Search Results (80)

Arbuscular mycorrhizal fungi (AMF) taxa, glomalin protein, and hyphal density are potential indicators of soil functionality of temperate grasslands in marginal environments subject to grazing over the years. This study evaluated how the AMF community composition, glomalin protein, and hyphal density vary in response to grazing intensity (low or high) and seasonality (spring and autumn) in sodic soils of Argentinian temperate grasslands. The AMF community was dominated by Glomeraceae species. Funneliformis geosporus and Glomus brohultii were the most abundant in both seasons and all grasslands. No AMF species were associated with a particular grazing intensity. However, Entrophospora etunicata, Glomus fuegianum, Septoglomus constrictum, and Acaulospora sp. occurred only in spring, and no species were exclusive to autumn. Hyphal density was highest in grasslands with low grazing intensity and can be considered an indicator of soil functionality. Glomalin protein was the highest in spring in all grasslands. The lower grazing intensity in grasslands with poor livestock control showed no changes in AMF diversity. The AMF community showed high adaptation to soil conditions, indicating high resilience. We concluded that longer periods of controlled grazing management are needed to improve soil conditions and, consequently, change the AMF species composition. Full article

Intestinal microbial eukaryotic parasites represent a significant public and veterinary health burden, especially in low- and middle-income countries, yet their transmission dynamics at the human–animal–environment interface remain poorly characterized in certain countries. This study investigated the prevalence and genetic diversity of key microbial eukaryotes, including Cryptosporidium spp., Giardia duodenalis, Blastocystis spp., Entamoeba histolytica, and Enterocytozoon bieneusi, in a rural village in Iraq. Samples collected from humans (n = 50), livestock (sheep and goats, n = 50), water (n = 20), and soil (n = 20) were analysed using microscopy and molecular methods (qPCR and nested PCR). Blastocystis spp. (78% animals, 16% humans, 45% soil, 5% water) and Cryptosporidium spp. (26% animals, 12% humans, 5% soil, 15% water) were the most frequently found microeukaryotes using either microscopy and/or molecular detection. Molecular methods identified Cryptosporidium parvum in humans and sheep, hinting at zoonotic transmission potential. Enterocytozoon bieneusi and Giardia were also found. Cryptosporidium ubiquitum and E. bieneusi genotypes BEB6 and COS-I, respectively, were detected exclusively in sheep, suggesting roles as potential reservoirs. Blastocystis ST1 was detected in humans, while ST4 and ST10 occurred in sheep. Notably, molecular detection rates of Blastocystis were much lower than those of microscopy. Entamoeba histolytica was not detected. The detection of the same organisms in humans, animals and the environment suggest zoonotic and environmental transmission pathways, which warrant further investigation using the One Health approach. Full article

Understanding the complex and multidimensional nature of poverty is essential for designing effective and targeted policy interventions in rural Ethiopia. This study examined the determinants of multidimensional poverty in Bilate River Basin in South Ethiopia, employing cross-sectional household survey data collected in 2024. A total of 359 households were selected using a multistage sampling technique, ensuring representation across agro-ecological and socio-economic zones. The analysis applied the Generalized Ordered Logit (GOLOGIT) model to categorize households into four mutually exclusive poverty statuses: non-poor, vulnerable, poor, and extremely poor. The results reveal that age, dependency ratio, education level, livestock and ox ownership, access to information and credit, health status, and grazing land access significantly influence poverty status. Higher dependency ratios and poor health substantially increase the likelihood of extreme poverty, while livestock ownership and access to grazing land reduce it. Notably, credit use and access to information typically considered poverty reducing were associated with increased extreme poverty risks, likely due to poor financial literacy and exposure to misinformation. These findings underscored the multidimensional and dynamic nature of poverty, driven by both structural and behavioral factors. Policy implications point to the importance of integrated interventions that promote education, health, financial literacy, and access to productive assets to ensure sustainable poverty reduction and improved rural livelihoods in Ethiopia. Full article

