Search Results (35)

The growth and development of aquaculture organisms are significantly influenced by environmental variations shaped by different aquaculture systems. In this study, a 90-day controlled experiment was conducted to compare two pond culture setups for largemouth bass: with water hyacinth co-planted (FM group) and without (M group). As this experiment progressed, the FM group exhibited significantly superior water quality (p < 0.05) compared to the M group across multiple parameters, including total nitrogen (TN), total phosphorus (TP), ammonia nitrogen (NH₃-N), dissolved oxygen (DO) and transparency, among which, the difference in transparency was especially evident (p < 0.001). Subsequently, by 90 days, the fish body weight, condition factor, and gonadosomatic index (GSI) were significantly higher in the FM group than in the M group, with the GSI difference being particularly pronounced (p < 0.001). While the GSI of M group fishes ranged exclusively from 0.01 to 0.02 (M1), the FM group displayed a much-expanded GSI range of 0.01–0.06, with 21.4% at 0.01–0.02 (FM1), 48.1% at 0.02–0.03 (FM2), and 30.5% at 0.03–0.06 (FM3). Accordingly, omics analyses of ovarian tissues were conducted between the control (M1) and the high-performing groups (FM2 and FM3). The analyses identified significant enrichment of the glycerophospholipid metabolic pathway and a marked upregulation of the Mettl3 gene (log2FC = 12.59) in the FM2 and FM3 than the M1 group, and both the pathway and the Mettl3 gene were actively involved in growth, reproductive processes, and oocyte maturation. Given that water transparency was the most markedly improved parameter, our results indicate that it may be a key driver in upregulating ovarian glycerophospholipid metabolism and Mettl3 expression in largemouth bass, thereby promoting better growth and ovarian development. Full article

Facultative paedomorphosis, the retention of larval traits in sexually mature individuals, plays a crucial role in species ecology and evolution and is influenced by complex interactions between environmental factors. Here, we compile all known cases of paedomorphosis in all newt species in Greece and report 20 new localities, mainly in Northern Pindos National Park. Our results indicate that paedomorphosis tends to occur more frequently in stable aquatic environments in combination with unfavourable external conditions (lack of precipitation and higher temperatures). Furthermore, species-specific patterns related to the occurrence of paedomorphosis were also unveiled: Mesotriton alpestris prefers high-elevation and permanent ponds; Lissotriton graecus occurs predominantly in artificial, lowland ponds; and Triturus macedonicus is associated with a stable hydroperiod and fish absence. Overall, conservation strategies should explicitly account for paedomorphic populations, emphasizing the value of artificial ponds, which are able to support this life-history strategy. Lastly, the Northern Pindos Mountain Range emerges as a major European intra-specific diversity hotspot. Full article

Rainfall deficits and erratic dry spells pose major challenges in rainfed ecosystem. In-situ moisture conservation practices (MCP) like ridge–furrow methods, improve soil moisture but are inadequate during 2–3 week dry spells at critical crop stages (flowering and maturity), leading to yield loss. Supplemental irrigation (SI) using an ex-situ rainwater harvesting (RWH) pond can mitigate these effects, but optimizing the pond design is challenging due to limited runoff and storage losses. This study aims to design RWH pond for small farm holders with a 1.0 ha area and evaluate its efficient use for SI during intermittent dry spells and critical crop stages. The design volume was estimated using the SCS-CN method based on daily rainfall data (1974–2010) for the northeast monsoon. A pond with a capacity of 487.5 m³, constructed for a 1 ha micro-watershed, was used to observe the runoff for design validation. The harvested runoff can be used as SI for a cultivable area of 0.4 ha, based on the watershed-to-cultivable area ratio. Statistical analysis of observed and estimated runoff data from 2011 to 2023 revealed a strong correlation (r = 0.87), confirming the pond design. Harvested rainwater, applied through micro-irrigation (rain gun) at a depth of 50 mm during moisture stress periods, significantly improved cotton productivity. The combined use of harvested rainwater and MCP increased yield in the range of 3.8 to 25.3%, improved rainwater use efficiency (1.52 to 3.13 kg ha⁻¹ mm⁻¹), and had a higher benefit-cost ratio (1.15 to 2.43) over a 13-year period. This study concludes that integrating in-situ MCP with ex-situ RWH with micro-irrigation significantly improves rainfed crop productivity in vertisols. Full article

