Search Results (72)

Background and Objectives: This study aims to identify risk factors associated with MDRO infections and assess their impact on patient outcomes in Egyptian ICUs. Materials and Methods: The widespread overuse of antimicrobials has led to antibiotic multidrug resistance, posing significant challenges in intensive care units (ICUs) and leading to increased morbidity, mortality, and healthcare costs. A prospective observational study was conducted over 12 months, including 113 adult patients admitted to the ICU with confirmed bacterial infections. Comprehensive medical assessments and routine investigations were performed, including multisource cultures based on clinical suspicion. Patient histories, underlying conditions, and disease progression were documented. Patients were classified into two groups: those infected with MDROs and those without MDRO infections. Results: Significant differences were observed between patients with and without MDRO infections regarding temperature, pH, PaO₂, HCO₃, serum creatinine levels, high-dose inotropes, and inotrope dependence (p-values: 0.01, 0.028, 0.036, 0.008, <0.001, 0.013, 0.029, 0.039, <0.001, and 0.003, respectively). Additionally, cerebrovascular stroke and renal failure were significantly more frequent in MDRO-infected patients (p-values: 0.048 and 0.007, respectively). MDROs accounted for 42% of infections. The most commonly detected MDRO was Klebsiella spp. (52%). Patients with MDRO infections showed significantly higher mortality (42.6%), increased incidence of ARDS, invasive ventilation, and longer ventilation durations. Independent risk factors included prior antibiotic use (OR: 3.2; 95% CI: 1.5–6.8) and invasive device presence (OR: 2.7; 95% CI: 1.2–5.9). Conclusions: Cerebrovascular stroke and renal failure appear to be risk factors for MDRO infections. MDRO infections in ICUs are associated with poor clinical outcomes and increased complications. Improved antimicrobial stewardship and targeted prevention strategies are urgently required. Full article

Due to the significant number of microbiologically negative periprosthetic joint infections (PJIs), understanding the trend in etiology and resistance patterns is essential for the correct management of these infections. Currently, few studies have been published in Spain. In this study, we analyzed the incidence, clinical characteristics, etiology, and antibiotic resistance in patients with PJIs over the last 5 years in Navarra. In this multicentric and retrospective study, all patients diagnosed with PJIs in Navarra from 2019 to 2023 were included. Of the total 156 PJIs, 23% had negative cultures and 56% of these patients had been treated with antibiotics prior to sampling. Staphylococcus epidermidis with methicillin resistance was the predominant etiological agent, followed by Staphylococcus aureus and Cutibacterium acnes. Forty percent of the Gram-positive cocci (GPC) and 35% of the Gram-negative bacilli (GNB) were multidrug-resistant organisms (MDROs). Quinolone resistance was 46% for staphylococci and 18% for Gram-negatives. In addition, 9% of staphylococci were resistant to rifampicin. Antibiotic therapy administration prior to sampling is one of the main problems for microbiological diagnosis and is present more frequently in culture-negative PJIs (56%). New sequencing techniques could improve this difficulty. The high percentage of resistance in the microorganisms causing PJI leads us to reconsider the empirical treatment for suspected PJI, with the use of different therapeutic approaches depending on the time of infection and the possible use of new non-antibiotic therapies. Full article

Background: Antibiotic resistance (AR) is a global public health crisis, largely driven by the misuse and overuse of antibiotics. This study aimed to assess the level of knowledge, attitude, and practice (KAP) toward AR among non-medical university students in Riyadh, Saudi Arabia and identify factors that influence antibiotic use. Methods: A cross-sectional survey was conducted with 672 students from both public and private universities in Riyadh. Data were collected using a self-administered questionnaire covering sociodemographic characteristics and KAP regarding antibiotics. Results: The findings indicated that 59.1% of students had sufficient knowledge of antibiotics, while 60% had positive attitudes, and 60.6% exhibited responsible practices. However, 40.9% of students demonstrated insufficient knowledge, and factors such as age, gender, and field of study significantly impacted KAP outcomes. Females had 65.8% higher odds of demonstrating a positive attitude toward AR prevention, compared to males, and 52% higher odds of adopting appropriate practices than males. The primary source of information on antibiotics was healthcare professionals, particularly doctors. Conclusions: These findings underscore the need for targeted educational interventions to enhance awareness and promote the responsible use of antibiotics among university students, helping to mitigate the threat of antibiotic resistance. Full article

