Search Results (24,507)

Bee pollen is a nutrient-dense food; however, its dense cell wall limits the bioavailability and digestive absorption of nutrients. This study established a co-fermentation process that combines Lactobacillus strains isolated from bee bread with commercial probiotics to improve the nutritional profile and functional properties of bee pollen. L. acidophilus (LBA1) and L. plantarum (LBP3) were isolated from bee bread and used for single-strain fermentation of bee pollen and its co-fermentation with commercial probiotics. The results indicated that fermentation increased the protein, free amino acid, vitamin C, and flavonoid contents. The co-fermentation product (FHL-99) of LBP3 and the commercial inoculant (99 strains) exhibited the highest cell wall disruption rate (67.57%) in artificial intestinal juice. Ex vivo activity analysis revealed enhanced DPPH, hydroxyl, and ABTS⁺ radical scavenging capacities of fermented bee pollen. Its inhibitory effects on hyaluronidase activity and protein thermal denaturation were also enhanced. FHL-99 demonstrated optimal performance across multiple indices, achieving a DPPH radical scavenging rate of 77.46% and hyaluronidase inhibition rate of 37.38%. In conclusion, synergistic co-fermentation can disrupt pollen cell walls and enrich bioactive constituents, providing an efficient biotechnological approach for the development of high-quality fermented bee pollen products. Full article

Strip intercropping of maize and soybean is a key practice for improving land productivity and ensuring food and oil security in the hilly regions of the Loess Plateau. However, complex interspecific interactions generate highly heterogeneous canopy structures, making it difficult for traditional linear models to capture yield variability within mixed pixels. Based on a single-season (2025) field experiment, this study developed a UAV multispectral imagery-based yield estimation framework integrating multiple machine-learning algorithms. Shapley additive explanations (SHAP) and partial dependence plots (PDP) were used to interpret the spectral–yield relationships under different spatial configurations. The predictive performance of linear regression and eight nonlinear algorithms was compared using 20 spectral features. Ensemble learning outperformed linear approaches in all intercropping scenarios. In the maize–soybean 3:2 pattern, the GBDT model delivered the highest accuracy (R² = 0.849; NRMSE = 9.28%), whereas in the 4:2 pattern with stronger shading stress on soybean, the random forest model showed the greatest robustness (R² = 0.724). Interpretation results indicated that yield in monoculture systems was mainly driven by physiological traits characterized by visible-band indices, while yield in intercropping systems was dominated by structural and stress-response traits represented by near-infrared and soil-adjusted vegetation indices. The generated centimeter-scale yield maps revealed clear strip-like spatial variability driven by interspecific competition. Overall, explainable machine learning combined with UAV multispectral data shows promise for within-season yield estimation in intercropping systems and can support spatially differentiated precision management under the sampled conditions. Full article

This study applies a multiple threshold nonlinear autoregressive distributed lag (MTNARDL) model to examine the asymmetric impact of real exchange rate volatility on Vietnam’s exports and imports with its three leading trading partners: China, the United States, and South Korea. By allowing trade responses to vary across different volatility regimes, the MTNARDL framework provides a flexible approach to capturing potential nonlinear adjustment dynamics that cannot be addressed by single-threshold models. Moreover, using bilateral import and export data helps reduce aggregation bias. The results indicate the presence of asymmetric long-run adjustment dynamics in the relationship between real exchange rate volatility and bilateral trade flows, while short-run effects are generally weak and less consistent across trading partners. These findings provide valuable insights into the complex effects of exchange rate volatility, enabling policymakers to more effectively design and manage policies to mitigate its impact. Full article

