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14 pages, 381 KiB  
Article
A Cross-Sectional Analysis of Oil Pulling on YouTube Shorts
by Jun Yaung, Sun Ha Park and Shahed Al Khalifah
Dent. J. 2025, 13(7), 330; https://doi.org/10.3390/dj13070330 - 21 Jul 2025
Viewed by 458
Abstract
Objective: This cross-sectional content analysis aimed to investigate how oil pulling is portrayed on YouTube Shorts, focusing on the types of speakers, claims made, and alignment with scientific evidence. The study further explored how the content may influence viewer perception, health behaviors, [...] Read more.
Objective: This cross-sectional content analysis aimed to investigate how oil pulling is portrayed on YouTube Shorts, focusing on the types of speakers, claims made, and alignment with scientific evidence. The study further explored how the content may influence viewer perception, health behaviors, and the potential spread of misinformation. Methods: On 28 January 2025, a systematic search of YouTube Shorts was performed using the term “oil pulling” in incognito mode to reduce algorithmic bias. English language videos with at least 1000 views were included through purposive sampling. A total of 47 Shorts met the inclusion criteria. Data were extracted using a structured coding framework that recorded speaker type (e.g., dentist, hygienist, influencer), engagement metrics, stated benefits, oil type and regimen, the use of disclaimers or citations, and stance toward oil pulling rated on a 5-point Likert scale. Speaker background and nationality were determined through publicly available channel descriptions or linked websites, with user identities anonymized and ethical approval deemed unnecessary due to the use of publicly available content. In total, 47 videos met the inclusion criteria. Results: Of the 47 YouTube Shorts that met the inclusion criteria, most were posted by influencers rather than dental professionals. These videos predominantly encouraged oil pulling, often recommending coconut oil for 10–15 min daily and citing benefits such as reduced halitosis and improved gum health. However, a smaller subset advanced more extreme claims, including reversing cavities and remineralizing enamel. Notably, US-licensed dentists and dental hygienists tended to discourage or express skepticism toward oil pulling, assigning lower Likert scores (1 or 2) to influencers and alternative health practitioners (often 4 or 5). Conclusions: YouTube Shorts largely promote oil pulling through anecdotal and testimonial-driven content, often diverging from evidence-based dental recommendations. The findings reveal a disconnect between professional dental guidance and popular social media narratives. While some benefits like halitosis reduction may have limited support, exaggerated or misleading claims may result in improper oral hygiene practices. Greater engagement from dental professionals and improved health communication strategies are needed to counteract misinformation and reinforce oil pulling’s role, if any, as an adjunct—not a replacement—for standard oral care. Future studies should explore viewer interpretation, behavioral influence, and cross-platform content patterns to better understand the impact of short-form health videos. Full article
(This article belongs to the Topic Preventive Dentistry and Public Health)
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15 pages, 551 KiB  
Article
Efficacy of Sesame-Based Oil Pulling in Plaque Reduction: A Randomized Controlled Trial
by Christine Zürcher, Markus Nagl, Kristian Vukoje, Ingrid Heller, Sigrun Eick and Ines Kapferer-Seebacher
Healthcare 2025, 13(14), 1634; https://doi.org/10.3390/healthcare13141634 - 8 Jul 2025
Viewed by 1019
Abstract
Objectives: To evaluate and compare the plaque-reducing efficacy of sesame-based oil pulling versus distilled water in a randomized controlled, examiner-blinded parallel-group study. Materials and Methods: Forty participants with gingivitis (community periodontal index of treatment needs grade 1 or 2) were randomly assigned to [...] Read more.