This study investigated the influence of two distinct aquaculture systems, namely, the wheat-crab model (WCM) and pond culture (PC) model, on the growth, physiological well-being, and gut microbial structure of Chinese mitten crabs (Eriocheir sinensis). A total of 120 adult crabs were randomly selected from the two systems: 60 crabs from the WCM, including 30 females and 30 males, and 60 crabs from the PC model, also including 30 females and 30 males. The gonadosomatic index of female crabs in the WCM was notably higher than that of the other groups, while the hepatopancreatic index was significantly lower. Significant variations were not observed in final weight, fullness, or muscle yield between the WCM and PC groups. Biochemical evaluations indicated no substantial differences in antioxidant capabilities between the two systems; however, female crabs demonstrated increased critical antioxidant enzyme activity, such as for catalase and superoxide dismutase, and significantly elevated cholesterol levels. Additionally, the expression of the genes IL, ProPO, and Keap1 was significantly higher in the WCM group than the PC group, whereas the expression of ALF2, Myd88, and CncC did not significantly differ between the two cultivation methods. Non-targeted metabolomics analysis revealed notable distinctions in metabolite profiles between the two systems. Moreover, the WCM facilitated an increase in beneficial Firmicutes bacteria while reducing potentially harmful microorganisms, suggesting improved immune function in crabs raised under the WCM. In summary, female crabs reared in the WCM matured earlier and exhibited slightly better health conditions compared to those grown in the PC model. Full article

CIFA-GI Scampi^® is a genetically improved, fast-growing strain of giant river prawn (Macrobrachium rosenbergii) developed by ICAR-Central Institute of Freshwater Aquaculture. Each year, selected broodstock are reared family-wise in nylon hapas for 3–4 months before use as parents for the next generation. However, this group rearing phase (GR) results in loss of quality broodstock due to aggression and cannibalism. This study evaluated individual rearing (IR) as an alternative to group rearing (GR). GR involved rearing of males or females in separate hapas, while IR placed males and females in separate hard plastic mesh boxes in ponds (18 boxes each). Results showed significantly higher average daily weight gain in GR, but IR achieved 100% survival for both sexes, compared to 50% (males) and 77.8% (females) in GR. No significant differences were observed in female maturity or male morphotypes (small male and no claw morphotypes). Given the markedly higher survival in IR, it is a viable alternative for post-selection rearing to minimize loss of valuable broodstock in the selective breeding program. Full article

An analysis of the age structure of three water frog species (P. lessonae, P. ridibundus, P. esculentus) in the Middle Volga region of Russia was conducted. The skeletochronological method determined their maximum lifespans to be 7, 6, and 4 years, respectively. This study revealed specific characteristics of the age structure of Pelophylax frogs in relation to their taxonomic composition when cohabiting (population system type) and the degree of habitat transformation. Differences in body size and lifespan were observed among cryptic forms exhibiting mitochondrial DNA replacement and the presence of various types of heterozygous and homozygous alleles. Water frogs of the genus Pelophylax have a complex taxonomic structure, including hybrid species and morphologically cryptic forms, as well as massive mitochondrial DNA introgression. The aim of our paper was to analyze the age structure of three species of water frogs (P. lessonae, P. ridibundus, and P. esculentus) in the Middle Volga River region (European Russia). Based on the skeletochronological method, we determined their maximum lifespans of seven, six, and four years, respectively. The onset of sexual maturity in the marsh frogs occurred after two and four winterings in males and females (respectively), in the pond frog—one to two years, and in the edible frog—one to three years. This study revealed specific characteristics of the age structure of Pelophylax frogs in relation to their taxonomic composition when cohabiting (population system type) and the degree of habitat transformation. Differences in body size and lifespan were observed among cryptic forms exhibiting mitochondrial DNA replacement and the presence of various types of heterozygous and homozygous alleles. Full article

Acrostichum aureum is a halophytic pantropical invasive fern growing in mangroves and swamps. Its association with arbuscular mycorrhizal fungi (AMF) has been reported in Asia. AMF and their symbiosis (AM) commonly colonise the absorption organs of terrestrial plants worldwide. Furthermore, AMF/AM are well known for their capacity to bioaccumulate toxic elements and to alleviate biotic and abiotic stress (e.g., salinity stress) in their hosts. However, the mechanisms underlying AMF involvement in the halophytism of A. aureum and the structures where NaCl accumulates remain unknown. This study shows that A. aureum forms AM in margins of natural thermal ponds in Neotropical wetlands. All mature sporophytes were colonised by AMF, with high percentages for root length (ca. 57%), arbuscules (23), and hyphae (25) and low values for vesicles (2%). In A. aureum–AMF symbiosis, NaCl accumulated in AMF vesicles, and CaSO₄ precipitated in colonised roots. Therefore, AM can contribute to the halophytic nature of this fern, allowing it to thrive in saline and thermal environments by capturing NaCl from fern tissues, compartmentalising it inside its vesicles, and precipitating CaSO₄. Full article

