Search Results (35)

The integration of IT services is a critical challenge for public organizations that seek to modernize their operational ecosystems and strengthen mission-oriented processes. In the field of fiscal oversight, supreme audit institutions (SAIs) increasingly require systematized and interoperable service architectures to ensure transparency, accountability, and effective public resource control. However, existing literature reveals persistent gaps concerning how service integration models can be deployed and validated within complex government environments. This study describes an enterprise architecture-driven service integration model designed and evaluated within the Office of the General Comptroller of the Republic of Colombia (Contraloría General de la República, CGR). The study tests the hypothesis that an Enterprise Architecture-driven integration model provides the necessary structural coupling to align technical IT performance with the legal requirements of fiscal oversight, which is an alignment that typically does not appear in generic governance frameworks. The methodological approach followed in this study combines an IT service management maturity assessment, process analysis, architecture repository review, and iterative validation sessions with institutional stakeholders. The model integrates ITILv4 (Information Technology Infrastructure Library), TOGAF (The Open Group Architecture Framework), COBIT (Control Objectives for Information and Related Technologies), and ISO20000 into a coherent framework tailored to the operational and regulatory requirements of an SAI. Results show that the proposed model reduces service fragmentation, improves process standardization, strengthens information governance, and enables a unified service catalog aligned with fiscal oversight functions. The empirical validation demonstrates measurable improvements in service delivery, transparency, and organizational responsiveness. The study contributes to the field of applied system innovation by: (i) providing an integration model, which is scientifically grounded and evidence-based, (ii) demonstrating how hybrid governance and architecture frameworks can be adapted to complex public-sector environments, and (iii) offering a replicable approach for SAIs that seek to modernize their technological service ecosystems through enterprise architecture principles. Future research directions are also discussed to provide guidelines to advance integrated governance and digital transformation in oversight institutions. Full article

Ethiopic manuscript studies have become a rapidly expanding field in recent decades. However, most research has focused on cataloging and textual analysis. This study examines the material traces of original addenda, patterns of deterioration, and desecration of indigenous conservation ethics. A combination of codicological and paleographic methods was used. This approach is vital for documenting historical features, understanding the context of use, and informing conservation efforts. The research involved assessing twenty-eight physical manuscripts from two collections in Addis Ababa, Ethiopia. Additionally, twenty-seven digital copies from the Endangered Archives Program, the Hill Museum & Manuscript Library, and the University of Cambridge Digital Library repositories were consulted. The findings revealed original features like holes, repairs, and scribal corrections. Damage such as tears, creases, dirt, fading, erasures, and recent writing was also identified. These results reveal the material history of the manuscripts. Furthermore, both domestic and international stakeholders have adversely affected these manuscripts through erasure, dispossession, and appropriation. This study proposes ethical guidelines for recent additions to the manuscripts and for preserving the original addendum. It also underscores the necessity for additional material research, enhancements in conservation practices, and efforts to raise awareness. Full article

Enzyme replacement therapy is one of the main alternatives in the treatment of mucopolysaccharidosis. Conventionally, this therapy is administered in an outpatient setting, but this can be carried out at home, where the patient feels most comfortable. However, there is still a need for guidance on how to implement the use of this therapy in the home setting. This study aims to present the protocol of a scoping review that aims to identify and map the main existing guidance for the implementation of home-based enzyme replacement therapy in children and adolescents with mucopolysaccharidosis. The review protocol was developed based on the methodological guidance proposed by Peters and the PRISMA-P checklist and was registered on the Open Science Framework platform, available at OSF.IO/QUSB6. The searches will be carried out in the following databases: CINAHL, Cochrane Library, Embase, LILACS, MEDLINE, Science Direct, Scopus, and Web of Science. Gray literature will be searched in the CAPES/Brazilian Theses and Dissertations Catalog, the Brazilian Ministry of Health website, Google Scholar, the National Institute for Health and Care Excellence, and the WHO IRIS. Articles will be selected according to predefined eligibility criteria and extracted using a standardized collection form. The results of this review are expected to provide valuable insights for researchers regarding the main guidance for the implementation of home-based ERT in children and adolescents with mucopolysaccharidosis. Full article