Infections caused by multidrug-resistant organisms (MDRO) are linked to poor outcomes, particularly in patients with cirrhosis. The underlying mechanisms are not fully understood and may involve a different immune response against MDRO. This study aimed to compare the in vitro immune response between multidrug-resistant (MDR) Escherichia coli and antibiotic-susceptible E. coli strains. Surface protein extract and DNA extract were obtained from MDR E. coli (n = 6) and antibiotic-susceptible E. coli (n = 6) strains isolated from infected patients with cirrhosis. The extracts were used to stimulate in vitro peripheral blood mononuclear cells from healthy donors. After 48 h, cytokine levels (IFN-γ, IL-1β, IL-10, IL-12p70, MCP-1, IL-8, IL-6, MIP-1α, and MIP-1β) were measured. We observed no significant differences in cytokine production between MDR and susceptible strains. However, we identified notable interindividual variability in cytokine production for most of the cytokines studied. Only IFN-γ and IL-6 in surface extract and MCP-1 in DNA extract showed similar levels across all donors. We conclude that the cytokine profiles induced by MDR E. coli in vitro were similar to those in susceptible strains. These findings suggest that the poor prognosis associated with MDR E. coli infections is not due to a differential immune response but rather to other factors. Full article

Background/Objectives: Data on multidrug-resistant organism (MDRO) infections in children are scarce, especially in resource-limited regions. This study aimed to estimate the prevalence of MDRO infections in pediatric intensive care units (PICUs) and characterize their epidemiologic and clinical features. Methods: A national, multicenter, point-prevalence study was conducted in 50 PICUs in Argentina over 24 h between 24 and 28 November 2023. The primary study outcome was the prevalence of ICU infections caused by MDROs. Secondary outcomes included the prevalence of carbapenemase-producing Enterobacterales (CPE) colonization, ICU mortality, and ICU length of stay (LOS_ICU). Results: 304 patients were included. The overall prevalence of infection was 45.1% (137/304); of these, 50.3% (69/137) were hospital-acquired. Among the 137 patients with reported infections, 49.6% (n = 68) were classified as definite (microbiologically confirmed) and 50.4% (n = 69) as probable (no confirmatory microbiology). Among definite infections, 20.6% (n = 14) were due to MDROs. The overall prevalence of MDRO infections was 4.6% (14/304). Extended-spectrum β-lactamase (ESBL)-producing organisms were the most commonly identified microorganisms (42.9%), followed by CPE (28.6%). Ventilator-associated pneumonia (VAP) was the most frequent location of MDRO infections. The prevalence of CPE colonization was 13.2%. Mortality was low (5.3%) and similar in patients with MDRO and non-MDRO infections. LOS_ICU was longer in patients with MDRO infections compared to patients with non-MDRO infections (81 [22–150] vs. 25 [12–27] days, respectively, p = 0.0007). Conclusions: Among 304 PICU patients, the prevalence of MDRO infections and colonization was relatively low. MDRO infections were not associated with increased mortality but were associated with longer ICU stays, compared to patients with non-MDRO infections. Full article

