Search Results (8)

Potato (Solanum tuberosum L.) is an important vegetable crop that plays a pivotal role in the world, especially given its potential to feed the world population and to act as the major staple food in many developing countries. Every year, significant crop loss is caused by viral diseases due to a lack of effective agrochemical treatments, since only transmission by insect vectors can be combated with the use of insecticides, and this has been an important factor hindering potato production. With the rapid development of molecular biology and plant genetic engineering technology, transgenic approaches and non-transgenic techniques (RNA interference and CRISPR-cas9) have been effectively employed to improve potato protection against devastating viruses. Moreover, the availability of viral sequences, potato genome sequences, and host immune mechanisms has remarkably facilitated potato genetic engineering. In this study, we summarize the progress of antiviral strategies applied in potato through engineering either virus-derived or plant-derived genes. These recent molecular insights into engineering approaches provide the necessary framework to develop viral resistance in potato in order to provide durable and broad-spectrum protection against important viral diseases of solanaceous crops. Full article

Tetrastigma hemsleyanum Diels et Gilg, known as a “plant antibiotic”, possesses several attractive properties including anti-inflammatory, anti-tumor, and antioxidant effects, with its efficacy being attributed to flavonoids. However, the flavonoid biosynthesis of T. hemsleyanum has rarely been studied. In this study, we investigated the flavonoid metabolism of T. hemsleyanum through metabolome analysis and transcriptome sequencing. The metabolomic results showed differences in the flavonoids of the leaves and root tubers of T. hemsleyanum. A total of 22 flavonoids was detected, and the concentrations of most flavonoids in the leaves were higher than those in the root tubers. Transcriptome analysis revealed that differentially expressed genes (DEGs) in the leaves and root tubers were enriched in photosynthesis-antenna proteins. Pearson correlation analysis indicated that the expression levels of chalcone isomerase (CHI) and UDP-glycose flavonoid glycosyltransferase (UFGT) were highly correlated with the concentrations of most flavonoids. Further, this study found that the photosynthesis-antenna proteins essentially contributed to the difference in the flavonoids in T. hemsleyanum. The gene expressions and concentrations of the total flavonoids of leaves and root tubers in Hangzhou, Jinhua, Lishui, and Taizhou in Zhejiang Province, China, showed that CHI (CL6715.Contig1_All, Unigene19431_All, CL921.Contig4_All) and UFGT (CL11556.Contig3_All, CL11775.Contig1_All) were the potential key genes of accumulation of most flavonoids in T. hemsleyanum. Full article

The degree of informatization of coal mine safety management is becoming higher and higher, and a large amount of information is generated in this process. How to convert the existing information into useful data for risk control has become a challenge. To solve this challenge, this paper studies the mathematical model of coal mine risk early warning in China based on data mining. Firstly, the coal mine risk data was comprehensively analyzed to provide basic data for the risk prediction model of data mining. Then, the adaptive neuro-fuzzy inference system (ANFIS) was optimized twice to build the coal mine risk prediction model. By optimizing the calculation method of the control chart, the coal mine risk early warning system was proposed. Finally, based on the coal mine risk early warning model, the software platform was developed and applied to coal mines in China to control the risks at all levels. The results show that the error of the optimized ANFIS was reduced by 66%, and the early warning error was reduced by 57%. This study aimed to provide implementation methods and tools for coal mine risk management and control, and data collected has reference significance for other enterprises. Full article

The Chaoyang profile represents a rare multi-period, continuous and complete sequence of aeolian paleo-deposits with a stable sedimentary origin and multi-stage paleoclimatic cycles. Benchmark profiles including soil types at different pedogenic stages can be used for the recognition and classification of paleosols and paleoclimate reconstruction. The loess–paleosol sequence benchmark profile (LBP) is also helpful in comparing the results of paleoenvironment reconstruction from different ecological regions. In this study, a loess–paleosol profile derived from thick loess in Chaoyang city of Liaoning province, Northeast China, was investigated as a well-preserved LBP that included various paleosol types. To determine the nature and origin of the Chaoyang profile, the geographic, stratigraphic and morphological characteristics were described in the field. Bulk samples from 42 horizons were collected for chemical and physical analysis, and sub-sampling of 946 samples at 2 cm intervals from the surface to the bottom were taken to measure grain size distributions and magnetic susceptibility. Results showed that the 19.85 m thick loess–paleosol profile had been continuously deposited since 423 ka BP. The upper part (0–195 cm), or UPP, was predominantly of aeolian loess deposition origin but was mixed with water-reworked materials from a nearby secondary loess source. The middle part (195–228 cm), or MIP, was also indirectly affected by the water-reworking process through the leaching of materials from the overlying UPP. The lower part (228–1985 cm), or LOP, was characterized by four reddish stratigraphic layers interbedded with five yellowish ones, indicating several types of paleosols developed under different ecological environments. The multi-stage paleoclimatic cycles as evidenced by morphological and physical characteristics as well as age dating and magnetic susceptibility correlated well with the Lingtai section and LR04 benthic δ¹⁸O. Because of these attributes, the Chaoyang profile can be deemed as a benchmark loess–paleosol profile for the recognition and classification of paleosols and paleoclimate reconstruction in Northeast China. The differences in morphological and physical properties between paleosols and loess suggest different soil fertility and agronomic properties and need further studies to assess their functionality with climate fluctuation. Full article