Francisella tularensis is a pathogenic bacterium and the causative agent of the disease tularemia. Because of the virulence of this bacterium and the potential for weaponization, the Centers for Disease Control and Prevention (CDC) has classified F. tularensis as a Category A Bioterrorism Agent. Therefore, the need for new treatments for tularemia is critical. In this work, we screened a cataloged library of natural extracts to identify those that inhibit the growth of F. tularensis only during infection of THP-1 monocyte cells. One of the most promising extracts identified in this screen was derived from Foeniculum vulgare (fennel). Using bioassay-guided fractionation, the fennel extract was fractionated, and the bioactive compound was isolated and structurally elucidated as the phenylpropanoid dillapiole. We subsequently confirmed that dillapiole alone could limit the replication of F. tularensis in infected THP-1 cells, but not outside of this infection model. Investigations on host responses suggested that dillapiole was not substantially augmenting the immunity of these THP-1 cells. We then investigated the potential virulence modulation activity of dillapiole. To test this hypothesis, RNA-seq analysis was carried out on F. tularensis bacteria that were treated with dillapiole. This showed that dillapiole caused a significant downregulation of genes controlled by the transcriptional regulators MglA and SspA, including those encoded in the Francisella pathogenicity island. Western blotting validated these findings as both IglA and IglC expression was diminished in F. tularensis LVS bacteria treated with dillapiole. Because dillapiole dampens the virulence gene expression of F. tularensis, we concluded that this compound has potential to be used as a novel therapeutic for tularemia with a unique mechanism of action. Full article

Introduction: Although Brazil includes industrialized regions, such as the Southeast, it also has underdeveloped areas with poor sanitation, such as the North and Northeast, resembling regions in Africa and Asia where HEV is endemic. In Brazil, HEV is suspected to occur mainly as a zoonosis. Given the wide variation in HEV prevalence across the five regions, a scoping review was conducted to systematically evaluate its prevalence and circulating genotypes. Aim: To investigate the epidemiological characteristics of HEV in Brazil, including modes of transmission, by reviewing genotyping studies in humans and swine. Methods: This scoping review followed the methodological framework of the Joanna Briggs Institute (JBI) and the PRISMA-ScR checklist. Gray literature was retrieved from Google Scholar, the Brazilian Digital Library of Theses and Dissertations, and the Thesis and Dissertation Catalog of the Coordination for the Improvement of Higher Education Personnel. Searches were performed in June and July 2025 in MEDLINE and LILACS. The evidence on HEV epidemiology in Brazil was mapped using the Population, Concept, and Context strategy. Results: Among 57 studies on HEV prevalence in Brazil, 45 (78.9%) involved humans and 12 (21.1%) involved swine. IgG prevalence ranged from 0.5% in the North to 59.4% in the South. IgM prevalence was lowest in the Northeast (0.1%) and highest in the North (16.3%). In swine, HEV was detected in all regions, with variation in sample types, husbandry practices, and prevalence. Genotyping revealed exclusively HEV-3 in all regions where analysis was performed. Conclusions: HEV infection is present throughout Brazil, with higher prevalence in the South and Southeast. The circulating genotype is HEV-3, and transmission is likely linked to swine breeding and consumption. Full article

Cocoa (Theobroma cacao L.) is a major agricultural commodity, essential for the global chocolate industry and the livelihoods of millions of farmers. However, viral diseases pose a significant threat to cocoa production, with Badnavirus species causing severe losses in Africa. Despite its economic importance, the overall virome of T. cacao remains poorly characterized, limiting our understanding of viral diversity and potential disease interactions. This study aims to assess the cocoa-associated virome by analyzing 109 publicly available RNA-seq libraries from nine BioProjects, covering diverse conditions and geographic regions. We implemented a comprehensive bioinformatics pipeline integrating multiple viral sequence enrichment steps, a hybrid assembly strategy using different assemblers, and sequence similarity searches against NCBI non-redundant databases. Our approach identified ten putative novel viruses associated with the cocoa microbiome and a novel Badnavirus species. These findings provide new insights into the viral landscape of T. cacao, characterizing the diversity of cacao-associated viruses and their potential ecological roles. Expanding the catalog of viruses associated with cocoa plants not only enhances our understanding of plant–virus–microbiome interactions but also contributes to the development of more effective disease surveillance and management strategies, ultimately supporting sustainable cocoa production. Full article

Herbert D. Athearn (1923–2011) was an avid student of freshwater mollusks. He named his private shell collection “The Museum of Fluviatile Mollusks”, which was meticulously organized at his residence. This collection was curated to current museum standards with detailed labels, all lots with catalog numbers, and all unionoid valves with catalog numbers written in India ink. Specimens’ collecting dates span between 1850 and 2005, with 23,344 cataloged lots containing over 3000 lots of imperiled and extinct taxa. All data for each of the lots are handwritten in paper catalogs. Many lots contain growth series from the smallest juveniles to the largest specimens seen. He traded extensively with collectors worldwide, obtaining specimens from 84 countries. This collection was donated to the North Carolina Museum of Natural Sciences in 2007. To date, 64 percent of this collection has been databased using a relational database, totaling 589,995 specimens. The collection consists of bivalves, primarily Unionidae, Margaritiferidae, and Sphaeriidae, as well as gastropods. There are 73 families represented, with the greatest abundance found in freshwater Pleuroceridae. The Athearn collection donation included his correspondence, his library, field notes, and USGS topographic maps with marked field localities. Full article