Objectives: To evaluate and compare the plaque-reducing efficacy of sesame-based oil pulling versus distilled water in a randomized controlled, examiner-blinded parallel-group study. Materials and Methods: Forty participants with gingivitis (community periodontal index of treatment needs grade 1 or 2) were randomly assigned to either the test group (sesame-based oil) or the control group (distilled water). Participants were instructed to perform oil pulling daily in the morning for 15 min over an eight-week period. The Rustogi Modified Navy Plaque Index (RMNPI) and gingival bleeding index (GBI) were evaluated at the baseline, as well as after four and eight weeks. Additionally, biofilm samples were collected for microbiological analysis. Results: The RMNPI was statistically significantly reduced after eight weeks of pulling with sesame-based oil (p < 0.001), as well as with distilled water (p < 0.001), without a significant difference between the groups. The GBI was statistically significantly reduced after eight weeks of pulling with sesame-based oil (p < 0.002), as well as with distilled water (p < 0.002), without a significant difference between the groups. No significant microbiological changes were detected in biofilm samples. Conclusions: Both plaque and gingival indices significantly decreased with oil pulling after eight weeks of intervention. Preclinical studies are necessary to clarify the mechanism of plaque reduction by oil pulling. Full article
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17 pages, 3303 KiB  
Article
Research on High-Performance Underwater-Curing Polymer Composites for Offshore Oil Riser Pipes
by Xuan Zhao, Jun Wan, Xuefeng Qv, Yajun Yu and Huiyan Zhao
Polymers 2025, 17(13), 1827; https://doi.org/10.3390/polym17131827 - 30 Jun 2025
Viewed by 422
Abstract
In offshore oil and gas extraction, riser pipes serve as the first isolation barrier for wellbore integrity, playing a crucial role in ensuring operational safety. Protective coatings represent an effective measure for corrosion prevention in riser pipes. To address issues such as electrochemical [...] Read more.
In offshore oil and gas extraction, riser pipes serve as the first isolation barrier for wellbore integrity, playing a crucial role in ensuring operational safety. Protective coatings represent an effective measure for corrosion prevention in riser pipes. To address issues such as electrochemical corrosion and poor adhesion of existing coatings, this study developed an underwater-curing composite material based on a polyisobutylene (PIB) and butyl rubber (IIR) blend system. The material simultaneously exhibits high peel strength, low water absorption, and stability across a wide temperature range. First, the contradiction between material elasticity and strength was overcome through the synergistic effect of medium molecular weight PIB internal plasticization and IIR crosslinking networks. Second, stable peel strength across a wide temperature range (−45 °C to 80 °C) was achieved by utilizing the interfacial effects of nano-fillers. Subsequently, an innovative solvent-free two-component epoxy system was developed, combining medium molecular weight PIB internal plasticization, nano-silica hydrogen bond reinforcement, and latent curing agent regulation. This system achieves rapid surface drying within 30 min underwater and pull-off strength exceeding 3.5 MPa. Through systematic laboratory testing and field application experiments on offshore oil and gas well risers, the material’s fundamental properties and operational performance were determined. Results indicate that the material exhibits a peel strength of 5 N/cm on offshore oil risers, significantly extending the service life of the riser pipes. This research provides theoretical foundation and technical support for improving the efficiency and reliability of repair processes for offshore oil riser pipes. Full article
(This article belongs to the Special Issue Advances in Functional Polymers and Composites: 2nd Edition)
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19 pages, 2253 KiB  
Article
A Push–Pull Strategy to Enhance Biomass and Lipid Production in Nannochloropsis oculata
by Roxana Guadalupe Tamayo-Castañeda, Gloria Viviana Cerrillo-Rojas, Teodoro Ibarra-Pérez, Christophe Ndjatchi and Hans Christian Correa-Aguado
Microorganisms 2025, 13(5), 1131; https://doi.org/10.3390/microorganisms13051131 - 15 May 2025
Viewed by 656
Abstract
The high demand for sustainable biodiesel feedstocks has led to the exploration of innovative strategies to enhance lipid productivity in microalgae. This study introduces a push–pull strategy to optimize lipid accumulation in Nannochloropsis oculata. The benzyl amino purine (BAP) and naphthalene acetic [...] Read more.