In order to determine the rational width of coal pillars and study the surrounding rock control technology of gob side entry driving with a narrow coal pillar, this paper first calculates the width of narrow coal pillar according to limit equilibrium theory; after that, the lateral support pressure and plastic zone development of the goaf is analyzed by numerical simulation to determine the rational width of reserved coal pillar; finally, through the ring breaking deformation regulation of surrounding rock of the gob side entry, the deformation and failure characteristics of the gob-side roadway during excavation and the influence of mining on the deformation and failure of the gob-side roadway are analyzed. The research results show that, combined with theoretical analysis and numerical simulation, the width of narrow coal pillar is decided to be 10 m; the deformation of the coal pillar side of the gob side roadway is much bigger than the roof subsidence, the deformation of the solid coal wall and the floor deformation; after the bolt support design of the gob side roadway, the deformation and damage of the gob side roadway during the driving period is small; during mining, the deformation of the narrow coal pillar wall is the key factor to determine the stability of the gob roadway; under the bolt support scheme, the overall deformation and failure of surrounding rock of the goaf roadway is small, and the control effect of the surrounding rock of the goaf roadway is good. Full article

This article explores the Buddhist ritual and architectural conventions that were incorporated into the Chinese funeral architecture during the medieval period from the 3rd to the 13th centuries. A careful observation of some key types of sacred architectural forms from ancient East Asia, for instance, pagoda, lingtai, and hunping, reviews fundamental similarities in their form and structure. Applying translation theory rather than the influence and Sinicization model to analyze the impact of Buddhism on Chinese funeral architecture, this article offers a comparative study of the historical contexts from which certain architectural types and imageries were produced. It argues that there was an intertwined mutual translation of formal and ritual conventions between Buddhist and Chinese funeral architecture, which had played a significant role in the formations of both architectural traditions in Medieval China. Full article

Drug combinations have been the hotspot of the pharmaceutical industry, but the promising applications are limited by the unmet solubility and low bioavailability. In this work, novel cocrystals, consisting of two antithrombotic drugs with poor solubility and low bioavailability in vivo, namely, apixaban (Apx) and quercetin (Que), were developed to discover a potential method to improve the poor solubility and internal absorption of the drug combination. Compared with Apx, the dissolution behavior of Apx–Que (1:1) and Apx–Que–2ACN (1:1:2) was enhanced significantly, while the physical mixture of the chemicals failed to exhibit the advantages. The dissolution improvements of Apx–Que–2ACN could be explained by the fact that the solid dispersion-like structure and column-shaped cage of Que accelerated the access of the solvent to the inner layer of Apx. The fracture of the hydrogen bonds of Apx, which was the joint of the adjacent Que chains, facilitated the break-up of the structures. Besides, the bioavailability of Apx–Que was increased compared with the physical mixture and Apx, and Apx–Que remained stable in high temperature and illumination conditions. Therefore, a drug–drug cocrystal of two antithrombotic agents with poor solubility was developed, which exhibited greatly improved solubility, bioavailability and superior stability, indicating a novel method to overcome the shortages of drug combination. Full article

Bexarotene (BEX), a specific retinoic acid X receptor (RXR) agonist granted by Food and Drug Administration (FDA) approval for the clinical treatment of T cell lymphoma, has now been found to exert pharmacological effects in the nervous system, with low bioavailability and poor cerebral distribution limiting its application in treatment on neurological disorders. Pharmaceutical co-crystal was a helpful method to improve the bioavailability and tissue distribution of active pharmaceutical ingredients (APIs). Here, 2bexarotene-ligustrazine (2BEX-LIG), a novel co-crystal system of BEX and ligustrazine (LIG) of which with BEX is an API, was constructed with satisfactory stability and enhanced solubility. The pharmacokinetics characteristics of BEX were detected, and the results showed that the absolute bioavailability and the cerebral concentration of BEX in rats administrated with 2BEX-LIG were enhanced from 22.89% to 42.86% and increased by 3.4-fold, respectively, compared with those in rats administrated an equivalent of BEX. Hence, our present study indicated that the novel co-crystal of 2BEX-LIG contributed to improving BEX oral bioavailability and cerebral distribution, thereby providing significant advantages for clinical application of brain tumors and other neurological diseases. Full article