Search Results (112)

Biomimetic design, derived from the study of biological systems, has emerged as a pivotal methodology in contemporary art and design. By systematically integrating the morphological traits, structural principles, and functional mechanisms of living organisms into design thinking, it provides both a novel theoretical perspective and methodological support for modern design practice. This design philosophy draws abundant inspiration from nature’s aesthetics and achieves a profound fusion of organic form and artistic expression. This study systematically traces the theoretical evolution of biomimetic design—from its early phase of direct form-mimicry to today’s holistic, systems-based approach—and clarifies its interdisciplinary logic and developmental trajectory. We examine its applications in public installations, product development, architecture, and fashion. Through a structured analysis of plant-inspired, animal-inspired, and ecosystem-inspired strategies—linked with the aesthetic demands and cultural contexts of design—this study uncovers the underlying mechanisms by which biological models drive innovation. The findings demonstrate that, by organically combining form simulation, function optimization, and ecological awareness, biomimetic design not only elevates the aesthetic value, visual impact, and emotional resonance of design works but also amplifies their social role and cultural significance. Moreover, its interdisciplinary potential in materials innovation, technological integration, and environmental sustainability highlights unique pathways for addressing complex contemporary challenges. This study adopts a methodology that blends case-study analysis and theoretical interpretation. Through an in-depth examination of exemplar projects, it validates that biomimetic design not only achieves a seamless unity of function and form but also offers a robust theoretical framework and practical strategies for sustainable design implementation. These insights advance both the theoretical depth and practical innovation of the design discipline. Full article

Verticillium-like fungi within the Sordariomycetes hold significant ecological and economic importance, especially in biocontrol. This study describes two novel species, Leptobacillium gasaense and Ovicillium yunnanense, and provides DNA sequence data and identification keys for the genera Leptobacillium and Ovicillium. The genus Muscodor, known for its considerable biotechnological value, comprises endophytes characterized by sterile mycelia that produce antibiotic volatile organic compounds (VOCs). Historically, the classification of Muscodor has relied on culture characteristics, VOC chemical profiles, and molecular phylogenetic analyses. However, culture characteristics and VOC profiles lack a definitive diagnostic value. Although asexual morphological traits are crucial for genus-level classification, no conidiogenous structures have been observed in Muscodor. Here, we report the asexual morphological characteristics of Muscodor and describe M. coffeanus as a new record in China, supported by both its asexual morphology and molecular phylogenetic evidence. Full article

This study investigates the potential role of Acrocalymma dark septate endophytic (DSE) fungi in promoting the growth of Solanum lycopersicum (tomato). Recognized as important symbionts that enhance plant growth and resilience under stress, particularly Acrocalymma species, DSE fungi were the focus of this investigation. Specifically, four stains isolated from gramineous plant roots (Acrocalymma sp. E00677, Acrocalymma vagum E00690, Acrocalymma chuxiongense E01299A, and Acrocalymma chuxiongense E01299B) were examined. Morphological characteristics were observed using three different media, confirming typical DSE traits such as dark pigmentation and septate hyphae. Phylogenetic analysis using six genetic markers (ITS, LSU, SSU, tef1, rpb2, and tub2) placed the strains within the Acrocalymma genus. Co-culture test and physiological index measurements showed that all strains significantly enhanced root development, as evidenced by an increased root-to-shoot ratio and a higher number of lateral roots. Additionally, the Acrocalymma DSE strains elevated chlorophyll a, chlorophyll b, and total chlorophyll content, suggesting improved photosynthetic efficiency. Anthocyanin levels were also increased in the tomato leaves, indicating enhanced antioxidative defense mechanisms. Among these strains, Acrocalymma vagum E00690 exhibited the most substantial effect on root activity. The widespread presence of 325 Acrocalymma isolates from 25 countries underscores its broad ecological adaptability. These findings suggest that Acrocalymma DSE fungi positively influence tomato growth, with potential implications for improving plant resilience under environmental stress. This study highlights the importance of further exploring DSEs, particularly Acrocalymma fungi, to better understand their ecological roles in agricultural practices, particularly in tomato cultivation. Full article

