Search Results (94)

In the digital communication ecosystem, micro-influencers have influenced public response during crises, especially in complex geopolitical contexts. This paper introduces the micro-influencer impact model (MIIM), a framework for analyzing the impact of micro-influencers on crisis communication. The MIIM integrates four components (micro-influencer characteristics, message framing and delivery, audience factors, and crisis context) offering a comprehensive approach to understanding micro-influencer dynamics during crises. Cross-conflict analysis spanning Ukraine–Russia, Sudan–Ethiopia, Armenia–Azerbaijan, Myanmar, Syria, and India–Pakistan tensions demonstrates the MIIM’s broad applicability across diverse geopolitical crises, showing how factors like perceived authenticity, niche expertise, narrative personalization, and audience digital literacy consistently shape public opinion and crisis response. The MIIM synthesizes crisis communication theories, social influence models, and digital media research, providing a sophisticated framework for studying the dissemination of information and public engagement during crises. The paper proposes theoretically grounded propositions on the impact of micro-influencers, encompassing perceived authenticity, narrative framing, and influence over time, thereby laying the groundwork for future empirical research. Implications for communication scholars, crisis managers, policymakers, and social media platforms are discussed, emphasizing the MIIM’s relevance to theory and practice in crisis communication. Full article

Global biodiversity losses continue despite intensive conservation efforts. Many mammal species are understudied due to their specialized ecological niches. One such species is the Philippine colugo (Cynocephalus volans), a nocturnal endemic species in the Philippines. In this study, we utilized Local Ecological Knowledge (LEK) to obtain baseline information on species knowledge, attitudes, population status, and threats. Between June and September 2023, we interviewed 471 residents across all villages in Anda, Bohol. The majority of local people recognized the species and had witnessed it in Anda, occasionally near households. Residents have limited knowledge of colugo diet and distribution, which they get primarily through word-of-mouth and personal experience. The species is perceived as neutral, but the willingness to conserve it is high. Although the Philippine colugo population seems to be present in several villages in Anda, one-fourth of the respondents believe it is declining. We identified hunting for consumption as the main threat to the colugo population in Anda, which, together with other threats, may corroborate this result. We recommend actively involving male farmers in colugo population monitoring and behavioral observations, as well as investigating the drivers and importance of colugo meat consumption among residents to design a proper conservation strategy. Full article

Alzheimer’s disease (AD) is characterized by progressive cognitive decline strongly associated with impaired adult hippocampal neurogenesis (AHN). Mounting evidence suggests that this impairment results from both the intrinsic dysfunction of neural stem cells (NSCs)—such as transcriptional alterations in quiescent states—and extrinsic niche disruptions, including the dysregulation of the Reelin signaling pathway and heightened neuroinflammation. Notably, AHN deficits may precede classical amyloid-β and Tau pathology, supporting their potential as early biomarkers of disease progression. In this review, we synthesize recent advances in therapeutic strategies aimed at restoring AHN, encompassing pharmacological agents, natural products, and non-pharmacological interventions such as environmental enrichment and dietary modulation. Emerging approaches—including BDNF-targeted nanocarriers, NSC-derived extracellular vesicles, and multimodal lifestyle interventions—highlight the translational promise of enhancing neurogenesis in models of familial AD. We further propose the Neurogenesis Impairment Index (NII)—a novel composite metric that quantifies hippocampal neurogenic capacity relative to amyloid burden, while adjusting for demographic and cognitive variables. By integrating neurogenic potential, cognitive performance, and pathological load, NII provides a framework for stratifying disease severity and guiding personalized therapeutic approaches. Despite ongoing challenges—such as interspecies differences in neurogenesis rates and the limitations of stem cell-based therapies—this integrative perspective offers a promising avenue to bridge mechanistic insights with clinical innovation in the development of next-generation AD treatments. Full article

This study explores the dynamics of motorcycle tourism in rural areas and its potential contribution to sustainable and regenerative development. A bibliometric analysis of management-related publications was conducted using the Web of Science Core Collection, focusing on topics such as tourism, motivation, rurality, and motorcycling. VOSviewer software (version 1.6.20) was employed to support this analysis. Based on the literature, a theoretical framework was developed, leading to four research hypotheses that aimed to empirically examine the relationships between cultural motivation, community interaction, type of accommodation, event location, and tourist behaviour. To test these hypotheses, structured questionnaires were distributed in person during rural motorcycling events in Portugal, yielding a valid sample of 233 respondents. The data were analysed using SPSS 28 software via statistical methods to reduce dimensionality and identify latent structures, chi-square tests, and logistic regression. The results confirmed all four hypotheses, highlighting the importance of cultural motivation for return intentions, community interaction for perceived authenticity, accommodation type for destination recommendations, and event location for overall satisfaction. The study also identifies gender-related differences and reinforces the value of immersive, co-created experiences in enhancing the competitiveness of rural destinations. This theoretical contribution supports the advancement of motorcycle tourism as a sustainable niche while offering practical guidance for inclusive and regenerative tourism planning. Full article