The growing threat of antimicrobial resistance (AMR) is a critical issue for both civilians and the military. With each successive conflict, pathogens become more resistant, making the management of infections in casualties increasingly challenging. To better understand the scope and characteristics of conflict-related AMR, a comprehensive literature search was conducted in the PubMed database in April 2025, using defined search terms related to war casualties and antimicrobial resistance. We screened and included 117 relevant publications, comprising original research articles, reviews, case series, case reports, editorials, and commentaries, published in English or French, with no date restriction. This narrative review synthesizes current evidence on multidrug-resistant bacteria most commonly isolated from war casualties, their associated resistance mechanisms, and the microbiological diagnostic tools available at various levels of the military continuum of care (Roles 1–4). It also presents strategies for preventing cross-contamination and infection in resource-limited combat settings and provides practical, field-adapted recommendations for clinicians, from first responders to specialized care providers, aiming to improve infection management in armed conflict zones and mitigate the spread of AMR. Full article

Objective: Accurate risk stratification at an early stage may reduce the incidence of infection and improve the survival rate of recipients by adopting targeted interventions. This study aimed to develop a nomogram to predict the risk of multidrug-resistant organism (MDRO) infections in liver transplant (LT) recipients. Methods: We retrospectively collected clinical data from 301 LT recipients and randomly divided them into a training set (210 cases) and validation set (91 cases) using a 7:3 split ratio. Factors related to the risk of MDRO infection after LT were determined using univariate and multivariate bidirectional stepwise logistic regression. The model’s predictive performance and discrimination ability were evaluated using receiver operating characteristic (ROC) curves, calibration curves, and decision curve analysis (DCA). Results: 56 (18.60%) patients developed a MDRO infection, including 37 (17.62%) in the training cohort and 19 (20.88%) in the validation cohort. Ultimately, five factors related to MDRO infection after LT surgery were established: ascites (OR = 3.48, 95% CI [1.33–9.14], p = 0.011), total bilirubin (OR = 1.01, 95% CI [1.01–1.01], p < 0.001), albumin (OR = 0.85, 95% CI [0.75–0.96], p = 0.010), history of preoperative ICU stay (OR = 1.09, 95% CI [1.01–1.17], p = 0.009), and length of ICU stay (OR = 3.70, 95% CI [1.39–9.84], p = 0.019). The model demonstrated strong discrimination, and the area under the curve (AUC), sensitivity, and specificity of the training set were 0.88 (95% CI [0.81–0.94]), 0.82 (95% CI [0.76–0.87]), and 0.86 (95% CI [0.75–0.98]), respectively, while for the validation set, they were 0.77 (95% CI [0.65–0.90]), 0.76 (95% CI [0.67–0.86]), and 0.68 (95% CI [0.48–0.89]). The mean absolute error (MAE) in the validation cohort was 0.029, indicating a high accuracy. DCA showed a clinical benefit within a threshold probability range of 0.1 to 0.7. Conclusions: This study developed a clinically accessible nomogram to predict the risk of MDRO infection in LT recipients, enabling early risk stratification and the real-time assessment of infection risk based on the length of postoperative ICU stay. The model incorporates five easily obtainable clinical parameters (ascites, total bilirubin, albumin, preoperative ICU stay history, and length of ICU stay) and demonstrates strong predictive performance, facilitating the early identification of high-risk patients. Future research should focus on refining the model by incorporating additional clinical factors (e.g., immunosuppressive therapy adherence) and validating its generalizability in multicenter, large-sample cohorts to enhance its clinical utility. Full article