Bovine trypanosomiasis, caused by Trypanosoma vivax, affects livestock productivity and is increasingly being reported in South America. This study aimed to detect and characterize Trypanosoma spp. infections, with a focus on T. vivax, in cattle from two distinct agroecological regions of central Argentina: a dairy-producing plain, located in the Espinal ecoregion, and a riparian zone, dedicated to beef production, located in the Delta and Islands of Paraná ecoregion. A total of 220 blood samples were collected from nine cattle farms and analyzed using real-time PCR, melting curve analysis, and the sequencing of 18S rRNA gene fragments. Trypanosoma vivax was detected at low prevalence (2.73%), exclusively in dairy cattle. In contrast, the prevalence of Trypanosoma theileri was much higher (10.91%), and it was found mainly in beef cattle from the riparian region. Phylogenetic analyses confirmed the species identity in all sequenced samples. No trypanosomes were observed by microscopy, and none of the animals showed clinical signs. The results indicate a differential distribution of T. vivax and T. theileri between regions and production systems. Although the study initially focused on T. vivax, the detection of T. theileri highlights the need to consider multiple Trypanosoma species in epidemiological surveys. This study contributes new data on the occurrence of bovine trypanosomes in central Argentina under extensive and semi-intensive management systems. Full article

Emerging tick-borne infections pose growing public health threats, causing global disease burdens and economic losses. In this study, tick-borne pathogens were detected in ticks collected from ruminants in 19 sites of Khyber Pakhtunkhwa Province, Pakistan, between 2023 and 2024. A total of 989 ticks, belonging to five species, i.e., Hyalomma marginatum, Rhipicephalus microplus, Rhipicephalus sanguineus, Rhipicephalus haemaphysaloides, and Haemaphysalis bispinosa, were tested by specific PCR followed by Sanger sequencing. In total, fourteen pathogens including two Anaplasma species, three Ehrlichia species, three Rickettsia species, one Babesia species, and five Theileria species were identified, with an overall infection rate of 20.2% (95% CI: 17.7–22.7%). Phylogenetic analyses revealed two undefined Ehrlichia species: Candidatus Ehrlichia hyalommae was exclusively detected in Hy. marginatum ticks, while Candidatus Ehrlichia rhipicephalis was only found in R. microplus. Additionally, an undefined Rickettsia, provisionally named Candidatus Rickettsia pakistanensis, was identified, which is phylogenetically close to R. sibirica in North Asia and R. africae in Africa, suggesting its potential pathogenicity to humans. Although coinfections of two pathogens were observed, the coinfection rates were quite low. The findings revealed a significant diversity of tick-borne pathogens in Pakistani ticks, which may pose risks to livestock and humans. Full article

Dermatophytes are commonly encountered pathogens in clinical practice causing superficial infections of the skin, hair, and nails. These pathogens are often found on animals such as livestock (e.g., cattle, rabbits) and pets (e.g., cats, hedgehogs) that can lead to spillover infections in human populations. Here, we reviewed published reports (2009–2024) of dermatophyte infections in animals and in humans with a history of animal contact. A literature search was completed in October 2024 using PubMed, Embase (Ovid), and Web of Science (Core Collection), which identified 250 articles. Generally, dermatophytes tend to infect younger animals with long hair and exhibit a species-specific host range. Microsporum canis was the most commonly reported species—linked to cats—that can cause tinea capitis, especially concerning the development of kerion in children. Trichophyton verrucosum is strongly associated with cattle. The Trichophyton mentagrophytes complex shows a diverse range of animal hosts, with rabbits being most frequently reported; however, T. mentagrophytes var. erinacei is almost exclusively isolated from hedgehogs, and T. mentagrophytes var. benhamiae is more commonly found on rodents (e.g., guinea pigs). Lastly, the geophilic Nannizia gypsea has been isolated from both dogs and cats. Managing dermatophyte zoonoses is an ongoing challenge, as healthcare providers may empirically treat with corticosteroids or antibacterial agents due to its atypical inflammatory appearance. Evidence of in vitro resistance against griseofulvin and fluconazole has been documented in multiple zoonotic dermatophyte species. Resistance development against terbinafine and itraconazole is also a possibility, although the number of reports is scarce. Under the principles of the One Health approach, research on human fungal diseases should take animal and environmental factors into account. A renewed call for increased testing efforts is warranted. Full article

