Search Results (205)

The consumption of low-alcohol fermented beverages has been related to cardiovascular health improvements. Although the underlying mechanism is not completely understood, (poly)phenols have been proposed as one of the mediators. The objective of this study was to evaluate the impact of a controlled intervention with beer on (poly)phenols metabolism in individuals with and without metabolic syndrome (MetS). 20 participants (MetS and control) who consumed a standardized amount of beer during 6 weeks were recruited. Phenolic compounds were assessed in urine. Different changes in phenolic compounds associated with chronic beer consumption were found, particularly related to hesperetin conjugates and to the degradation of phenolic compounds derived from flavonoids and lignans. Noteworthily, MetS and control participants differed in baseline urine phenolic compound profiles and in their metabolization. Significant differences were found in the production and excretion of key (poly)phenols-derived metabolites, such as increased naringenin phase II conjugates in healthy subjects, or increased bacterial flavonoid catabolites. Certain relationships were observed between the phenolic compounds with metabolic and anthropometric variables. These findings suggest that beer-derived (poly)phenols are differentially metabolized according to metabolic-health status, and that they may contribute to certain metabolic health benefits through the modulation of specific metabolic pathways. Full article

Olive mill wastewater is a by-product of olive oil extraction, characterized by a high concentration of organic matter, which presents a significant environmental challenge if not properly managed. This study was aimed at valorizing olive mill wastewater through selective fermentations to produce acetic beverages with low or no alcohol content. Olive mill wastewaters at three different dilutions (100%, 75% and 50%) were inoculated with Saccharomyces cerevisiae UMCC 855 for alcoholic fermentation. The resulting alcoholic product, with 75% olive mill wastewater, was then used as a substrate for acetic acid fermentation by Acetobacter pasteurianus UMCC 1754, employing both static and submerged acetification systems. The results showed that, at the end of the static acetification process, no residual ethanol was detected and that high concentrations of acetic and gluconic acid (46.85 and 44.87 g/L, respectively) were observed. In the submerged fermentation system, the final ethanol concentration was 24.74 g/L; the produced organic acids content reached 31.63 g/L of acetic acid and 39.90 g/L of gluconic acid. Furthermore, chemical analyses revealed that fermentation enhanced the antioxidant activity of olive mill wastewater. These results suggest promising insights for the valorization of olive mill wastewater. Full article

Alcoholic beverages have been consumed for centuries in different countries around the world. Today, we know that the ethanol they contain is associated with significant health risks, especially in the case of abuse, in individuals with special health conditions, and in pregnant women. However, over the years, awareness has grown that wine, especially red wine, has a beneficial effect on human health due to the powerful effect of the antioxidant substances it contains, known under the generic term of polyphenols. The main concern remains around the ethanol content of wine and its effects on health. After fifty years of research and studies, the debate is still open, with conflicting indications about the positive effect of moderate wine consumption in the context of a balanced diet and the toxic effect of ethanol even in low doses. In this disputed area, the market for low-alcohol and alcohol-free wines has found its place in the last decade, creating a new opportunity for the global wine trade. These new types of wine are going to open a new chapter in the history of wine. In this review, we have summarised the main aspects of the health implications of wine consumption considering scientific evidence from the last 50 years, including low-alcohol and dealcoholised wine. Full article

Fermentation is widely recognized for enhancing the sensory attributes and nutritional value in foods, with recent research focusing on non-alcoholic and root-based functional beverages. In this study, the genomic and fermentation characteristics of Kluyveromyces marxianus LRCC8279 (KM8279) and Saccharomyces cerevisiae LRCC8293 (SC8293) were analyzed, specifically for their application in root extract-based low-alcohol fermentations. Whole-genome sequencing revealed that both strains harbored key genes involved in glucose, fructose, and sucrose metabolism and genes implicated in ethanol production. Although SC8293 harbored maltose-metabolizing genes, including MAL13 and MAL31, these genes were absent in KM8279. This genetic difference was evident in the fermentation performance, manifesting as distinct variations in alcohol production depending on the carbohydrate source. A further investigation of fermentation conditions demonstrated that both strains maintained low alcohol levels and exhibited a consistent growth at 15–20 °C within 72 h. Fermentation using extracts from Pueraria lobata, Arctium lappa (AL), Zingiber officinale (ZO), and Platycodon grandifloras revealed that KM8279 markedly increased the production of volatile compounds, contributing to floral and fruity sensory attributes in ZO and AL, whereas SC8293 contributed to a more complex flavor profile in AL. Notably, KM8279-ZO and KM8279-AL fermentations maintained alcohol contents below 1%, indicating their potential application in non-alcoholic beverages. Future studies are needed to investigate the relationship between the key volatile compound production and associated genetic characteristics, along with sensory evaluations, to develop optimized flavor modulation strategies. Full article