The high demand for sustainable biodiesel feedstocks has led to the exploration of innovative strategies to enhance lipid productivity in microalgae. This study introduces a push–pull strategy to optimize lipid accumulation in Nannochloropsis oculata. The benzyl amino purine (BAP) and naphthalene acetic acid (NAA) stimulation, acting as the ‘push’ component, significantly boost growth and nutrient stress tolerance. Meanwhile, the ‘pull’ component, nitrogen (N) deficiency, triggers lipid biosynthesis. A Box–Behnken design was employed to optimize the factors named BAP fraction (0–1), total phytohormone (PH) BAP/NAA mix dose (0–20 ppm), and N-concentration (0–50%). The combined BAP/NAA treatment significantly increased biomass (15% higher than the control) and mitigated N-stress with higher doses (20 ppm). Lipid yield surged from 12.4% to 38.87% under optimized conditions (23.25% N, 39.5 ppm NAA, and BAP fraction 0). The push–pull strategy contributed to boosting lipid synthesis and balancing biomass production. N-limitation and total PH dosage were the determining factors in this strategy. This work demonstrates the potential of the push–pull strategy in increasing lipid accumulation, offering a promising and optimistic solution for biodiesel production at scale from microalgae. By reducing dependence on fossil fuels, N. oculata emerges as a reliable feedstock for oil extraction and biodiesel. Full article
(This article belongs to the Special Issue The Application Potential of Microalgae in Green Biotechnology)
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24 pages, 26458 KiB  
Article
An Innovative Tube Hydro-Joining Process Combining Piercing, Hole Flanging and Nut Inlaying
by Yeong-Maw Hwang, Hong-Nhan Pham, Ze-Wei Ho and Yu-Jen Wang
Materials 2025, 18(9), 1990; https://doi.org/10.3390/ma18091990 - 28 Apr 2025
Viewed by 473
Abstract
This paper proposes a novel tube hydro-joining process, which combines piercing, hole flanging, and nut inlaying. The nut punch shape design proposed by this paper can deliver three advantages of no scrap, no oil leakage, and longer flange length, which can achieve stronger [...] Read more.
This paper proposes a novel tube hydro-joining process, which combines piercing, hole flanging, and nut inlaying. The nut punch shape design proposed by this paper can deliver three advantages of no scrap, no oil leakage, and longer flange length, which can achieve stronger clamping force and accordingly increase the pull out load. First, we use the finite element analysis to investigate the elasto-plastic deformation of the aluminum alloy A6063 tube during the hydro-joining process. A punch-shaped nut with a tapered locking part is designed to increase the elasto-binding strength of the pierced tube and the pull out load of the inlayed nut. The effects of hydro-joining loading paths on the formability of the A6063 tubes and punch-shaped nuts are examined. Additionally, the effects of fit zone size, nut punch stroke length, internal pressure, nut diameter, and the die hole diameter on the pull out load and twisting torque are explored. Finally, experiments on hydro-joining of A6063 tubes are conducted to validate the finite element modeling and the simulation results. Full article
(This article belongs to the Collection Welding and Joining Processes of Materials)
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13 pages, 285 KiB  
Review
Hematophagous Tick Control in the South African Cattle Production System by Using Fossil Shell Flour as a Sustainable Solution: A Systematic Review
by Zimkhitha Soji-Mbongo, Olusegun O. Ikusika and Thando C. Mpendulo
Sustainability 2025, 17(7), 2826; https://doi.org/10.3390/su17072826 - 22 Mar 2025
Viewed by 389
Abstract
Ticks pose one of the key economic risks to the cattle industry globally, affecting productivity, health, and welfare. Over 80% of the cattle population around the globe is affected by tick infestation. Several tick control methods, including the use of chemical acaricides, herbal [...] Read more.
