated in dichloromethane (66.24 mg CE/g). HPLC revealed solvent-specific profiles: rutin and gallic acid dominated in n-butanol, quercetin in ethyl acetate, and 4-hydroxybenzoic and ferulic acids in dichloromethane, while chicoric acid appeared in hydroethanolic and n-hexane extracts. Antioxidant assays (DPPH, ABTS, and FRAP) showed strong activity in polar extracts, particularly hydroethanolic and ethyl acetate fractions. The n-hexane extract exhibited the highest α-amylase inhibition (IC₅₀ = 36.70 µg/mL), surpassing acarbose (IC₅₀ = 126.14 µg/mL), while other fractions were inactive (IC₅₀ > 400 µg/mL). Molecular docking highlighted rutin, chlorogenic acid, and chicoric acid as potential enzyme binders. These findings demonstrate the chemical diversity and significant bioactivities of E. angustifolia leaves, supporting their potential as natural antidiabetic agents. Full article

In recent years, the global use of dietary supplements has surged, largely due to their appeal as tools for improving well-being and preventing disease. While these products may offer clear advantages—such as addressing micronutrient shortages and enhancing physical or mental performance—they also carry significant risks, including toxicity, potential drug interactions, and limited clinical validation. This paper explores the current body of scientific literature on dietary supplements, offering a nuanced analysis of their advantages and drawbacks, particularly in general and military populations. Drawing upon peer-reviewed studies, regulatory documents, and expert guidelines, the review outlines key safety considerations and presents practical recommendations for evidence-based use. In addition to conventional formulations, attention is given to the emergence of nutritional gels and hydrogel-based delivery systems, which are increasingly investigated as strategies to improve portability, gastrointestinal tolerability, and bioavailability of bioactive compounds in high-demand civilian and occupational settings. These platforms illustrate a broader shift toward advanced supplement technologies and precision nutrition approaches. Full article

Background/Objectives: Commiphora gileadensis (Balm of Gilead) is an aromatic medicinal plant with a history of traditional use in ancient and Arabic medicine. It has been used traditionally to treat inflammation, infections, and wounds. Despite its long-standing cultural and economic importance, modern pharmacological validation requires a comprehensive synthesis of current scientific data. This review aims to provide a thorough comparative summary of the phytochemical composition and biological activities of its diverse products. Methods: An updated literature search was conducted using databases such as ScienceDirect, PubMed, Scopus, and Google Scholar, covering publications from approximately 2000 to 2025. The review included English-language peer-reviewed articles, books, and reports providing phytochemical analyses or biological evaluations. Data were manually extracted and categorized by plant parts (resin, leaves, bark, stems), major constituents, and specific pharmacological activities. Results: The review identified ten diverse chemical groups, mainly terpenoids (mono-, sesqui-, di-, and triterpenes) and flavonoids. Other remarkable classes included phenolic acids, phytosterols, lignans, coumarins, and fatty acids. However, the essential oil chemical profile is highly variable, influenced by geographical origin and preparation technique. Pharmacological studies demonstrated a wide spectrum of bioactivities, in particular antioxidant, anti-inflammatory, antimicrobial, anticancer, antidiabetic, and wound-healing properties. Toxicological studies classified the plant as generally non-toxic; however, there is a notable lack of clinical and pharmacokinetic data. Conclusions:C. gileadensis possesses a rich and diverse secondary metabolite profile, validating its traditional ethnobotanical applications. Future research should prioritize pre-clinical and clinical trials to establish its safety, bioavailability, and metabolic fate for its successful integration into modern medicine. Full article

With rising global temperatures, biostimulants might be a promising tool to alleviate plant stress and support adaptation. The potential of fresh (FBP) and lyophilized (LBP) banana peel aqueous extracts as biostimulants for protecting broccoli from high temperature (HT) stress was analyzed. Spectrophotometric and statistical analyses revealed that BP affected broccoli phytochemistry in a temperature-dependent manner. Under room temperature (RT), FBP and LBP decreased glucosinolates (−15% and −25%, respectively). Conversely, FBP increased flavonols and proanthocyanidins (141% and 202%, respectively). Under RT, LBP decreased sugars in broccoli (−27%). FBP had stronger effects at HT than at RT, further boosting phenolics (70%), flavonoids (89%), tannins (31%), and hydroxycinnamic acids (64%), and antioxidant capacity (FRAP) (10%). LBP also increased flavonoids (39%), flavonols (95%), and hydroxycinnamic acids (45%) under HT. Both FBP and LBP increased glucosinolates (47% and 46%, respectively) in HT-grown broccoli. HT significantly affected glucosinolates, decreased them in control plants, and increased them in BP-treated plants. All HT-grown plants had higher soluble sugars and lower hydrogen peroxide than RT-grown plants. Principal component analysis confirmed greater biochemical diversity under HT. Temperature–BP interaction significantly affected flavonoids and glucosinolates, highlighting the central role of environmental temperature in determining biostimulant outcomes. These findings suggest that global warming may markedly alter biostimulant efficacy and should be considered in their development. Full article

