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Search Results (524)

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25 pages, 4107 KB  
Article
Simple and Affordable Vision-Based Detection of Seedling Deficiencies to Relieve Labor Shortages in Small-Scale Cruciferous Nurseries
by Po-Jui Su, Tse-Min Chen and Jung-Jeng Su
Agriculture 2025, 15(21), 2227; https://doi.org/10.3390/agriculture15212227 (registering DOI) - 25 Oct 2025
Abstract
Labor shortages in seedling nurseries, particularly in manual inspection and replanting, hinder operational efficiency despite advancements in automation. This study aims to develop a cost-effective, GPU-free machine vision system to automate the detection of deficient seedlings in plug trays, specifically for small-scale nursery [...] Read more.
Labor shortages in seedling nurseries, particularly in manual inspection and replanting, hinder operational efficiency despite advancements in automation. This study aims to develop a cost-effective, GPU-free machine vision system to automate the detection of deficient seedlings in plug trays, specifically for small-scale nursery operations. The proposed Deficiency Detection and Replanting Positioning (DDRP) machine integrates low-cost components including an Intel RealSense Depth Camera D435, Raspberry Pi 4B, stepper motors, and a programmable logic controller (PLC). It utilizes OpenCV’s Haar cascade algorithm, HSV color space conversion, and Otsu thresholding to enable real-time image processing without GPU acceleration. The proposed Deficiency Detection and Replanting Positioning (DDRP) machine integrates low-cost components including an Intel RealSense Depth Camera D435, Raspberry Pi 4B, stepper motors, and a programmable logic controller (PLC). It utilizes OpenCV’s Haar cascade algorithm, HSV color space conversion, and Otsu thresholding to enable real-time image processing without GPU acceleration. Under controlled laboratory conditions, the DDRP-Machine achieved high detection accuracy (96.0–98.7%) and precision rates (82.14–83.78%). Benchmarking against deep-learning models such as YOLOv5x and Mask R-CNN showed comparable performance, while requiring only one-third to one-fifth of the cost and avoiding complex infrastructure. The Batch Detection (BD) mode significantly reduced processing time compared to Continuous Detection (CD), enhancing real-time applicability. The DDRP-Machine demonstrates strong potential to improve seedling inspection efficiency and reduce labor dependency in nursery operations. Its modular design and minimal hardware requirements make it a practical and scalable solution for resource-limited environments. This study offers a viable pathway for small-scale farms to adopt intelligent automation without the financial burden of high-end AI systems. Future enhancements, adaptive lighting and self-learning capabilities, will further improve field robustness and including broaden its applicability across diverse nursery conditions. Full article
(This article belongs to the Topic Digital Agriculture, Smart Farming and Crop Monitoring)
34 pages, 6555 KB  
Article
Unveiling and Evaluating Residential Satisfaction at Community and Housing Levels in China: Based on Large-Scale Surveys
by Caiqing Zhu, Zheng Ji, Sijie Liu, Hong Zhang and Juan Liu
Sustainability 2025, 17(21), 9496; https://doi.org/10.3390/su17219496 (registering DOI) - 25 Oct 2025
Abstract
In recent decades, China has witnessed remarkable growth in housing construction, yet housing-related complaints have not declined significantly, highlighting the gap between housing quality and public expectations. Against this background, this study analyzes 32,277 national surveys to unpack residential satisfaction with green-livable communities [...] Read more.
