Search Results (81)

Biofilm-mediated antimicrobial resistance remains a significant challenge for healthcare and patient safety. Currently, there are gaps in standardised methods for assessing antimicrobials against biofilm formations such as (1) assessment of initial bacterial attachment inhibition, as well as (2) assessment of antimicrobial compounds against both the external biofilm mass and biofilm-embedded metabolically active bacteria. The aim of this study is to address these gaps by combining several anti-biofilm techniques. In the procedure96-well anti-biofilm assessments were performed using plate well and lid peg growth surfaces so as to determine the effects of bioactive compounds (silver nitrate (AgNO₃), nisin, chitosan and zinc oxide nanopowder (ZnO)) on biofilm growth inhibition, formed biofilm reduction and bacterial attachment inhibition. These studies focused on the initial attachment stage against in vitro biofilms of P. aeruginosa and S. aureus. Effects were measured against biofilm mass using Crystal Violet (CV) staining, while embedded bacteria metabolic activity was measured using Resazurin. AgNO₃ exhibited significant inhibition and reduction against P. aeruginosa at all stages of biofilm development (p < 0.0001). AgNO₃ showed significant results against S. aureus during biofilm development and against the embedded, metabolically active population of established biofilms (p < 0.0001). Nisin showed significant inhibition against S. aureus biofilm populations (p < 0.0001). Chitosan showed significant increases in S. aureus biofilm formations following exposure, during initial attachment (p < 0.02), during biofilm growth (p < 0.0001) and against formed biofilm populations (p < 0.0001). ZnO showed significant increases during initial attachment exposure (p < 0.0001), but also exhibited growth inhibition (p < 0.0001) and biofilm reduction (p < 0.0001). Although variance in anti-biofilm efficacy was evident depending upon treatment used, Gram-staining phenotype and test growth surfaces, this combinational method offers potential for high throughput screening and for evaluating pipeline bioactives isolated from different environments for biofilm prevention, inhibition and removal. Additionally, this approach will help elucidate the relationship between bacteria of interest and biofilm mitigation. Full article

The rise in electric vehicles (EVs) with lithium-ion batteries supports net-zero goals, but the increasing demand will inevitably generate more battery waste. Current pack designs often rely on permanent joining techniques, which hinder disassembly and thereby limit serviceability, reuse and recycling. A critical challenge is the removal of the battery lid, typically bonded to the pack frame with sealant adhesives. In the absence of design for disassembly requirements for OEMs, this study investigates a novel debonding strategy focused on the lid-to-frame bonding. A silane-based adhesive commonly used in battery packs is first characterised under tensile, shear and mode I conditions to establish the baseline performance in the range of flexible adhesive properties. Herein, a heat-activated primer is introduced as a debondable interfacial layer between the adhesive and the substrate. Upon activation at 150 °C, the primer significantly reduces adhesion, around 98% of the initial joint strength, enabling room temperature debonding. The primer demonstrates strong compatibility with epoxy and polyurethane adhesives, but its performance with silane-based systems still needs to be improved in terms of the primer’s compatibility with silane-based adhesives. Finally, a small-scale testing apparatus is developed to evaluate primer effectiveness in the disassembly of battery lids. This approach represents a promising step toward more serviceable, recyclable and sustainable battery systems. Full article

Nowadays various coupling strategies have been developed to combine the strengths of different numerical methods in computational fluid dynamics (CFD), among which the coupled algorithm of the lattice Boltzmann-finite volume method (LBM-FVM) has gained widespread attention. However, research on parallel optimization of LBM-FVM coupled solvers remains limited, mostly focused on independent solvers. In this work, we proposed a flexible framework and optimization schemes to explore the coordinated balance of accuracy-efficiency-hardware adaptability. First, we designed a processor layout strategy to address load imbalance and communication redundancy in the coupled solver. We then developed several parallelization techniques, including LBM restructuring, data reuse, and SIMD optimization for targeted kernels on the most advanced architecture of the Sunway series in China, namely SW26010P heterogeneous many-core processors, which provide hardware architectural advantages well suited for large-scale parallel computational fluid dynamics. Finally, the accuracy of the LBM-FVM coupling simulations was validated through benchmark simulations of 2D/3D lid-driven cavity flow. The results show that our LBM-FVM coupling solver can accurately capture flow characteristics, with vortex structures consistent with experimental data. Additionally, we achieved a 152× speedup for the LBM solver and a 126× speedup for the coupled simulation compared to the standalone FVM simulation on the New Sunway supercomputer system. Our approach marks a milestone in the field of LBM implementations and provides a promising future for coupled algorithms in CFD. Full article