Rice is a water-intensive crop, conventionally grown under submerged conditions, with standing water for about 80% of its growth period. There is an urgent need for water-saving technologies to address challenges associated with conventional irrigation techniques for rice. The alternate wetting and drying (AWD) technique is one of these water-saving techniques; however, it requires continuous monitoring of water levels in the field. The implementation of real-time, electronic sensor-based precision irrigation technology may address the problems associated with conventional irrigation systems and AWD leading to high water use efficiency. Therefore, a study was undertaken to develop a suitable sensor-based automated irrigation system to maintain optimal water levels in rice fields. This study conceptualized an electronic sensor-based automated irrigation system for rice cultivated under the AWD technique. In this method, the rice field is initially flooded to a maximum depth of 5 cm. Irrigation is reapplied once the water level reduces to 10 cm below the soil surface. This developed system helps address water scarcity by regulating water levels, preventing excess ponding. It uses magnetic float-based sensors and electronic circuits to detect water levels, converting them into electronic signals transmitted wirelessly via radio frequency (RF) to a controller. The controller has been programmed for different growth stages that need to be set manually during the cropping period. The system is designed primarily for the AWD method but includes an option for continuous ponding (CP), needed during the flowering stage. The maximum water level at full maturity is set at 5 cm above the soil surface, while irrigation with the AWD method begins when the water level falls 10 cm below the soil surface. The developed system was tested during the Kharif season of 2018–19; the irrigation water productivity was 6.15 kg ha⁻¹mm⁻¹ with the automated system, compared to 3.06 kg ha⁻¹mm⁻¹ in the control (continuous ponding). Total water productivity was 4.80 kg ha⁻¹mm⁻¹ for the automated system and 2.63 kg ha⁻¹mm⁻¹ for the control. The automated system achieved 36% more water savings over the control, which used continuous ponding as farmers practice. The developed system supports AWD, a proven water-saving technique in rice cultivation. Full article

Lotus seedpod maturity detection and segmentation in pond environments play a significant role in yield prediction and picking pose estimation for lotus seedpods. However, it is a great challenge to accurately detect and segment lotus seedpods due to insignificant phenotypic differences between the adjacent maturity, changing illumination, overlap, and occlusion of lotus seedpods. The existing research pays attention to lotus seedpod detection while ignoring maturity detection and segmentation problems. Therefore, a semantic segmentation dataset of lotus seedpods was created, where a copy-and-paste data augmentation tool was employed to eliminate the class-imbalanced problem and improve model generalization ability. Afterwards, an improved YOLOv8-seg model was proposed to detect and segment the maturity of lotus seedpods. In the model, the convolutional block attention module (CBAM) was embedded in the neck network to extract distinguished features of different maturity stages with negligible computation cost. Wise-Intersection over Union (WIoU) regression loss function was adopted to refine the regression inference bias and improve the bounding box prediction accuracy. The experimental results showed that the proposed YOLOv8-seg model provides an effective method for “ripe” and “overripe” lotus seedpod detection and instance segmentation, where the mean average precision of segmentation mask (${mAP}_{mask}$) reaches 97.4% and 98.6%, respectively. In addition, the improved YOLOv8-seg exhibits high robustness and adaptability to complex illumination in a challenging environment. Comparative experiments were conducted using the proposed YOLOv8-seg and other state-of-the-art instance segmentation methods. The results showed that the improved model is superior to the Mask R-CNN and YOLACT models, with recall, precision, ${mAP}_{box}$ and ${mAP}_{mask}$ being 96.5%, 94.3%, 97.8%, and 98%, respectively. The average running time and weight size of the proposed model are 25.9 ms and 7.4 M, respectively. The proposed model obtained the highest mAP for lotus seedpod maturity detection and segmentation while maintaining an appropriate model size and speed. Furthermore, based on the obtained segmentation model, 3D visualization of the lotus pond scene is performed, and cloud point of lotus seedpods is generated, which provides a theoretical foundation for robot harvesting in the lotus pond. Full article