In recent decades, China has witnessed remarkable growth in housing construction, yet housing-related complaints have not declined significantly, highlighting the gap between housing quality and public expectations. Against this background, this study analyzes 32,277 national surveys to unpack residential satisfaction with green-livable communities in China. Entropy and standard-deviation weighting identified 16 priority indicators; artificial neural networks revealed weak direct influence of basic demographics on satisfaction, highlighting non-linear demand patterns. While 65–75% of respondents are satisfied with most attributes, significant city-level gaps persist—Beijing peaks near 90%, Chongqing falls below 50%. Dissatisfaction converges on three domains: infrastructure (parking, barrier-free access), building performance (leakage, noise, thermal defects) and smart systems (security, energy, health monitoring). Residents’ improvement priorities have shifted from basic shelter to health safety, smart technology, humanistic care and ecological amenities. A “basic-security + quality-upgrade” strategy is proposed: short-term repairs of common defects, medium-term smart-sustainable upgrades and long-term participatory governance. The findings not only enrich the theoretical framework of community satisfaction research but also provide practical guidance for enhancing community quality and meeting residents’ expectations in the context of China’s rapid urbanization and housing development. Full article
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28 pages, 7188 KB  
Article
A Real-World Case Study of Solar Pv Integration for Ev Charging and Residential Energy Demand in Ireland
by Mohammed Albaba, Morgan Pierce and Bülent Yeşilata
Sustainability 2025, 17(21), 9447; https://doi.org/10.3390/su17219447 - 24 Oct 2025
Viewed by 67
Abstract
The integration of residential solar photovoltaic (PV) systems with electric vehicle (EV) charging infrastructure offers significant potential for reducing carbon emissions and enhancing energy autonomy. This study presents a real-world case of a solar-powered EV charging system installed at a residential property in [...] Read more.
The integration of residential solar photovoltaic (PV) systems with electric vehicle (EV) charging infrastructure offers significant potential for reducing carbon emissions and enhancing energy autonomy. This study presents a real-world case of a solar-powered EV charging system installed at a residential property in Dublin, Ireland. Unlike prior studies that rely solely on simulation, this work covers the complete process from digital design using OpenSolar to on-site installation and performance evaluation. The system includes 16 high-efficiency solar panels (435 W each), a 4 kW hybrid inverter, a 5.3 kWh lithium-ion battery, and a smart EV charger. Real-time monitoring tools were used to collect energy performance data post-installation. The results indicate that 67% of the household’s solar energy was self-consumed, leading to a 50% reduction in electricity costs. In summer 2024, the client achieved full grid independence and received a €90 credit through feed-in tariffs. The system also enabled free EV charging and generated environmental benefits equivalent to planting 315 trees. This study provides empirical evidence supporting the practical feasibility and economic–environmental advantages of integrated PV–EV systems in temperate climates. Full article
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48 pages, 37698 KB  
Article
Transforming Construction Waste into High-Performance Alkali-Activated Paste with Microstructural and Predictive π Modelling Insights
by Israf Javed, Hamza Saeed and Abdullah Ekinci
Buildings 2025, 15(21), 3830; https://doi.org/10.3390/buildings15213830 - 23 Oct 2025
Viewed by 253
Abstract
The construction industry is among the most resource-intensive sectors, generating nearly 40% of global CO2 emissions and over two billion tonnes of construction and demolition waste (CDW) annually. This study investigates the sustainable reuse of CDW in developing binder-free alkali-activated paste (AAP) [...] Read more.
The construction industry is among the most resource-intensive sectors, generating nearly 40% of global CO2 emissions and over two billion tonnes of construction and demolition waste (CDW) annually. This study investigates the sustainable reuse of CDW in developing binder-free alkali-activated paste (AAP) using sodium hydroxide (NaOH) as an activator. Eleven formulations were prepared by varying the brick-to-total waste ratio (BW/TW: 0–1), NaOH concentrations (0–10%), and curing durations (7, 28, and 60 days). The mixes were evaluated for unconfined compressive strength (UCS), shear modulus (Go), durability (wet–dry and freeze–thaw cycles), and microstructural evolution. Results showed significant improvements in mechanical and durability properties with increased NaOH content, BW/TW ratios up to 0.9, and longer curing times. The optimal mix (10% NaOH, BW/TW = 0.9, 60 days of curing) achieved a UCS of 28.7 MPa and a Go of 30 GPa, while exhibiting minimal mass loss (<2% freeze–thaw; <3% wet–dry). Microstructural analyses revealed densified matrices and enhanced gel formation. A dimensional analysis using the Buckingham π theorem yielded a scalable predictive model that correlates material composition, alkaline activation, and curing with mechanical performance. The study underscores the feasibility of transforming CDW into durable, high-performance AAPs for sustainable infrastructure development. Full article
(This article belongs to the Section Building Materials, and Repair & Renovation)
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17 pages, 954 KB  
Article
Transportation Link Risk Analysis Through Stochastic Link Fundamental Flow Diagram
by Orlando Giannattasio and Antonino Vitetta
Future Transp. 2025, 5(4), 150; https://doi.org/10.3390/futuretransp5040150 - 21 Oct 2025
Viewed by 166
Abstract
This paper proposes a method for assessing societal risk along a traffic link by integrating a stochastic formulation of the fundamental diagram. The approach accounts for uncertainty in vehicle speed due to user heterogeneity, vehicle characteristics, and environmental conditions. The risk index is [...] Read more.