Background: The development of calcium silicate materials and new techniques have resulted in significant clinical benefits in endodontics and microapical surgery. The objective of this investigation was to analyze the percentage of dentinal tubule penetration of two retrograde obturation techniques in microapical surgery, namely the conventional technique and the lid technique. Methods: 60 single-root human teeth were selected, which were divided into two groups (n = 30). These teeth were subjected to an endodontic procedure using the single-cone technique. They were prepared with apicoectomy and 3 mm apical retrocavity and then obturated using two retrograde obturation techniques with bioceramic materials: TotalFill RRM fast set Putty^® (RRM) using the conventional technique and TotalFill BC Sealer HiFlow^® (HiFlow) and RRM using the lid technique. The teeth were selected and evaluated using 1 mm portions in the apical third. In each case, the images were obtained using a Leica TCS SP8 Confocal Microscope (CLSM). The extent of penetration into the dentinal tubule regions was measured using AutoCad^®. Results: Statistical analyses were performed using the Levene test (p ≤ 0.05) and Student’s t-test (p ≤ 0.05). Analysis of the penetration area of calcium silicate materials into the dentinal tubules revealed that the relative penetration percentages were higher when using the conventional technique with the RRM than the lid technique with RRM + HiFlow in the apical third evaluated. Conclusion: The conventional technique yields significantly better outcomes, showing statistically significant differences in the percentage of penetration into the intratubular area compared to the lid technique. Full article

The objective of this study was to evaluate the feasibility and efficacy of the ultrasound-guided thoracolumbar erector spinae plane (TL-ESP) block in standing horses. A total of 24 injections (n = 12) were performed at the L1 level using either 0.1 mL/kg of saline solution (SS group) or 2% lidocaine (LID group). The block feasibility was assessed based on needle visualization and injection time, while efficacy was evaluated through craniocaudal and dorsoventral (DV) spread using the pinprick technique over 270 min. Desensitization was observed at least once in 100% of horses in the LID group and in 75% in the SS group (p = 0.001). However, in the SS group, desensitization was primarily limited to the Th18 metamer at the 2 cm DV position, with a shorter duration compared to the LID group. The block onset occurred at 22.5 (11.25–60) min in the LID group and at 5 (5–30) min in the SS group (p = 0.069). The number of affected metamers was significantly higher in the LID group (2 [1–3]) compared to the SS group (1 [1–2.25], p = 0.014). At the 2 cm DV point, the end of the block effect occurred at 135 (120–210) min in the LID group and at 60 (3.75–60) min in the SS group (p = 0.001). Needle visualization was excellent in 95.8% of cases, and the mean injection time was 2.5 (2–3) min. These findings confirm that the TL-ESP block is a feasible technique in standing horses. However, its effect is predominantly localized to dorsal dermatomes with a limited ventral spread. Future studies evaluating larger volumes and multiple injection sites are warranted to enhance its clinical applicability. Full article

Background: The reconstruction of extensive full-thickness lower eyelid defects constitutes a challenge for plastic surgeons. Various techniques have been described to cater to patients’ specific defect needs, with the aim of achieving the best results. Materials and Methods: We performed a retrospective observational study assessing our experience with a combination of a single-stage procedure consisting of a hard palate graft and a Fricke flap for patients with complex lower lid resections undergoing immediate total reconstruction at our institution. Clinical data, histological type and results, size of tumor, recurrences, and post-operative complications were collected to evaluate outcomes. A Visual Analogue 10-point scale was administered to all patients to assess esthetic and functional outcomes. Results: Seven lower lid reconstructions were performed, with all patients receiving immediate reconstruction. The age of the patients ranged from 55 to 82. Five skin cancers were located on the right side and three on the left side. In all cases, histological diagnosis was non-melanoma skin cancers. The mean size of the tumor was 1.7 × 1.7. In all patients, negative surgical margins were obtained. All patients underwent 24-month follow up. No immediate complication from surgery was recorded within the first 30 days. During follow-up, lower lid ectropion was observed in one patient due to the development of a retracting scar. No local cancer recurrence or nodal metastasis were detected until 2 years follow-up. In only one case, adjuvant therapy was required. The aesthetic results were deemed satisfactory by all patients. Conclusions: According to our experience, the combination of a Fricke flap and hard palate graft is an excellent option for total lower eyelid reconstruction, with low morbidity and favorable outcomes, even in elderly and frail patients where satisfactory results were achieved in a single-stage procedure and short operative times. Full article