With the rapid advancement of aerospace technology, the maneuverability of spacecraft has increasingly improved, creating a pressing demand for star sensors with a high attitude update rate and high precision. Star identification, as the most complex and time-consuming algorithm of star sensors, faces stringent requirements for enhanced identification speed and an enhanced identification rate. Furthermore, as the space environment is becoming more complex, the need for star sensors with heightened detection sensitivity is growing to facilitate real-time and accurate alerts for various non-cooperative targets, which has led to a sharp increase in the number of high-magnitude navigation stars in the star catalog, significantly impeding the speed and rate of star identification. Traditional methods are no longer adequate to meet the current demand for star sensors with high identification speed and a high identification rate. Addressing these challenges, a voting-based star identification algorithm using a partitioned star catalog is proposed. Initially, a uniform partitioning method for the star catalog is introduced. Building on this, a navigation feature library using partitioned catalog neighborhoods as a basic unit is constructed. During star identification, a method based on a voting decision is employed for feature matching in the basic unit. Compared to conventional methods, the proposed algorithm significantly simplifies the navigation feature library and narrows the retrieval region during star identification, markedly enhancing identification speed while effectively reducing the probability of redundant and false matching. The performance of the proposed algorithm is validated through a simulation experiment and nighttime star observation experiment. Experimental results indicate an average identification rate of 99.760% and an average identification time of 8.861 milliseconds, exhibiting high robustness against position errors, magnitude errors, and false stars. The proposed algorithm presents a clear advantage over other common star identification methods, meeting the current requirement for star sensors with high star identification speed and a high identification rate. Full article

Betalains, which contain nitrogen and are water soluble, are the pigments responsible for many traits of plants and biological activities in different organisms that do not produce them. To better annotate and identify betalains using a spectral library and fingerprint, a database catalog of 140 known betalains (112 betacyanins and 28 betaxanthins) was made in this work to simplify betalain identification in mass spectrometry analysis. Fragmented peaks obtained using MassFrontier, along with chemical structures and protonated precursor ions for each betalain, were added to the database. Product ions made in MS/MS and multistage MS analyses of betanin, beetroot extract, and red pitaya extract revealed the fingerprint of betalains, distinctive ions of betacyanin, betacyanin derivatives such as decarboxylated and dehydrogenated betacyanins, and betaxanthins. A distinctive ion with m/z 211.07 was found in betaxanthins. By using the fingerprint of betalains in the analysis of red pitaya extracts, the catalog of betalains in red pitaya was expanded to 86 (31 betacyanins, 36 betacyanin derivatives, and 19 betaxanthins). Four unknown betalains were detected to have the fingerprint of betalains, but further research will aid in revealing the complete structure. Taken together, we envisage that the further use of the fingerprint of betalains will increase the annotation coverage of identified molecules in studies related to revealing the biological function of betalains or making technologies based on these natural colorants. Full article

Many non-natural amino acids can be incorporated by biological systems into coded functional peptides and proteins. For such incorporations to be effective, they must not only be compatible with the desired function but also evade various biochemical error-checking mechanisms. The underlying molecular mechanisms are complex, and this problem has been approached previously largely by expert perception of isomer compatibility, followed by empirical study. However, the number of amino acids that might be incorporable by the biological coding machinery may be too large to survey efficiently using such an intuitive approach. We introduce here a workflow for searching real and computed non-natural amino acid libraries for biosimilar amino acids which may be incorporable into coded proteins with minimal unintended disturbance of function. This workflow was also applied to molecules which have been previously benchmarked for their compatibility with the biological translation apparatus, as well as commercial catalogs. We report the results of scoring their contents based on fingerprint similarity via Tanimoto coefficients. These similarity scoring methods reveal candidate amino acids which could be substitutable into modern proteins. Our analysis discovers some already-implemented substitutions, but also suggests many novel ones. Full article