Ticks pose one of the key economic risks to the cattle industry globally, affecting productivity, health, and welfare. Over 80% of the cattle population around the globe is affected by tick infestation. Several tick control methods, including the use of chemical acaricides, herbal agents, and some complementary measures, like the use of old motor oil, disinfectant, pour-on, tick grease, pulling off, cutting, paraffin, and Jeyes fluid, have been used by farmers to alleviate the effects of hematophagous ticks. However, these strategies are often mistakenly employed and can harm animals. Likewise, these methods cannot be sustained because of their cost, environmental impact, flaws, and resistance. An abundantly available, cost-effective, environmentally friendly, and naturally occurring substance like fossil shell flour with no known side effects could present a sustainable solution. This review abridged the research and information on hematophagous tick control in South African cattle production using fossil shell flour. This is a systematic review of the published literature and catalogues. All available documented evidence on this topic was collated and synthesized through standardized methods of systematic review protocol. Different scientific studies and a few references from farmers’ magazines published from 1941 to 2022 were reviewed. Out of 120 research papers downloaded, 98 were included and analyzed directly or indirectly regarding hematophagous tick control in cattle production and the use of fossil shell flour among livestock farmers. The advancement in ectoparasite control through fossil shell flour is a novel concept that needs to be explored for the benefit of all livestock farmers, hence this review. Fossil shell flour has been shown to have good insecticidal effectiveness against insects of animal and agricultural importance. We hereby recommend the exploration of FSF as an alternative tick control measure to the currently used acaricides to which ticks have developed resistance. Full article
20 pages, 6955 KiB  
Article
Numerical Simulation and Experimental Testing of the Strength of the Piston-Returning Spherical Joint Pair in a High-Speed Axial Piston Pump
by Shaonian Li, Fuhao Li, Longtao Yang, Kaifeng Wang and Liting Fu
Processes 2025, 13(2), 417; https://doi.org/10.3390/pr13020417 - 5 Feb 2025
Viewed by 633
Abstract
The piston-returning spherical joint pair in an axial piston pump continuously bears alternating loads generated by conversions between high and low pressure. If its strength fails, then the axial piston pump cannot function normally. Therefore, we performed numerical simulations and laboratory experiments to [...] Read more.
The piston-returning spherical joint pair in an axial piston pump continuously bears alternating loads generated by conversions between high and low pressure. If its strength fails, then the axial piston pump cannot function normally. Therefore, we performed numerical simulations and laboratory experiments to investigate the strength properties of the piston-returning spherical joint pair components of an axial piston pump. The results show that when the piston is in the transition area from oil suction to oil discharge, the maximum deformation and stress of the slipper are located on the inner surface of the slipper spherical socket, and the maximum deformation value is 2.523 μm. When the piston is in the transition area from oil discharge to oil suction, the maximum deformation and stress of the slipper are located at the closing part of the slipper, and the maximum deformation value is 1.959 μm. The maximum deformation of the piston at both positions is located at the bottom of the piston, with values of 11.622 μm and 3.8512 μm, respectively. The maximum stress of the piston is located in the neck of the piston. The deformation at the spherical socket closure of the slipper increases with the increase in the pushing–pulling force, and this relationship is nonlinear. The maximum deformation at the spherical socket closure is smallest for the manganese brass slipper, is larger for the tin bronze slipper, and is largest for the ordinary brass slipper. The maximum deformation at the spherical socket closure of the slipper obtained by the strength test is greater than the simulation result. These research conclusions can serve as a reference for the design of piston-returning spherical joint pairs in axial piston pumps. Full article
(This article belongs to the Section Energy Systems)
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21 pages, 24481 KiB  
Article
Cold-Drawn Wood-Filled Polybutylene Succinate Macro-Fibers as a Reinforcing Material for Concrete
by Jonas Herz, Verena Schusser, Dirk Muscat and Nicole Strübbe
Polymers 2025, 17(3), 403; https://doi.org/10.3390/polym17030403 - 3 Feb 2025
Cited by 1 | Viewed by 902
Abstract
The corrosive behavior of steel reinforcements causes issues in the concrete industry. To overcome this issue, alternative noncorrosive reinforcements such as polymer fibers could be used. However, as environmental protection becomes more important, sustainability must also be considered in the solution. An alternative [...] Read more.