This study addresses the growing inadequacies of traditional architectural concepts and techniques in stormwater management amid the increasing frequency of extreme weather events, particularly in densely built urban micro-spaces. To tackle these challenges, we propose an integrated theoretical and practical framework applied to a case study of a small-scale urban public space in Chang’an District, Shijiazhuang City, Hebei Province, covering an area of about 2.15 hectares in North China. The framework combines Best Management Practices Planning (BMP-P) with Low Impact Development Design (LID-D). The framework optimizes sub-catchment delineation, strategically locates drainage outlets, and configures network layouts to reduce runoff path lengths, thereby reducing total runoff volume, enhancing drainage capacity, and alleviating surface water accumulation, which, in turn, informs the parametric design of LID facilities. In the BMP-P phase, four source-control measures were developed based on runoff control and stormwater retention: adjusting terrain slopes, adding or removing curbs and facilities, redistributing infiltration areas, and adjusting drainage outlet and piping layouts. By shortening runoff paths and reducing potential waterlogging areas, these measures effectively reduced total runoff volume (Trv) by 31.5% to 35.7% and peak runoff volume (Prv) by 19.4% to 32.4%. Moreover, by remodeling the stormwater network with a different layout, larger pipe diameters, and substantially increased network capacity, the total discharge (Tdv) increased by 1.8% to 50.2%, and the peak discharge rate (Pdr) increased by 100% to 550%, thus minimizing surface flooding. In the LID-D phase, we developed a Grasshopper-based parametric design program for the layout and design of LID facilities. This approach significantly reduces interdisciplinary communication costs and enhances urban planning efficiency. By integrating BMP and LID strategies, the proposed framework offers a flexible, rapid, and efficient solution for achieving resilient stormwater management in the context of urban micro-renewal. Full article

In recent years, the urgent need to mitigate stormwater runoff and address urban waterlogging has garnered significant attention. Low Impact Development (LID) has emerged as a promising strategy for managing urban runoff sustainably. However, the vast array of potential LID layout combinations presents challenges in quantifying their effectiveness and often results in high construction costs. To address these issues, this study proposes a simulation-optimization framework that integrates the Storm Water Management Model (SWMM) with advanced optimization techniques to minimize both runoff volume and costs. The framework incorporates random variations in rainfall intensity within the basin, ensuring robustness under diverse climatic conditions. By leveraging a multi-objective scatter search algorithm, this research optimizes LID layouts to achieve effective stormwater management. The algorithm is further enhanced by two local search techniques—namely, the ‘cost–benefit’ local search and path-relinking local search—which significantly improve computational efficiency. Comparative analysis reveals that the proposed algorithm outperforms the widely used NSGA-II (Non-dominated Sorting Genetic Algorithm II), reducing computation time by an average of 8.89%, 16.98%, 1.72%, 3.85%, and 1.23% across various scenarios. The results demonstrate the method’s effectiveness in achieving optimal LID configurations under variable rainfall intensities, highlighting its practical applicability for urban flood management. This research contributes to advancing urban sponge city initiatives by providing a scalable, efficient, and scientifically grounded solution for sustainable urban water management. The proposed framework is expected to support decision-makers in designing cost-effective and resilient stormwater management systems, paving the way for more sustainable urban development. Full article

Background: This study introduces a novel surgical approach, the Marginal Approach for Releasing the lid with Closure Handling technique (MARCH technique), a single-step sparing tissue technique, for the reconstruction of medium to large full-thickness lower eyelid defects and its outcomes. Methods: The research considers a single-centre case series with a description of the MARCH technique. Patients with a full-thickness medium to large lower eyelid defect underwent this technique, which combines inferior cantholysis, splitting of lamellae and island or advancement flaps. Demographic data, lid defect size, histology and postoperative outcomes were collected. Results: The surgical procedure was performed in fifteen patients (nine males and six females). The mean age was 73.9 years (range 48–95 years old). Local anaesthesia was used in 86.7% of cases. The mean defect size was 68.7% (range 50% to 79%) of the lid length. All patients presented good results with no significant complications. Conclusions: The MARCH technique seems to be an excellent first-line approach in reconstructing medium to large full-thickness lower eyelid defects. Its single-step approach, tissue-sparing and minimally aggressive nature and ability to potentially guide eyelash positioning and restore the lacrimal system with a more anatomical position make it a promising option. Enrolling more patients and a longer follow-up would provide a better assessment. Full article