Fragment screening directly on protein crystals has been applied using AnalytiCon’s collection of intermediates that have been utilized to generate libraries of larger synthetic natural product-like molecules. The fragments with well-balanced physicochemical properties show an impressively high hit rate for a screen using the aspartic protease endothiapepsin. The subsequent validation and expansion of the discovered fragment hits benefits from AnalytiCon’s comprehensive library design. Since the screened fragments are intermediates that share a common core with larger and closely related analogs with modulated substitution patterns, they allow for the retrieval of off-the-shelf follow-up compounds, which enable the development of design strategies for fragment optimization. A promising bicyclic core scaffold found in several fragment hits could be validated by selecting a set of enlarged follow-up compounds. Due to unexpected changes in binding mode and no significant improvement in ligand efficiency, this series was quickly deemed unsuitable and therefore discontinued. The structures of follow-up compounds of two other fragments helped to evaluate a putative fusion of two overlapping fragment hits. A design concept on how to fuse the two fragments could be proposed and helps to plan a suitable substitution pattern and promising central bridging element. Full article

This paper presents a detailed study on the main parameters and performance evaluation of microjet engines, at take-off regime and at various engine working regimes, based on thermodynamic analysis of a particular engine data library, from different engine manufacturers such as JetCat and AMT Netherlands. The studied engines have the same spool design but different thrust classes ranging from 97 to 1569 N. The particular data library includes engine specifications from catalogs or data sheets as well as our own experimental data from the JetCat P80 microjet engine, obtained using the ET 796 Jet Turbine Module, a complete testing facility for gas turbine education purposes. Various ratios and differences between certain engine main parameters and performances are studied in order to calculate values through which the analyses can be performed. Even if the engines have different thrust classes, the study examines if there are close values of the ratios and differences of parameters, that can be defined as reference parameters through which the engine performance can be compared and evaluated. Full article

Configurators have recently emerged as essential tools in the construction industry to enable builders to offer a wide range of customizable designs. Due to significant challenges in information integration between construction suppliers and clients, existing configurator systems often lack crucial usability and supply chain information, presenting barriers to wider adoption among residential communities, especially in single-family residence development that requires a high degree of customization. To address this challenge in the design and construction supply chain, this study presents a lightweight cloud-based modular home configuration methodology as a robust unified platform solution to integrate parametric design options with a certified kit-of-parts library to meet local design codes. The configurator prototype developed under this framework seamlessly integrates essential design and supply chain information by leveraging (1) a generative layout design with pre-approved blueprints, (2) a knowledge-based recommender system to link the design process with certified material catalogs, and (3) a user-friendly web interface to present possible designs. The implementation of a single-family housing design adhering to the building codes in the British Columbia Province of Canada illustrates the benefits of the proposed configurator functionalities and efficient supplier data integration. Lightweight and automated, the proposed configurator has substantial potential to be scaled and adopted across different communities. Full article

In Spain, the public National Health Service provides care to Spaniards and other residents and is tailored for a decentralized state of autonomies. Each Autonomous Community has legislative capacity in its organization and management. We study the case of the collaboration between private hospitals and the public health service in La Rioja, an Autonomous Community of Spain located in the North of the Iberian Peninsula, due to the importance that this relationship has in health systems, in general. We applied the case study method as a methodological tool in a long-term local study. The interpretation was carried out within a national context, which allows us to understand its meaning and the historical keys to hospital development in this region. Primary sources have been reviewed (mainly reports, catalogs, and censuses of hospitals from the Ministry of Health and the Government of La Rioja) and other secondary sources, located in archives, libraries, Institute of Rioja Studies, and Department of Health. The hospital system in La Rioja was characterized by a predominance of public beds compared with private ones, although there has been a growing trend in the number of private beds from 2013 onwards due to the incorporation of health and social care convalescent hospitals (two). La Rioja has been promoting public–private collaboration (seen as a strategic alliance) and focusing on agreements in the socio-health space, particularly using the management service agreement and the concession of work formulas. The development of the public health service in La Rioja, from 1986 to 2019, has been determined by a progressive lower dependence on specialized hospitals from other health services of neighboring Autonomous Communities and by a mixed public–private hospital system. Full article

The use of radar technology for non-contact measurement of vital parameters is increasingly being examined in scientific studies. Based on a systematic literature search in the PubMed, German National Library, Austrian Library Network (Union Catalog), Swiss National Library and Common Library Network databases, the accuracy of heart rate and/or respiratory rate measurements by means of radar technology was analyzed. In 37% of the included studies on the measurement of the respiratory rate and in 48% of those on the measurement of the heart rate, the maximum deviation was 5%. For a tolerated deviation of 10%, the corresponding percentages were 85% and 87%, respectively. However, the quantitative comparability of the results available in the current literature is very limited due to a variety of variables. The elimination of the problem of confounding variables and the continuation of the tendency to focus on the algorithm applied will continue to constitute a central topic of radar-based vital parameter measurement. Promising fields of application of research can be found in particular in areas that require non-contact measurements. This includes infection events, emergency medicine, disaster situations and major catastrophic incidents. Full article