This study aimed to identify kiwi kefir-like beverages with high levels of viable probiotic cells and low levels of calories, acids, and alcohol. To achieve this, microbiological and chemical characterizations were conducted on beverages inoculated with varying amounts of kefir grains (GW) and incubated at different agitation speeds (A), following a second-order orthogonal factorial design. For each experimental condition, three 24-h batch cultures were performed using three successive passages of kefir grains. Higher GW levels promoted greater nutrient consumption and metabolite production. However, an intermediate GW (1.80 g) resulted in the highest growth of lactic acid bacteria (LAB), acetic acid bacteria (AAB), yeasts, and free biomass in the fermented medium. Optimal agitation levels also enhanced nutrient consumption, free biomass, and metabolite pro-duction. AAB and yeast counts increased with higher agitation speeds, while LAB counts de-creased. Three beverages, produced during the second (A = 86 rpm, GW = 2.81 g) and third (A = 38 rpm, GW = 2.60 g; A = 86 rpm, GW = 1.80 g) kefir grain passages, exhibited LAB and yeast counts above 10⁶; CFU/mL, along with low total sugar and ethanol concentrations. These beverages may be considered suitable as potentially probiotic, low-alcohol, and low-calorie functional drinks. Full article

By conducting a narrative review of the scientific literature, the authors of this study sought to verify whether there were sufficient data to answer the following question: “Can wine positively or negatively influence the incidence and severity of disorders associated with gastrointestinal (GI) diseases?”. In this review, most of the studies considered tested different alcoholic beverages (other than wine), not always reporting in the conclusions the possible difference in the extent of symptoms. Although alcohol certainly plays a central role in influencing the oesophageal and gastric environment, no studies evaluating the role of alcohol as such were included, since the aim of the review was to understand whether wine can be moderately consumed by patients with gastrointestinal diseases. The analysis of studies selected from the main reference databases indicates that even moderate wine consumption can be a source of discomfort in subjects with the GI diseases included in this review (gastritis and gastroesophageal disease, gastrointestinal motility, inflammatory bowel disease, irritable bowel syndrome, and microscopic colitis). This does not mean that a certain percentage of patients cannot tolerate moderate amounts of alcoholic beverages; however, discussion with the family doctor or specialist is essential to identify the correct diet in which to include or exclude the consumption of wine. One of the limitations of this review is the low number of studies available, at least for some of the pathologies considered. It is important to emphasise, however, that some selected epidemiological studies, which include many subjects (even over 100,000), can provide useful information from a scientific point of view. Full article

Many popular alcoholic beverages, such as Brazilian sugar cane spirit (cachaça), are aged in wood casks to achieve a smoother and more pleasant taste. The type of wood plays an important role in improving the quality of the spirit, with oak being the most widely used. Due to its elevated price and poor local availability, oak has been gradually replaced in Brazil by other woods, such as Amburana cearensis (Amburana), Cariniana legalis (Jequitibá), Hymenaea courbaril (Jatobá), and Ocotea odorifera (Cinnamon sassafras). For general purposes in beverage quality control and wood identification, and using ethanol/water extracts (cachaça 47% v/v) as a model, this article describes the construction of a low-cost electronic nose that quickly identifies the wood species that was used for aging a cachaça sample. The nose is made of an array of four chemoresistive conductive polymer gas sensors. Principal component and leave-one-out analyses showed perfect classification of all tested samples. Full article

Reverse osmosis (RO) and nanofiltration (NF) membranes are traditionally employed in wine and beer production for concentration, clarification, and stabilization. Their applications now extend to dealcoholization, addressing rising demand for low-alcohol beverages. RO/NF selectively reduce ethanol while retaining volatile aromas and non-volatile flavors, outperforming thermal methods that degrade sensory profiles. This review examines RO/NF roles in alcohol adjustment, sugar modification, and by-product recovery, emphasizing mechanisms and efficiency. Operational challenges such as membrane fouling (polysaccharides, polyphenols), selectivity–permeation flux trade-offs, and energy costs are assessed. By balancing tradition with innovation, RO/NF technologies offer transformative potential for meeting health and sustainability goals in beverage industries. However, gaps in standardization, sensory consistency, and cost-effectiveness necessitate targeted research to optimize industrial adoption and consumer acceptance. Full article