This research developed a predictive model using NeuralProphet to estimate energy consumption in the ventilation system of a dairy cattle farm. The necessity for energy management in livestock farming has increased due to the growing energy demands associated with climate control systems. Approximately two years of historical energy consumption data, collected through a smart monitoring system deployed on the farm, were utilized as the primary input for the NeuralProphet model to predict long-term trends and seasonal variations. The computational results demonstrated satisfactory performance, achieving a coefficient of determination (R²) of 0.85 and a mean absolute error (MAE) of 27.47 kWh. The model effectively captured general trends and seasonal patterns, providing valuable insights into energy usage under existing operational conditions. However, short-term fluctuations were less accurately predicted due to the exclusion of exogenous climatic variables, such as temperature and humidity. The proposed model demonstrated superiority over traditional approaches in its capacity to forecast long-term energy demand, providing critical support for energy management and strategic decision-making in dairy farm operations. Full article

Precision agriculture (PA) is a farm management data-driven technology that enhances production with efficient resource usage. Existing PA methods rely on data processing, highlighting the need for a portable computing device for real-time, infield decisions. Raspberry Pi, a cost-effective multi-OS single-board computer, addresses this gap. However, information on Raspberry Pi’s use in PA remains limited. This review consolidates details on Raspberry Pi versions, sensors, devices, algorithm deployment, and PA applications. A systematic literature review of three academic databases (Scopus, Web of Science, IEEE Xplore) yielded 84 (as of 22 November 2024) articles based on four research questions and screening criteria (exclusion and inclusion). Narrative synthesis and subgroup analysis were used to synthesize the results. Findings suggest Raspberry Pi can be a central unit to control sensors, enabling cost-effective automated decision support for PA, particularly in plant disease detection, site-specific weed management, plant phenotyping, biomass estimation, and irrigation systems. Despite focusing on these areas, further research is essential on other PA applications such as livestock monitoring, UAV-based applications, and farm management software. Additionally, Raspberry Pi can be used as a valuable learning tool for students, researchers, and farmers and can promote PA adoption globally, helping stakeholders realize its potential. Full article

Trypanosoma vivax displays distinct mitochondrial genomes: intact in Africa and degraded in South America. This finding, formerly reported from four isolates, suggested an association between mitochondrial mutations and adaptation to exclusive mechanical transmission outside Africa, circumventing mitochondrial activity essential for cyclical transmission by tsetse flies. Here, we characterized the mitochondrial ND7 and COIII genes in 25 South American isolates of T. vivax from livestock from Argentina, Brazil, Colombia, Ecuador, and Venezuela, as well as in 11 African isolates from Mozambique, Ethiopia, Uganda, Nigeria, and Burkina Faso from tsetse flies, antelopes, and cattle. Phylogenetic inference supported the lineages TVV (Trypanosoma vivax vivax) and TVL (Trypanosoma vivax-like), predominant in South America/West Africa and East Africa, respectively. Both ND7 and COIII genes were intact in all African TVV and TVL isolates and, for the first time, in 10 South American isolates from Brazil. Remaining South American isolates exhibited some degree of gene erosion, including ~142 bp deletion at the 3′ end of ND7 disclosed in Venezuela and Colombia, ~163 bp deletion at the 3′ end of ND7 in Brazil and Argentina, and ~750 bp deletion from the 5′ end of ND7 to the 3′ end of COIII in Venezuela. Comprehensive analyses of whole mitochondrial genomes from additional South American and African isolates are crucial for formulating reliable hypotheses regarding the origin, absence, and rapid mitochondrial degradation observed in South American T. vivax. Full article