Recent advancements in spatial transcriptomics (ST) have revolutionized our understanding of the lung’s cellular organization and pathological alterations. By preserving the spatial distribution of gene expression, ST reveals localized immune niches, stromal–epithelial interactions, and disease-associated transcriptional “hotspots” that cannot be captured by conventional sequencing methods alone. In lung cancer, ST-based investigations have delineated distinct tumor microenvironments between tumor cores and invasive fronts, revealing prognostically significant gene signatures and identifying subpopulations with differential responses to immunotherapy and chemotherapy. Similarly, in chronic obstructive pulmonary disease, asthma, and idiopathic pulmonary fibrosis, ST has mapped the ecosystem, including immune cells, inflammatory mediators, and fibroblast subtypes, of discrete regions within diseased lung tissue, offering mechanistic insights into disease progression and tissue remodeling. In addition, a more recent ST study provides spatial information for where drugs act within tissues. This review highlights the emerging role of spatial transcriptomics in respiratory research, demonstrating its potential to refine disease classification, elucidate mechanisms of therapeutic resistance, and inform spatially guided personalized interventions in respiratory diseases. Full article

This paper examines strategies aimed at improving search procedures in multimodal, low-dimensional domains. Here, low-dimensional domains refers to a maximum of five dimensions. The present analysis assembles strategies to form an algorithm named S-EPSO, which, at its core, locates and maintains multiple optima without relying on external niching parameters, instead adapting this functionality internally. The first proposed strategy assigns socio-emotional personalities to the particles forming the swarm. The analysis also introduces a technique to help them visit secluded zones. It allocates the particles of the initial distribution to subdomains based on biased decisions. The biases reflect the subdomain’s potential to contain optima. This potential is established from a balanced combination of the jaggedness and the mean-average interval descriptors developed in the study. The study compares the performance of S-EPSO to that of state-of-the-art algorithms over seventeen functions of the CEC benchmark, and S-EPSO is revealed to be highly competitive. It outperformed the reference algorithms 14 times, whereas the best of the latter outperformed the other two 10 times out of 30 relevant evaluations. S-EPSO performed best with the most challenging 5D functions of the benchmark. These results clearly illustrate the potential of S-EPSO when it comes to dealing with practical engineering optimization problems limited to five dimensions. Full article

Neural stem cells (NSCs) are crucial components of the nervous system, primarily located in the subventricular zone (SVZ) and subgranular zone (SGZ). The SVZ neural stem cell niche (NSCN) is a specialized microenvironment where growth factors and extracellular matrix (ECM) components collaborate to regulate NSC self-renewal and differentiation. Despite its importance, our understanding of the SVZ remains incomplete due to the inherent challenges of animal research, particularly given the tissue’s dynamic nature. To address these limitations, we developed a proof-of-concept, dynamic, and tissue-specific 3D organotypic SVZ model to reduce reliance on animal models. This static 3D organotypic model integrates a region-specific decellularized ECM derived from the SVZ, mimicking the native NSCN and supporting mouse-derived ependymal cells (ECs), radial glial cells (RGCs), astrocytes, and NSCs. To further improve physiological relevance, we incorporated a dynamic microfluidic culture system (SVZonChip), replicating cerebrospinal fluid (CSF) flow as observed in vivo. The resulting SVZonChip platform, combining region-specific ECM proteins with dynamic culture conditions, provides a sustainable and reproducible tool to minimize animal model use. It holds significant promise for studying SVZ-related diseases, such as congenital hydrocephalus, stroke, and post-stroke neurogenesis, while advancing translational research and enabling personalized medicine protocols. Full article

Cancer remains a formidable global health challenge due to its complex pathophysiology and resistance to conventional treatments. In recent years, the convergence of nanotechnology and oncology has paved the way for innovative therapeutic platforms that address the limitations of traditional modalities. This review examines how nanoparticle (NP)-based strategies enhance the efficacy of chemotherapy, radiotherapy, phototherapy, immunotherapy, and gene therapy by enabling targeted delivery, controlled drug release, and tumor-specific accumulation via the enhanced permeability and retention (EPR) effect. We discuss the design and functionalization of various organic, inorganic, and hybrid NPs, highlighting their roles in improving pharmacokinetics, overcoming multidrug resistance, and modulating the tumor microenvironment. Particular emphasis is placed on dual and multimodal therapies, such as chemo-phototherapy, chemo-immunotherapy, and gene-radiotherapy, that leverage nanoparticle carriers to amplify synergistic effects, minimize systemic toxicity, and improve clinical outcomes. We also explore cutting-edge advances in gene editing and personalized nanomedicine, as well as emerging strategies to address biological barriers and immunosuppressive mechanisms in the tumor niche. Despite the undeniable promise of nanoparticle-based cancer therapies, challenges related to toxicity, scalable manufacturing, regulatory oversight, and long-term biocompatibility must be overcome before they can fully enter clinical practice. By synthesizing recent findings and identifying key opportunities for innovation, this review provides insight into how nanoscale platforms are propelling the next generation of precision oncology. Full article