Ziziphus lotus (L.) Lam., an extremophyte shrub native to the Mediterranean basin, yields underexplored fruits as a source of therapeutic agents. This study combined in vitro and in silico approaches to evaluate the antioxidant potential of Z. lotus fruits and predict their potential to inhibit xanthine oxidase (XO), a key enzyme in reactive oxygen species generation and oxidative stress-related pathologies. The ethyl acetate extract from the hydroalcoholic macerate was enriched in total phenolics (281.33 ± 1.5 μg GAE/mg) and flavonoids (127.26 ± 5.89 μg RE/mg) and displayed remarkable effects against the ABTS^•+ radical cation (IC₅₀ = 18.49 ± 1.47 μg/mL) and phenanthroline reducing power (A_0.5 = 8.38 ± 0.69 μg/mL), together with measurable xanthine oxidase inhibition (IC₅₀ = 170.4 ± 5.90 μg/mL). The compounds tentatively identified by full-scan UHPLC-QtoF-HRMS were docked against XO (PDB ID: 3NVY), with phytosphingosine (−8.5 kcal/mol) and rutin (−8.3 kcal/mol) exhibiting the strongest binding affinities, forming favorable predicted interactions with critical catalytic residues, followed by 6‴-feruloylspinosin, 3′,5′-di-C-β-glucopyranosylphloretin and hexadecasphinganine (ranging from −7.8 to −7.6 kcal/mol). Predictive structure–activity relationships were also observed. These results provide insights into the antioxidant potential of Z. lotus phytochemicals and highlight the value of this extremophile plant as sustainable resource for phytotherapy and the management of oxidative stress-related diseases. Full article

Both riparian tree species diversity and genetic diversity can influence streams through leaf litter decomposition; however, these two sources of variation have not been compared directly. Here, we compare leaf litter decomposition for a physical mixture of two Populus species to the litter from an F₁ hybrid (a genetic mixture) of the same two species. Leaf litter was collected from a common garden for an F₁ hybrid between Japanese poplar (Populus maximowiczii A. Henry) and black cottonwood (Populus trichocarpa Torr. & A. Gray ex Hook.), as well as both parent species. Four litterbag treatments consisted of litter from the F₁ hybrid, an equal-part mixture treatment of litters from both parents, and litter from each parent in isolation. The hybrid litter had higher C:N content and lower % condensed tannins than either parent species, or the average of the two parents that represented the mixture. While the hybrid and mixture treatments both lost more mass than expected by day 42, the mixture lost relatively more mass than the hybrid and roughly as much as the faster-decomposing P. trichocarpa parent. The hybrid mimicked the mixture, and both supported higher aquatic macroinvertebrate richness and less litter mass remaining than expected based on parent species values, despite differences between the hybrid and mixture in initial phytochemistry. Full article

Background/Objectives: Cancer has been associated with significant morbidity and mortality worldwide, particularly related to chemotherapy resistance. Therefore, it is essential to investigate alternative sources of non-toxic antitumor compounds. The cactus Lophocereus marginatus is native to Mexico and is commonly used to treat gastrointestinal infections and diabetes in traditional medicine. Methods: The in vitro antitumor activity of L. marginatus extract fractions against murine L5178Y-R lymphoma cells was evaluated. The crude extract and its solvent-derived fractions were evaluated for cytotoxicity, selectivity, and hemolytic activity. Results: The crude extract exhibited an IC₅₀ of 9.09 μg/mL, demonstrating a high selectivity index (SI: 330.03), with no hemolytic activity observed at 1000 μg/mL. The LM-HP, LM-CP, and LM-MP partitions showed varying IC₅₀ values (6.74, 7.93, and 45.38 μg/mL, respectively) and selectivity indices of 445.1, 378.31, and 66.1, respectively. Only LM-HP induced hemolysis at 200 μg/mL. The most promising fraction, CP-F8, exhibited an IC₅₀ of 11.2 μg/mL, high selectivity index (354.29), and antioxidant activity, without hemolytic effects. Phytochemical analysis of CP-F8 identified phenolic compounds, triterpenes, and sterols, which are known for their anti-cancer and anti-inflammatory properties. In vivo tests showed no significant liver damage or changes in body weight, indicating the safety of CP-F8. Conclusions: These results suggest that CP-F8 is a promising antitumor candidate with selective cytotoxicity and minimal toxicity to normal cells. Full article

