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Keywords = microfracture activity

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26 pages, 5958 KB  
Article
A Material–Structure Integrated Approach for Soft Rock Roadway Support: From Microscopic Modification to Macroscopic Stability
by Sen Yang, Yang Xu, Feng Guo, Zhe Xiang and Hui Zhao
Processes 2026, 14(3), 414; https://doi.org/10.3390/pr14030414 - 24 Jan 2026
Viewed by 325
Abstract
As a cornerstone of China’s energy infrastructure, the coal mining industry relies heavily on the stability of its underground roadways, where the support of soft rock formations presents a critical and persistent technological challenge. This challenge arises primarily from the high content of [...] Read more.
As a cornerstone of China’s energy infrastructure, the coal mining industry relies heavily on the stability of its underground roadways, where the support of soft rock formations presents a critical and persistent technological challenge. This challenge arises primarily from the high content of expansive clay minerals and well-developed micro-fractures within soft rock, which collectively undermine the effectiveness of conventional support methods. To address the soft rock control problem in China’s Longdong Mining Area, an integrated material–structure control approach is developed and validated in this study. Based on the engineering context of the 3205 material gateway in Xin’an Coal Mine, the research employs a combined methodology of micro-mesoscopic characterization (SEM, XRD), theoretical analysis, and field testing. The results identify the intrinsic instability mechanism, which stems from micron-scale fractures (0.89–20.41 μm) and a high clay mineral content (kaolinite and illite totaling 58.1%) that promote water infiltration, swelling, and strength degradation. In response, a novel synergistic technology was developed, featuring a high-performance grouting material modified with redispersible latex powder and a tiered thick anchoring system. This technology achieves microscale fracture sealing and self-stress cementation while constructing a continuous macroscopic load-bearing structure. Field verification confirms its superior performance: roof subsidence and rib convergence in the test section were reduced to approximately 10 mm and 52 mm, respectively, with grouting effectively sealing fractures to depths of 1.71–3.92 m, as validated by multi-parameter monitoring. By integrating microscale material modification with macroscale structural optimization, this study provides a systematic and replicable solution for enhancing the stability of soft rock roadways under demanding geo-environmental conditions. Soft rock roadways, due to their characteristics of being rich in expansive clay minerals and having well-developed microfractures, make traditional support difficult to ensure roadway stability, so there is an urgent need to develop new active control technologies. This paper takes the 3205 Material Drift in Xin’an Coal Mine as the engineering background and adopts an integrated method combining micro-mesoscopic experiments, theoretical analysis, and field tests. The soft rock instability mechanism is revealed through micro-mesoscopic experiments; a high-performance grouting material added with redispersible latex powder is developed, and a “material–structure” synergistic tiered thick anchoring reinforced load-bearing technology is proposed; the technical effectiveness is verified through roadway surface displacement monitoring, anchor cable axial force monitoring, and borehole televiewer. The study found that micron-scale fractures of 0.89–20.41 μm develop inside the soft rock, and the total content of kaolinite and illite reaches 58.1%, which is the intrinsic root cause of macroscopic instability. In the test area of the new support scheme, the roof subsidence is about 10 mm and the rib convergence is about 52 mm, which are significantly reduced compared with traditional support; grouting effectively seals rock mass fractures in the range of 1.71–3.92 m. This synergistic control technology achieves systematic control from micro-mesoscopic improvement to macroscopic stability by actively modifying the surrounding rock and optimizing the support structure, significantly improving the stability of soft rock roadways. Full article
(This article belongs to the Section Petroleum and Low-Carbon Energy Process Engineering)
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21 pages, 12162 KB  
Article
Thermal Displacement with CO2 for E-CBM Recovery: Mechanisms and Efficacy of Temperature–Pressure Synergy in Permeability Enhancement
by Xiaohu Xu, Tengze Ge, Ersi Gao, Shuguang Li, Kai Wei, Yulong Liu and Ao Wang
Energies 2026, 19(2), 496; https://doi.org/10.3390/en19020496 - 19 Jan 2026
Viewed by 240
Abstract
The efficient development of coalbed methane (CBM) faces persistent challenges due to low recovery rates. While CO2 thermal displacement offers a promising approach, the pore–fracture structure (PFC) evolution and gas displacement mechanisms under temperature–pressure coupling remain insufficiently clear. To address this knowledge [...] Read more.