This paper proposes a method for assessing societal risk along a traffic link by integrating a stochastic formulation of the fundamental diagram. The approach accounts for uncertainty in vehicle speed due to user heterogeneity, vehicle characteristics, and environmental conditions. The risk index is decomposed into occurrence, vulnerability, and exposure components, with the occurrence probability modeled as a function of stochastic speed. The inverse gamma distribution is adopted to represent speed variability, enabling analytical tractability and control over dispersion. Numerical results show that urban and suburban environments exhibit distinct sensitivity to model parameters, particularly the gamma shape parameter η and the composite parameter c = β · v0 obtained by the product of the occurrence parameter β and the free speed flow v0. Graphical representations illustrate the impact of uncertainty on risk estimation. The proposed framework enhances existing deterministic methods by incorporating probabilistic elements, offering a foundation for future applications in traffic safety management and infrastructure design. Full article
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30 pages, 2907 KB  
Systematic Review
Health System Determinants of Delivery and Uptake of HPV Vaccination Services Among Involuntary Migrant Populations: A Qualitative Systematic Review
by Jennifer Nyawira Githaiga, Jill Olivier, Susanne Noll and Edina Amponsah-Dacosta
Vaccines 2025, 13(10), 1064; https://doi.org/10.3390/vaccines13101064 - 18 Oct 2025
Viewed by 326
Abstract
Background: Migrant populations are commonly under-immunised relative to general populations in host countries. The evidence base on routine vaccination among migrant children suggests that higher priority is given to infants and younger children compared to adolescents. Though migrants are often classified as a [...] Read more.
Background: Migrant populations are commonly under-immunised relative to general populations in host countries. The evidence base on routine vaccination among migrant children suggests that higher priority is given to infants and younger children compared to adolescents. Though migrants are often classified as a homogenous group, different sub-populations of migrants exist, including voluntary migrants who choose to move and involuntary migrants forcibly displaced by humanitarian crises. The human papillomavirus (HPV) vaccine, a relatively recent addition to global routine immunisation schedules for adolescents, is a useful proxy for understanding vaccine equity for this under-prioritised group. This qualitative systematic review explores health system determinants of delivery and uptake of HPV vaccination services among involuntary migrants. Methods: A literature search was conducted across ten electronic databases. An analytical framework tailored to the migrant context aided in capturing the complexity and magnitude of systemic factors that determine vaccine delivery and uptake among involuntary migrants. Of the 676 records retrieved, 27 studies were included in this review. Results: Key determinants of vaccine delivery include adaptation of immunisation policies for migrant inclusiveness, implementation of migrant-targeted interventions, health provider recommendations, electronic health records, and free vaccines. Uptake determinants include access dependent on legal status, awareness-related determinants akin to culturally appropriate health messaging, and acceptance-related determinants associated with sociocultural beliefs, misinformation, and distrust. Conclusions: Prioritising vaccination programmes linked with non-outbreak-related diseases is challenging in the disruptive context of humanitarian crises given fragile health systems, limited resources, loss of health infrastructure and deployment of health personnel to emergency care. We strongly advocate for global actors at all health systems levels to actively reform national HPV vaccination programmes to enhance inclusivity of adolescent girls in crises settings or resettled in host countries. Full article
(This article belongs to the Special Issue Inequality in Immunization 2025)
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27 pages, 1246 KB  
Review
Hydrogen Safety in Energy Infrastructure: A Review
by Eva Gregorovičová and Jiří Pospíšil
Energies 2025, 18(20), 5470; https://doi.org/10.3390/en18205470 - 17 Oct 2025
Viewed by 446
Abstract
For the transition to emission-free or low-emission energy, hydrogen is a promising energy carrier and fuel of the future with the possibility of long-term storage. Due to its specific properties, it poses certain safety risks; therefore, it is necessary to have a comprehensive [...] Read more.