Multicollinearity and outliers are common challenges in multiple linear regression, often adversely affecting the properties of least squares estimators. To address these issues, several robust estimators have been developed to handle multicollinearity and outliers individually or simultaneously. More recently, the robust Stein estimator (RSE) was introduced, which integrates shrinkage and robustness to effectively mitigate the impact of both multicollinearity and outliers. Despite its theoretical advantages, the finite-sample performance of this approach under multicollinearity and outliers remains underexplored. First, outliers in the y direction have been the main focus of earlier research on the RSE, not considering that leverage points could substantially impact regression results. Second, this study addresses the gap by considering outliers in the y direction and leverage points, providing a more thorough assessment of the RSE robustness. Finally, to extend the limited existing benchmark, we compare and evaluate the RSE performance with a wide range of robust and classical estimators. This extends existing benchmarking, which is limited in the current literature. Several Monte Carlo (MC) simulations were conducted, considering both normal and heavy-tailed error distributions, with sample sizes, multicollinearity levels, and outlier proportions varied. Performance was evaluated using bootstrap estimates of root mean squared error (RMSE) and bias. The MC simulation results indicated that the RSE outperformed other estimators under several scenarios where both multicollinearity and outliers are present. Finally, real data studies confirm the MC simulation results. Full article

The chlorophyll content of plant leaves measured by the soil plant analysis development (SPAD) is an important indicator for measuring crop growth status and irrigation effect. The rapid, non-destructive and efficient estimation of crop SPAD values is of great significance to the field management of crops. In this study, the canopy hyperspectral reflectance and SPAD values of winter wheat were obtained, and the spectral curve was changed through four spectral processing methods, including first-order differential (FD), second-order differential (SD), multivariate scattering correction (MSC), and Savitzky–Golay smoothing (SG) to improve the correlation between canopy spectral reflectance and SPAD. Furthermore, to investigate and evaluate the performance of various vegetation indices (VIs) in estimating SPAD values for winter wheat, existing published indices were optimized using random band combinations derived from multiple canopy spectral transformations. The optimized vegetation index was used as the input variable of the model, and six machine learning algorithms, including random forest (RF), long short-term memory network (LSTM), multilayer perceptron (MLP), deep recurrent neural network (Deep-RNN), gated recurrent unit (GRU), and convolutional neural network (CNN), were used to construct the winter wheat SPAD values estimation model, and the model was verified. The experimental results demonstrate that, when utilizing an equivalent number of optimized vegetation indices as input, the GRU-based model achieves higher estimation accuracy compared to other models. Specifically, the coefficient of determination (R²) is improved by 0.12 compared to the RF model, by 0.03 compared to the LSTM model, by 0.12 compared to the MLP model, by 0.02 compared to the Deep-RNN model, and by 0.02 compared to the CNN model. At the same time, the GRU model also has a lower root mean square error (RMSE) and relative error (RE) of 7.37 and 24.90%, respectively. This study provides valuable hyperspectral remote sensing technology support for the implementation of winter wheat SPAD values estimation in the field. Full article

Droughts are one of the most significant hazards that affect human life due to the imbalanced distribution of water across the world. Some parts of the world are usually dry, and meteorological conditions affect these regions rapidly. In water-scarce regions, droughts significantly put at risk socio-economic stability and food security, which may cause a major challenge to sustainable development. Therefore, a precise definition of drought and the identification of early warning signals can help to minimize the negative effects of droughts, especially in terms of agriculture. In this study, drought signals of three major agricultural provinces of Turkey, namely Antalya, Şanlıurfa, and Konya, were investigated. For this purpose, the Standard Precipitation Index (SPI), Standardized Precipitation Evapotranspiration Index (SPEI), Evaporative Demand Drought Index (EDDI), and Vegetation Condition Index (VCI) were computed for each province. A composite score index was proposed for the evaluation of multiple indices together. All datasets were obtained from remote-sensing products to ensure reproducibility. A dataset for the 2003–2023 period was used. The monthly precipitation derived from CHIRPS data and potential evaporation (PEV) data were obtained from the ERA5-Land. Therefore, the SPEI and EDDI values were calculated by using ERA5-Land PEV values but not the evapotranspiration. The Normalized Difference Vegetation Index (NDVI) values for each province were obtained from the MODIS/Terra MOD13A3 v061. The Mann–Kendall test and Sen’s slope were applied to the computed time series to detect the trends. As a result, the dry and wet periods were identified for each province individually. The VCI was found to have an increasing trend for all tested provinces. Overall, from a future perspective, the most vulnerable province in terms of meteorological drought was indicated to be Antalya. Full article