Bovine viral diarrhea virus (BVDV), a pestivirus in the family Flaviviridae, is a major livestock pathogen. Horizontal transmission leads to acute transient infections via the oronasal route, whereas vertical transmission might lead to the birth of immunotolerant, persistently infected animals. In both cases, BVDV exerts an immunosuppressive effect, predisposing infected animals to secondary infections. E^rns, an immunomodulatory viral protein, is present on the envelope of the virus and is released as a soluble protein. In this form, it is taken up by cells and, with its RNase activity, degrades single- and double-stranded (ds) RNA, thus preventing activation of the host’s interferon system. Here, we show that E^rns of the pestiviruses BVDV and Bungowannah virus effectively inhibit dsRNA-induced IFN synthesis in well-differentiated airway epithelial cells cultured at the air–liquid interface. This activity was observed independently of the side of entry, apical or basolateral, of the pseudostratified, polarized cell layer. Virus infection was successful from both surfaces but was inefficient, requiring several days of incubation. Virus release was almost exclusively restricted to the apical side. This confirms that primary, well-differentiated respiratory epithelial cells cultured at the air–liquid interface are an appropriate model to study viral infection and innate immunotolerance in the bovine respiratory tract. Furthermore, evidence is presented that E^rns might contribute to the immunosuppressive effect observed after BVDV infections, especially in persistently infected animals. Full article

Epidemiological and eradication trial evidence indicates that Helicobacter pylori, a major causative factor in peptic ulcer and gastric cancer, is a driver of the hypokinesia of Parkinson’s disease (PD). Psychological (cognitive impairment, depression and anxiety) and gastrointestinal (peptic ulceration and constipation) PD features can precede the symptomatic onset of motor features by decades. We hypothesise that the non-H. pylori Helicobacters (NHPH), which have farm, companion and wild animals as their main hosts, can have a role in PD aetiopathogenesis. In those occupationally at risk of NHPH infection, we address whether there is increased mortality with PD, or depression or suicide. Our systematic review gave evidence that occupational exposure to animals/their products is associated with excess mortality with PD. Indeed, whilst livestock farming increased the risk, crop farming decreased it. Moreover, excess mortality from non-Hodgkin lymphoma in livestock farmers is compatible with NHPH being causal. Our scoping review showed that farmers, veterinarians and abattoir workers have an increased risk of depression and suicide; whether their depression is associated with being down the pathway to PD and/or the presence of Helicobacter infection needs investigation. Regarding Helicobacter species specificity, the link between the presence of NHPH in gastric biopsy and PD was described using a ureA polymerase chain reaction (PCR) assay, targeting the most-commonly named NHPH, H. suis. We describe its redesign and optimisation as a probe-based PCR, confirming the exclusion of H. pylori but not H. suis specificity (additionally identifying 6 species of a 22-NHPH-species panel). The exploration of the zoonotic hypothesis requires a non-invasive pan-Helicobacter PCR screen, allowing the detection and molecular grouping of Helicobacter species. Full article

Background: The circulation of Leptospira has been linked to various occupational activities globally. This study investigated the seroprevalence of Leptospira spp. in rodents and livestock (cattle and goats) in three settlements/villages involved in agriculture, livestock keeping, and mixed agriculture and livestock in the Kilombero district, Tanzania. Methods: Data were collected during the wet and dry seasons. A total of 179 rodents were live-captured from selected habitats. Livestock samples were collected from 80 cattle in a livestock settlement and 120 goats from both livestock and mixed agricultural–livestock settlements. The microscopic agglutination test was utilized to identify Leptospira serovars. Results: The seroprevalence of Leptospira spp. was 17.3% in rodents (21.7% in Mastomys natalensis and 3.9% in Rattus rattus) and 8.3% in livestock (13.5% in cattle and 12.6% in goats). The prevalence among rodents and livestock differed between settlements (p = 0.01). A higher prevalence was observed among rodents in the agricultural settlement relative to the other settlements. A higher prevalence of antibodies in livestock was observed in the livestock settlement compared with the mixed agricultural–livestock settlement. The Leptospira serovars Sokoine (serogroup Icterohaemorrhagiae) and Hebdomadis (serogroup Hebdomadis) were detected in both rodents and livestock. The serovars Hardjo (serogroup Sejroe) and Gripothyphosa (serogroup Gripothyphosa) were found exclusively in cattle, whereas the serovars Pomona (serogroup Pomona) and Lora (serogroup Australis) were identified in rodents. Leptospira antibodies were found to be elevated during the rainy season compared with the dry season (p = 0.05) in all settlements, with the exception of rodents in the mixed agricultural–livestock settlement. Conclusions: This study demonstrates the presence of anti-Leptospira antibodies in rodents and livestock related to occupational activities in human settlements. It further demonstrates that wild animals (rodents) and livestock are reservoirs of Leptospira and are important in the epidemiology of leptospirosis. Management and control strategies should target both rodents and livestock. Full article