The efficient development of coalbed methane (CBM) faces persistent challenges due to low recovery rates. While CO2 thermal displacement offers a promising approach, the pore–fracture structure (PFC) evolution and gas displacement mechanisms under temperature–pressure coupling remain insufficiently clear. To address this knowledge gap, the in situ, dynamic quantification of pore–fracture evolution during CO2 displacement was achieved by an integrated system with NMR and CT scanning, revealing the expansion, connection, and reconfiguration of coal PFC under temperature–pressure synergy and establishing the intrinsic relationship between supercritical CO2 (ScCO2)-induced permeability enhancement and methane displacement efficiency. Experimental results identify an observed transition in permeability near 80 °C under the tested conditions as a critical permeability transition point: below this value, permeability declines from 0.61 mD to 0.49 mD, reflecting pore structure adjustment; above it, permeability rises markedly to 1.18 mD, indicating a structural shift toward fracture-dominated flow. A “pressure-dominated, temperature-assisted” mechanism is elucidated, wherein pressure acts as the primary driver in creating macro-fractures and forming percolation pathways, while temperature—mainly via thermal stress—promotes micro-fracture development and assists gas desorption, offering only limited direct contribution to permeability. Although elevated injection pressure enhances permeability and establishes fracture networks, displacement efficiency eventually reaches a physical limit. To transcend this constraint, a synergistic production mechanism is proposed in which pressure builds flow channels while temperature activates microporous desorption. This study provides an integrated, in situ quantification of the pore–fraction evolution under high-temperature ScCO2 conditions. The elucidated synergy between pressure and temperature offers insights and an experimental basis for the design of deep CBM recovery and CO2 storage strategies. Full article
(This article belongs to the Special Issue Advances in Unconventional Reservoirs and Enhanced Oil Recovery)
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18 pages, 3639 KB  
Article
Macroscopic and Microscopic Investigation on Microfractures in Blast-Conditioned Rock and the Influence of Particle Size
by Jacopo Seccatore, Sebastian Flores, Jose Oliden, Guillermo Pozo and Tatiane Marin
Appl. Sci. 2026, 16(2), 655; https://doi.org/10.3390/app16020655 - 8 Jan 2026
Viewed by 308
Abstract
In the mining industry, particle size reduction is the most energy-demanding activity. Blasting represents the first stage of comminution. Experimental and field observations have demonstrated that blasting produces two main effects on rock: (i) macroscopic fracturing and fragmentation, and (ii) microscopic fracturing, consisting [...] Read more.
In the mining industry, particle size reduction is the most energy-demanding activity. Blasting represents the first stage of comminution. Experimental and field observations have demonstrated that blasting produces two main effects on rock: (i) macroscopic fracturing and fragmentation, and (ii) microscopic fracturing, consisting of a network of microfractures that weaken the rock, reduce the specific Work Index, and make the material less resistant to crushing and milling. The present work represents an initial investigation into the relationship between blast-induced microfracturing, fragment size, and mechanical resistance. Blasted rock was analyzed using three approaches: macroscopic testing via point load tests, laboratory grinding tests using a Bond ball mill to determine the blasted Work Index, and microscopic optical observation of microfractures. The results show that macroscopic testing is unable to detect microscopic weakening, as no correlation was observed between point load strength and particle size. In contrast, laboratory ball mill tests and microscopic optical observations indicate a preliminary relationship between particle size and the internal weakening of particles. These results allow the formulation of a new hypothesis: that the Work Index may not be constant within a given volume of blasted rock and could depend on the particle size distribution. Full article
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25 pages, 14687 KB  
Article
Tectonic Control on Mineralogical and Microfabric Modifications and Their Impact on Rock Strength: Evidence from the Fault Damage Zone, Tethyan Himalayas, Pakistan
by Izhar Ahmed, Yanjun Shang, Luis Sousa, Xuetao Yi, Qingsen Meng, Hussain Rabbani and Rehan Ahmed
Minerals 2025, 15(12), 1272; https://doi.org/10.3390/min15121272 - 30 Nov 2025
Viewed by 605
Abstract
This study examines the influence of tectonically induced mineralogical and microfabric changes on the strength of different rocks within the Hanzel Fault Damage Zone (FDZ) in the Tethyan Himalayas, Pakistan. Integrating field observations, petrographic analysis, and laboratory experiments (uniaxial compressive strength (UCS), Brazilian [...] Read more.