The corrosive behavior of steel reinforcements causes issues in the concrete industry. To overcome this issue, alternative noncorrosive reinforcements such as polymer fibers could be used. However, as environmental protection becomes more important, sustainability must also be considered in the solution. An alternative to polymers based on raw oil is bio-based polymers. This study investigates the suitability of polymer fibers produced from polybutylene succinate together with cellulose and wood fillers as concrete reinforcements. Different mixtures of polybutylene succinate, cellulose, and wood fillers were created, and fibers were produced using a multiple drawing process. The fibers were tested using tensile tests, a single-fiber pull-out test, contact angle measurements, reflected light microscopy, density measurements, and thermogravimetric analysis. The fillers were shown to decrease the mechanical properties as the particle size and filler amount increased, resulting in a reduction in Young’s modulus and tensile strength of 55% and 70%, respectively, while adhesion to concrete increased with particle size from 0.31 ± 0.02 N/mm2 without filler to 0.90 ± 0.10 N/mm2 for the best-performing material combination. Reflected light microscopy images show changes in the fiber surface before and after pull-out. The fiber density decreased from 1.26 ± 0.05 g/cm3 to 0.91 ± 0.04 g/cm3 with an increasing filler amount and particle size for a compound with 10 weight percent of wood filler 1. The fiber thermal stability decreased slightly with the addition of filler. The greatest effect was a reduction in the temperature to ≈58 °C at 1% weight loss when 10 weight percent of wood was added. This study proves the possibility of using bio-based materials as concrete reinforcements. Full article
(This article belongs to the Section Polymer Fibers)
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15 pages, 1551 KiB  
Article
Sex Differences in the Anxiolytic Properties of Common Cannabis Terpenes, Linalool and β-Myrcene, in Mice
by Jasmin K. Wagner, Ella Gambell, Tucker Gibbons, Thomas J. Martin and Joshua S. Kaplan
NeuroSci 2024, 5(4), 635-649; https://doi.org/10.3390/neurosci5040045 - 3 Dec 2024
Cited by 2 | Viewed by 1949
Abstract
Volatile organic compounds, colloquially referred to as “terpenes”, have been proposed to impact the therapeutic qualities that are traditionally ascribed to cannabis. However, the contribution of these terpenes in anxiety, at relevant levels and exposure methods common with cannabis use, is lacking empirical [...] Read more.
Volatile organic compounds, colloquially referred to as “terpenes”, have been proposed to impact the therapeutic qualities that are traditionally ascribed to cannabis. However, the contribution of these terpenes in anxiety, at relevant levels and exposure methods common with cannabis use, is lacking empirical assessment. We tested the anxiolytic properties of two prominent cannabis terpenes, linalool and β-myrcene, in male and female mice using short duration vapor pulls to model human inhalation when combusting flower or vaping cannabis oil. We observed sex differences in the locomotor effects in the open field and anxiolytic properties in the elevated plus maze of these terpenes that depended on their exposure characteristics. Both linalool and β-myrcene had anxiolytic effects in female mice when delivered in discrete vapor pulls over the course of 30 min. In male mice, only a single vapor hit containing linalool or β-myrcene had anxiolytic effects. The combination of sub-effective levels of linalool and the phytocannabinoid, cannabidiol (CBD), had synergistic anxiolytic effects in females, but these entourage effects between CBD and terpenes were absent with β-myrcene for females and for either terpene in males. Together, our findings reveal sex differences in the anxiolytic properties of common cannabis terpenes and highlight the potential benefits of unique combinations of CBD and terpenes in expanding the therapeutic dose window. Full article
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15 pages, 4206 KiB  
Article
Reinforcement of Epoxidized Natural Rubber with High Antimicrobial Resistance Using Water Hyacinth Fibers and Chlorhexidine Gluconate
by Thidarat Kanthiya, Pornchai Rachtanapun, Siwarote Boonrasri, Thorsak Kittikorn, Thanongsak Chaiyaso, Patnarin Worajittiphon, Nuttapol Tanadchangsaeng, Sarinthip Thanakkasaranee, Noppol Leksawasdi, Yuthana Phimolsiripol, Warintorn Ruksiriwanich and Kittisak Jantanasakulwong
Polymers 2024, 16(21), 3089; https://doi.org/10.3390/polym16213089 - 31 Oct 2024
Cited by 2 | Viewed by 1572
Abstract
In this study, epoxidized natural rubber (ENR) was mixed using a two-roller mixer. Water hyacinth fiber (WHF) acted as a reinforcing agent in the preparation of the rubber composite at 10 phr (ENRC/WHF). Chlorhexidine gluconate (CHG) was added at different concentrations (1, 5, [...] Read more.