As network environments become increasingly interconnected, ensuring robust cyber-security has become critical, particularly with the growing sophistication of modern cyber threats. Intrusion detection systems (IDSs) play a vital role in identifying and mitigating unauthorized or malicious activities; however, conventional machine learning-based IDSs often rely on handcrafted features and are limited in their ability to detect diverse attack types across disparate network domains. To address these limitations, this paper introduces a novel unified intrusion detection framework that implements “Structural Dualism” to integrate three heterogeneous benchmark datasets (CSE-CIC-IDS2018, NF-BoT-IoT-v2, and IoT-23) into a harmonized, protocol-agnostic representation. The framework employs a shared autoencoder architecture with dataset-specific projection layers to learn a unified latent manifold. This 15-dimensional space captures the underlying semantics of attack patterns (e.g., volumetric vs. signaling) across multiple domains, while dataset-specific decoders preserve reconstruction fidelity through alternating multi-domain training. To identify complex micro-signatures within this manifold, the framework utilizes a synergistic hybrid convolutional neural network–deep neural network (CNN–DNN) classifier, where the CNN extracts spatial latent patterns and the DNN performs global classification across twenty-five distinct classes. Class imbalance is addressed through resampling strategies such as adaptive synthetic sampling (ADASYN) and edited nearest neighbors (ENN). Experimental results demonstrate remarkable performance, achieving 99.76% accuracy for binary classification and 99.54% accuracy for multi-class classification on the merged dataset, with strong generalization confirmed on individual datasets. These findings indicate that the shared autoencoder-based CNN–DNN framework, through its unique feature alignment and spatial extraction capabilities, significantly strengthens intrusion detection across diverse and heterogeneous environments. Full article

Coverage expansions and affordability reforms often presume that improved access to care yields better population health. We examine this premise in a capacity-constrained healthcare system, where congestion and throughput determine whether potential access translates into realized care. Using U.S. state-year panel data from 2006 to 2023, we study (i) how healthcare workforce density relates to multiple access margins and (ii) whether the mortality effects of access improvements depend on local delivery capacity. Reduced-form estimates show that higher workforce density is associated with higher insurance coverage and fewer cost-related barriers to care, while associations with having a usual source of care are weaker. With full controls these relationships attenuate, and Medicaid expansion and poverty explain much of the remaining variation. Instrumental variable models suggest that policy-driven improvements in effective access are associated with lower mortality, although the first-stage strength varies across specifications. Interaction-IV estimates indicate capacity dependence: for all-cause and external-cause mortality, implied benefits are larger in lower-capacity settings and diminish as workforce density increases; for endocrine mortality, benefits are concentrated in higher-capacity settings, while respiratory effects are not detectable. Overall, the results support a systems perspective in which the health returns to access expansions depend on local delivery capacity, underscoring the importance of aligning access reforms with constraints in healthcare production and flow. Full article