The large yellow croaker (Larimichthys crocea) is one of China’s most economically important marine fish species, with its cage culture production leading the nation for many years. However, the rapid expansion of aquaculture has brought challenges such as germplasm degradation, reduced disease resistance, inconsistent product quality, and low adoption of improved strains, which have hindered the sustainable development of the industry. The primary objective of this review is to summarize the current practices and challenges in seedling selection for L. crocea. The secondary objectives include discussing the influence of genetic, physiological, and environmental factors on growth performance and proposing future research directions for sustainable breeding programs. This review covers key topics including morphological screening, growth performance evaluation, genetic diversity conservation, disease resistance improvement, and adaptation to environmental stress. It also explores the application of modern technologies such as marker-assisted selection, intelligent monitoring, environmental control, precision feeding, and disease prevention. Moreover, it highlights core issues in current breeding practices, such as over-reliance on single-trait selection and insufficient integration of environmental adaptability and disease resistance. Finally, future trends are discussed, emphasizing the integration of genomic tools with artificial intelligence to promote intelligent, precise, and sustainable breeding approaches. These insights aim to enhance aquaculture productivity while supporting long-term ecological balance and industry sustainability. Full article

Aquaculture has become increasingly important as a source of high-quality animal protein and fatty acids for humans. This study investigated the morphological traits, general nutritional component, amino acid composition, fatty acid composition, and antioxidant enzyme activity of artificially cultured whitespotted conger Conger myriaster from three different suppliers, Haiyang Yellow Sea Fisheries Co., Ltd. (YT), Rizhao Rongwang aquatic science and technology Co., Ltd. (RZ), and Weihai Shenghang aquatic science and technology Co., Ltd. (WH), based on the Yellow Sea coast in China. Of the 19 morphological traits, total length, body length, and vertical eye diameter were significantly different in the C. myriaster of YT, RZ, and WH (p < 0.05). The hepatosomatic index (HSI) of YT was significantly lower than that of RZ and WH (p < 0.05), and the gonadosomatic index (GSI) was not significantly different. The moisture, crude lipid, and crude ash contents in YT were significantly different from those in RZ and WH (p < 0.05). The methionine (Met) of RZ was significantly higher than that of YT and WH (p < 0.05). C. myriaster from YT, RZ, and WH were detected to have 26, 27, and 26 fatty acids, respectively. The docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) contents of YT were significantly higher than those of RZ and WH (p < 0.05). There were no significant differences in the antioxidant enzyme activities of C. myriaster from YT, RZ and WH (p > 0.05). The results showed that there are differences in the morphological traits, general nutritional component proximate composition, and amino acid and fatty acid compositions of cultured C. myriaster from different regions. Full article

The low availability of phosphorus (P) in soil has become a critical factor limiting crop growth and agricultural productivity. This study aimed to isolate and evaluate a bacterial strain with high phosphate-solubilizing capacity to improve soil phosphorus utilization and promote crop growth. A phosphate-solubilizing bacterium, designated as YS-13, was isolated from farmland soil in Henan Province, China, and identified as Brevibacillus laterosporus based on morphological characteristics, physiological and biochemical traits, and 16S rDNA sequence analysis. Qualitative assessment using plate assays showed that strain YS-13 formed a prominent phosphate solubilization zone on organic and inorganic phosphorus media containing lecithin and calcium phosphate, with D/d ratios of 2.28 and 1.57, respectively. Quantitative evaluation using the molybdenum–antimony colorimetric method revealed soluble phosphorus concentrations of 21.24, 6.67, 11.73, and 17.05 mg·L⁻¹ when lecithin, ferric phosphate, calcium phosphate, and calcium phytate were used as phosphorus sources, respectively. The fermentation conditions for YS-13 were optimized through single-factor experiments combined with response surface methodology, using viable cell count as the response variable. The optimal conditions were determined as 34 °C, 8% inoculum volume, initial pH of 7.55, 48 h incubation, 5 g L⁻¹ NaCl, 8.96 g L⁻¹ glucose, and 8.86 g L⁻¹ peptone, under which the viable cell count reached 6.29 × 10⁸ CFU mL⁻¹, consistent with the predicted value (98.33%, p < 0.05). The plant growth-promoting effect of YS-13 was further validated through a pot experiment using Triticum aestivum cv. Jinchun 6. Growth parameters, including plant height, fresh biomass, root length, root surface area, root volume, and phosphorus content in roots and stems, were measured. The results demonstrated that YS-13 significantly enhanced wheat growth, with a positive correlation between bacterial concentration and growth indicators, although the growth-promoting effect plateaued at higher concentrations. This study successfully identified a high-efficiency phosphate-solubilizing strain, YS-13, and established optimal culture conditions and bioassay validation, laying a foundation for its potential application as a microbial inoculant and providing theoretical and technical support for reducing phosphorus fertilizer inputs and advancing sustainable agriculture. Full article