For the transition to emission-free or low-emission energy, hydrogen is a promising energy carrier and fuel of the future with the possibility of long-term storage. Due to its specific properties, it poses certain safety risks; therefore, it is necessary to have a comprehensive understanding of hydrogen. This review article contains ten main chapters and provides, by synthesizing current findings primarily from standards and scientific studies (predominantly from 2023 to 2024), the theoretical basis for further research directed toward safe hydrogen infrastructure. Full article
(This article belongs to the Special Issue Improving Hydrogen Safety for Energy Applications)
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17 pages, 2172 KB  
Article
Brain Booster Buildings: Modelling Stair Use as a Daily Booster of Brain-Derived Neurotrophic Factor
by Mohamed Hesham Khalil and Koen Steemers
Buildings 2025, 15(20), 3730; https://doi.org/10.3390/buildings15203730 - 16 Oct 2025
Viewed by 405
Abstract
This paper establishes the Brain Booster Buildings framework, the first model to demonstrate how daily stair use can elevate brain-derived neurotrophic factor (BDNF), a vital molecule for lifelong neurogenesis and brain health in humans. Through a novel framework of the associations between metabolic [...] Read more.
This paper establishes the Brain Booster Buildings framework, the first model to demonstrate how daily stair use can elevate brain-derived neurotrophic factor (BDNF), a vital molecule for lifelong neurogenesis and brain health in humans. Through a novel framework of the associations between metabolic equivalents (METs) data and BDNF response studies, we establish that stairs are generally higher in METs than any indoor activity. We further explain how architectural parameters (riser height, floor number, pace) predictably modulate exercise intensity during stair use. We identify two implementable patterns: moderate-intensity continuous use (≥20 min, 1–3 floors) and high-intensity interval training (6 min, carrying loads while using stairs in a building with three floors or less, or using stairs in a building with ≥3 floors, load-free). Based on BDNF responses to comparable exercise intensities, 6 min of high-intensity stair climbing is predicted to increase serum BDNF by up to 40%. Since people spend ~90% of their time indoors while neurogenesis declines fourfold throughout the adult lifespan, affecting mood, stress resilience, and memory, vertical architecture emerges as a vital, accessible, and cost-effective infrastructure that boosts BDNF for neurogenesis, plasticity, and brain health. We conducted scenario-based modelling using the Brain Booster Buildings framework to estimate how the use of stairs in residential, office, educational, hospital, and commercial buildings may boost BDNF levels based on established intensity–BDNF relationships. The framework provides architects, policymakers, and clinicians with evidence-based estimated specifications to use buildings as daily brain boosters. Full article
(This article belongs to the Special Issue BioCognitive Architectural Design)
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24 pages, 2699 KB  
Article
Digital Twin Framework for Energy Transition in Gas Networks Based on Open-Source Tools: Methodology and Case Study in Southern Italy
by Filippo Luca Alberto Munafò, Ben Alex Baby, Tancredi Testasecca, Marco Ferraro and Marco Beccali
Energies 2025, 18(20), 5434; https://doi.org/10.3390/en18205434 - 15 Oct 2025
Viewed by 221
Abstract
The ongoing digitalization of energy infrastructure is a crucial enabler for improving efficiency, reliability, and sustainability in gas distribution networks, especially in the context of decarbonization and the integration of alternative energy carriers (e.g., renewable gases including biogas, green hydrogen). This study presents [...] Read more.