Underground tunnel construction projects using tunnel boring machines (TBMs) require a holistic risk perspective. Such projects face various risks arising from social, economic, political, workforce, and regulatory aspects during project execution. It is necessary to develop preventive strategies for managing these risks and thereby ensure timely project delivery, cost efficiency, and safety. In this study, we aimed to develop a comprehensive hybrid decision-making framework for analyzing risks in TBM-based tunnel construction projects. The proposed approach integrates the best–worst method (BWM), data envelopment analysis (DEA) model-based risk assessment, and the preference ranking organization method for enrichment evaluation (PROMETHEE). The BWM was applied to determine the weights of decision criteria with fewer comparisons and improved consistency. Subsequently, the DEA model was then used to compute local risk scores under multiple input and output conditions. Finally, PROMETHEE was employed to analyze the risks based on positive and negative outranking flows. The proposed approach was applied to a realistic metro construction project in Bangkok. The findings indicated that the proposed approach effectively compromised all the decision-making attributes to manage the uncertainties. The proposed methodology can support project managers, stakeholders, engineers, and relevant authorities in identifying high-priority risks and implementing effective mitigation strategies to enhance risk management in tunnel construction. Full article

Addressing climate change necessitates coordinated efforts across multiple sectors, with agriculture representing a significant source of greenhouse gas (GHG) emissions. This requires sophisticated mitigation strategies at the farm level. Digital decision support tools (DSTs) tailored for this purpose play a crucial role in accelerating farm-level decarbonization. Ensuring the reliability and accuracy of these DSTs mandates thorough model robustness validation. This study validates a farm-level GHG accounting and decarbonization DST using Sobol and Morris global sensitivity analyses to evaluate output robustness and to identify key input parameters critical for reliable mitigation planning. Both sensitivity analysis methods provide a comprehensive assessment of the tool’s robustness and highlight parameters most influencing farm-level GHG emission outcomes. Results show consistent outcomes across sensitivity approaches, reinforcing confidence in the tool’s application for emission reduction planning. The sensitivity analysis results indicate that the tool delivers reliable outcomes across various sensitivity analysis methods, thereby enhancing confidence in its suitability for decarbonization planning. Furthermore, the findings of this study provide a methodological foundation for future advancements and expanded use within the agriculture sector. This supports the DST’s effectiveness in prioritizing mitigation strategies and planning emission reduction pathways at the farm scale, while providing a transparent template to guide future tool improvements and broader agricultural applications. Full article

The safety of belt conveyor operation is of great importance during coal conveyance. This paper proposes a multi-task-based GSSA-YOLOM algorithm for monitoring the state of belt conveyors, which utilizes segmentation head to detect foreign objects and belt deviation, thereby balancing the trade-offs among multiple tasks. The detection neck is responsible for multi-scale feature fusion by incorporating the Asymptotic Feature Pyramid Network (AFPN) to achieve enhanced spatial perception. Then, Groupwise Separable Convolution (GSConv) is further introduced to simplify the network architecture, reducing computational complexity while maintaining sufficient detection accuracy for edge device deployment. Moreover, the SlideLoss and Soft-NMS functions are integrated to reduce the rate of false positives and missed detections. Comparison experiments were conducted, and the results indicate that the proposed GSSA-YOLOM model can improve mAP@50 by 3.4% compared with the baseline model while reducing the number of parameters by 27%, thereby satisfying coal mine safety monitoring requirements. Full article

To address the limitations of traditional rolling bearing fault diagnosis methods—such as inadequate feature extraction, limited noise robustness, and weak generalization under variable working environments—this study proposes a fault diagnosis framework that integrates parallel axial attention into a ResNet architecture. First, continuous wavelet transform (CWT), known for its inherent noise immunity, is employed to convert vibration signals into time–frequency images, providing a noise-suppressed representation of fault characteristics. Convolutional layers are then applied to reduce image dimensionality and computational complexity. A parallel axial attention module is subsequently introduced to independently capture feature dependencies along the temporal and frequency axes, enhancing the model’s ability to focus on discriminative fault-related regions while filtering out irrelevant noise. ResNet serves as the backbone network for deep feature learning and classification. Experiments on the Case Western Reserve University bearing dataset show that the proposed method achieves an average diagnostic accuracy exceeding 99.67% under multiple operating regimes. Notably, it maintains an accuracy above 95% even in high-noise environments with signal-to-noise ratios (SNRs) ranging from −4 dB to 4 dB, significantly outperforming several existing convolutional neural network-based approaches. This demonstrates the strong anti-noise capability and robustness resulting from the synergistic combination of time–frequency analysis and attention mechanisms. Furthermore, cross-dataset validation using the Southeast University bearing dataset confirms the strong generalization ability of the method. These results indicate that the proposed approach exhibits excellent diagnostic performance and practical applicability in noisy and complex industrial environments. Full article