Cottonseed meal (CSM) has emerged as an alternative source of protein-based poultry feed because of the increasing cost and scarcity of soybean meal. However, it contains toxic gossypol, which restricts its use. This systematic review examines solid-state fermented CSM’s impact on broiler chickens, focusing on growth performance, carcass traits, and blood parameters. To identify relevant papers, a comprehensive search of Web of Science, Scopus, and PubMed was conducted. In accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) methodology, peer-reviewed experimental articles published in English up to September 2023 were screened. A total of 60 articles were evaluated over four stages, resulting in 9 articles that met the predefined inclusion and exclusion criteria. The reduction in gossypol content was effective with bacteria like Bacillus subtilis, Aspergillus oryzae, and Aspergillus niger. The content of free gossypol in CSM is significantly reduced after solid-state fermentation using various microorganisms. Fermented CSM increased the body weight gain and feed intake of broilers, while maintaining feed efficiency, and improved growth performance. Fermented CSM included in diets positively affected carcass traits, including increased carcass weight and breast muscle development. It also resulted in decreased fat content and enhanced immune organ development. An improvement in total protein, albumin, and immunoglobulin levels was observed in blood biochemical parameters, suggesting an improved humoral immune response. Depending on the microorganisms used and their concentrations, fermented CSM also had positive effects on cholesterol, triglycerides, and LDL levels. Solid-state fermented CSM can be an effective and sustainable source of protein for broiler chickens. A future research focus should be on long-term effects, underlying mechanisms, economic feasibility, and scalability. In addition, it could be used in novel feed formulations and other livestock species to further enhance animal agriculture. Full article

The sustainability concept has acquired a variety of meanings according to different currents of thought and the context in which it is used. The aim of this research was to characterize the perception of cattle producers, extension specialists, and chief officers regarding sustainable cattle ranching (SCR) in an area where tropical cattle ranching is practiced. A survey was carried out among cattle producers using stratified random sampling and among extension specialists and chief officers chosen using the snowball technique. The perception of SCR was determined under two contexts: (1) the idea or interpretation and (2) the assessment (positive or negative). The idea that producers have about SCR is centered on healthy and adequate food, higher production, and better marketing of livestock. The perception of extension specialists and officials is based on greater business vision and environmental conservation. Producers oriented their perception towards the economic (48.4%) and environmental (21.3%) dimensions, similar to extension specialists (52.2% and 47.8%, respectively), while chief officers related it more exclusively to the environmental dimension (71.4%). It is concluded that the overall perception of SCR is positive, but its conceptualization is differentiated according to the role the individuals play in the activity, their academic training, and their expectations. Full article

This study centers on examining the carbon/nitrogen (C/N) ratio and metal levels in various batches of manure and their potential impact on biogas production through anaerobic fermentation. A novel aspect of this research involves the utilization of nine distinct batches sourced exclusively from livestock manure found in Romanian farms, without mixing with other potential substrates. At present, the farms are not harvesting manure for energy, but they are keen to invest in biogas production in the future as a necessary step towards renewable energy in a circular economy and a bio-waste management model. As a general conclusion that is resulting, it is shown that both the C/N ratio and the content of heavy metals in animal manure must be known when dealing with the animal manure fermentation process, especially when aiming for biogas production. The C/N ratio in the analyzed samples ranges from 6.7 to 30.2. While the ideal ratio is often considered 20–30, good methane production can occur outside this range, as seen in Sample B (small pig farm), with a C/N ratio of 13.8, proving the highest methane output. This shows that the C/N ratio is important but not the only factor influencing biogas generation. The metal content in the manure samples is similar to other studies, with potassium (K) ranging from 1.64% to 8.96%. Calcium (Ca) and K are the main metals found, posing little concern. The variation in values is linked to feed recipes. Monitoring heavy metals is crucial not only for biogas production but also for the safe use of animal manure as fertilizer, as soil contamination limits must be continuously supervised. The results are also valuable for the management of waste used as fertilizer in agricultural fields in accordance with EU law. Full article