The ongoing digitalization of energy infrastructure is a crucial enabler for improving efficiency, reliability, and sustainability in gas distribution networks, especially in the context of decarbonization and the integration of alternative energy carriers (e.g., renewable gases including biogas, green hydrogen). This study presents the development and application of a Digital Twin framework for a real-world gas distribution network developed using open-source tools. The proposed methodology covers the entire digital lifecycle: from data acquisition through smart meters and GIS mapping, to 3D modelling and simulation using tools such as QGIS, FreeCAD, and GasNetSim. Consumption data are collected, processed, and harmonized via Python-based workflows, hourly simulations of network operation, including pressure, flow rate, and gas quality indicators like the Wobbe Index. Results demonstrate the effectiveness of the Digital Twin in accurately replicating real network behavior and supporting scenario analyses for the introduction of greener energy vectors such as hydrogen or biomethane. The case study highlights the flexibility and transparency of the workflow, as well as the critical importance of data quality and availability. The framework provides a robust basis for advanced network management, optimization, and planning, offering practical tools to support the energy transition in the gas sector. Full article
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15 pages, 1996 KB  
Article
Implementation of Acyclic Matching in Aerospace Technology for Honeycomb-Designed Satellite Constellations
by Saffren Sundher, Angel Dharmakkan, Govindarajan Arunachalam, Vidhya Mohanakrishnan and Manigandan Sekar
Mathematics 2025, 13(20), 3280; https://doi.org/10.3390/math13203280 - 14 Oct 2025
Viewed by 215
Abstract
Operational satellites are critical to modern aerospace infrastructure, supporting essential services such as communication, navigation, and global surveillance. However, the increasing density of satellites and space debris in Earth’s orbit has heightened the risk of collisions, thereby threatening network reliability. This study addresses [...] Read more.
Operational satellites are critical to modern aerospace infrastructure, supporting essential services such as communication, navigation, and global surveillance. However, the increasing density of satellites and space debris in Earth’s orbit has heightened the risk of collisions, thereby threatening network reliability. This study addresses the dual challenge of managing space debris and enhancing satellite network performance by applying the concept of acyclic matching from graph theory to satellite constellations modeled as honeycomb networks. Acyclic matching identifies edge subsets without shared nodes or cycles, enabling static signal rerouting through pre-computed, loop-free paths. This ensures fault tolerance and efficient resource allocation in increasingly complex satellite constellations. The proposed method derives the general solution for acyclic matching cardinality and determines the maximum matching set for n-dimensional honeycomb networks. This technique aligns with emerging trends in autonomous fault-tolerant systems and adaptive routing protocols, proving particularly relevant for large-scale satellite systems such as Starlink and global navigation constellations. By providing alternative communication paths in the event of satellite or link failures, the approach significantly enhances the scalability, reliability, and resilience of satellite networks, ensuring uninterrupted service and improved space traffic management in the face of rising orbital congestion. Full article
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17 pages, 4515 KB  
Article
Mitigation of Expansive Soil Through Controlled Thermal Treatment: Geotechnical and Microstructural Assessment
by Abdullah Alsabhan, Wagdi Hamid, Ahmed M. Al-Mahbashi and Abobaker Salem Binyahya
Buildings 2025, 15(20), 3678; https://doi.org/10.3390/buildings15203678 - 13 Oct 2025
Viewed by 266
Abstract
Expansive soils present a significant geotechnical challenge due to their pronounced volume changes with moisture variations, leading to substantial infrastructure damage. This study investigates the efficacy of thermal stabilization in mitigating the swell potential and compressibility of a high-plasticity, kaolinite-rich clay from Al [...] Read more.
Expansive soils present a significant geotechnical challenge due to their pronounced volume changes with moisture variations, leading to substantial infrastructure damage. This study investigates the efficacy of thermal stabilization in mitigating the swell potential and compressibility of a high-plasticity, kaolinite-rich clay from Al Ghat, Saudi Arabia. As well, the changes in basic properties including consistency limits, specific gravity, and compaction characteristics were studied and highlighted. Microstructural studies using X-ray diffraction (XRD), Scanning electron microscopy (SEM), and Energy-dispersive X-ray spectroscopic (EDX) were performed to trace the structural changes and interpret the achieved improvement. Soil specimens were subjected to heat treatment at levels of 200 °C, 400 °C, and 600 °C for two hours, after which their geotechnical and microstructural properties were comprehensively evaluated. The results demonstrate a direct correlation between increasing temperature and the reduction in expansive behavior. Treatment at 600 °C caused a substantial decrease in the plasticity index from 27.00 to 2.94. Correspondingly, oedometer tests showed that the free swell was reduced from 6% to nearly zero, and the swelling pressure was eliminated, dropping from 250 kPa to 0 kPa. XRD analysis confirmed kaolinite decomposition through dehydroxylation, producing metakaolin with diminished water absorption capacity. SEM further revealed significant particle aggregation and the formation of a coarser soil fabric. The findings confirm that heat treatment at temperatures of 400 °C and above is a highly effective method for permanently stabilizing kaolinitic expansive soils, rendering them suitable for construction applications. Full article
(This article belongs to the Special Issue Research on Soil–Structure Interaction for Civil Structures)
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14 pages, 1214 KB  
Article
Microwave-Enabled Two-Step Scheme for Continuous Variable Quantum Communications in Integrated Superconducting
by Yun Mao, Lei Mao, Wanyi Wang, Yijun Wang, Hang Zhang and Ying Guo
Mathematics 2025, 13(20), 3263; https://doi.org/10.3390/math13203263 - 12 Oct 2025
Viewed by 174
Abstract
Quantum secure direct communication (QSDC) is convenient for the direct transmission of secure messages without requiring a prior key exchange by two participants, offering an elegant advantage in transmission security. The traditional implementations usually focus on the discrete-variable (DV) system, whereas its continuous-variable [...] Read more.