Antibiotic resistance is a global health crisis exacerbated by the misuse of antibiotics in healthcare, agriculture, and the environment. In an intensive care unit (ICU), where high antibiotic usage, invasive procedures, and immunocompromised patients converge, resistance risks are amplified, leading to multidrug-resistant organisms (MDROs) and poor patient outcomes. The human microbiome plays a crucial role in the development and dissemination of antibiotic resistance genes (ARGs) through mechanisms like horizontal gene transfer, biofilm formation, and quorum sensing. Disruptions to the microbiome balance, or dysbiosis, further exacerbate resistance, particularly in high-risk ICU environments. This study explores microbiome interactions and antibiotic resistance in the ICU, highlighting machine learning (ML) as a transformative tool. Machine learning algorithms analyze high-dimensional microbiome data, predict resistance patterns, and identify novel therapeutic targets. By integrating genomic, microbiome, and clinical data, these models support personalized treatment strategies and enhance infection control measures. The results demonstrate the potential of machine learning to improve antibiotic stewardship and predict patient outcomes, emphasizing its utility in ICU-specific interventions. In conclusion, addressing antibiotic resistance in the ICU requires a multidisciplinary approach combining advanced computational methods, microbiome research, and clinical expertise. Enhanced surveillance, targeted interventions, and global collaboration are essential to mitigate antibiotic resistance and improve patient care. Full article

Background/Objectives: Appendicitis caused by multi-drug-resistant pathogens is associated with significant postoperative morbidity. However, prospective data on the microbial spectrum and its clinical impact remain limited. Methods: Adults with acute appendicitis undergoing surgery between April 2022 and July 2023 were prospectively enrolled at a single university-affiliated institution. Bacterial cultures from appendiceal and rectal swabs were analyzed, and clinical outcomes were assessed. A telephone follow-up was conducted 30 days postoperatively. Results: A total of 105 patients were included. Multi-drug-resistant pathogens were identified in the appendiceal swabs of twenty-nine patients (27.6%), while six patients (5.7%) harbored multi-drug-resistant organisms (MDROs; according to the criteria of the CDC). Rectal swabs revealed MDROs in 11.4% of cases but showed a limited correlation with appendiceal samples, indicating that rectal colonization does not reliably predict the presence of MDROs in appendicitis. Patients with multi-drug-resistant infections had significantly higher postoperative complication rates (31% vs. 10.5%, p = 0.017), including more Clavien–Dindo grade 3 complications (17.2% vs. 2.6%, p = 0.007) and abdominal abscesses (10.3% vs. 1.3%, p = 0.03). These patients required more frequent postoperative antibiotic treatment (65.5% vs. 40.8%, p = 0.03) and therapy adjustments (37.9% vs. 15.8%, p = 0.02). Hospital stays were also prolonged in the multi-drug-resistant group (a median of 4 days and IQR of 5 days vs. a median of 3 days and IQR of 3 days; p = 0.03). Conclusions: Colonization with multi-drug-resistant pathogens in appendicitis is associated with worse clinical outcomes. The intraoperative microbiological analysis of appendiceal swabs in complicated cases may enable targeted antibiotic therapy, potentially shortening hospital stays, optimizing patient management and reducing healthcare costs. Full article

Background/Objectives: Patients with central nervous system injuries who are hospitalized in intensive care units (ICUs) are at high risk for nosocomial infections. Limited data are available on the incidence and patterns of microbial colonization and infection in this patient population. Methods: To fill this gap, we performed an electronic health record-based study of 1614 chronic patients with brain injury admitted to the ICU from 2017 to 2023. Results: Among the infectious complications, pneumonia was the most common (n = 879; 54.46%). Sepsis was diagnosed in 54 patients, of whom 46 (85%) were diagnosed with pneumonia. The only pathogen that showed an association with the development of pneumonia and sepsis in colonized patients was Pseudomonas aeruginosa (pneumonia: p = 7.2 × 10⁻⁹; sepsis: p = 1.7 × 10⁻⁵). Bacterial isolates from patients with and without pneumonia did not differ in pathogen titer or dynamics, but patients with monomicrobial culture were more likely to develop pneumonia than patients with polymicrobial culture (1 vs. 2 pathogens, p = 0.014; 1 + 2 pathogens vs. 3 + 4 pathogens, p = 2.8 × 10⁻⁶), although the pathogen titer was lower in monoculture than in polyculture. Bacterial isolates from all patients and all culture sites showed high levels of multidrug resistance (Gram-negative bacteria: 88–100%; Gram-positive bacteria: 48–97%), with no differences in multidrug-resistant organism (MDRO) colonization and infection rates. Conclusions: Our results highlight the high burden of MDROs in neurological ICUs and provide novel ecosystem-based insights into mono- and polymicrobial colonization and infection development. These findings may be useful for developing strategies to protect against infections. Full article