This study examines the influence of tectonically induced mineralogical and microfabric changes on the strength of different rocks within the Hanzel Fault Damage Zone (FDZ) in the Tethyan Himalayas, Pakistan. Integrating field observations, petrographic analysis, and laboratory experiments (uniaxial compressive strength (UCS), Brazilian tensile strength (BTS), ultrasonic pulse-wave velocity (UPV), and porosity), this study systematically characterizes the spatial variations in intact rock strength across horizontal distance from the fault core to the outer limit of the FDZ. Seven rock units—granites (biotite granite, leucogranite schist, granodiorite schist, and diorite) and amphibolites (foliated amphibolite, amphibolite, and plagioclase amphibolite)—were sampled at varying distances (−500 to +4035 m) from the fault core. Results reveal that proximity to the fault core correlates with significant strength reductions (40%–70%): granitic rocks exhibit lower UCS (41–59 MPa) and BTS (4.8–6.7 MPa) compared to distal amphibolites and diorites UCS (75–107 MPa) and BTS (10–13.67 MPa). Petrographic analysis identifies key factors that reduce strength, including high mica content (up to 33%), pervasive micro-fracturing, S-C fabrics, and mineral alteration. These features increase porosity (up to 1.21%) and reduce UPV (2867–3315 m/s) in fault-proximal rocks. Moderate inverse relationships (R2 = 0.68–0.72) between mica percentage and UCS/UPV confirm phyllosilicates as primary strength controls. The spatial variation in rock strength is attributed to ductile–brittle deformation processes, with foliated or schistose textures increasing in proximity to the fault core. This study demonstrates that tectonic processes significantly influence the mineralogy and microfabric within FDZs, leading to variations in rock strength with direct implications for stability in tectonically active regions. Full article
(This article belongs to the Special Issue Characterization of Geological Material at Nano- and Micro-scales)
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22 pages, 21904 KB  
Article
Complex Network Modeling and Analysis of Microfracture Activity in Rock Mechanics
by Yushu Chen, Qihua Zhao, Jindong Xiang and Yi Peng
Appl. Sci. 2025, 15(10), 5242; https://doi.org/10.3390/app15105242 - 8 May 2025
Cited by 3 | Viewed by 936
Abstract
This study employs rock triaxial acoustic emission laboratory tests to investigate the activity of microfractures in plagiogranite from the Yebatan hydropower station dam area. By integrating interdisciplinary theories—including spatiotemporal single-link groups, fractal theory, complex networks, and graph theory—we develop a complex network model [...] Read more.
This study employs rock triaxial acoustic emission laboratory tests to investigate the activity of microfractures in plagiogranite from the Yebatan hydropower station dam area. By integrating interdisciplinary theories—including spatiotemporal single-link groups, fractal theory, complex networks, and graph theory—we develop a complex network model of rock microfractures. Results demonstrate that the microfracture network, characterized by the average degree (<k>) and clustering coefficient (<c>), undergoes distinct evolutionary stages during rock deformation and failure. The complex network parameters <k> and <c> undergo abrupt increases and decreases. These changes serve as characteristic indicators of the transition from stable to unstable states in rock deformation and failure, providing new insights into predicting rock failure and instability. Full article
(This article belongs to the Special Issue Advances and Technologies in Rock Mechanics and Rock Engineering)
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15 pages, 12464 KB  
Article
Acid-Extracted Hydrocarbon Anomalies and Significance in the Chaoshan Depression of the Northern South China Sea
by Guangjian Zhong, Jing Zhao, Zhongquan Zhao, Kangshou Zhang, Junhui Yu, Chunjiang Shang, Guanghong Tu and Changmao Feng
J. Mar. Sci. Eng. 2024, 12(6), 909; https://doi.org/10.3390/jmse12060909 - 29 May 2024
Cited by 1 | Viewed by 1351
Abstract
To predict the favorable zones and the types of reservoirs, acid extraction has been used in the Chaoshan depression to detect trace amounts of light hydrocarbons, heavy hydrocarbons, and the δ 13C (‰) of methane. As a result, two integration anomalous zones [...] Read more.