In certain newly established or niche e-commerce platforms, user–item interactions are often exceedingly sparse due to limited user bases or specialized product lines, posing significant obstacles to accurate personalized recommendations. To address these challenges, this paper proposes an enhanced recommendation approach based on a latent factor model. By leveraging factorization to uncover the hidden features of users and items and incorporating both user behavioral data and item attribute information, a multi-dimensional latent semantic space is constructed to more effectively capture the underlying relationships between user preferences and item properties. The method involves data preprocessing, model construction, user and item vectorization, and semantic-similarity-based recommendation generation. For empirical validation, we employ a real-world dataset gathered from an e-commerce platform, comprising 4645 ratings from 3445 users across 277 items in nine distinct categories. Experimental results demonstrate that, compared with conventional collaborative filtering methods, this approach achieves superior precision and recall even in highly sparse settings, showing stronger resilience under low-density conditions. These findings offer objective and feasible insights for advancing personalized recommendation techniques in newly established or niche e-commerce platforms. Full article

The Ligurian Sea, located in the northwestern Mediterranean, is undergoing a dramatic shift in fish biodiversity due to climate change and species immigration. This study adopted a citizen science approach to provide new data on the current distribution of rare, thermophilic, and alien fish species in the Ligurian Sea. Observations were collected through social networks and personal acquaintances, involving fishermen, divers, and fishmongers. We obtained a total of 47 records, encompassing 18 species belonging to 18 different fish families. Considering existing literature, some species appear to prefer this part of the Mediterranean Sea, likely due to the Ligurian Sea’s unique hydrodynamic and oceanographic conditions that support nutrient-rich environments. Others appear to be less common in the region, probably due to the lower temperatures of the Ligurian Sea compared to other Mediterranean sectors. Our findings emphasize the dual and controversial role of the Ligurian Sea as a refuge for cold-adapted species and a hotspot for thermophilic invaders. Moreover, this research highlights the role of citizen science in complementing traditional survey approaches, providing an efficient tool to monitor many taxa across several diverse ecological niches. Full article

Glioblastoma (GBM) is an exceedingly aggressive primary brain tumor defined by rapid growth, extensive infiltration, and resistance to standard therapies. A central factor driving these malignancies is the subpopulation of glioblastoma stem cells (GSCs), which possess self-renewal capacity, multipotency, and the ability to regenerate tumor heterogeneity. GSCs contribute to key hallmarks of GBM pathobiology, including relentless progression, resistance to chemotherapy and radiotherapy, and inevitable recurrence. GSCs exhibit distinct molecular signatures, enhanced DNA repair, and metabolic adaptations that protect them against conventional treatments. Moreover, they reside within specialized niches—such as perivascular or hypoxic microenvironments—that sustain stemness, promote immunosuppression, and facilitate angiogenesis. Recent discoveries highlight signaling pathways like Notch, Wnt/β-catenin, Hedgehog, STAT3-PARN, and factors such as TFPI2 and HML-2 as critical regulators of GSC maintenance, plasticity, and immune evasion. These findings underscore the complexity of GSC biology and their pivotal role in driving GBM heterogeneity and therapeutic failure. Emerging therapeutic strategies aim to target GSCs through multiple avenues, including surface markers, immunotherapeutics (e.g., CAR T cells), metabolic vulnerabilities, and combination regimens. Advances in patient-derived organoids, single-cell omics, and 3D co-culture models enable more accurate representation of the tumor ecosystem and personalized therapeutic approaches. Ultimately, improved understanding of GSC-specific targets and the tumor microenvironment promises more effective interventions, paving the way toward better clinical outcomes for GBM patients. Full article