Habitat restoration and creation within human-altered landscapes can buffer the impacts of climate change on wildlife. The Forestry Reclamation Approach (FRA) is a coal surface mine reclamation practice that enhances reforestation through soil decompaction and the planting of native trees. Recently, wetland creation has been coupled with FRA to increase habitat available for wildlife, including amphibians. Our objective was to evaluate the response of pond-breeding amphibians to the FRA by comparing species occupancy, richness, and abundance across two FRA age-classes (2–5-year and 8–11-year reclaimed forests), traditionally reclaimed sites that were left to naturally regenerate after mining, and in mature, unmined forests in the Monongahela National Forest (West Virginia, USA). We found that species richness and occupancy estimates did not differ across treatment types. Spotted Salamanders (Ambystoma maculatum) and Eastern Newts (Notophthalmus viridescens) had the greatest estimated abundances in wetlands in the older FRA treatment. Additionally, larger wetlands had greater abundances of Eastern Newts, Wood Frogs (Lithobates sylvaticus), and Green Frogs (L. clamitans) compared to smaller wetlands. Our results suggest that wetland creation and reforestation increases the number of breeding sites and promotes microhabitat and microclimate conditions that likely maximize the resilience of pond-breeding amphibians to anticipated climate changes in the study area. Full article

Aquaculture supplies high-quality and healthy proteins. With the increasing human demand for aquaculture production, intensive pond aquaculture developed rapidly and results in environmental deterioration. To solve this problem, the eco-substrate (ES), which is the biofilm carrier, has been utilized in aquaculture ponds. Studying the ecological mechanisms of ES from the perspective of the ecosystem may be conducive to the sustainable development of aquaculture. In this study, it was evaluated how ES makes a difference to the trophic structure, energy flow, and system characteristics of two different aquaculture pond ecosystems via the ecopath model. Three aquaculture ponds with ES were designed as the treatment ecosystem and three aquaculture ponds without ES were designed as the control ecosystem. There were 13 and 14 functional groups in the control and treatment ecosystems, respectively. The results showed that (1) the macrozooplankton and microzooplankton showed strong effects on the ecosystem in the keystoneness index; (2) energy transfer pathways in the treatment system with ES increased by 26.23% compared to the control system; (3) the ES improved the utilization rate of detritus, which was 14.91% higher than that of the control ecosystem; (4) the material and energy flow index and network information characteristics demonstrated the ES enhanced the complexity and stability of the treatment system. To improve the energy utilization efficiency, filter feeders can be introduced to ES ponds. Overall, the ES can alter the trophic structure, improve the energy utilization efficiency, and enhance the stability and maturity of aquaculture ecosystems, representing a sustainable practice. Considering the total area of aquaculture ponds on the earth reaching more than 5 million hectares, the application prospect of ES is broad. Full article

Insulin-like growth factors (IGFs) play central roles in the growth and development of vertebrates. Blotched snakehead (Channa maculata), an economically significant fish, exhibits obvious sexual dimorphism and achieves sexual maturity in one year. However, the role of IGFs in C. maculata remains unknown. Three IGF genes were identified in C. maculata, designated as CmIGF1-1, CmIGF1-2, and CmIGF2. The cDNA sequences of these genes are 1184, 655, and 695 bp, encoding putative proteins of 168, 131, and 215 amino acids, respectively, and all three proteins contain a conserved IGF domain. Quantitative real-time PCR (qPCR) revealed the predominant expression of CmIGFs in the liver of adult fish, with higher expression levels observed in males. Notably, CmIGF1-1, CmIGF1-2, and CmIGF2 displayed analogous expression profiles in the liver across various developmental stages, peaking at 365 days after hatching (dah). Subsequently, 600 individuals at 75 dah, at an early developmental stage, were randomly divided equally into six groups and reared in aerated 2 m × 2 m × 2 m cement ponds at 26.0 ± 1.0 °C. Following a one-week acclimatization period, fish without observed abnormalities were intraperitoneally injected with either 17α-ethynylestradiol (EE₂) or 17α-methyltestosterone (MT) at a dose of 10 μg/g body weight. Three groups underwent short-term hormone treatment, and the remaining three groups underwent long-term hormone treatment, which included five injections at two-week intervals over ten weeks. The analysis of CmIGFs expression levels in the liver under different hormone treatments revealed that EE₂ suppressed the expression of CmIGF1-1 and CmIGF1-2 while promoting CmIGF2 expression. In females, MT up-regulated the expression of CmIGF1-1 and CmIGF2 in a time-dependent manner, but consistently inhibited CmIGF2 expression. In males, MT promoted the expression of CmIGFs in a time-dependent manner, reaching peak levels for CmIGF1-1, CmIGF1-2, and CmIGF2 after 8, 10, and 2 weeks of injection, respectively. Additionally, CmIGF1 and CmIGF2 might exhibit a complementary relationship, with a compensatory increase in CmIGF2 expression in response to low CmIGF1 concentration. These findings highlight the potential key role of IGFs upon growth and their regulation by sex steroid hormones in C. maculata, providing a crucial foundation for future research aimed at elucidating the molecular mechanisms underlying the growth dimorphism between female and male blotched snakeheads. Full article