Background: Healthcare facilities worldwide are struggling to combat the increasing prevalence of multidrug-resistant organisms (MDROs). MDROs pose a serious threat to patient safety, resulting in longer hospital stays, higher healthcare expenses, and increased mortality rates. Oman aims to enhance patient safety by implementing data-driven strategies for infection control protocols. MDRO studies are limited, with only one-year data available from a tertiary hospital. No comprehensive six-year study has been released from Oman, leaving a gap in understanding the true burden of MDROs in the country. Methods: A 6-year retrospective cross-sectional analysis of MDRO files at Ibri Regional Hospital was conducted from 1 January 2017 to 31 December 2022 on clinical samples (urine, pus, eye, ear, tissue, fluid, wound, CSF, blood, ET, and sputum culture). Non-clinical and duplicate samples were excluded. Two prevalence rates were calculated: isolates and patients per 1000 admissions. Isolates were identified using the API system, VITEK-2, and MALDI-TOF MS. CLSI guidelines that are updated annually were used for antibiotic susceptibility testing. Statistical analysis was performed using SPSS to identify significant associations between pre-established variables. Ethical approval was obtained from the Centre for Studies and Research (MOH/CSR/26193). Results: MDRO prevalence in our cohort (1846 cases) was illustrated per isolate and per patient (1000 admission) and showed a growing trend; 25.60:18.70/1000 (2017), 24.40:22.40/1000 (2018), 26.80:19.40/1000 (2019), 31.20:21.70/1000 (2020), 32.50:19.00/1000 (2021), and 31.70:20.40/1000 (2022). Urinary infections were predominant, followed by surgical, respiratory, blood, and neurological infections. Respiratory infections increased during the COVID-19 era. ESBL remained the primary burden, followed by Acinetobacter MDR, MRSA, CRE, pseudomonas MDR, and VRE. VRE showed a spike in 2021 but returned to baseline in 2022. Conclusions: The study reveals a growing trend of MDROs in Oman, with species prevalence similar to that of adjacent countries. Urgent actions needed include national antibiotic policies, a local antibiogram database, public awareness campaigns on antibiotic resistance, and predicting bed occupancy for expansion/construction programs. Full article

Background: Healthcare-acquired infections due to multidrug-resistant organisms (MDR-HAIs) pose globally significant challenges to healthcare systems, leading to increased morbidity, mortality, and healthcare costs. According to the World Health Organization, the COVID-19 pandemic significantly impacted the burden of MDR-HAIs. The aim of this study was to investigate the dynamics and epidemiology of MDR-HAIs in inpatients at the University of Leipzig Medical Center (ULMC) before and during the COVID-19 pandemic. Methods: We compared data from inpatients with bacterial infections from 2017 to 2019 (pre-COVID-19) and from 2021to 2023 (during COVID-19) in a cross-sectional, monocentric, retrospective survey. This study focused on selected multidrug-resistant organisms (MDROs) and four clinical specimens. We analyzed the risk factors for MDR-HAIs using logistic regression models. Results: Out of 342,705 inpatients, 32,206 were diagnosed with a bacterial infection. The prevalence increased significantly from 8.09% (pre-COVID-19) to 10.79% (during COVID-19) (p < 0.001), but the proportion of MDROs decreased from 10.14% to 8.07%. The proportions of MDR-HAIs were 59.86% and 56.67%, respectively. The proportion of carbapenem-resistant Klebsiella pneumoniae and Enterobacter cloacae increased significantly. The risk of MDR-HAIs during COVID-19 decreased by 18% compared to pre-COVID-19 (p = 0.047). Longer hospital stays increased the risk of MDR-HAIs in both periods. This risk significantly decreased for children and the elderly during COVID-19. Conclusion: These findings show that it is also important to analyze epidemiological data at the facility level in order to evaluate the effectiveness of infection control practices even during unprecedented health crises like the COVID-19 pandemic. Full article