The human microbiome plays a vital role in maintaining human homeostasis, acting as a key regulator of host immunity and defense mechanisms. However, dysbiotic microbial communities may cause disruption of the symbiotic relationship between the host and the local microbiota, leading to the pathogenesis of various diseases, including viral infections and cancers. One of the most common infectious agents causing cancer is the human papilloma virus (HPV), which accounts for more than 90% of cervical cancers. In most cases, the host immune system is activated and clears HPV, whereas in some cases, the infection persists and can lead to precancerous lesions. Over the last two decades, the advent of next-generation sequencing (NGS) technology and bioinformatics has allowed a thorough and in-depth analysis of the microbial composition in various anatomical niches, allowing researchers to unveil the interactions and the underlying mechanisms through which the human microbiota could affect HPV infection establishment, persistence, and progression. Accordingly, the present narrative review aims to shed light on our understanding of the role of the human microbiome in the context of HPV infection and its progression, mainly to cervical cancer. Furthermore, we explore the mechanisms by which the composition and balance of microbial communities exert potential pathogenic or protective effects, leading to either HPV persistence and disease outcomes or clearance. Special interest is given to how the microbiome can modulate host immunity to HPV infection. Lastly, we summarize the latest findings on the therapeutic efficacy of probiotics and prebiotics in preventing and/or treating HPV infections and the potential of vaginal microbiota transplantation while highlighting the significance of personalized medicine approaches emerging from NGS-based microbiome profiling and artificial intelligence (AI) for the optimal management of HPV-related diseases. Full article

A significant amount of fungicides consumed in European agriculture are used in viticulture, despite vineyards only occupying a very limited percentage of the cultivated area. Cultivation of disease-resistant hybrid grape cultivars can reduce vineyard treatments by half, with a significant decrease in climate-altering emissions, farmer costs, occupational injury risks, and excessive soil compression. The objective of this study is to investigate the motives and barriers that winegrowers in South Tyrol, Italy face when considering the conversion to fungus-resistant grape varieties (PIWI) farming. We conducted a qualitative study using in-depth interviews and participant observation with winegrowers to gain insight into their perspectives on the reasons for and challenges in adopting or practicing PIWI viticulture. The paper tests two hypotheses: the environmental commitment and concerns of producers with their personal values and environmental ethics in adopting PIWI varieties and relational and social influences, leading producers towards the consideration of social sustainability. The results present a picture aligned with the existing literature, emphasizing a stronger commitment to environmental sustainability while also considering economic and legislative constraints in the cultivation of a niche wine variety, which still has a limited market presence and distribution. Full article

Pseudomonas aeruginosa is associated with both community and hospital-acquired infections. It colonizes the lungs of cystic fibrosis (CF) patients, establishing an ecological niche where it adapts and evolves from early to chronic stages, resulting in deteriorating lung function and frequent exacerbations. With antibiotics resistance on the rise, there is a pressing need for alternative personalized treatments (such as bacteriophage therapy) to combat P. aeruginosa infections. In this study, we aimed to isolate and characterize phages targeting both early and chronic P. aeruginosa isolates and evaluate their potential for phage therapy. Four highly virulent phages belonging to myoviral, podviral, and siphoviral morphotypes were isolated from sewage samples. These phages have a broad host range and effectively target 62.5% of the P. aeruginosa isolates with a positive correlation to the early isolates. All the phages have a virulence index of ≥0.90 (0.90–0.98), and one has a large burst size of 331 PFU/cell and a latency period of 30 min. All phages are stable under a wide range of temperature and pH conditions. Genomic analysis suggests the four phages are strictly lytic and devoid of identifiable temperate phage repressors and genes associated with antibiotic resistance and virulence. More significantly, two of the phages significantly delayed the onset of larval death when evaluated in a lethal Galleria mellonella infection model, suggesting their promise as phage therapy candidates for P. aeruginosa infections. Full article

Forest areas have recently become increasingly popular for physical activity in society, especially among niche sports enthusiasts. We analysed the attitude of the specific social group of cross-country skiers in Poland to pay for recreation in forest areas using their Willingness-To-Pay (WTP) and Willingness-To-Accept (WTA) declarations, which was endorsed by classification and regression tree (CART) analysis. In January–March 2023, we surveyed 50 (in a pilot study) and 255 (in the main survey) cross-country skiers, of whom 117 declared both their WTP and WTA amounts. The investigated explanatory variables included gender, age, education, residency, employment in the forestry sector, and respondents’ income or engagement in skiing. The average WTP and WTA values equalled PLN 68.6 ± 46.4 and PLN 81.3 ± 59.0/person, respectively. Despite apparent differences in the distribution of the declared WTP and WTA amounts, their medians differed only insignificantly. We found a significant correlation only between the WTP value and respondents’ income per capita, and between WTP and WTA. The CART models showed that WTP and WTA levels depended primarily on the frequency of skiing, with higher values declared by less frequent visitors. At the current respondent income level, the expenses for skiing were related the most to the respondents’ age and the frequency of skiing. In the case of increased income, they were related mostly to the respondents’ age and place of residence. The research provides practical information for forest managers in the field of recreational access to forests for people who spend their time actively in forests. Full article