To predict the favorable zones and the types of reservoirs, acid extraction has been used in the Chaoshan depression to detect trace amounts of light hydrocarbons, heavy hydrocarbons, and the δ 13C (‰) of methane. As a result, two integration anomalous zones for exploration activity were blocked out in the northeastern and southwestern parts of the Chaoshan Depression, respectively. By analyzing the differentiation law and structural characteristics of hydrocarbon gases, as well as the stable carbon isotope ratio of methane, the underlying reservoirs were predicted to be gas reservoirs, and the seismically interpreted Dongsha-A (DS-A) structure was predicted to be a gas-rich structure. By correlating the seismic profile and geochemical anomalies, it was determined that fault planes and micro-fractures are the main controlling factors for the occurrence of the seabed’s geochemical anomalies. A composite formation mechanism of “lower generation, upper accumulation and micro fractures leaking” is proposed for the control of the underlying petroleum reservoirs, as well as for the micro-fracture control of permeability and surface adsorption control. Acid-extracted hydrocarbon anomalies have favorable indicating significance for exploration activity. Full article
(This article belongs to the Special Issue Advances in Marine Gas Hydrate Exploration and Discovery)
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14 pages, 2619 KB  
Article
Rehabilitation of Soccer Players’ Knee Injuries: Cartilage Reconstruction, Anterior Cruciate Ligament Surgery, and Intensive Recovery—A Pilot Study
by Bartłomiej Kacprzak and Karolina Rosińska
J. Clin. Med. 2023, 12(21), 6893; https://doi.org/10.3390/jcm12216893 - 1 Nov 2023
Cited by 6 | Viewed by 9698
Abstract
Knee injuries, particularly anterior cruciate ligament (ACL) damage and cartilage defects, are highly prevalent among athletes and affect their sports performance and long-term joint function. The purpose of this research was to evaluate the effectiveness of a comprehensive combination therapy approach for individuals [...] Read more.
Knee injuries, particularly anterior cruciate ligament (ACL) damage and cartilage defects, are highly prevalent among athletes and affect their sports performance and long-term joint function. The purpose of this research was to evaluate the effectiveness of a comprehensive combination therapy approach for individuals with ACL and cartilage injuries. Twelve professional soccer players aged 18 to 30 years underwent bone–tendon–bone ACL reconstruction, microfracture cartilage repair surgery, and hyaluronic acid scaffold treatment. Early postoperative rehabilitation included immediate supervised physiotherapy and complete weight bearing. Follow-up assessments involved clinical evaluations, functional joint assessments, and magnetic resonance imaging (MRI) scans to measure cartilage defect repair and symptom alleviation. The results showed that patients resumed pain-free activities within 3–4 weeks and returned to their pre-injury level within 4.5 months. MRI demonstrated the absence of inflammatory reactions, repair of marrow edema, and the emergence of new cartilage. Six months and one year after surgery, the Knee Injury and Osteoarthritis Outcome Score (KOOS) and the Short Form (36) Health Survey (SF-36) questionnaire results demonstrated considerable improvement in patients’ health condition and quality of life. Overall, the study suggests that the combination of Hyalofast membranes, microfracture surgery, tissue adhesive, and intensive postoperative physical therapy may be a potential alternative to commonly used treatments for patients with ACL rupture, allowing them to recover efficiently and return to sports activities. Full article
(This article belongs to the Special Issue Joint Surgery and Sports Medicine Research: In Health and Disease)
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15 pages, 15207 KB  
Article
Evaluation of the Effects of Nano-SiO2 Microemulsion on Decompression and Augmented Injection in the Eunan Tight Reservoir
by Ke Wu, Mingbiao Xu, Shoucheng Wen and Xuefeng Deng
AppliedChem 2023, 3(4), 477-491; https://doi.org/10.3390/appliedchem3040030 - 25 Oct 2023
Viewed by 1955
Abstract
The residual oil saturation of the matrix near the well zone of a tight reservoir is high due to the tight reservoir’s complex conditions, such as the small pore throat radius and low permeability of the matrix and the development of microfractures, which [...] Read more.