Background/Objectives: This paper aims to assess the unhealthy dietary habits and sedentary behaviours among adolescents and young adults simultaneously. Methods: The sample consists of 682 participants aged 10–24 years old (58.9% males and 68.2% aged 15–19 years old), recruited for the ALIMA (ALImentazione Multiculturale negli Adolescenti) study in both secondary schools and youth aggregation centres, located in Rome. The study utilizes structured questionnaires to gather data on anthropometric measurements, eating habits, alcohol consumption, physical activity, and sedentary behaviours. Data are analyzed by using the Pearson chi-squared test. Results: Excessive screen time (>3 h/day) and inadequate dietary habits (insufficient consumption of fruit, vegetables, and legumes, skipping breakfast, and a high consumption of sugar-sweetened beverages and alcohol) are observed in the total sample, but mainly in older adolescents aged 15–19 years old. The adherence to the MD, measured via the KIDMED index, is significantly different among the three age groups (p = 0.001); 47.8% of the sample has a medium adherence, while among those with a low adherence, the highest percentage is represented by older adolescents (39.8%); those with foreign-born parents have healthier diets, whereas lower parental education correlates with unhealthy behaviours. Conclusions: These results highlight the need for targeted interventions tailored to adolescents and young adults to encourage healthier lifestyle choices. Full article

Background/Objectives: Fermented foods promote digestion and may help prevent chronic diseases. However, studies on their relationship with health indicators in Korea remain limited. This study aimed to identify fermented food consumption patterns among middle-aged Korean adults and to analyze their association with high-sensitivity C-reactive protein (hs-CRP) and chronic disease. Methods: This study analyzed 7111 adults aged 40–64 years from the 6th–7th Korea National Health and Nutrition Examination Survey (KNHANES, 2015–2018). hs-CRP values were categorized as <1 mg/L, 1–3 mg/L, and ≥3 mg/L. Latent profile analysis (LPA) classified fermented foods into 10 categories using Mplus 8.11, with LMR-LRT significance and entropy ≥ 0.7 determining the number of classes. Logistic regression analysis using SPSS 29.0 was conducted to calculate odds ratios (ORs) and 95% confidence intervals (CIs) for hs-CRP and chronic disease (p < 0.05). Results: LPA identified four consumption patterns: <Low Fermented-Food Pattern> (LFP), <Fermented Alcohol- and Beverage-Centered Pattern> (FABP), <Fermented Dairy-Centered Pattern> (FDP), and <Fermented Grain-Centered Pattern> (FGP). hs-CRP was highest in LFP (1.0 ± 1.2 mg/L), followed by FABP (0.8 ± 1.1 mg/L) and FDP and FGP (0.9 ± 1.2 mg/L) (p < 0.001). Compared to LFP, FGP had ORs of 0.810 (95% CI: 0.690–0.950, p < 0.005) for hypertension and 0.586 (95% CI: 0.459–0.747, p < 0.001) for diabetes. For dyslipidemia, ORs were 0.832 (95% CI: 0.720–0.962, p < 0.005) for FABP and 0.832 (95% CI: 0.719–0.962, p < 0.005) for FDP. Conclusions: This study classified fermented food consumption patterns and analyzed their association with hs-CRP and chronic disease. FGP and FDP showed lower inflammation and reduced odds of hypertension, diabetes, and dyslipidemia compared to LFP. These findings highlight the potential of healthy fermented food consumption patterns to support inflammation control and chronic disease prevention. Full article

This study is the first to examine the diet and nutritional status of the adult vegetarian and semi-vegetarian population in Serbia, using data from the EFSA EU Menu Food Consumption Survey 2017–2022. The survey included 314 participants (63 vegans, 192 lacto-ovo vegetarians, 50 pescatarians, and 9 flexitarians), aged 18–74 years (166 women and 148 men, with no gender differences in dietary patterns) across all regions of Serbia. Collected data included anthropometrics (BMI) and intake of energy, macronutrients, and specific food groups (assessed through two 24 h dietary recalls). The study revealed multiple nutritional shortcomings across all three dietary patterns. The most significant was an insufficient protein intake (especially among vegans, but also among non-vegans), connected with an unsatisfactory protein quantity, quality, and availability in plant sources. There was also a high fat intake (particularly from omega-6 and trans-fats-rich sources), especially among non-vegans (but also among vegans), while the intake of omega-3 sources was low. Non-vegans consumed less carbohydrates, fiber, vegetables, and fruit, but more sweets, beverages, and alcohol. Our findings highlight the need for improved nutritional education of vegetarians/semi-vegetarians in Serbia and the development of national food system-based guidelines for this population. Full article