Background: Ectropion is a common eyelid problem and is defined as eversion of the eyelid margin and typically involves the lower eyelid. The main acquired causes of ectropion include involutional, cicatricial, paralytic, and mechanical. A severe manifestation of ectropion is tarsal ectropion, where much of the tarsal conjunctiva is visible, often with keratinization of the conjunctiva. causes. Common techniques for ectropion repair include horizontal tightening of the lid with lateral tarsal strip or Bick procedure, lateral tarsorraphy, inverting sutures and the sub-orbicularis oculi fat lift. However, all surgical techniques are prone to ectropion recurrence. We review the techniques for ectropion repair and describe a novel adjunctive technique called the superotemporal skin transposition (STS), which is well suited for patients with recurrent or tarsal ectropion. Methods: The STS is combined with a lateral tarsal strip or Bick procedure. For the STS, all of the anterior lamellae of the lateral lower lid is retained. The posterior lamellae is sutured to the lateral orbital tubercle. A triangular bed of skin is excised superotemporally, and the lower lid anterior lamellae is transposed and secured with multiple sutures. The STS can be combined with inverting sutures, or skin graft for cicatricial cases. Results: We used the STS with Bick procedure and optional inverting sutures on 23 patients, 4 of whom required bilateral ectropion repair. At 1–6 month followup all patients achieved satisfactory outcomes with a well-positioned eyelid and improved symptoms. The STS had more lateral cutaneous scarring than with a Bick procedure alone, but patients did not find this objectionable. No reoperations were required. Conclusion: The STS is a straightforward and useful adjunct for patients with severe, recurrent or tarsal ectropion. Further studies are needed to determine the long-term efficacy of this technique. Full article

The study of entropy generation in thermal non-equilibrium (TNE) states has significant implications for optimizing thermal management systems and understanding heat transfer mechanisms in permeable media. This study investigates the entropy properties in a thermal non-equilibrium (TNE) state within double-lid-driven enclosures filled with a permeable medium. Unlike the temperature equilibrium state, the entropy approach is described by two equations: one for the irreversibility of the mixture phase and one for the irreversibility of the medium phase. High mixed convection is considered due to the motion of the non-facing edges (left-side and upper edges). Four cases based on the direction of motion are examined: Case 1, where the left-side and top edges move in the negative and positive directions of the Y- and X-axes, respectively; Case 2, where the upper and left-side edges move in the negative and positive directions of the X- and Y-axes, respectively; and Cases 3 and 4, where the edges move in the positive and negative directions of the respective axes. Heat generation within the flow domain is considered for both the suspension and medium phases. The governing system is solved numerically using finite volume techniques with the SIMPLER algorithm. The obtained data are used to predict key quantities, such as the heat transfer rate, under the influence of major factors using an effective artificial neural network (ANN) analysis. The main findings show that the solid phase entropy is higher in Case 3 compared to the other cases. Additionally, Case 2 results in a minimum solid phase Nusselt coefficient at the center of the active boundary. Full article

The main aim of this study is to achieve the numerical solution for the Navier–Stokes equations for incompressible, non-turbulent, and subsonic fluid flows with some Gaussian physical uncertainties. The higher-order stochastic finite volume method (SFVM), implemented according to the iterative generalized stochastic perturbation technique and the Monte Carlo scheme, are engaged for this purpose. It is implemented with the aid of the polynomial bases for the pressure–velocity–temperature (PVT) solutions, for which the weighted least squares method (WLSM) algorithm is applicable. The deterministic problem is solved using the freeware OpenFVM, the computer algebra software MAPLE 2019 is employed for the LSM local fittings, and the resulting probabilistic quantities are computed. The first two probabilistic moments, as well as the Shannon entropy spatial distributions, are determined with this apparatus and visualized in the FEPlot software. This approach is validated using the 2D heat conduction benchmark test and then applied for the probabilistic version of the 3D coupled lid-driven cavity flow analysis. Such an implementation of the SFVM is applied to model the 2D lid-driven cavity flow problem for statistically homogeneous fluid with limited uncertainty in its viscosity and heat conductivity. Further numerical extension of this technique is seen in an application of the artificial neural networks, where polynomial approximation may be replaced automatically by some optimal, and not necessarily polynomial, bases. Full article