The residual oil saturation of the matrix near the well zone of a tight reservoir is high due to the tight reservoir’s complex conditions, such as the small pore throat radius and low permeability of the matrix and the development of microfractures, which can result in serious water channeling, even after long-term water injection development. The aim of this paper is to improve the effects of depressurization and augmented injection for tight reservoir waterflooding development by reducing the tight matrix’s residual oil saturation, increasing and maintaining its water phase permeability near the well zone using a nano-SiO2 microemulsion system with a small particle size and high interfacial activity. Therefore, four nano-microemulsion systems were evaluated and screened for their temperature resistance, salt resistance, interfacial tension, solubilization, and dilution resistance. A microemulsion system of 13% A + 4% B + 4% C + 4% n-butanol + 6% oil phase + 69% NaCl solution (10%) + 1% OP-5 + 0.5% anti-temperature agent + 0.3% nanosilica material was preferred. According to the core displacement experiment, the depressurization rate can reach 28~60% when the injection concentration of the system is 1~10% and the injection volume is 2~5 PV. The results of the on-site test show that the water injection pressure dropped to 17.5 MPa, which was lower than the reservoir fracture re-opening pressure. The pressure reduction rate was approximately 20%. The validity period of the depressurization and augmented injection has reached 23 months to date. Full article
(This article belongs to the Special Issue Feature Papers in AppliedChem)
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22 pages, 602 KB  
Review
Knee Cartilage Lesion Management—Current Trends in Clinical Practice
by Jaromir Jarecki, Marcin Krzysztof Waśko, Wojciech Widuchowski, Agnieszka Tomczyk-Warunek, Magdalena Wójciak, Ireneusz Sowa and Tomasz Blicharski
J. Clin. Med. 2023, 12(20), 6434; https://doi.org/10.3390/jcm12206434 - 10 Oct 2023
Cited by 27 | Viewed by 7108
Abstract
Many patients, particularly those aged above 40, experience knee joint pain, which hampers both sports activities and daily living. Treating isolated chondral and osteochondral defects in the knee poses a significant clinical challenge, particularly in younger patients who are not typically recommended partial [...] Read more.
Many patients, particularly those aged above 40, experience knee joint pain, which hampers both sports activities and daily living. Treating isolated chondral and osteochondral defects in the knee poses a significant clinical challenge, particularly in younger patients who are not typically recommended partial or total knee arthroplasty as alternatives. Several surgical approaches have been developed to address focal cartilage defects. The treatment strategies are characterized as palliation (e.g., chondroplasty and debridement), repair (e.g., drilling and microfracture), or restoration (e.g., autologous chondrocyte implantation, osteochondral autograft, and osteochondral allograft). This review offers an overview of the commonly employed clinical methods for treating articular cartilage defects, with a specific focus on the clinical trials conducted in the last decade. Our study reveals that, currently, no single technology fully meets the essential requirements for effective cartilage healing while remaining easily applicable during surgical procedures. Nevertheless, numerous methods are available, and the choice of treatment should consider factors such as the location and size of the cartilage lesion, patient preferences, and whether it is chondral or osteochondral in nature. Promising directions for the future include tissue engineering, stem cell therapies, and the development of pre-formed scaffolds from hyaline cartilage, offering hope for improved outcomes. Full article
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15 pages, 3588 KB  
Technical Note
Fracture Electromagnetic Radiation Induced by a Seismic Active Zone (in the Vicinity of Eilat City, Southern Israel)
by Vladimir Frid, Avinoam Rabinovitch, Dov Bahat and Uri Kushnir
Remote Sens. 2023, 15(14), 3639; https://doi.org/10.3390/rs15143639 - 21 Jul 2023
Cited by 4 | Viewed by 2623
Abstract
This paper deals with the quantitative analysis of measured fracture-induced electromagnetic radiation (FEMR) near the Dead Sea Transform using the Angel-M1 instrument, which enables the recording of FEMR signals in a 3D manner. The results showed both the possibility of estimating the sizes [...] Read more.