Healthcare-associated infections (HAIs) significantly increase morbidity, mortality, length of hospital stays, and costs, particularly among ICU patients. Despite standard interventions, catheter-associated urinary tract infections (CAUTI) and central line-associated bloodstream infections (CLABSI) remain major HAI contributors. This study evaluated the efficacy of daily 2% chlorhexidine gluconate (CHG) bathing in reducing HAI incidence, specifically CAUTI, CLABSI, and multidrug-resistant organisms (MDROs), in a 20-bed ICU at a regional hospital. Using a prospective, uncontrolled before-and-after design, we compared traditional soap-water bathing (pre-intervention period) with CHG bathing over a one-year intervention and one-year post-intervention follow-up. The total number of patients and patient days admitted to the ICU per year were around 1330–1412 patients and 6702–6927 patient days, respectively, during 2018–2020. Results showed a significant reduction in HAI incidence rates from 3.43‰ to 0.58‰ (p < 0.05) during the intervention and sustained benefits post-intervention. Incidences of CAUTI and CLABSI decreased markedly (p < 0.05), with reduced MDRO isolates, including methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococci, carbapenem-resistant Acinetobacter baumannii, and Pseudomonas aeruginosa. Our findings support the implementation of daily CHG bathing as an effective strategy to reduce HAI and MDROs in ICU settings. Full article

In solid organs post-transplant, bacterial infections can complicate the course of recovery with devastating consequences, such as graft loss and death. We provide an expert review on early post-liver transplant bacterial infections, with a focus on infections with multi-drug-resistant organism (MDRO) etiologies. Best practice recommendations are derived from a combination of available evidence and expert consensus. The main challenge in managing antibiotic therapy arises in patients with severe clinical conditions but negative MDRO screening results, as well as in those with positive MDRO screening results but uncomplicated infections. With the aim of shedding light on these “gray areas”, we propose an algorithm where the patient is stratified as being at low risk or high risk of developing an MDRO infection. Full article

Background: Lactiplantibacillus plantarum can inhibit the growth of multidrug-resistant organisms (MDROs) and modulate the gut microbiome. However, data on hematopoietic stem cell transplantation (HSCT) are scarce. Aim: In an observational study, we assessed the impact of L. plantarum on the modulation of the gut microbiome in HSCT patients colonized by MDROs. Methods: Participants were allocated to an intervention group (IG = 22) who received capsules of L. plantarum (5 × 10⁹ CFU) twice per day until the onset of neutropenia or a control group (CG = 20). The V4 region of the 16S bacterial rRNA gene was sequenced in 87 stool samples from a subset of 33 patients (IG = 20 and CG = 13). The Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2) program was used to predict metagenome functions. Results: L. plantarum demonstrated an average 86% (±11%) drug-target engagement at 43 (±29) days of consumption and was deemed safe, well-tolerated, and associated with an increase in the abundance of the Lactobacillales (p < 0.05). A significant increase in Lactococcus and a reduction in Turicibacter (p < 0.05) were identified on the second week of L. plantarum use. Although Enterococcus abundance had a greater rise in the CG (p = 0.07), there were no significant differences concerning the Gram-negative MDROs. No serious adverse effects were reported in the IG. We observed a greater, non-significant pyruvate fermentation to propanoate I (p = 0.193) relative abundance in the IG compared with the CG. L. plantarum use was safe and tolerable by HSCT patients. Conclusions: While L. plantarum is safe and may impact Enterococcus and Turicibacter abundance, it showed no impact on Gram-negative MDRO abundance in HSCT patients. Full article