Quantum secure direct communication (QSDC) is convenient for the direct transmission of secure messages without requiring a prior key exchange by two participants, offering an elegant advantage in transmission security. The traditional implementations usually focus on the discrete-variable (DV) system, whereas its continuous-variable (CV) counterpart has attracted much attention due to its compatibility with existing optical infrastructure. In order to address its practical deployment in harsh environments, we propose a microwave-based scheme for the CV-QSDC that leverages entangled microwave quantum states through free-space channels in cryogenic environments. The two-step scheme is designed for the secure direct communication, where the classical messages can be encoded by using Gaussian modulation and then transmitted via displacement operations on microwave quantum states. The data processing procedures involve microwave entangled state generation, channel detection, parameter estimation, and so on. Simulation results demonstrate the feasibility of the microwave-based CV-QSDC, highlighting its potential for secure communication in integrated superconducting and solid-state quantum technologies. Full article
(This article belongs to the Special Issue Quantum Information, Cryptography and Computation)
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14 pages, 2291 KB  
Article
Infrared FEL-Induced Alteration of Zeta Potential in Electrochemically Grown Quantum Dots: Insights into Ion Modification
by Sukrit Sucharitakul, Siripatsorn Thanasanvorakun, Vasan Yarangsi, Suparoek Yarin, Kritsada Hongsith, Monchai Jitvisate, Hideaki Ohgaki, Surachet Phadungdhitidhada, Heishun Zen, Sakhorn Rimjaem and Supab Choopun
Nanomaterials 2025, 15(20), 1543; https://doi.org/10.3390/nano15201543 - 10 Oct 2025
Viewed by 611
Abstract
This study explores the use of mid-infrared (MIR) free-electron laser (FEL) irradiation as a tool for tailoring the surface properties of electrochemically synthesized TiO2—graphene quantum dots (QDs). The QDs, prepared in colloidal form via a cost-effective electrochemical method in a KCl—citric [...] Read more.
This study explores the use of mid-infrared (MIR) free-electron laser (FEL) irradiation as a tool for tailoring the surface properties of electrochemically synthesized TiO2—graphene quantum dots (QDs). The QDs, prepared in colloidal form via a cost-effective electrochemical method in a KCl—citric acid medium, were exposed to MIR wavelengths (5.76, 8.02, and 9.10 µm) at the Kyoto University FEL facility. Post-irradiation measurements revealed a pronounced inversion of zeta potential by 40–50 mV and approximately 10% reduction in hydrodynamic size, indicating double-layer contraction and ionic redistribution at the QD—solvent interface. Photoluminescence spectra showed enhanced emission for GQDs and TiO2/GQD composites, while Tauc analysis revealed modest bandgap blue shifts (0.04–0.08 eV), both consistent with trap-state passivation and sharper band edges. TEM confirmed intact crystalline structures, verifying that FEL-induced modifications were confined to surface chemistry rather than bulk lattice damage. Taken together, these results demonstrate that MIR FEL irradiation provides a resonance-driven, non-contact method to reorganize ions, suppress defect states, and improve the optoelectronic quality of QDs. This approach offers a scalable post-synthetic pathway for enhancing electron transport layers in perovskite solar cells and highlights the broader potential of photonic infrastructure for advanced nanomaterial processing and interface engineering in optoelectronic and energy applications. Full article
(This article belongs to the Section Nanoelectronics, Nanosensors and Devices)
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17 pages, 2143 KB  
Article
CRISPR-Cas12a-Based Isothermal Detection of Mammarenavirus machupoense Virus: Optimization and Evaluation of Multiplex Capability
by Marina A. Kapitonova, Anna V. Shabalina, Vladimir G. Dedkov and Anna S. Dolgova
Int. J. Mol. Sci. 2025, 26(19), 9754; https://doi.org/10.3390/ijms26199754 - 7 Oct 2025
Viewed by 413
Abstract
Bolivian hemorrhagic fever (BHF) is a zoonotic disease caused by Mammarenavirus machupoense (MACV) featuring severe neurological and hemorrhagic symptoms and a high mortality rate. BHF is usually diagnosed by serological tests or real-time polymerase chain reaction (RT-PCR); these methods are often inaccessible in [...] Read more.