This paper deals with the quantitative analysis of measured fracture-induced electromagnetic radiation (FEMR) near the Dead Sea Transform using the Angel-M1 instrument, which enables the recording of FEMR signals in a 3D manner. The results showed both the possibility of estimating the sizes of micro-fractures that are the sources of radiation and assessing the direction of the fractures’ locations to the measuring device, as well as the range of magnitude (Mw) of the impending “events” (EQs) associated with the FEMR measurements. Moreover, the relation between the measured FEMR activity (the number of FEMR hits per unit of time) and the FEMR event magnitudes showed consistency with the Gutenberg–Richter relationship for the region. Such measurements could therefore constitute a preliminary ‘field reinforcement’ towards a valid EMR method for a real earthquake forecast, which would provide much earlier warnings than seismic methods. The observed FEMR measurements could only be used to assess the stress concentrations and micro-fracturing in the region since they related to the very initial nucleation phase of a “virtual” earthquake. Nonetheless, they provide the necessary feasibility test for a forecasting method since all of the lab-measured FEMR features were confirmed in the field. Full article
(This article belongs to the Special Issue Geological Applications of Remote Sensing and Photogrammetry)
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15 pages, 4096 KB  
Article
Hyalofast Cartilage Repair Surgery with a Full Load-Bearing Rehabilitation Program One Day after Operation Reduces the Time for Professional Athletes to Return to Play
by Bartłomiej Kacprzak, Karolina Rosińska and Natalia Siuba-Jarosz
Medicina 2023, 59(4), 804; https://doi.org/10.3390/medicina59040804 - 20 Apr 2023
Cited by 13 | Viewed by 6708
Abstract
Background and Objectives: This study evaluated the effectiveness of Hyalofast cartilage repair surgery with an early, full load-bearing rehabilitation program one day after the operation for reducing the time needed for professional athletes to return to play. Materials and Methods: This [...] Read more.
Background and Objectives: This study evaluated the effectiveness of Hyalofast cartilage repair surgery with an early, full load-bearing rehabilitation program one day after the operation for reducing the time needed for professional athletes to return to play. Materials and Methods: This prospective study included 49 patients aged between 19 and 38 years who had undergone surgical reconstruction of cartilage using the microfracture technique combined with a Hyalofast scaffold. All patients were active professional athletes. Early rehabilitation was implemented from the first postoperative day, fully loading the operated limb. A clinical evaluation was based on the KOOS and SF-36 questionnaires used during subsequent follow-up visits. All patients underwent magnetic resonance imaging (MRI) to evaluate the effect of the surgery after one year. Results: The clinical results demonstrated a statistically significant improvement in the number of complaints about pain and in the quality of life of the patients, measured in all of the applied scales, with comparisons made between six months or one year post-surgery and pre-surgery. Importantly for athletes, the parameter related to sports and recreation improved from 14 ± 11.1 to 95 ± 7.7 6 months after surgery and to 99.8 ± 1.8 one year after surgery. The overall quality of life score improved from 30 ± 18 to 88 ± 8.8 one year after surgery. Conclusions: These results show that this approach significantly shortened the time needed for the athletes to return to sports at the same level as before the surgery (athletes returned to sports in approximately 2.5–3 months). The mean follow-up time was 19.75 months. This technique can be considered a viable option for the treatment of cartilage injuries in professional athletes, allowing them to return to play more quickly in a safe and healthy way. Full article
(This article belongs to the Special Issue Advances in Orthopedics and Sports Medicine)
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11 pages, 29737 KB  
Article
The Efficacy of Microfracture Combined with Extracorporeal Shock Wave Therapy for Treating Osteochondral Lesion of the Talus and the Quality of Regenerated Cartilage: A Retrospective Cohort Study and MRI Assessment
by Jian Li, Qiaozhi Ma, Jianlei Hou, Yufen Liu, Pengfei Lu, Pengwei Liu, Zhongwen Zhang and Gengyan Xing
J. Clin. Med. 2023, 12(8), 2966; https://doi.org/10.3390/jcm12082966 - 19 Apr 2023
Cited by 5 | Viewed by 3589
Abstract
Background: osteochondral lesion of the talus (OLT) is a common disease in the physically active population, and extracorporeal shock wave therapy (ESWT) is a noninvasive treatment. We hypothesized that microfracture (MF) combined with ESWT may have great potential to become a novel combination [...] Read more.