Mature students in postsecondary education—those over 25 years of age, also known as nontraditional students or adult learners—make up a significant minority population on Canadian postsecondary campuses. Despite academic performance that tends to be higher than that of traditional students, the retention of mature students is lower due to the stressors they face in their home and school roles. This paper examines the insights of mature students and their intimate partners on how higher education institutions can provide better supports to make postsecondary programs and campuses more accessible to adult learners and enable their success. A sample of 25 mature postsecondary students enrolled in Canadian higher education institutions and their partners participated in dyadic interviews that explored the connections between their relationship and school experiences. A data-driven, inductive thematic analysis of the transcribed interviews found three themes that focused on the institutional support needs of partnered mature students, highlighting the need to (1) increase institutional knowledge and awareness of mature students’ needs, (2) offer flexible study options to complete courses and program requirements, and (3) provide relevant supports and programs. Mature students felt marginalized at their institutions related to the programs, instructors, staff, resources, and supports that are strongly focused on traditional-aged and circumstanced students. Recommendations are provided for higher education institutions to provide resources and supports that meet mature students’ unique needs to both access and be successful in their pursuit of postsecondary education. Full article

Landfill leachate is regarded as a significant point source of pollutants that may pose a hazard to the environment, particularly to surface and ground waters. Leachates are highly variable and heterogeneous. Our study was focused on the characterization of landfill leachates derived from young (YDS) and matured (MDS) dumpsites in terms of the abundance of micropollutants, antimicrobial resistance genes (ARG), and microbial community structure. The concentrations of poly- and perfluoroalkyl substances (PFASs) were found to be higher in MDS compared to YDS, i.e., 13.19 and 7.16 μg/L, respectively. Among pharmaceutical compounds, ibuprofen was detected at the highest concentrations, i.e., 12.54, 12.81, and 13.13 μg/L, in the leachates derived from MDS, YDS, and ponds. The distribution of bacteria, archaea, eukaryotes, and viruses in the three leachate samples was as follows: 85.15 ÷ 88.04%, 9.13 ÷ 12.29%, 2.20 ÷ 2.68%, and 0.12 ÷ 0.15%, respectively. In total, 31 distinct families of ARGs were identified, comprising a total of 80 ARGs. Incubation of P. putida MSCL650 in sterile leachate from the pond resulted in decreasing the minimum inhibitory concentrations for six antibiotics as compared to cells incubated in nutrient broth. Hydrological processes, i.e., runoff and infiltration, can increase the dissemination of ARGs. Full article

The intensive use of antibiotics (for human, veterinary, and agricultural purposes) has steadily increased over the last 30 years. Large amounts of antibiotic residues are released into aquatic systems, mostly due to inefficient wastewater treatment. Conventional wastewater treatments are not designed to remove emerging contaminants (such as antibiotics) from wastewater. Therefore, algae treatment (phycoremediation) has emerged as a promising choice for cost-effective, eco-friendly, and sustainable wastewater treatment. For this reason, we investigated the removal performance of a well-established algal consortia (Chlorella protothecoides and Chlorella vulgaris) used in passive wastewater treatment ponds (Mosselbay, South Africa). Five antibiotics (sulfamethoxazole, amoxicillin, trimethoprim, ofloxacin, and clarithromycin) were selected for their ubiquity and/or low removal efficiency in conventional wastewater treatment plants (WWTPs). For each antibiotic, two concentrations were used: one environmentally relevant (10 ppb) and another 10 times higher (100 ppb), tested in triplicate and collected at two-time points (7 and 10 days). The algae remained viable over the exposure period (which is similar to the retention time within maturation ponds) and exhibited the capacity to remove sulfamethoxazole (77.3% ± 3.0 and 46.5% ± 5.3) and ofloxacin (43.5% ± 18.9 and 55.1% ± 12.0) from samples spiked with 10 and 100 ppb, respectively. This study demonstrates the potential and innovation of algal remediation for contaminants in a developing country context, where minimal infrastructure is available. Full article