Bolivian hemorrhagic fever (BHF) is a zoonotic disease caused by Mammarenavirus machupoense (MACV) featuring severe neurological and hemorrhagic symptoms and a high mortality rate. BHF is usually diagnosed by serological tests or real-time polymerase chain reaction (RT-PCR); these methods are often inaccessible in endemic regions due to a lack of laboratory infrastructure, creating a demand for sensitive and rapid equipment-free alternatives. Here, we present an isothermal method for MACV nucleic acid detection based on the Cas12a-based DETECTR system combined with recombinase polymerase amplification (RPA) in a single tube: the RT-RPA/DETECTR assay. We demonstrate the possibility of using more than one primer set for the simultaneous detection of MACV genetic variants containing multiple point mutations. The method was optimized and tested using specially developed virus-like armored particles containing the target sequence. The multiplex RT-RPA/DETECTR method achieved a limit of detection of approximately 5 × 104 copies/ mL (80 aM) of armored particles. The method was validated using clinical samples spiked with virus-like particles. The assay proved to be selective and reliable in detecting certain nucleotide substitutions simultaneously. Full article
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32 pages, 19967 KB  
Article
Monitoring the Recovery Process After Major Hydrological Disasters with GIS, Change Detection and Open and Free Multi-Sensor Satellite Imagery: Demonstration in Haiti After Hurricane Matthew
by Wilson Andres Velasquez Hurtado and Deodato Tapete
Water 2025, 17(19), 2902; https://doi.org/10.3390/w17192902 - 7 Oct 2025
Viewed by 507
Abstract
Recovery from disasters is the complex process requiring coordinated measures to restore infrastructure, services and quality of life. While remote sensing is a well-established means for damage assessment, so far very few studies have shown how satellite imagery can be used by technical [...] Read more.
Recovery from disasters is the complex process requiring coordinated measures to restore infrastructure, services and quality of life. While remote sensing is a well-established means for damage assessment, so far very few studies have shown how satellite imagery can be used by technical officers of affected countries to provide crucial, up-to-date information to monitor the reconstruction progress and natural restoration. To address this gap, the present study proposes a multi-temporal observatory method relying on GIS, change detection techniques and open and free multi-sensor satellite imagery to generate thematic maps documenting, over time, the impact and recovery from hydrological disasters such as hurricanes, tropical storms and induced flooding. The demonstration is carried out with regard to Hurricane Matthew, which struck Haiti in October 2016 and triggered a humanitarian crisis in the Sud and Grand’Anse regions. Synthetic Aperture Radar (SAR) amplitude change detection techniques were applied to pre-, cross- and post-disaster Sentinel-1 image pairs from August 2016 to September 2020, while optical Sentinel-2 images were used for verification and land cover classification. With regard to inundated areas, the analysis allowed us to determine the needed time for water recession and rural plain areas to be reclaimed for agricultural exploitation. With regard to buildings, the cities of Jérémie and Les Cayes were not only the most impacted areas, but also were those where most reconstruction efforts were made. However, some instances of new settlements located in at-risk zones, and thus being susceptible to future hurricanes, were found. This result suggests that the thematic maps can support policy-makers and regulators in reducing risk and making the reconstruction more resilient. Finally, to evaluate the replicability of the proposed method, an example at a country-scale is discussed with regard to the June 2023 flooding event. Full article
(This article belongs to the Special Issue Applications of GIS and Remote Sensing in Hydrology and Hydrogeology)
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