Background: osteochondral lesion of the talus (OLT) is a common disease in the physically active population, and extracorporeal shock wave therapy (ESWT) is a noninvasive treatment. We hypothesized that microfracture (MF) combined with ESWT may have great potential to become a novel combination treatment of OLT. Methods: the OLT patients who received MF + ESWT or MF + platelet-rich plasma (PRP) injection were retrospectively included, with a minimal follow up of 2y. The daily activating VAS, exercising VAS, and American Orthopedic Foot and Ankle Society Ankle-Hindfoot Score (AOFAS) were used to assess the efficacy and functional outcome, and ankle MRI T2 mapping was used to evaluate the quality of regenerated cartilage in the OLT patients. Results: only transient synovium-stimulated complications were found during the treatment sessions; the complication rate and daily activating VAS did not have differences between groups. MF + ESWT had a higher AOFAS and a lower T2 mapping value than MF + PRP at the 2y follow up. Conclusions: the MF + ESWT had superior efficacy for treating OLT, which resulted in better ankle function and more hyaline-like regenerated cartilage, superior to the traditional MF + PRP. Full article
(This article belongs to the Section Orthopedics)
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17 pages, 2558 KB  
Article
Cartilaginous Metabolomics Reveals the Biochemical-Niche Fate Control of Bone Marrow-Derived Stem Cells
by Haining Peng, Yi Zhang, Zhongkai Ren, Ziran Wei, Renjie Chen, Yingze Zhang, Xiaohong Huang and Tengbo Yu
Cells 2022, 11(19), 2951; https://doi.org/10.3390/cells11192951 - 21 Sep 2022
Cited by 4 | Viewed by 3344
Abstract
Joint disorders have become a global health issue with the growth of the aging population. Screening small active molecules targeting chondrogenic differentiation of bone marrow-derived stem cells (BMSCs) is of urgency. In this study, microfracture was employed to create a regenerative niche in [...] Read more.
Joint disorders have become a global health issue with the growth of the aging population. Screening small active molecules targeting chondrogenic differentiation of bone marrow-derived stem cells (BMSCs) is of urgency. In this study, microfracture was employed to create a regenerative niche in rabbits (n = 9). Cartilage samples were collected four weeks post-surgery. Microfracture-caused morphological (n = 3) and metabolic (n = 6) changes were detected. Non-targeted metabolomic analysis revealed that there were 96 differentially expressed metabolites (DEMs) enriched in 70 pathways involved in anti-inflammation, lipid metabolism, signaling transduction, etc. Among the metabolites, docosapentaenoic acid 22n-3 (DPA) and ursodeoxycholic acid (UDCA) functionally facilitated cartilage defect healing, i.e., increasing the vitality and adaptation of the BMSCs, chondrogenic differentiation, and chondrocyte functionality. Our findings firstly reveal the differences in metabolomic activities between the normal and regenerated cartilages and provide a list of endogenous biomolecules potentially involved in the biochemical-niche fate control for chondrogenic differentiation of BMSCs. Ultimately, the biomolecules may serve as anti-aging supplements for chondrocyte renewal or as drug candidates for cartilage regenerative medicine. Full article
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9 pages, 247 KB  
Article
Sport Activity and Clinical Outcomes after Hip Arthroscopy with Acetabular Microfractures at a Minimum 2-Year Follow-Up: A Matched-Pair Controlled Study
by Mattia Loppini, Francesco La Camera, Francesco Manlio Gambaro, Riccardo Ruggeri, Guido Grappiolo and Federico Della Rocca
Life 2022, 12(8), 1107; https://doi.org/10.3390/life12081107 - 22 Jul 2022
Cited by 4 | Viewed by 1952
Abstract
Background: Acetabular microfractures for cartilage lesions have been shown to be a safe procedure able to improve patient reported outcomes (PROs). Nevertheless, the return to sport activity rate represents a crucial outcome to be investigated in these young athletic patients. Methods: [...] Read more.