In this study, epoxidized natural rubber (ENR) was mixed using a two-roller mixer. Water hyacinth fiber (WHF) acted as a reinforcing agent in the preparation of the rubber composite at 10 phr (ENRC/WHF). Chlorhexidine gluconate (CHG) was added at different concentrations (1, 5, 10, and 20 phr) as an antimicrobial and coupling agent. The tensile strength increased with a CHG content of 1 phr (4.59 MPa). The ENRC/WHF/CHG20 blend offered high hardness (38) and good morphology owing to the reduction in cavities and fiber pull-out from the rubber matrix. The swelling of the sample blends in oil and toluene decreased as the CHG content increased. Reactions of –NH2/epoxy groups and –NH2/–OH groups occurred during the preparation of the ENRC/WHF/CHG blend. The FTIR spectroscopy peak at 1730 cm−1 confirmed the reaction between the −NH2 groups of CHG and epoxy groups of ENR. The ENRC/WHF/CHG blend at 10 phr and 20 phr exhibited zones of inhibition against three bacterial species (Staphylococcus aureus, Escherichia coli, and Bacillus cereus). CHG simultaneously acted as a crosslinking agent between ENR and WHF and as an antimicrobial additive for the blends. CHG also improved the tensile strength, hardness, swelling, and antimicrobial properties of ENR composites. Full article
(This article belongs to the Special Issue Mechanical and Structural Behavior for Polymer Composites)
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37 pages, 1678 KiB  
Review
Oral Microbiome: A Review of Its Impact on Oral and Systemic Health
by John J. Rajasekaran, Hari Krishnan Krishnamurthy, Jophi Bosco, Vasanth Jayaraman, Karthik Krishna, Tianhao Wang and Kang Bei
Microorganisms 2024, 12(9), 1797; https://doi.org/10.3390/microorganisms12091797 - 29 Aug 2024
Cited by 36 | Viewed by 28736
Abstract
Purpose of review: This review investigates the oral microbiome’s composition, functions, influencing factors, connections to oral and systemic diseases, and personalized oral care strategies. Recent findings: The oral microbiome is a complex ecosystem consisting of bacteria, fungi, archaea, and viruses that contribute to [...] Read more.
Purpose of review: This review investigates the oral microbiome’s composition, functions, influencing factors, connections to oral and systemic diseases, and personalized oral care strategies. Recent findings: The oral microbiome is a complex ecosystem consisting of bacteria, fungi, archaea, and viruses that contribute to oral health. Various factors, such as diet, smoking, alcohol consumption, lifestyle choices, and medical conditions, can affect the balance of the oral microbiome and lead to dysbiosis, which can result in oral health issues like dental caries, gingivitis, periodontitis, oral candidiasis, and halitosis. Importantly, our review explores novel associations between the oral microbiome and systemic diseases including gastrointestinal, cardiovascular, endocrinal, and neurological conditions, autoimmune diseases, and cancer. We comprehensively review the efficacy of interventions like dental probiotics, xylitol, oral rinses, fluoride, essential oils, oil pulling, and peptides in promoting oral health by modulating the oral microbiome. Summary: This review emphasizes the critical functions of the oral microbiota in dental and overall health, providing insights into the effects of microbial imbalances on various diseases. It underlines the significant connection between the oral microbiota and general health. Furthermore, it explores the advantages of probiotics and other dental care ingredients in promoting oral health and addressing common oral issues, offering a comprehensive strategy for personalized oral care. Full article
(This article belongs to the Section Microbiomes)
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21 pages, 19755 KiB  
Article
Pin1 Exacerbates Non-Alcoholic Fatty Liver Disease by Enhancing Its Activity through Binding to ACC1
by Yiyi Jin, Zhaoshui Shangguan, Jiao Pang, Yuwen Chen, Suijin Lin and Hekun Liu
Int. J. Mol. Sci. 2024, 25(11), 5822; https://doi.org/10.3390/ijms25115822 - 27 May 2024
Cited by 3 | Viewed by 2329
Abstract
Non-alcoholic fatty liver disease (NAFLD) is a clinicopathological syndrome characterized by diffuse hepatocellular steatosis due to fatty deposits in hepatocytes, excluding alcohol and other known liver injury factors. However, there are no specific drugs for the clinical treatment of NAFLD. Therefore, research on [...] Read more.