The study area is a densely populated residential zone located in central Mexico, characterized by a semi-arid climate and diverse land uses, including domestic, commercial, and services. In the study area, water demand is assessed based on land use requirements set by national and local regulations, and an urban configuration pinpoints spaces suitable for rainwater harvesting for self-consumption. This research proposes indicators based on urban, demographic, and hydrological parameters to assess the effectiveness of low-impact development (LID) techniques, such as rooftop rainwater harvesting, aimed at reducing water scarcity in a residential area vulnerable to water shortages in a semi-arid city in the Global South. Additionally, hydrological design conditions are defined to estimate the potential volumes of recoverable water. Indicators for infiltrated water, available water for consumption, runoff water, and sufficient water are established. This framework enables the development of strategies to mitigate water scarcity and improve water management in the area. Full article

Background: Involutional lower eyelid ectropion is a common disorder of the elderly population. Several surgical approaches have been described in the literature to address the multifactorial nature of this condition, each targeting different factors contributing to its development. Nevertheless, no single procedure has proven to be superior to the others. This study aims to assess the safety and effectiveness of a new 3-step combined technique in treating involutional ectropion. Methods: The surgical technique consists of a combination of lateral lid-shortening with removal of a base-up triangle (modified Bick procedure), lower eyelid skin release, and transposition of a laterally based myocutaneous flap from the upper eyelid. A retrospective chart review was conducted for all patients treated with this procedure at our institution between 2012 and 2023 was performed. Self-reported patient satisfaction with functional and esthetic outcomes was evaluated three months after surgery. Results: A total of thirty-six patients (forty-five eyelids) were included in the study, with a mean follow-up period of 22 months (range 3–144). Minor surgical revision was performed in one case for early wound dehiscence following premature stitches removal. The overall success rate was 93.2%, with one case of undercorrection and two cases of recurrence recorded at three years postoperatively. No additional complications or recurrences were observed during the follow-up period. Conclusions: The 3-step combined procedure demonstrated high efficacy and safety, offering excellent functional and esthetic outcomes. This approach provides a reliable solution for treating involutional ectropion, making it a valuable addition to the surgical options for this condition. Full article

Background/Objectives: Eversion carotid endarterectomy (CEA) in awake patients is performed using cervical plexus blocks (CPBs) with or without carotid artery sheath infiltration (CASI) under ultrasound guidance. Although adequacy of anesthesia (AoA) guidance monitors nociception/antinociception balance, its impact on intraoperative analgesia quality and perioperative outcomes in awake CEA remains unexplored. Existing literature lacks evidence on whether AoA-guided anesthesia enhances clinical outcomes over standard techniques. This study aimed to assess the role of AoA guidance in improving intraoperative analgesia and perioperative outcomes in patients undergoing CEA with CPBs alone or with CASI compared to standard practice. Methods: A randomized controlled trial included 184 patients divided into three groups: CPBs with intravenous rescue fentanyl (IRF) and lidocaine (LID) guided by hemodynamic observation (C group), AoA-guided IRF and LID (AoA group), and AoA-guided IRF, LID, and CASI (AoA-CASI group). Primary outcomes included perioperative adverse events, and secondary outcomes assessed rescue medication demand and hemodynamic stability. Results: Analysis of 172 patients revealed no significant differences between groups in perioperative adverse events or hemodynamic parameters (p > 0.05). However, the AoA-CASI group demonstrated significantly reduced IRF and LID usage compared to the C and AoA groups (p < 0.001). No significant advantage was observed between the AoA and C groups regarding adverse events (p = 0.1). Conclusions: AoA-guided anesthesia with or without CASI does not significantly reduce perioperative adverse events or improve hemodynamic stability in awake CEA. Clinical implications suggest that focusing on surgical technique optimization may yield greater benefits in reducing adverse events compared to advanced anesthetic monitoring. Further studies are warranted to explore alternative approaches to enhance clinical outcomes. Full article