Zingiberaceae is a diverse and culturally significant plant family across tropical Asia, yet in Roi Et Province, Thailand, it has remained poorly documented. This study aimed to provide the first comprehensive assessment of Zingiberaceae in Roi Et Province, Thailand. Previous studies in the region have been limited and did not systematically document species occurrence. The assessment began with an overview of species diversity, followed by an investigation of their ethnobotanical uses, an evaluation of their horticultural potential, and an assessment of their conservation status. Field surveys were conducted throughout Roi Et Province across various habitat types using exploratory sampling to record all observable Zingiberaceae species. Species were identified based on morphological characteristics and comparisons with existing taxonomic literature. A total of 97 species were recorded, including 86 native and 11 introduced taxa, with 23 species endemic to Thailand and 25 species featuring new distributions reported for the province. Species richness was highest in cultivated habitats, likely reflecting the active role of traditional agroecosystems in conserving both native and introduced taxa. Natural habitats such as wetlands and dry evergreen forests hosted species with restricted distributions, underscoring the need to preserve ecological heterogeneity. Ethnobotanical interviews revealed widespread local use of rhizomes and inflorescences for food, medicine, ornamentation, and ceremonial purposes. Phenological observations indicated synchronized flowering and fruiting aligned with the monsoon season, which are traits that supports successful reproduction but may also increase vulnerability under shifting climate regimes—highlighting the importance of monitoring for conservation and guiding optimal harvest timing. These findings demonstrate that both natural and cultivated systems are integral to maintaining Zingiberaceae diversity in the region. Conservation strategies should prioritize habitat protection, support traditional cultivation practices, and promote further research into economically and culturally valuable species. Full article

To address the growing consumer demands for improved fish meat quality, desirable morphological traits, and sustainable production practices, researchers have intensified efforts in the selective breeding and genetic improvement of carp (Cyprinus carpio) varieties. However, traditional breeding methods are often time-consuming and inefficient, which poses challenges to the sustainable development of the carp aquaculture industry. The establishment of germ stem cell lines offers a crucial tool for the study of germ cells, genetic improvement, and species conservation. In this study, we successfully established a spermatogonial stem cell line (YRSSCs) from Yellow River carp (Cyprinus carpio haematopterus) that can be cultured in vitro for the long term. We optimized the culture conditions to maintain their self-renewal and differentiation capabilities. The results demonstrated that YRSSCs have a diploid karyotype and can stably proliferate for over a year in L-15 medium supplemented with 5 mmol/L HEPES, 50 μmol/L β-mercaptoethanol, 15% FBS, 2 ng/mL bFGF, 2 ng/mL LIF, 1% carp serum, 800 IU/mL penicillin, 0.8 mg/mL streptomycin, 2 μg/mL amphotericin B, 1% zebrafish embryo extract, and 1% glutamine at 30 °C in the absence of CO₂. The cells exhibited a typical germ stem cell gene expression profile, with strong expression of the vasa, plzf-a, and Oct4-a genes. Additionally, this study found that YRSSCs possess the ability to differentiate in vitro and functionally colonize in vivo within recipient bodies. This research explored the establishment of YRSSCs and their differentiation potential both in vitro and in vivo, providing a novel strategy for the genetic improvement of aquaculture fish species through germ stem cell-based gene editing and transplantation technologies. Full article

The genus Taxus comprises a unique group of gymnosperms known for their botanical longevity, cultural significance, and exceptional pharmacological potential. This review explores the multifaceted profile of Taxus species, with a focus on their morphological traits, phytochemical composition, traditional uses, and therapeutic applications. Particular attention is given to taxanes, especially paclitaxel, which have revolutionized cancer treatment through microtubule-stabilizing mechanisms. In addition to well-established uses of the bark and leaves, the review synthesizes emerging research on the aril, a non-toxic and antioxidant-rich plant part, suggesting novel biomedical applications. By integrating ethnobotanical knowledge with contemporary pharmacological insights, this work underscores the enduring relevance of Taxus in traditional medicine while emphasizing its evolving role in modern drug discovery. The findings advocate for intensified interdisciplinary research and sustainable exploitation strategies to fully harness the genus’s therapeutic potential without compromising biodiversity. Full article