Non-alcoholic fatty liver disease (NAFLD) is a clinicopathological syndrome characterized by diffuse hepatocellular steatosis due to fatty deposits in hepatocytes, excluding alcohol and other known liver injury factors. However, there are no specific drugs for the clinical treatment of NAFLD. Therefore, research on the pathogenesis of NAFLD at the cellular and molecular levels is a promising approach to finding therapeutic targets and developing targeted drugs for NAFLD. Pin1 is highly expressed during adipogenesis and contributes to adipose differentiation, but its specific mechanism of action in NAFLD is unclear. In this study, we investigated the role of Pin1 in promoting the development of NAFLD and its potential mechanisms in vitro and in vivo. First, Pin1 was verified in the NAFLD model in vitro using MCD diet-fed mice by Western Blot, RT-qPCR and immunohistochemistry (IHC) assays. In the in vitro study, we used the oleic acid (OA) stimulation-induced lipid accumulation model and examined the lipid accumulation in each group of cells by oil red O staining as well as BODIPY staining. The results showed that knockdown of Pin1 inhibited lipid accumulation in hepatocytes in an in vitro lipid accumulation model and improved lipid indices and liver injury levels. Moreover, in vivo, WT and Pin1-KO mice were fed a methionine-choline deficient (MCD) diet for 4 weeks to induce the NAFLD model. The effects of Pin1 on lipid accumulation, hepatic fibrosis, and oxidative stress were evaluated by biochemical analysis, glucose and insulin tolerance tests, histological analysis, IHC, RT-qPCR and Western blot assays. The results indicate that Pin1 knockdown significantly alleviated hepatic steatosis, fibrosis and inflammation in MCD-induced NAFLD mice, improved glucose tolerance and alleviated insulin resistance in mice. Further studies showed that the AMPK/ACC1 signalling pathway might take part in the process by which Pin1 regulates NAFLD, as evidenced by the inhibition of the AMPK/ACC1 pathway. In addition, immunofluorescence (IF), coimmunoprecipitation (Co-IP) and GST pull-down experiments also showed that Pin1 interacts directly with ACC1 and inhibits ACC1 phosphorylation levels. Our study suggests that Pin1 promotes NAFLD progression by inhibiting the activation of the AMPK/ACC1 signalling pathway, and it is possible that this effect is achieved by Pin1 interacting with ACC1 and inhibiting the phosphorylation of ACC1. Full article
(This article belongs to the Section Molecular Endocrinology and Metabolism)
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21 pages, 4750 KiB  
Article
Formation Mechanism of NW-Trending Faults and Their Significance on Basin Evolution in Zhu III Depression of the Pearl River Mouth Basin, SE China
by Pengfei Zhu, Lintao Zhao, Jiantai Zhang, Dunling Mu, Yichun Chen and Pengfei Rong
J. Mar. Sci. Eng. 2024, 12(6), 858; https://doi.org/10.3390/jmse12060858 - 22 May 2024
Cited by 1 | Viewed by 1674
Abstract
The Zhu III Depression, situated in the northern Pearl River Mouth Basin, features a complex fault system composed of NE–SW-, nearly E–W-, and NW–SE-oriented faults. However, there is limited research on NW-trending faults, especially regarding their formation mechanisms. Through structural analysis of 3D [...] Read more.
The Zhu III Depression, situated in the northern Pearl River Mouth Basin, features a complex fault system composed of NE–SW-, nearly E–W-, and NW–SE-oriented faults. However, there is limited research on NW-trending faults, especially regarding their formation mechanisms. Through structural analysis of 3D seismic profiles, we have revealed the geometric and kinematic characteristics of NW-trending faults and categorized them into three types based on their formation mechanisms: extensional fault, dextral transtensional fault, and sinistral strike–slip fault. The extensional faults predominantly developed as boundary faults during the rifting I period, caused by tectonic inversion of the NW–NWW-trending basement faults since early Eocene. The transtensional fault resulted from the dextral strike–slip motion of the NE-trending basin-controlling faults since late Eocene, under the regional dextral extension stress setting. The sinistral strike–slip faults have been dominant during the post-rifting period since early Oligocene. This is due to the sinistral shearing action related to the southeastward lateral extrusion of the Indochina Block and slab pull southward by subduction of the proto-SCS. The NW-trending faults controlled the development of local tectonics and structures, the depocenter migration during the rifting period, and the trapping, migration, and preservation of oil and gas. Full article
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23 pages, 12234 KiB  
Article
A Laboratory Machine Verifying the Operation of a Hydraulic Rope Equalizer with Tensometric Sensors
by Leopold Hrabovský, Jiří Fries and Štěpán Pravda
Sensors 2024, 24(8), 2588; https://doi.org/10.3390/s24082588 - 18 Apr 2024
Viewed by 1585
Abstract
In mining machines with friction discs, but also in multi-rope traction elevators, it is necessary to distribute the applied tensile load, generated by the weight of the cage and counterweight, evenly in all cross-sections of the load-bearing ropes. Hydraulic devices used for this [...] Read more.