Background: Acetabular microfractures for cartilage lesions have been shown to be a safe procedure able to improve patient reported outcomes (PROs). Nevertheless, the return to sport activity rate represents a crucial outcome to be investigated in these young athletic patients. Methods: Patients undergoing acetabular microfracture for full chondral acetabular lesions were compared to a 1:1 matched-pair by age and gender control group undergoing hip arthroscopy without microfractures. Clinical assessment was performed with PROs and participation in sports in terms of type and level of activities was evaluated preoperatively and at 2-years follow-up. Results: A total of 62 patients with an average age of 35.1 ± 8.1 (microfracture group) and 36.4 ± 6.3 (control group) were included. In both groups, the average values of PROs significantly increased from preoperatively to the last follow-up. There was no significant difference between the two groups in the number of patients playing at the amateur and elite level preoperatively and at the last follow-up. Conclusions: Microfractures for the management of full-thickness acetabular chondral defect provides good clinical results at a minimum follow-up of two years, which are not inferior to a matched-pair control group. Patients undergoing this procedure are likely able to return at the same level of sport before surgery. Full article
(This article belongs to the Special Issue Orthopaedic Trauma and Reconstruction)
13 pages, 3093 KB  
Article
Laboratory Rat Thrombi Lose One-Third of Their Stiffness When Exposed to Large Oscillating Shear Stress Amplitudes: Contrasting Behavior to Human Clots
by Ursula Windberger, Veronika Glanz and Leon Ploszczanski
Int. J. Transl. Med. 2022, 2(3), 332-344; https://doi.org/10.3390/ijtm2030026 - 12 Jul 2022
Cited by 2 | Viewed by 4660
Abstract
Rats impress by their high platelet count resulting in hypercoagulability, which protects the animals from severe bleeding. However, platelets also import numerous stiff junction points into the fibrous system of a clot, also enhancing the pre-stress of the fibrin fibers, which lowers their [...] Read more.
Rats impress by their high platelet count resulting in hypercoagulability, which protects the animals from severe bleeding. However, platelets also import numerous stiff junction points into the fibrous system of a clot, also enhancing the pre-stress of the fibrin fibers, which lowers their deformability. Clot deformation is clinically important since large strains are present in the arterial tree (caused by the propagation of pressure and pulse waves), and a clot is considered “safe” when it can deform over a long range of strain amplitudes. We tested clot formation and the behavior of fully formed blood clots of laboratory rats at large sinusoidal shear stress amplitudes by rheometry and compared outcomes to human reference data. We found that fiber density (by scanning electron microscopy) and clot stiffness (by rheometry) was pronounced compared to humans and differed with sexual dimorphism and with rat strain. Using our large amplitude oscillation (LAOS) protocol, we detected that rat clots yielded with a frustrated attempt to stiffen instead of showing the macroscopic stiffening response that is typical for human clots. We attribute this behavior to the appearance of multiple microfractures until, finally, a few leading fibers uptake the load. Rat clots also failed to align fibers in shear direction to initiate affine deformation. The rat clot phenotype differs substantially from the human one, which must be considered in research and toxicological testing. If microfractures in the fiber meshwork are concentrated in vivo, parts of a clot may break off and be washed away. However, homogenously distributed microfractures may open pores and allow the penetration of plasminogen activators. What occurs in the rat vasculature depends on the on-site clot composition. Full article
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