Rosa roxburghii Tratt., commonly known as Cili, is an emerging functional fruit native to southwestern China, characterized by extraordinarily high vitamin C content, robust superoxide dismutase activity, and a rich diversity of bioactive compounds. This review summarizes recent advances in its phytochemistry, molecular bioactivity, and omics-driven applications, with a focus on integrating evidence from genomic, transcriptomic, and metabolomic analyses. Multi-omics analyses reveal the coordinated regulation of ascorbate and secondary metabolite accumulation via key pathways and transcription factors. Mechanistically, bioactive compounds from Cili comprising ascorbate derivatives, polyphenols, flavonoids, polysaccharides, triterpenoids, and sterols, act synergistically. They also exhibit antioxidant, anti-inflammatory, gastrointestinal, hepatoprotective, cardiovascular protective, anti-obesity, anti-diabetic, metabolic regulatory, anti-cancer, and neuroprotective effects by modulating core metabolic and stress-response signaling pathways. Recent advances in functional food processing have further elevated its nutritional and industrial value, highlighting its potential as a sustainable nutraceutical resource. Full article

Helichrysum Mill. is one of the largest genera in the Asteraceae family, and in Madagascar, a plant paradise with ca 14,000 species, 111 Helichrysum species are known, 110 of which are endemic. In this work, the essential oil of endemic Helichrysum manopappoides Humbert, obtained by steam distillation, was investigated chemically and biologically. The spectrometric data obtained by GC-MS (Gas Chromatography–Mass Spectrometry) analysis highlighted the presence of three major compounds, such as eucalyptol (7.38 ± 0.36%), α-humulene (14.75 ± 0.79%), and β-caryophyllene (19.78 ± 0.89%), which were also structurally confirmed by NMR (Nuclear Magnetic Resonance) spectroscopic investigation. Biological results showed potential antimicrobial, antioxidant, and antibiofilm effects of both the H manopappoides essential oil and the main components identified by GC-MS, enhancing an interesting approach for intestinal infections, being active against Escherichia coli, Listeria monocytogenes, Shigella sonnei, and Salmonella enterica ser. typhimurium strains. Full article

Diabetes mellitus is a chronic disease requiring multifunctional natural agents. Arctium lappa is traditionally used in Eastern and European medicine to address metabolic disorders. This comprehensive narrative review, conducted between 2000 and 2025 using international databases (Scopus, PubMed, Web of Science Core Collection, and Google Scholar), evaluates the species through its ethnomedicine, phytochemistry, preclinical evidence, and safety. The available evidence suggests that A. lappa exerts antidiabetic effects via multi-layered mechanisms, including AMPK activation, insulin signaling modulation, and increased GLUT4 translocation. Key bioactives (arctigenin, arctiin, and inulin) collectively improve insulin sensitivity and lipid metabolism. However, preclinical studies confirm these effects in animal models, while limited clinical data in non-diabetic cohorts focus on systemic inflammation. This highlights a significant gap in randomized controlled trials targeting glycemic control in diabetic populations. In this context, while A. lappa shows promise as a potential metabolic regulator; this evidence is currently derived primarily from in vitro and animal models. Systematic clinical trials are urgently required to establish glycemic efficacy in humans, validate its therapeutic potential, and determine the optimal dosage and safety profile. This review evaluates the multi-targeted biological potential of A. lappa to guide future research and evidence-based application. Full article