The concentrations of nitrogen (N), phosphorus (P), potassium (K), organic matter (OM), and pH in soil are critical markers of fertility that influence crop growth and yield. Traditional wet-chemical analyses are labor-intensive, time-consuming, and costly, thereby constraining timely soil information acquisition for precision agriculture. This study evaluates whether multi-output Gaussian process regression (MOGPR) can enhance the prediction accuracy of multiple soil nutrients by exploiting their intrinsic correlations, in comparison with single-output Gaussian process regression (SOGPR). Near-infrared (NIR) spectroscopy was applied to 622 typical black soil samples collected from the Farm 855 (45°43′ N, 131°35′ E), Heilongjiang Province, China. Corresponding MOGPR and SOGPR models were developed for systematic performance comparison. Results indicated that MOGPR significantly outperformed SOGPR for nutrients exhibiting moderate-to-strong intercorrelations (N, P, K, and OM), yielding R² improvements of 0.070.28 and RPD increases of 16–40%, whereas only limited gains were observed for pH due to its weak correlations with other nutrients. These findings indicate that combining NIR spectroscopy with MOGPR offers significant potential for rapid, nondestructive assessment of multiple soil nutrients. This study further establishes a correlation-aware multi-output modeling framework that links shared spectral responses with an inter-nutrient dependency structure, providing methodological guidance for multi-nutrient soil prediction. Full article

Critical thinking (CT) is essential for navigating the complex socio-scientific issues in contemporary biomedicine. These issues cross disciplinary boundaries and involve multiple societal stakeholders. Interdisciplinary and transdisciplinary (ITD) education therefore provides a valuable context for developing CT by confronting students with diverse forms of knowledge and prompting reflection on their disciplinary assumptions. In this study, boundary crossing is used as a pedagogical framework, with a focus on identification (understanding alternative perspectives) and reflection (examining one’s own assumptions). We examine how such ITD education can foster CT by enhancing students’ appreciation of disciplinary and societal viewpoints. Data from a pre- and post-course assignment were analyzed using a convergent mixed-methods approach. Students ranked the relevance and effectiveness of sessions engaging with different perspectives and identified educational design elements that contributed to broadening their biomedical outlook. Findings indicate shifts in how students perceived the relevance of different perspectives. Particularly, appreciation of the legal perspective increased. Sessions were considered most effective when involving interaction with perspective owners, interactive learning methods, and clear instructional design. The results suggest that boundary-crossing pedagogies can support CT in higher education by engaging students in reflective engagement with different disciplinary and societal perspectives. Full article

Deep graph clustering aims to discover community structures in attributed graphs without labels and is useful for downstream applications such as citation analysis. However, existing methods often cannot make full use of both node features and graph structures, especially when the structure contains noisy links or when attributes and topology are misaligned. Our objective is to learn a robust consensus embedding for attributed graphs under such attribute–topology view misalignment in a fully self-supervised manner. To this end, we propose GDGCA (Gated Deep Graph Clustering with Contrastive Alignment), which combines a dual-stream encoder with layer-wise gated fusion and cross-view contrastive alignment, followed by DEC-style self-training to refine cluster assignments. Experiments on multiple benchmark datasets show that GDGCA achieves competitive clustering performance compared with strong baselines. We further observe stable convergence under noisy or misaligned conditions, indicating improved robustness. Overall, GDGCA provides an effective self-supervised framework for reliable deep graph clustering on real-world attributed graphs. Full article