The rising prevalence of hyperuricemia and gout, driven by dietary purine intake, has intensified demand for healthier alcoholic beverages. Beer, a major contributor to exogenous purines, poses significant health risks despite its cultural and economic importance. This review systematically analyzes purine sources in beer, metabolic pathways leading to uric acid production, and cutting-edge strategies for purine reduction. We evaluate physical adsorption, enzymatic degradation, microbial fermentation, and yeast metabolic engineering, highlighting their efficacy and limitations in industrial applications. Challenges such as flavor preservation, regulatory compliance, and scalability are critically discussed. By integrating multidisciplinary approaches ranging from synthetic biology to process optimization, this work provides a roadmap for developing commercially viable low-purine beers, bridging the gap between public health priorities and brewing industry innovation. Full article

Winemakers are increasingly adopting partial or total dealcoholisation of wines and alcoholic beverages in response to market trends and the impact of climate change on wine alcohol content. In this study, the patented very low-temperature vacuum wine distillation technology known as GoLo was used to dealcoholise various types of red, white, and rosé wines from different Spanish designations of origin (DOs) in order to examine changes in oenological parameters as pH, sulphites, total acidity, volatile acidity, and sugars and the perceptible differences among a set of wines through sensory analysis and the chemical analysis related, such as turbidity, total phenolic content, and a profile of volatile compounds. The results indicate that there is an increase in the overall polyphenol content in the range of 8 to 12%, turbidity increased in the range of 13 to 70%, and sulphites decreasing in the range of 20 to 40%. The aroma profile also reveals that the most volatile and less soluble compounds—such as esters (reduction between 60% and 96%), terpenes (reduction between 45% and 80%), and aldehydes (reduction between 86% and 95%)—are lost in varying percentages during the dealcoholisation process, depending on the type of wine. Meanwhile, other more soluble compounds like phenols and acids undergo transformations during GoLo’s dealcoholisation process, leading to an increase in their concentrations up to more than 90% in the case of phenols in white wines or 35% for acids in red wines. Full article

Beer, the most popular alcoholic beverage, poses health risks for individuals with gout and hyperuricemia due to its high purine content. Herein, we identified a novel purine nucleoside phosphorylase (AbPNP) from the edible mushroom Agaricus bisporus and heterologously expressed it in Pichia pastoris. The recombinant AbPNP exhibited optimal activity at 60 °C and pH 7.0, retaining >80% activity at pH 6.0–9.0 and >85% activity after 3 h at ≤60 °C. Kinetic analysis revealed high catalytic efficiency (kcat/Km = 2.02 × 10⁶ s⁻¹⋅M⁻¹) toward inosine, with strong resistance to metal ions except for Co²⁺ and Cu²⁺. The application of AbPNP (1.0–5.0 U/mL) during wort saccharification reduced purine nucleosides by 33.54% (from 151.53 to 100.65 mg/L) while increasing yeast utilization of free purine bases. The resulting beer showed improved fermentation performance (alcohol content increased by 3.6%) without compromising flavor profiles. This study provides the food-grade enzymatic strategy for low-purine beer production, leveraging the GRAS status of both A. bisporus and P. pastoris. Full article

Since 1975, when per capita wine consumption in Spain peaked, the continued decline in domestic consumption has led to significant changes in the wine sector, particularly in production and marketing strategies. By 2005, the industry had shifted towards mass exportation, focusing on bulk wine, as domestic demand fell. Since then, Spain has become the leading global producer of bulk wine by volume. However, emerging consumer trends, prioritizing health, sustainability, and low-alcohol beverages, present both challenges and opportunities for the sector. This study explores the potential for Spain’s bulk wine industry to adapt by transitioning to dealcoholized wines, leveraging recent technological advancements and aligning with shifting consumer preferences. In this context, we examined what additional factors might influence the transition of Spanish wine producers to dealcoholized wine production and how consumer acceptance of these products in Spain compares to other international markets. In this context, it is crucial to examine what additional factors might influence the transition of Spanish wine producers to dealcoholized wine production and how consumer acceptance of these products in Spain compares to other international markets. Data collection was conducted through dual surveys targeting both consumers and producers, administered in December 2024 via the Sobrelias.com platform, with a total of 602 responses (387 from consumers and 215 from producers). Statistical analyses, including ANOVA and PERMANOVA, were performed to assess the influence of variables such as health-conscious consumption habits, demographic segmentation (age groups), and gender on market dynamics. To date, no prior academic research has addressed this specific intersection of bulk wine exports and consumer trends, highlighting the novelty of this study. A multiple linear regression model quantified the potential for market growth based on these factors. Full article