In mining machines with friction discs, but also in multi-rope traction elevators, it is necessary to distribute the applied tensile load, generated by the weight of the cage and counterweight, evenly in all cross-sections of the load-bearing ropes. Hydraulic devices used for this purpose can operate on the principle of Pascal’s law. This article presents a structural design, a 3D model and an implemented solution of a laboratory device capable of simulating a practical method of evenly distributing the total weight of the load into partial tensile forces of the same size acting on a selected number of load-bearing ropes. The laboratory equipment uses two pairs of three steel cables of finite length for the simulations. During the experimental measurements, tensile forces derived from the tractive force of the piston rods, pushed into the bodies of the hydraulic cylinders by the pressure of the hydraulic oil supplied through the pipeline under the pistons of the hydraulic cylinders, were detected. The resulting amount of hydraulic oil pressure in the hydraulic circuit influenced by different values of the hydraulic oil pressures in the hydraulic cylinders and by the pressure in the supply pipe was experimentally studied on the laboratory equipment. Simulations were also carried out in order to detect the hydraulic oil pressure in the hydraulic circuit caused by the change in the different magnitudes of the tensile forces in the ropes. From the experiments carried out, it follows that with the appropriate choice of hydraulic elements and the design of the hydraulic circuit, the weight of the load, acting as the total pulling force in the ropes, can be evenly distributed (with a deviation of up to 5%) to all cross-sections of the load-bearing ropes. If the exact values of the hydraulic oil volumes under the pistons of all hydraulic cylinders are not known, it is not possible to calculate the pressure values in the hydraulic circuit when the valves of the hydraulic pipes are gradually opened. Full article
(This article belongs to the Section Physical Sensors)
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17 pages, 5420 KiB  
Article
Coatings Adhesion on Chemically Modified Scots Pine (Pinus sylvestris L.) Woods
by Adefemi Adebisi Alade, Christoph Hoette and Holger Militz
Forests 2024, 15(3), 526; https://doi.org/10.3390/f15030526 - 13 Mar 2024
Cited by 3 | Viewed by 1436
Abstract
Synergizing coating and wood modification is a promising concept to develop wood products that have multi-qualities that include excellent dimensional stability, durability, and weathering resistance. However, the nature of the modified substrate is a critical parameter for coating adhesion. Chemical modification of wood [...] Read more.
Synergizing coating and wood modification is a promising concept to develop wood products that have multi-qualities that include excellent dimensional stability, durability, and weathering resistance. However, the nature of the modified substrate is a critical parameter for coating adhesion. Chemical modification of wood impacts the physicochemical properties of the wood, which could in turn impact the adhesion of coatings. Therefore, this study investigated the adhesion of seven different coatings to Pinus sylvestris L. woods chemically modified through esterification with acetic anhydride (acetylated), etherification with 1.3-dimethylol-4.5-dihydroxyethyleneurea (DMDHEU), and esterification with sorbitol/citric acid formulation (SorCA). The selected coatings include water-based and solvent-based examples with different binder constituents that include acrylate, alkyd, natural oil, and hybrids. Coating adhesion to the modified wood was evaluated in terms of crosscut resistance to detachment, wear-resistant hardness, and pull-off strength. Chemical modifications yielded positive impacts on coating adhesion compared to unmodified wood. Coatings adhered better to acetylated and DMDHEU-modified P. sylvestris wood than on SorCA-modified wood. Solvent-based coatings had higher adhesion strength on the acetylated, DMDHEU-modified, and unmodified woods than water-based coatings. On the other hand, water-based coatings mostly adhered better to SorCA-modified wood compared to solvent-based coating. Overall, the coating of chemically modified P. sylvestris wood is promising for the development of an enhanced wood protection system. Full article
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