Human African Trypanosomiasis (HAT) and Malaria are serious infectious diseases endemic in tropical regions, caused by protozoan parasites, and necessitating an urgent development of new antiprotozoal drugs. As part of our ongoing search for new antiprotozoal steroidal alkaloids from plants, we investigated the methanolic stem bark extract of Holarrhena pubescens (Apocynaceae). H. pubescens is a tropical tree that some Kenyan coastal communities have long used to treat various ailments, including fever and stomach pain. The crude extract, alkaloid fraction, and 16 subfractions acquired through centrifugal partition chromatography (CPC) displayed promising in vitro antiprotozoal activity against Trypanosoma brucei rhodesiense (Tbr) and Plasmodium falciparum (Pf). Partial least squares (PLS) regression modeling of UHPLC/+ESI QqTOF-MS data and the antiprotozoal activity data of the crude extract and its fractions was performed to predict compounds that may be responsible for the observed antiplasmodial activity. Chromatographic separation of the alkaloid fraction afforded one new steroidal alkaloid (5), along with 18 known compounds (1, 2, 4, 6–20), and one artifact (3) that was presumably formed during the acid–base extraction process. The structural characterization of the isolated compounds was accomplished using UHPLC/+ESI-QqTOF-MS/MS and NMR spectroscopy. The isolated compounds were tested for their in vitro antiprotozoal properties against the two aforementioned pathogens, as well as for their cytotoxicity against mammalian cells (L6 cell line). Compounds 2 and 16 (IC₅₀ = 0.2 μmol/L) demonstrated the highest antitrypanosomal activity, with compound 2 showing the highest selectivity (SI = 127). The new compound 5 exhibited the strongest antiplasmodial activity and selectivity against Pf (IC₅₀ = 0.7 μmol/L, SI = 43). Our findings provide further promising antiprotozoal leads for HAT and Malaria. Full article

Melissa officinalis L. (lemon balm) is a Lamiaceae species widely used in traditional and contemporary herbal practice, yet its reported bioactivities are strongly preparation-dependent, reflecting variability between polyphenol-rich extracts and volatile essential-oil fractions. This integrative review links phytochemistry with recent preclinical findings and available clinical evidence. Across model systems, lemon balm most consistently shows antioxidant and anti-inflammatory signatures, with additional domain-specific signals reported in neurobehavioral, cardiometabolic, gastrointestinal, and dermatological models; however, comparability is limited by heterogeneous plant parts, extraction procedures, and chemical standardization. Preclinical findings were organized by biological domain, whereas clinically, the most consistent signals are observed for symptom-oriented endpoints, particularly anxiety/stress and sleep-related outcomes reported in controlled trials, including aromatherapy studies, while evidence for other indications remains mixed or insufficiently confirmed. Overall, the evidence supports continued development of chemically characterized, standardized preparations and mechanism-informed trials with harmonized outcomes and robust safety reporting to improve translational interpretability. Full article

The COVID-19 pandemic necessitated rigorous infection control protocols across hospital environments, particularly in nutrition services. UV-C light emerged as a viable option due to its shortest wavelength, conferring the greatest capacity for nucleic acid (DNA/RNA) penetration in both microorganisms and human cells. We aim to develop and validate an alternative method for decontaminating food carts using UV-C light. A prototype box was created to cover the meal transport carts, with UV-C lamps strategically positioned for irradiation and decontamination. To validate this technology, four different hygiene protocols were compared: Group 1, positive control, with no sanitization procedure; Group 2, cleaned using the standard protocol of the University Hospital of UFS (HU/UFS) with 70% ethanol solution; Group 3, negative control, cleaned using the standard HU/UFS protocol with 70% ethanol and neutral detergent; and Group 4, exposed to UV-C light irradiation for 10 min after food transport. Microbiological samples were collected from 15 distinct points on the carts. Samples were collected using sterile swabs moistened with 0.85% saline solution and incubated in BHI broth at 37 °C for 24 h for presence/absence testing. Positive samples were plated on selective media (CLED, SS, MacConkey and Blood agar), incubated at 37 °C for 24–48 h, and evaluated for colony-forming unit (CFU) count. A statistically significant association was found between the hygiene method and the presence of microbial contamination (p < 0.001), based on both bacterial plate counts and BHI broth growth. Carts sanitized with 70% alcohol showed a lower probability of contamination compared to the control group (alcohol + detergent), while unsanitized carts showed a higher risk. UV-C light treatment resulted in complete absence of bacterial growth. UV-C disinfection demonstrated superior effectiveness in eliminating microorganisms, indicating a promising alternative to traditional methods. Full article