Garlic (Allium sativum L.) is the second most significant species within the Allium genus worldwide, widely used in cooking and both traditional and modern medicine due to its beneficial biological and therapeutic properties. In Italy, diverse pedo-climatic conditions and historical–cultural fragmentation have led to the development of various garlic landraces, prized for their unique organoleptic qualities and cultural importance. This study aimed to assess the intra-varietal diversity and uniqueness of two red garlic landraces from the Lazio region in central Italy, “Aglio Rosso di Castelliri” and “Aglio Rosso di Proceno”, using SSR and ISSR molecular markers, along with evaluations of bulb morphological traits, total phenolic content, and antioxidant properties. The molecular analysis included 11 accessions of “Aglio Rosso di Castelliri”, nine of “Aglio Rosso di Proceno”, and 15 control accessions, comprising eight Italian red-type garlic landraces, four Spanish red garlic commercial varieties, two white garlic accessions, and an accession of A. ampeloprasum var. holmense used as an outgroup. SSR and ISSR markers revealed moderate genetic diversity within the collection, with mean PIC values of 0.41 and 0.17, respectively. The molecular data identified four distinct genetic clusters, with the two Lazio landraces forming separate groups, indicating their genetic distinctiveness. The results from the STRUCTURE analysis support the hypothesis that these landraces may have originated from the widely cultivated “Aglio Rosso di Sulmona” or a common ancestral population once prevalent in central Italy. The study also revealed significant intra-population genetic diversity within the two garlic landraces, underscoring the need for in situ conservation and clonal selection. Phenotypic evaluations confirmed the distinctiveness of the two landraces, with “Aglio Rosso di Castelliri” characterized by smaller bulbs and cloves with higher dry matter content and distinct color profiles. Additionally, significant variation in total phenolic content and antioxidant activity was observed by analyzing 13 selected accessions from the two landraces (six from “Aglio Rosso di Proceno” and seven from “Aglio Rosso di Castelliri”) and five red garlic control accessions, with the two Lazio landraces exhibiting higher levels than the control group. This study highlights the importance of integrating molecular, phenotypic, and chemical analyses to understand garlic landrace diversity, with significant implications for their conservation and protection of local agro-food products. Full article

In vitro anther culture is a technique used to produce haploid plants when regenerating varieties with specific traits. To generate haploid plants with preferred characteristics, an anther culture technique was established for Lilium longiflorum “Show Up”. Morphological characteristics were recorded, including the flower bud length and anther color corresponding to different stages of microspore development. The effects of different flower bud lengths, various concentrations of exogenous plant growth regulators (PGRs), low-temperature pretreatment at 4 °C, and incubation under dark conditions on the induction of callus formation were studied. When the flower buds were 2.2–2.4 cm in length and the microspores were in the mononuclear development phase, callus induction reached the highest rate (15.6%). Callus was not induced when the PGRs 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KT) were added separately to the growth medium, but the highest callus induction rate occurred when anthers were cultured on the medium containing 2,4-D (0.75–1.0 mg/L) and KT (4 mg/L). The low-temperature pretreatment significantly enhanced the induction rate of anthers, but prolonged low-temperature pretreatment reduced the induction rate. The optimal period of cultivation in darkness was 6 d. After 15 days of cultivation, the number of swollen anthers was recorded, and these were transferred onto the differentiation medium Murashige and Skoog (MS) + 1-naphthaleneacetic acid (NAA) (2.0 mg/L), sucrose (30 g/L), and agar (7 g/L) at pH 5.8, whereon 100% differentiation was recorded. Overall, 14 regenerated lines were obtained by in vitro anther culture. Chromosome ploidy was determined by counting chromosomes in the root tips of ten regenerated plants, and four were found to be haploids. This study lays the foundation for anther culture in lilies to shorten the breeding cycle, improve selection efficiency, facilitate efficient genetic transformation, and enable the effective production of both haploid and double-haploid plants. Full article

Carob (Ceratonia siliqua L. Fabaceae) is a species of significant economic, ecological, and cultural importance in the Mediterranean region. It is valued for its adaptability to various environments and wide-ranging agricultural and industrial applications. Despite its potential, the genetic characterization of this species in Algerian territories has received little attention to date. The present study aims to decipher carob’s genetic structure and diversity in Algeria. This study presents a comprehensive morphological characterization of 39 Algerian carob accessions based on pod traits and molecular genotyping using eight nuclear and three chloroplast SSR markers across different geographical locations and environmental conditions. The morphological analysis revealed a discrete differentiation among accessions, primarily influenced by their area of origin. The genetic analysis identified 38 unique genotypes. Allelic richness indicated high polymorphism, with an average of 3.81 alleles and 5.36 genotypes for nuclear SSR markers. Chloroplast SSR markers showed lower variability but provided insights into population structure. Genetic analysis revealed distinct genetic clusters aligned with geographical and bioclimatic regions, supported by minimum spanning network analysis that showed the genetic flow patterns among accessions. Discriminant analysis of principal components identified five optimal sub-population groups, highlighting a genetic structure linked with different bioclimatic conditions. These findings evidence the complex genetic diversity of Algerian carob germplasm and offer valuable insights for the sustainable exploitation of carob genetic resources. Full article

The largescale blackfish, Girella punctata Gray, 1835, is important in the fishing industry and recreational fishing, and it is also cultured in East Asia. Cymothoidae (Crustacea: Isopoda) is a group of parasites that infest fish in marine, brackish, and freshwater environments. In this study, we report, for the first time, Ceratothoa arimae (Nunomura, 2001) (Cymothoidae) from the buccal cavity of G. punctata in the Seto Inland Sea, Japan. Ceratothoa arimae showed a prevalence of 29.4–66.7% in G. punctata. The morphology of the mancae of this species was also described in comparison with that of the adult female (ovigerous), transitional stage, and adult male. The manca of Ceratothoa arimae has more chromatophores than those of other Ceratothoa species from Japan, and is a candidate for a future taxonomic trait. This species may have a negative impact on cultured G. punctata, which would be important to determine in future studies. Currently, it is difficult to identify cymothoid mancae species based on their morphology, but the information provided in this study could be useful when combined with other methods developed in the future, such as molecular analysis. Full article

Hyperhydricity is characterized by morphological abnormalities and reduced plant vigour. This study investigated the use of a bottom cooling system (creating an approximate 2 °C temperature differential) during culture initiation to evaluate the impact on hyperhydricity in cannabis micropropagation. Nodal explants from two clonal triploid cultivars known to exhibit hyperhydricity, Higher Education 1 (HED-1) and Higher Education 2 (HED-2), were surface sterilized and placed in culture tubes using standard methods. Treatments included bottom cooling, metal pads without bottom cooling, and standard shelving (controls—no pad). Various morphological and physiological traits were assessed, including a detached leave water loss assay, dry mass, chlorophyll content, and survival rate. Plants cultured with bottom cooling showed significantly higher survival rates, healthier appearance, and improved physiological parameters compared to controls. In contrast, many control explants were hyperhydric with translucent and brittle leaves. Quantitative data revealed significant improvements in fresh weight (54.84% for HED-1 and 51.42% for HED-2), dry weight (36% for HED-1 and 8% for HED-2), chlorophyll fluorescence ratios (7.24% for HED-1 and 9.18% for HED-2), chlorophyll content (18.38% for HED-1 and 20.67% for HED-2), and cuticle/stomate function (30% for HED-1 and 27.27% for HED-2) using bottom cooling. Moreover, our morphological observation showed that almost 85% of control plants were hyperhydric, whereas only 10% of the plants cultured with a bottom cooling system were hyperhydric. This study confirmed that bottom cooling helps reduce the rate and impacts of hyperhydricity in cannabis and significantly improves the survival and quality of in vitro plants. Full article

Muscadine grapes are renowned for their unique traits, natural disease resistance, and rich bioactive compounds. Despite extensive research on their phytochemical properties, microbial communities, particularly endophytic bacteria, remain largely unexplored. These bacteria play crucial roles in plant health, stress tolerance, and ecological interactions. This study represents the first comprehensive effort to isolate, identify, and functionally characterize the bacterial endophytes inhabiting muscadine grape berries using a culture-dependent approach. We isolated diverse bacterial species spanning six genera—Bacillus, Staphylococcus, Paenibacillus, Calidifontibacillus, Curtobacterium, and Tatumella. Microscopic and physiological analysis revealed variations in bacterial morphology, with isolates demonstrating adaptability to varied temperatures. Cluster-based analysis indicated functional specialization among the isolates, with species from Pseudomonadota and Actinomycetota exhibiting superior plant growth-promoting abilities, whereas Bacillota species displayed potential biocontrol and probiotic properties. Among them, Tatumella ptyseos demonstrated exceptional plant growth-promoting traits, including indole-3-acetic acid production, nitrogen fixation, phosphate solubilization, and carbohydrate fermentation. Additionally, Bacillus spp. showed presumptive biocontrol potential, while Paenibacillus cineris emerged as a potential probiotic candidate. The identification of Calidifontibacillus erzurumensis as a novel endophytic species further expands the known biodiversity of grape-associated microbes. These findings provide insights into the metabolic diversity and functional roles of muscadine grape-associated endophytes, highlighting their potential for agricultural and biotechnological applications. Full article